Invitrogen

FOXP3 Monoclonal Antibody (FJK-16s), Alexa Fluor™ 488, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

43 References

View all (163) FOXP3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

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FOXP3 Antibody (53-5773-82) in Flow

C57BL/6 mouse splenocytes were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD4 Monoclonal Antibody, APC (Product # 17-0042-82) and 0.125 µg of Mouse IgG2a kappa Isotype Control, Alexa Fluor 488 (Product # 53-4724-80) (left) or 0.125 µg of FOXP3 Monoclonal Antibody, Alexa Fluor 488 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

FOXP3 Antibody — Intracellular staining of mouse splenocytes. As expected based on known relative expression patterns, Foxp3 clone FJK-16s stains a subset of the CD4+ T cells and does not stain the CD8+ T cells. Details: Balb/c splenocytes were surface stained with CD3 (clone 17A2), CD4 (clone GK1.5) and CD8 (clone 53-6.7), followed by intracellular staining with Foxp3 (clone FJK-16s) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD3+CD8+ (blue histogram) and CD3+CD4+ (purple histogram) gates were used for analysis. {RE}

Product Details

53-5773-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 39 publications 39 publications

Product Specifications

Species Reactivity

Bovine, Dog, Cat, Mouse, Pig, Rat

Published species

Bovine, Mouse, Rat

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

FJK-16s

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_763537

Product Specific Information

Description: The FJK-16s antibody reacts with mouse, rat, dog, porcine, bovine and cat Foxp3 also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of foxP3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Immunoblotting with FJK-16s antibody has mapped the epitope to amino acids 75-125 of the mouse Foxp3 protein. In the human, this region has been shown to be alternatively spliced at the mRNA level. Both the alternatively-spliced and non-spliced isoforms are present in the CD4+CD25+ subset of lymphocytes. Preliminary RT-PCR experiments have not revealed this alternatively-spliced isoform in mouse splenocytes, suggesting different gene regulation in the mouse and human.

Please note that FJK-16s has been optimized for use with the Foxp3/Transcription Factor Buffer Staining Set (Product # 00-5523-00). The use of other fixation and staining buffers is not recommended.

Applications Reported: This FJK-16s antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This FJK-16s antibody has been tested by intracellular staining and flow cytometric analysis of mouse splenocytes using Foxp3/Transcription Factor Buffer Set (Product # 00-5523-00) and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Epithelial cell-derived cytokine TSLP activates regulatory T cells by enhancing fatty acid uptake.

Scientific reports

Kasuya T,Tanaka S,Tamura J,Etori K,Shoda J,Hattori K,Endo Y,Kitajima M,Kageyama T,Iwamoto T,Yokota M,Iwata A,Suto A,Suzuki K,Suzuki H,Ziegler SF,Nakajima H

53-5773-82 was used in Flow cytometry/Cell sorting to show that TSLP modulates the activation status of induced Treg through the enhanced uptake of fatty acids to maintain homeostasis in the large intestine.

Mon Jan 30 00:00:00 EST 2023

Context-dependent effects of IL-2 rewire immunity into distinct cellular circuits.

The Journal of experimental medicine

Whyte CE,Singh K,Burton OT,Aloulou M,Kouser L,Veiga RV,Dashwood A,Okkenhaug H,Benadda S,Moudra A,Bricard O,Lienart S,Bielefeld P,Roca CP,Naranjo-Galindo FJ,Lombard-Vadnais F,Junius S,Bending D,Ono MM,Hochepied T,Halim TYF,Schlenner S,Lesage S,Dooley J,Liston A

53-5773-82 was used in Immunohistochemistry-immunofluorescence to support that targeted IL-2 production has the potential to amplify or quench particular circuits in the IL-2 network, based on clinical desirability.

Mon Jul 04 00:00:00 EDT 2022

Neoantigen vaccine-induced CD4 T cells confer protective immunity in a mouse model of multiple myeloma through activation of CD8 T cells against non-vaccine, tumor-associated antigens.

Journal for immunotherapy of cancer

Bekri S,Rodney-Sandy R,Gruenstein D,Mei A,Bogen B,Castle J,Levey D,Cho HJ

53-5773-82 was used in Flow cytometry/Cell sorting to indicate that activation of CD8 CTL against MOPC315-associated antigens not present in the vaccine is one of the major mechanisms of tumor immunity.

Tue Feb 01 00:00:00 EST 2022

Protocol to evaluate cell lineage stability of mouse natural and induced regulatory T cells using bisulfite sequencing.

STAR protocols

Arai M,Fukuda A,Morimoto R,Nakamura Y,Ci Z,Sakaguchi S

53-5773-82 was used in Immunocytochemistry to assess Treg-lineage stability.

Fri Dec 16 00:00:00 EST 2022

Preventing type 1 diabetes in late-stage pre-diabetic NOD mice with insulin: A central role for alum as adjuvant.

Frontiers in endocrinology

Martens PJ,Ellis D,Bruggeman Y,Viaene M,Laureys J,Teyton L,Mathieu C,Gysemans C

53-5773-82 was used in Flow cytometry/Cell sorting to support the use of alum as adjuvant to optimise the efficacy of antigen-specific immunotherapy in future trials.

Tue Nov 08 00:00:00 EST 2022

Ubiquitin-specific peptidase 18 regulates the differentiation and function of Treg cells.

Genes & diseases

Yang L,Jing Y,Kang D,Jiang P,Li N,Zhou X,Chen Y,Westerberg LS,Liu C

53-5773-82 was used in Flow Cytometry to identify the role of USP18 in Tregs differentiation and its suppressive function.

Sat May 01 00:00:00 EDT 2021

Metabolic support of tumour-infiltrating regulatory T cells by lactic acid.

Nature

Watson MJ,Vignali PDA,Mullett SJ,Overacre-Delgoffe AE,Peralta RM,Grebinoski S,Menk AV,Rittenhouse NL,DePeaux K,Whetstone RD,Vignali DAA,Hand TW,Poholek AC,Morrison BM,Rothstein JD,Wendell SG,Delgoffe GM

53-5773-82 was used in Flow cytometry/Cell sorting to suggest that tumours avoid destruction by not only depriving effector T cells of nutrients, but also metabolically supporting regulatory populations.

Mon Mar 01 00:00:00 EST 2021

Regulatory T-cells inhibit microglia-induced pain hypersensitivity in female mice.

eLife

Kuhn JA,Vainchtein ID,Braz J,Hamel K,Bernstein M,Craik V,Dahlgren MW,Ortiz-Carpena J,Molofsky AB,Molofsky AV,Basbaum AI

53-5773-82 was used in Flow Cytometry to conclude that sexual dimorphism in the contribution of microglia to pain results from Treg-mediated suppression of microglial activation and pain hypersensitivity in female mice.

Fri Oct 15 00:00:00 EDT 2021

CCL2 regulation of MST1-mTOR-STAT1 signaling axis controls BCR signaling and B-cell differentiation.

Cell death and differentiation

Yang L,Li N,Yang D,Chen A,Tang J,Jing Y,Kang D,Jiang P,Dai X,Luo L,Chen Q,Chang J,Liu J,Gu H,Huang Y,Chen Q,Li Z,Zhu Y,Miller H,Chen Y,Qiu L,Mei H,Hu Y,Gong Q,Liu C

53-5773-82 was used in flow cytometry to reveal the intrinsic role and underlying molecular mechanism of CCL2 in BCR signaling, B-cell differentiation, and humoral response.

Wed Sep 01 00:00:00 EDT 2021

Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease.

Cell

Lee JY,Hall JA,Kroehling L,Wu L,Najar T,Nguyen HH,Lin WY,Yeung ST,Silva HM,Li D,Hine A,Loke P,Hudesman D,Martin JC,Kenigsberg E,Merad M,Khanna KM,Littman DR

53-5773 was used in Flow cytometry/Cell sorting to suggest that serum amyloid A proteins direct a pathogenic pro-inflammatory Th17 cell differentiation program and promote Th17-mediated inflammatory diseases.

Thu Jan 09 00:00:00 EST 2020

Niche-Selective Inhibition of Pathogenic Th17 Cells by Targeting Metabolic Redundancy.

Cell

Wu L,Hollinshead KER,Hao Y,Au C,Kroehling L,Ng C,Lin WY,Li D,Silva HM,Shin J,Lafaille JJ,Possemato R,Pacold ME,Papagiannakopoulos T,Kimmelman AC,Satija R,Littman DR

53-5773-82 was used in Flow Cytometry to suggest that inhibiting glycolysis by targeting Gpi1 could be an effective therapeutic strategy with minimum toxicity for Th17-mediated autoimmune diseases, and, more generally, that metabolic redundancies can be exploited for selective targeting of disease processes.

Thu Aug 06 00:00:00 EDT 2020

Plasmacytoid Dendritic Cells and Type I Interferon Promote Extrafollicular B Cell Responses to Extracellular Self-DNA.

Immunity

Soni C,Perez OA,Voss WN,Pucella JN,Serpas L,Mehl J,Ching KL,Goike J,Georgiou G,Ippolito GC,Sisirak V,Reizis B

53-5773-82 was used in Flow Cytometry to establish extrafollicular B cell differentiation into short-lived AFCs as a key mechanism of anti-DNA autoreactivity and reveal a major contribution of pDCs, endosomal Toll-like receptors (TLRs), and IFN-I to this pathway.

Tue Jun 16 00:00:00 EDT 2020

Transplantation tolerance modifies donor-specific B cell fate to suppress de novo alloreactive B cells.

The Journal of clinical investigation

Khiew SH,Jain D,Chen J,Yang J,Yin D,Young JS,Dent A,Sciammas R,Alegre ML,Chong AS

53-5773 was used in Flow cytometry/Cell sorting to showed that alloreactive B cells were not deleted but rapidly lost their ability to differentiate into germinal center B cells and secrete donor-specific antibodies., by using a murine model of heart tolerance.

Wed Jul 01 00:00:00 EDT 2020

Polymicrobial Sepsis Impairs Antigen-Specific Memory CD4 T Cell-Mediated Immunity.

Frontiers in immunology

Sjaastad FV,Kucaba TA,Dileepan T,Swanson W,Dail C,Cabrera-Perez J,Murphy KA,Badovinac VP,Griffith TS

53-5773-82 was used in Flow Cytometry to show CLP-induced sepsis alters the number and function of Ag-specific memory CD4 T cells, which contributes (in part) to the characteristic long-lasting immunoparalysis seen after sepsis.

Mon Apr 19 00:00:00 EDT 2021

STING couples with PI3K to regulate actin reorganization during BCR activation.

Science advances

Jing Y,Dai X,Yang L,Kang D,Jiang P,Li N,Cheng J,Li J,Miller H,Ren B,Gong Q,Yin W,Liu Z,Mattila PK,Ning Q,Sun J,Yu B,Liu C

53-5773 was used in Flow cytometry/Cell sorting to study the effect of STING (stimulator of interferon genes) deficiency on B cell development, differentiation, and BCR signalling.

Wed Apr 01 00:00:00 EDT 2020

Continued Bcl6 Expression Prevents the Transdifferentiation of Established Tfh Cells into Th1 Cells during Acute Viral Infection.

Cell reports

Alterauge D,Bagnoli JW,Dahlström F,Bradford BM,Mabbott NA,Buch T,Enard W,Baumjohann D

53-5773-82 was used in Flow Cytometry to evaluate the contributions of the CXCR5 receptor and Bcl6 transcription factor in maintaining T follicular helper cells and germinal centres.

Tue Oct 06 00:00:00 EDT 2020

Natural killer cells modulate motor neuron-immune cell cross talk in models of Amyotrophic Lateral Sclerosis.

Nature communications

Garofalo S,Cocozza G,Porzia A,Inghilleri M,Raspa M,Scavizzi F,Aronica E,Bernardini G,Peng L,Ransohoff RM,Santoni A,Limatola C

53-5773 was used in Immunohistochemistry-immunofluorescence to study the role of NK cells in determining the onset and progression of motor neuron degeneration in Amyotrophic Lateral Sclerosis.

Tue Apr 14 00:00:00 EDT 2020

Osteoprotegerin-dependent M cell self-regulation balances gut infection and immunity.

Nature communications

Kimura S,Nakamura Y,Kobayashi N,Shiroguchi K,Kawakami E,Mutoh M,Takahashi-Iwanaga H,Yamada T,Hisamoto M,Nakamura M,Udagawa N,Sato S,Kaisho T,Iwanaga T,Hase K

53-5773 was used in Flow cytometry/Cell sorting to identify that the balance between infectious risk and the ability to perform immunosurveillance at the mucosal surface is dependent on osteprotegerin-dependent self-regulation of M cell differentiation.

Mon Jan 13 00:00:00 EST 2020

Drp1 Controls Effective T Cell Immune-Surveillance by Regulating T Cell Migration, Proliferation, and cMyc-Dependent Metabolic Reprogramming.

Cell reports

Simula L,Pacella I,Colamatteo A,Procaccini C,Cancila V,Bordi M,Tregnago C,Corrado M,Pigazzi M,Barnaba V,Tripodo C,Matarese G,Piconese S,Campello S

53-5773 was used in Flow cytometry/Cell sorting to describe the crucial role of the mitochondrial protein Drp1 in T cell development and regulation of lymphocyte immune-surveillance.

Tue Dec 11 00:00:00 EST 2018

Differential cell-intrinsic regulations of germinal center B and T cells by miR-146a and miR-146b.

Nature communications

Cho S,Lee HM,Yu IS,Choi YS,Huang HY,Hashemifar SS,Lin LL,Chen MC,Afanasiev ND,Khan AA,Lin SW,Rudensky AY,Crotty S,Lu LF

53-5773 was used in Flow cytometry/Cell sorting to reveal differential cell-intrinsic regulations of GC B and Tfh cells by miR-146a and miR-146b.

Mon Jul 16 00:00:00 EDT 2018

Differential Roles of IL-2 Signaling in Developing versus Mature Tregs.

Cell reports

Fan MY,Low JS,Tanimine N,Finn KK,Priyadharshini B,Germana SK,Kaech SM,Turka LA

53-5773 was used in Flow cytometry/Cell sorting to study the differential roles of IL-2 signalling in developing versus mature regulatory T cells.

Tue Oct 30 00:00:00 EDT 2018

IRF-5 Promotes Cell Death in CD4 T Cells during Chronic Infection.

Cell reports

Fabié A,Mai LT,Dagenais-Lussier X,Hammami A,van Grevenynghe J,Stäger S

53-5773 was used in Flow cytometry/Cell sorting to demonstrate that IRF-5 is increasingly expressed in interferon (IFN)-γ+ CD4 T cells over the course of L. donovani infection.

Tue Jul 31 00:00:00 EDT 2018

Analyses of a Mutant Foxp3 Allele Reveal BATF as a Critical Transcription Factor in the Differentiation and Accumulation of Tissue Regulatory T Cells.

Immunity

Hayatsu N,Miyao T,Tachibana M,Murakami R,Kimura A,Kato T,Kawakami E,Endo TA,Setoguchi R,Watarai H,Nishikawa T,Yasuda T,Yoshida H,Hori S

53-5773 was used in Flow cytometry/Cell sorting to identify BATF as a regulator of tissue regulatory T cells and suggest that sequence-specific perturbations of Foxp3-DNA interactions can influence specific facets of Treg physiology and the immunopathologies.

Tue Aug 15 00:00:00 EDT 2017

Huai Qi Huang corrects the balance of Th1/Th2 and Treg/Th17 in an ovalbumin-induced asthma mouse model.

Bioscience reports

Liang P,Peng S,Zhang M,Ma Y,Zhen X,Li H

53-5773 was used in Flow cytometry/Cell sorting to determine the immunoregulatory effects of Huai Qi Huang on regulatory and helper T cell balance in ovalbumin induced asthma.

Fri Dec 22 00:00:00 EST 2017

Toxoplasma Co-infection Prevents Th2 Differentiation and Leads to a Helminth-Specific Th1 Response.

Frontiers in cellular and infection microbiology

Ahmed N,French T,Rausch S,Kühl A,Hemminger K,Dunay IR,Steinfelder S,Hartmann S

53-5773 was used in Flow cytometry/Cell sorting to conclude a previous T. gondii infection limits a helminth-specific Th2 immune response while promoting a shift toward a Th1-type immune response.

Mon Apr 02 00:00:00 EDT 2018

Group 2 innate lymphoid cells license dendritic cells to potentiate memory TH2 cell responses.

Nature immunology

Halim TY,Hwang YY,Scanlon ST,Zaghouani H,Garbi N,Fallon PG,McKenzie AN

53-5773 was used in Flow cytometry/Cell sorting to investigate the role of ILC2 cells in allergic inflammation, showing that they license dendritic cells to potentiate memory TH2 cell responses.

Fri Jan 01 00:00:00 EST 2016

Foxp3(+) T cells expressing RORγt represent a stable regulatory T-cell effector lineage with enhanced suppressive capacity during intestinal inflammation.

Mucosal immunology

Yang BH,Hagemann S,Mamareli P,Lauer U,Hoffmann U,Beckstette M,Föhse L,Prinz I,Pezoldt J,Suerbaum S,Sparwasser T,Hamann A,Floess S,Huehn J,Lochner M

53-5773 was used in Flow cytometry/Cell sorting to demonstrate that Foxp3(+) T cells expressing retinoic acid-related orphan receptor-γt, represent a stable regulatory T cell lineage with a highly suppressive phenotype in vivo.

Tue Mar 01 00:00:00 EST 2016

Neutrophils drive accelerated tumor progression in the collagen-dense mammary tumor microenvironment.

Breast cancer research : BCR

García-Mendoza MG,Inman DR,Ponik SM,Jeffery JJ,Sheerar DS,Van Doorn RR,Keely PJ

53-5773 was used in Flow cytometry/Cell sorting to suggest that tumor progression in a collagen-dense microenvironment is mechanistically different, with pro-tumor neutrophils, compared to a non-dense microenvironment.

Wed May 11 00:00:00 EDT 2016

Rapid linkage of innate immunological signals to adaptive immunity by the brain-fat axis.

Nature immunology

Kim MS,Yan J,Wu W,Zhang G,Zhang Y,Cai D

53-5773 was used in Flow cytometry/Cell sorting to investigate the role of the brain in innate immunological signals, showing rapid linkage of innate signals and adaptive immunity by the brain-fat axis.

Fri May 01 00:00:00 EDT 2015

Diverse roles for T-bet in the effector responses required for resistance to infection.

Journal of immunology (Baltimore, Md. : 1950)

Harms Pritchard G,Hall AO,Christian DA,Wagage S,Fang Q,Muallem G,John B,Glatman Zaretsky A,Dunn WG,Perrigoue J,Reiner SL,Hunter CA

53-5773 was used in Flow cytometry/Cell sorting to highlight T-bet-independent pathways to IFN-γ production and reveal a novel role for this transcription factor in coordinating the T cell responses necessary to control infection in peripheral tissues.

Sun Feb 01 00:00:00 EST 2015

Grazing dairy cows had decreased interferon-γ, tumor necrosis factor, and interleukin-17, and increased expression of interleukin-10 during the first week after calving.

Journal of dairy science

Heiser A,McCarthy A,Wedlock N,Meier S,Kay J,Walker C,Crookenden MA,Mitchell MD,Morgan S,Watkins K,Loor JJ,Roche JR

53-5773 was used in Flow cytometry/Cell sorting to highlight a characteristic expression pattern for IFNγ, tumor necrosis factor, IL-17, and IL-10 that reflects a state of reduced immunocompetence in moderate-yielding pasture-based transition cows after calving.

Sun Feb 01 00:00:00 EST 2015

Peak inflammation in atherosclerosis, primary biliary cirrhosis and autoimmune arthritis is counter-intuitively associated with regulatory T cell enrichment.

Immunobiology

Garetto S,Trovato AE,Lleo A,Sala F,Martini E,Betz AG,Norata GD,Invernizzi P,Kallikourdis M

53-5773 was used in Flow cytometry/Cell sorting to investigate the dynamic between regulatory T cell mediated inflammation and immunosuppression.

Sat Aug 01 00:00:00 EDT 2015

A Novel Transgenic Mouse Line for Tracing MicroRNA-155-5p Activity In Vivo.

PloS one

Phiwpan K,Guo J,Zhang W,Hu T,Boruah BM,Zhang J,Zhou X

53-5773 was used in Flow cytometry/Cell sorting to demonstrate a novel approach for detecting miR expression in specific cell types of interest.

Tue Apr 12 00:00:00 EDT 2016

Spironolactone decreases DOCA-salt-induced organ damage by blocking the activation of T helper 17 and the downregulation of regulatory T lymphocytes.

Hypertension (Dallas, Tex. : 1979)

Amador CA,Barrientos V,Peña J,Herrada AA,González M,Valdés S,Carrasco L,Alzamora R,Figueroa F,Kalergis AM,Michea L

53-5773 was used in Flow cytometry/Cell sorting to study the adaptive immune response-induced inflammatory mechanisms contributing to fibrosis.

Tue Apr 01 00:00:00 EDT 2014

The transcription factor E74-like factor 4 suppresses differentiation of proliferating CD4+ T cells to the Th17 lineage.

Journal of immunology (Baltimore, Md. : 1950)

Lee PH,Puppi M,Schluns KS,Yu-Lee LY,Dong C,Lacorazza HD

53-5773 was used in Flow cytometry/Cell sorting to purified cells CD4+ T cells are inhibited from differentiating into Th17 cells by E74-like Factor 4.

Wed Jan 01 00:00:00 EST 2014

Natural history of tumor growth and immune modulation in common spontaneous murine mammary tumor models.

Breast cancer research and treatment

Gad E,Rastetter L,Slota M,Koehnlein M,Treuting PM,Dang Y,Stanton S,Disis ML

53-5773 was used in Flow cytometry/Cell sorting to evaluate the relationship between growth rates of tumours in common spontaneous mammary tumor models and immune biomarkers evaluated in the tumour and blood.

Mon Dec 01 00:00:00 EST 2014

Regulatory T cells prevent Th2 immune responses and pulmonary eosinophilia during respiratory syncytial virus infection in mice.

Journal of virology

Durant LR,Makris S,Voorburg CM,Loebbermann J,Johansson C,Openshaw PJ

53-5773 was used in Flow cytometry/Cell sorting to provide evidence that dysregulation of normal immune responses to viral infection may contribute to severe respiratory viral disease.

Tue Oct 01 00:00:00 EDT 2013

The cytokines interleukin 27 and interferon-γ promote distinct Treg cell populations required to limit infection-induced pathology.

Immunity

Hall AO,Beiting DP,Tato C,John B,Oldenhove G,Lombana CG,Pritchard GH,Silver JS,Bouladoux N,Stumhofer JS,Harris TH,Grainger J,Wojno ED,Wagage S,Roos DS,Scott P,Turka LA,Cherry S,Reiner SL,Cua D,Belkaid Y,Elloso MM,Hunter CA

53-5773 was used in Flow cytometry/Cell sorting to study the promotion of distinct Treg cell populations by the cytokines IL-27 and IFN-y, and the role they play in limiting infection induced pathology.

Fri Sep 21 00:00:00 EDT 2012

Lymphocyte phenotypes in wild-caught rats suggest potential mechanisms underlying increased immune sensitivity in post-industrial environments.

Cellular & molecular immunology

Trama AM,Holzknecht ZE,Thomas AD,Su KY,Lee SM,Foltz EE,Perkins SE,Lin SS,Parker W

53-5773 was used in Flow cytometry/Cell sorting to lend insight into the pathogenesis of post-industrial epidemics of allergy and autoimmune disease.

Thu Mar 01 00:00:00 EST 2012

Negative regulation of pulmonary Th17 responses by C3a anaphylatoxin during allergic inflammation in mice.

PloS one

Lim H,Kim YU,Drouin SM,Mueller-Ortiz S,Yun K,Morschl E,Wetsel RA,Chung Y

53-5773 was used in Flow cytometry/Cell sorting to demonstrate that C3a signals negatively regulate antigen-specific Th17 responses during allergic lung inflammation and the Foxp3(+) regulatory T cell population.

Thu Jun 27 00:00:00 EDT 2013

CD4+ Foxp3+ regulatory T cells mediate Toxoplasma gondii-induced T-cell suppression through an IL-2-related mechanism but independently of IL-10.

European journal of immunology

Tenorio EP,Fernández J,Castellanos C,Olguín JE,Saavedra R

53-5773 was used in Flow cytometry/Cell sorting to study the role of Treg cells in Toxoplasma gondii induced suppression.

Thu Dec 01 00:00:00 EST 2011

Sensitive detection of Foxp3 expression in bovine lymphocytes by flow cytometry.

Veterinary immunology and immunopathology

Gerner W,Stadler M,Hammer SE,Klein D,Saalmüller A

Mon Nov 15 00:00:00 EST 2010

Age-dependent loss of tolerance to an immunodominant epitope of glutamic acid decarboxylase in diabetic-prone RIP-B7/DR4 mice.

Clinical immunology (Orlando, Fla.)

Gebe JA,Unrath KA,Falk BA,Ito K,Wen L,Daniels TL,Lernmark A,Nepom GT

53-5773 was used in Immunohistochemistry on frozen tissues to identify an age-dependent spontaneous loss of tolerance to two self-antigenic epitopes derived from putative diabetes-associated antigens in HLA specific transgenic mice.

Fri Dec 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: Forkhead box protein P3; forkhead/winged helix transcription factor 3; Foxp3; MGC141961; MGC141963; RP23-54C14.1; Scurfin; scurfy

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Gene Aliases: Foxp3; JM2; RGD1562112; scurfin; sf

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UniProt ID: (Mouse) Q99JB6

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Entrez Gene ID: (Dog) 491876, (Pig) 444998, (Bovine) 506053, (Cat) 100037405, (Mouse) 20371, (Rat) 317382

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Function(s)

core promoter binding nucleic acid binding DNA binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NFAT protein binding RNA polymerase II transcription factor activity, sequence-specific DNA binding molecular_function

Process(es)

Performance Guarantee

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FOXP3 Monoclonal Antibody (FJK-16s), Alexa Fluor™ 488, eBioscience™

FOXP3 Antibody (53-5773-82) in Flow

Product Details

53-5773-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

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