Invitrogen

CD4 Monoclonal Antibody (GK1.5), Super Bright™ 600, eBioscience™

FIGURE: 1 / 98

CD4 Antibody (63-0041-82) in Flow

Staining of mouse splenocytes with Anti-Human/Mouse CD45R (B220) FITC (Product # 11-0452-82) and 0.06 µg of Rat IgG2b K Isotype Control Super Bright 600 (Product # 63-4031-82) (left) or 0.06 µg of Anti-Mouse CD4 Super Bright 600 (right). Total viable cells were used for analysis.

CD4 Antibody in Immunocytochemistry (ICC/IF)

CD4 Antibody in Immunohistochemistry (Frozen) (IHC (F))

CD4 Antibody in Immunohistochemistry (IHC)

CD4 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

Product Image Gallery

Product Details

63-0041-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 5 publications 5 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

0.125 µg/test

View 80 publications 80 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Super Bright™ 600, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

GK1.5

Conjugate

Super Bright™ 600 Super Bright™ 600 Super Bright™ 600

Excitation/Emission Max

414/601 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2717017

Product Specific Information

Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.

Applications Reported: This GK1.5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This GK1.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 600 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 600 nm. We recommend using a 610/20 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 600 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

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Epinephrine Production in Th17 Cells and Experimental Autoimmune Encephalitis.

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Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

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Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

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Runx proteins mediate protective immunity against Leishmania donovani infection by promoting CD40 expression on dendritic cells.

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HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

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Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Effects of combined treatment with PD‑L1 Ig and CD40L mAb on immune tolerance in the CBA/J x DBA/2 mouse model.

Molecular medicine reports

Li G,Yang L,Li D,Zhang J,Du L,Xia L,Liu Y,Hu W

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

FUT1 deficiency elicits immune dysregulation and corneal opacity in steady state and under stress.

Cell death & disease

Kim KW,Ryu JS,Ko JH,Kim JY,Kim HJ,Lee HJ,Oh JH,Chung JH,Oh JY

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Fri Apr 24 00:00:00 EDT 2020

Elevated granulocytic myeloid-derived suppressor cells are closely related with elevation of Th17 cells in mice with experimental asthma.

International journal of biological sciences

Xue F,Yu M,Li L,Zhang W,Ma Y,Dong L,Shan W,Zheng Y,Wang T,Feng D,Lv J,Wang X

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Fri Nov 12 00:00:00 EST 2021

Comparative Analysis of Age-Related Changes in Lacrimal Glands and Meibomian Glands of a C57BL/6 Male Mouse Model.

International journal of molecular sciences

Yoon CH,Ryu JS,Hwang HS,Kim MK

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Thu Jun 11 00:00:00 EDT 2020

Truncated PPM1D Prevents Apoptosis in the Murine Thymus and Promotes Ionizing Radiation-Induced Lymphoma.

Cells

Martinikova AS,Burocziova M,Stoyanov M,Macurek L

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Thu Sep 10 00:00:00 EDT 2020

Artificial Mini Dendritic Cells Boost T Cell-Based Immunotherapy for Ovarian Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Cheng S,Xu C,Jin Y,Li Y,Zhong C,Ma J,Yang J,Zhang N,Li Y,Wang C,Yang Z,Wang Y

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Ovarian granulosa cell tumor characterization identifies FOXL2 as an immunotherapeutic target.

JCI insight

Pierini S,Tanyi JL,Simpkins F,George E,Uribe-Herranz M,Drapkin R,Burger R,Morgan MA,Facciabene A

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Thu Aug 20 00:00:00 EDT 2020

Tankyrase inhibition sensitizes melanoma to PD-1 immune checkpoint blockade in syngeneic mouse models.

Communications biology

Waaler J,Mygland L,Tveita A,Strand MF,Solberg NT,Olsen PA,Aizenshtadt A,Fauskanger M,Lund K,Brinch SA,Lycke M,Dybing E,Nygaard V,Bøe SL,Heintz KM,Hovig E,Hammarström C,Corthay A,Krauss S

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Fri Apr 24 00:00:00 EDT 2020

The Proteomic Landscape of Resting and Activated CD4+ T Cells Reveal Insights into Cell Differentiation and Function.

International journal of molecular sciences

Subbannayya Y,Haug M,Pinto SM,Mohanty V,Meås HZ,Flo TH,Prasad TSK,Kandasamy RK

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Tue Dec 29 00:00:00 EST 2020

ICOS agonism by JTX-2011 (vopratelimab) requires initial T cell priming and Fc cross-linking for optimal T cell activation and anti-tumor immunity in preclinical models.

PloS one

Hanson A,Elpek K,Duong E,Shallberg L,Fan M,Johnson C,Wallace M,Mabry GR,Sazinsky S,Pepper L,Shu CJ,Sathyanarayanan S,Zuerndorfer S,Simpson T,Gostissa M,Briskin M,Law D,Michaelson J,Harvey CJ

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Mon Nov 16 00:00:00 EST 2020

Tregitopes regulate the tolerogenic immune response and decrease the foetal death rate in abortion-prone mouse matings.

Scientific reports

Kedzierska AE,Lorek D,Slawek A,Chelmonska-Soyta A

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Mon Jun 29 00:00:00 EDT 2020

Bixin Attenuates Experimental Autoimmune Encephalomyelitis by Suppressing TXNIP/NLRP3 Inflammasome Activity and Activating NRF2 Signaling.

Frontiers in immunology

Yu Y,Wu DM,Li J,Deng SH,Liu T,Zhang T,He M,Zhao YY,Xu Y

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Mon Jun 21 00:00:00 EDT 2021

Long-term ex vivo haematopoietic-stem-cell expansion allows nonconditioned transplantation.

Nature

Wilkinson AC,Ishida R,Kikuchi M,Sudo K,Morita M,Crisostomo RV,Yamamoto R,Loh KM,Nakamura Y,Watanabe M,Nakauchi H,Yamazaki S

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Mon Jul 01 00:00:00 EDT 2019

Expansion of Bacteriophages Is Linked to Aggravated Intestinal Inflammation and Colitis.

Cell host & microbe

Gogokhia L,Buhrke K,Bell R,Hoffman B,Brown DG,Hanke-Gogokhia C,Ajami NJ,Wong MC,Ghazaryan A,Valentine JF,Porter N,Martens E,O'Connell R,Jacob V,Scherl E,Crawford C,Stephens WZ,Casjens SR,Longman RS,Round JL

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Wed Feb 13 00:00:00 EST 2019

Characterization of immune cell subtypes in three commonly used mouse strains reveals gender and strain-specific variations.

Laboratory investigation; a journal of technical methods and pathology

Hensel JA,Khattar V,Ashton R,Ponnazhagan S

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

Bryostatin-1 ameliorated experimental colitis in Il-10^-/- Mice by protecting the intestinal barrier and limiting immune dysfunction.

Journal of cellular and molecular medicine

Zuo L,Li J,Ge S,Ge Y,Shen M,Wang Y,Zhou C,Wu R,Hu J

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2019

IL-31 plays dual roles in lung inflammation in an OVA-induced murine asthma model.

Biology open

Huang J,Yue H,Jiang T,Gao J,Shi Y,Shi B,Wu X,Gou X

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Tue Jan 15 00:00:00 EST 2019

Antitumor and immunomodulatory effects of a novel multitarget inhibitor, CS2164, in mouse hepatocellular carcinoma models.

Anti-cancer drugs

Zhou Y,Fu C,Kong Y,Pan D,Wang Y,Huang S,Li Z,Ning Z,Lu X,Shan S,Xin L

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2019

An attenuated Zika virus NS4B protein mutant is a potent inducer of antiviral immune responses.

NPJ vaccines

Li G,Adam A,Luo H,Shan C,Cao Z,Fontes-Garfias CR,Sarathy VV,Teleki C,Winkelmann ER,Liang Y,Sun J,Bourne N,Barrett ADT,Shi PY,Wang T

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Sex Differences in Mouse Popliteal Lymph Nodes.

Scientific reports

Dill-Garlow R,Chen KE,Walker AM

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Wed Jan 30 00:00:00 EST 2019

TLR1/2 Specific Small-Molecule Agonist Suppresses Leukemia Cancer Cell Growth by Stimulating Cytotoxic T Lymphocytes.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Cen X,Zhu G,Yang J,Yang J,Guo J,Jin J,Nandakumar KS,Yang W,Yin H,Liu S,Cheng K

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Fri May 17 00:00:00 EDT 2019

Molecular Profiling and Functional Analysis of Macrophage-Derived Tumor Extracellular Vesicles.

Cell reports

Cianciaruso C,Beltraminelli T,Duval F,Nassiri S,Hamelin R,Mozes A,Gallart-Ayala H,Ceada Torres G,Torchia B,Ries CH,Ivanisevic J,De Palma M

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Tue Jun 04 00:00:00 EDT 2019

MicroRNA-126 deficiency enhanced the activation and function of CD4⁺ T cells by elevating IRS-1 pathway.

Clinical and experimental immunology

Chu F,Hu Y,Zhou Y,Guo M,Lu J,Zheng W,Xu H,Zhao J,Xu L

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Thu Feb 01 00:00:00 EST 2018

Impact of hyperglycemia on autoimmune pancreatitis and regulatory T-cells.

World journal of gastroenterology

Müller-Graff FT,Fitzner B,Jaster R,Vollmar B,Zechner D

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Sat Jul 28 00:00:00 EDT 2018

Pyruvate dehydrogenase complex stimulation promotes immunometabolic homeostasis and sepsis survival.

JCI insight

McCall CE,Zabalawi M,Liu T,Martin A,Long DL,Buechler NL,Arts RJW,Netea M,Yoza BK,Stacpoole PW,Vachharajani V

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Thu Aug 09 00:00:00 EDT 2018

Photosensitizer Micelles Together with IDO Inhibitor Enhance Cancer Photothermal Therapy and Immunotherapy.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Peng J,Xiao Y,Li W,Yang Q,Tan L,Jia Y,Qu Y,Qian Z

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Tue May 01 00:00:00 EDT 2018

Microglia prevent peripheral immune cell invasion and promote an anti-inflammatory environment in the brain of APP-PS1 transgenic mice.

Journal of neuroinflammation

Unger MS,Schernthaner P,Marschallinger J,Mrowetz H,Aigner L

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Fri Sep 21 00:00:00 EDT 2018

Apigenin suppresses PD-L1 expression in melanoma and host dendritic cells to elicit synergistic therapeutic effects.

Journal of experimental & clinical cancer research : CR

Xu L,Zhang Y,Tian K,Chen X,Zhang R,Mu X,Wu Y,Wang D,Wang S,Liu F,Wang T,Zhang J,Liu S,Zhang Y,Tu C,Liu H

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Mon Oct 29 00:00:00 EDT 2018

Engineered erythrocytes covalently linked to antigenic peptides can protect against autoimmune disease.

Proceedings of the National Academy of Sciences of the United States of America

Pishesha N,Bilate AM,Wibowo MC,Huang NJ,Li Z,Deshycka R,Bousbaine D,Li H,Patterson HC,Dougan SK,Maruyama T,Lodish HF,Ploegh HL

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Tue Mar 21 00:00:00 EDT 2017

Distinct in vitro properties of embryonic and extraembryonic fibroblast-like cells are reflected in their in vivo behavior following grafting in the adult mouse brain.

Cell transplantation

Costa R,Bergwerf I,Santermans E,De Vocht N,Praet J,Daans J,Le Blon D,Hoornaert C,Reekmans K,Hens N,Goossens H,Berneman Z,Parolini O,Alviano F,Ponsaerts P

Published figure using CD4 monoclonal antibody (Product # 63-0041-82) in Flow Cytometry

Mon Nov 16 00:00:00 EST 2015

Bioinformatics

Protein Aliases: CD4; CD4 antigen p55; cd4a; fCD4; Leu-3; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

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Gene Aliases: Cd4; L3T4; Ly-4

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UniProt ID: (Mouse) P06332

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Entrez Gene ID: (Mouse) 12504

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Function(s)

glycoprotein binding protein binding zinc ion binding coreceptor activity enzyme binding protein kinase binding protein homodimerization activity

Process(es)

cytokine production immune system process induction by virus of host cell-cell fusion immune response cell adhesion cell surface receptor signaling pathway T cell differentiation maintenance of protein location in cell T cell activation T cell selection protein palmitoleylation positive regulation of protein kinase activity positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling regulation of T cell activation positive regulation of T cell activation

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CD4 Monoclonal Antibody (GK1.5), Super Bright™ 600, eBioscience™

CD4 Antibody (63-0041-82) in Flow

Product Details

63-0041-82

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