Invitrogen

CD4 Monoclonal Antibody (RM4-5), Super Bright™ 702, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

63 Published Figures

71 References

View all (605) CD4 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

FIGURE: 1 / 65

CD4 Antibody (67-0042-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD8 FITC (Product # 11-0081-82) and Rat IgG2a K Isotype Control (Product # 67-4321-82) (left) or 0.125 µg of Anti-Mouse CD4 Super Bright 702 (right). Cells in the lymphocyte gate were used for analysis.

CD4 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

CD4 Antibody in Immunocytochemistry (ICC/IF)

CD4 Antibody in Immunocytochemistry, Immunohistochemistry (PFA fixed) (ICC/IF, IHC (PFA))

CD4 Antibody in Immunohistochemistry (Frozen) (IHC (F))

CD4 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD4 Antibody — Staining of mouse splenocytes and bone marrow cells. Right: As expected based on known relative expression patterns, CD4 clone RM4-5 stains a subset of splenocytes and does not stain any bone marrow cells. Details: Balb/c bone marrow cells (left) and splenocytes (middle) were surface stained with CD4 (clone RM4-5) followed by staining with 7-AAD. Viable bone marrow cells in the lymphoid (blue histogram) and myeloid (purple histogram) gates and viable splenocytes (orange histogram) were used for analysis. {RE}

Product Details

67-0042-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 8 publications 8 publications

Immunohistochemistry (PFA fixed) (IHC (PFA))

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 3 publications 3 publications

Flow Cytometry (Flow)

0.125 µg/test

View 58 publications 58 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 702, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RM4-5

Conjugate

Super Bright™ 702 Super Bright™ 702 Super Bright™ 702

Excitation/Emission Max

413/702 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2662399

Product Specific Information

Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Tested: The RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 702 is a tandem dye that can be excited with the violet laser line (405 nm) and emits at 702 nm. We recommend using a 710/50 bandpass filter. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Staining Buffer (Product # SB-4401-42) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Swarna Bhasma Induces Antigen-Presenting Abilities of Macrophages and Helps Antigen Experienced CD4⁺ T Cells to Acquire Th1 Phenotypes Against Leishmania donovani Antigens.

Biological trace element research

Saini S,Anand A,Singh A,Mahapatra B,Sirohi S,Singh S,Singh RK

67-0042-82 was used in Flow cytometry/Cell sorting to suggest a definite potential of swarna bhasma in enhancing the effector functions of phagocytes and CD4+ T cells against L. donovani antigens.

Mon Jan 01 00:00:00 EST 2024

Impact of broad-spectrum antibiotics on the gut-microbiota-spleen-brain axis.

Brain, behavior, & immunity - health

Wan X,Eguchi A,Sakamoto A,Fujita Y,Yang Y,Qu Y,Hatano M,Mori C,Hashimoto K

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2023

Klebsiella pneumoniae Induces Inflammatory Bowel Disease Through Caspase-11-Mediated IL18 in the Gut Epithelial Cells.

Cellular and molecular gastroenterology and hepatology

Zhang Q,Su X,Zhang C,Chen W,Wang Y,Yang X,Liu D,Zhang Y,Yang R

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Thu Feb 23 00:00:00 EST 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Palmitoyl transferases act as novel drug targets for pancreatic cancer.

Journal of translational medicine

Lin Z,Lv Z,Liu X,Huang K

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Mon Apr 10 00:00:00 EDT 2023

SARS-CoV-2 spike protein promotes inflammatory cytokine activation and aggravates rheumatoid arthritis.

Cell communication and signaling : CCS

Lee AR,Woo JS,Lee SY,Lee YS,Jung J,Lee CR,Park SH,Cho ML

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Thu Mar 02 00:00:00 EST 2023

TRAF6 controls T cell homeostasis by maintaining the equilibrium of MALT1 scaffolding and protease functions.

Frontiers in immunology

O'Neill TJ,Gewies A,Seeholzer T,Krappmann D

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Complete Freund's Adjuvant Induces a Fibroblast-like Synoviocytes (FLS) Metabolic and Migratory Phenotype in Resident Fibroblasts of the Inoculated Footpad at the Earliest Stage of Adjuvant-Induced Arthritis.

Cells

González-Chávez SA,Chaparro-Barrera E,Alvarado-Jáquez MF,Cuevas-Martínez R,Ochoa-Albíztegui RE,Pacheco-Tena C

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Immunohistochemistry

Wed Mar 08 00:00:00 EST 2023

Aryl hydrocarbon receptor activation ameliorates experimental colitis by modulating the tolerogenic dendritic and regulatory T cell formation.

Cell & bioscience

Cui X,Ye Z,Wang D,Yang Y,Jiao C,Ma J,Tang N,Zhang H

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Sat Apr 23 00:00:00 EDT 2022

IL-33 induces thymic involution-associated naive T cell aging and impairs host control of severe infection.

Nature communications

Xu L,Wei C,Chen Y,Wu Y,Shou X,Chen W,Lu D,Sun H,Li W,Yu B,Wang X,Zhang X,Yu Y,Lei Z,Tang R,Zhu J,Li Y,Lu L,Zhou H,Zhou S,Su C,Chen X

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Sat Nov 12 00:00:00 EST 2022

Fringe GlcNAc-transferases differentially extend O-fucose on endogenous NOTCH1 in mouse activated T cells.

The Journal of biological chemistry

Matsumoto K,Kumar V,Varshney S,Nairn AV,Ito A,Pennarubia F,Moremen KW,Stanley P,Haltiwanger RS

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2022

Breast cancer cell-derived extracellular vesicles promote CD8⁺ T cell exhaustion via TGF-β type II receptor signaling.

Nature communications

Xie F,Zhou X,Su P,Li H,Tu Y,Du J,Pan C,Wei X,Zheng M,Jin K,Miao L,Wang C,Meng X,van Dam H,Ten Dijke P,Zhang L,Zhou F

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Black raspberry extract inhibits regulatory T-cell activity in a murine model of head and neck squamous cell carcinoma chemoprevention.

Frontiers in immunology

Ryan NM,Lamenza FF,Upadhaya P,Pracha H,Springer A,Swingler M,Siddiqui A,Oghumu S

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Immunohistochemistry

Mon Aug 29 00:00:00 EDT 2022

Niche-specific MHC II and PD-L1 regulate CD4+CD8αα+ intraepithelial lymphocyte differentiation.

The Journal of experimental medicine

Moon S,Park Y,Hyeon S,Kim YM,Kim JH,Kim H,Park S,Lee KJ,Koo BK,Ha SJ,Lee SW

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Mon Apr 05 00:00:00 EDT 2021

Niche-specific MHC II and PD-L1 regulate CD4+CD8αα+ intraepithelial lymphocyte differentiation.

The Journal of experimental medicine

Moon S,Park Y,Hyeon S,Kim YM,Kim JH,Kim H,Park S,Lee KJ,Koo BK,Ha SJ,Lee SW

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Mon Apr 05 00:00:00 EDT 2021

Romidepsin (FK228) regulates the expression of the immune checkpoint ligand PD-L1 and suppresses cellular immune functions in colon cancer.

Cancer immunology, immunotherapy : CII

Shi Y,Fu Y,Zhang X,Zhao G,Yao Y,Guo Y,Ma G,Bai S,Li H

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Potential therapeutic effects of interleukin-35 on the differentiation of naïve T cells into Helios⁺Foxp3⁺ Tregs in clinical and experimental acute respiratory distress syndrome.

Molecular immunology

Wang C,Xie K,Li K,Lin S,Xu F

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Feline adipose tissue-derived mesenchymal stem cells pretreated with IFN-γ enhance immunomodulatory effects through the PGE₂ pathway.

Journal of veterinary science

Park SG,An JH,Li Q,Chae HK,Park SM,Lee JH,Ahn JO,Song WJ,Youn HY

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Intratumoral expression of interleukin 23 variants using oncolytic vaccinia virus elicit potent antitumor effects on multiple tumor models via tumor microenvironment modulation.

Theranostics

Chen L,Chen H,Ye J,Ge Y,Wang H,Dai E,Ren J,Liu W,Ma C,Ju S,Guo ZS,Liu Z,Bartlett DL

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Wed Aug 04 00:00:00 EDT 2021

Tissue plasminogen activator worsens experimental autoimmune encephalomyelitis by complementary actions on lymphoid and myeloid cell responses.

Journal of neuroinflammation

Hélie P,Camacho-Toledano C,Lesec L,Seillier C,Miralles AJ,Ortega MC,Guérit S,Lebas H,Bardou I,Vila-Del Sol V,Vivien D,Le Mauff B,Clemente D,Docagne F,Toutirais O

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Immunohistochemistry

Sat Feb 20 00:00:00 EST 2021

Antitumor efficacy of a neoantigen cancer vaccine delivered by electroporation is influenced by microbiota composition.

Oncoimmunology

Lione L,Salvatori E,Petrazzuolo A,Massacci A,Maggio R,Confroti A,Compagnone M,Aurisicchio L,Ciliberto G,Palombo F

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Tue Mar 23 00:00:00 EDT 2021

Evaluation of DNA-Launched Virus-Like Particle Vaccines in an Immune Competent Mouse Model of Chikungunya Virus Infection.

Vaccines

Rayner JO,Kim JH,Roberts RW,Wood RR,Fouty B,Solodushko V

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Fri Apr 02 00:00:00 EDT 2021

Increased Levels of IL-16 in the Central Nervous System during Neuroinflammation Are Associated with Infiltrating Immune Cells and Resident Glial Cells.

Biology

Hridi SU,Barbour M,Wilson C,Franssen AJ,Harte T,Bushell TJ,Jiang HR

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Immunohistochemistry (Frozen)

Thu May 27 00:00:00 EDT 2021

Gut Microbiota Dysbiosis Induced by Intracerebral Hemorrhage Aggravates Neuroinflammation in Mice.

Frontiers in microbiology

Yu X,Zhou G,Shao B,Zhou H,Xu C,Yan F,Wang L,Chen G,Li J,Fu X

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Wed May 26 00:00:00 EDT 2021

Epithelial Mutant p53 Promotes Resistance to Anti-PD-1-Mediated Oral Cancer Immunoprevention in Carcinogen-Induced Mouse Models.

Cancers

Wang J,Hu Y,Escamilla-Rivera V,Gonzalez CL,Tang L,Wang B,El-Naggar AK,Myers JN,Caulin C

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Immunohistochemistry

Tue Mar 23 00:00:00 EDT 2021

Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials.

EBioMedicine

Okada R,Furusawa A,Vermeer DW,Inagaki F,Wakiyama H,Kato T,Nagaya T,Choyke PL,Spanos WC,Allen CT,Kobayashi H

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Activation of Bone Marrow Adaptive Immunity in Type 2 Diabetes: Rescue by Co-stimulation Modulator Abatacept.

Frontiers in immunology

Santopaolo M,Sullivan N,Thomas AC,Alvino VV,Nicholson LB,Gu Y,Spinetti G,Kallikourdis M,Blom A,Madeddu P

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Fri Jul 02 00:00:00 EDT 2021

Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer.

Nature communications

Huang Y,Guan Z,Dai X,Shen Y,Wei Q,Ren L,Jiang J,Xiao Z,Jiang Y,Liu D,Huang Z,Xu X,Luo Y,Zhao C

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Histological, behavioural and flow cytometric datasets relating to acute ischaemic stroke in young, aged and ApoE^-/- mice in the presence and absence of immunomodulation with fingolimod.

Data in brief

Diaz Diaz AC,Malone K,Shearer JA,Moore AC,Waeber C

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Ddb1 Is Essential for the Expansion of CD4⁺ Helper T Cells by Regulating Cell Cycle Progression and Cell Death.

Frontiers in immunology

Yang L,Chen W,Li L,Xiao Y,Fan S,Zhang Q,Xia T,Li M,Hong Y,Zhao T,Li Q,Liu WH,Xiao N

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Mon Dec 20 00:00:00 EST 2021

CD300a Receptor Blocking Enhances Early Clearance of Leishmania donovani From Its Mammalian Host Through Modulation of Effector Functions of Phagocytic and Antigen Experienced T Cells.

Frontiers in immunology

Singh R,Anand A,Rawat AK,Saini S,Mahapatra B,Singh NK,Mishra AK,Singh S,Singh N,Kishore D,Kumar V,Das P,Singh RK

67-0042-82 was used in Flow cytometry/Cell sorting to report that L. donovani, which causes visceral leishmaniasis in the Indian subcontinent, upregulates the expression of an immune inhibitory receptor i.e., CD300a on antigen presenting and phagocytic cells to dampen their effector functions.

Thu Feb 24 00:00:00 EST 2022

Local Sustained GM-CSF Delivery by Genetically Engineered Encapsulated Cells Enhanced Both Cellular and Humoral SARS-CoV-2 Spike-Specific Immune Response in an Experimental Murine Spike DNA Vaccination Model.

Vaccines

Vernet R,Charrier E,Cosset E,Fièvre S,Tomasello U,Grogg J,Mach N

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Mon May 10 00:00:00 EDT 2021

Sequencing, cloning, and antigen binding analysis of monoclonal antibodies isolated from single mouse B cells.

STAR protocols

Viant C,Escolano A,Chen ST,Nussenzweig MC

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Fri Jun 18 00:00:00 EDT 2021

Small-molecule antagonist of VLA-4 (GW559090) attenuated neuro-inflammation by targeting Th17 cell trafficking across the blood-retinal barrier in experimental autoimmune uveitis.

Journal of neuroinflammation

Chen YH,Eskandarpour M,Zhang X,Galatowicz G,Greenwood J,Lightman S,Calder V

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Thu Feb 18 00:00:00 EST 2021

Depletion of Retinal Dopaminergic Activity in a Mouse Model of Rod Dysfunction Exacerbates Experimental Autoimmune Uveoretinitis: A Role for the Gateway Reflex.

International journal of molecular sciences

Stofkova A,Zloh M,Andreanska D,Fiserova I,Kubovciak J,Hejda J,Kutilek P,Murakami M

67-0042-82 was used in Flow cytometry/Cell sorting to hypothesize that rod photoreceptor dysfunction causes remodeling of retinal neural activity, which influences the blood-retinal barrier and the development of retinal inflammation.

Fri Dec 31 00:00:00 EST 2021

Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry.

STAR protocols

Skordos I,Demeyer A,Beyaert R

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

STING activation normalizes the intraperitoneal vascular-immune microenvironment and suppresses peritoneal carcinomatosis of colon cancer.

Journal for immunotherapy of cancer

Lee SJ,Yang H,Kim WR,Lee YS,Lee WS,Kong SJ,Lee HJ,Kim JH,Cheon J,Kang B,Chon HJ,Kim C

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers.

PloS one

Cohen AA,Yang Z,Gnanapragasam PNP,Ou S,Dam KA,Wang H,Bjorkman PJ

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Fri Oct 15 00:00:00 EDT 2021

Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease.

Cell

Lee JY,Hall JA,Kroehling L,Wu L,Najar T,Nguyen HH,Lin WY,Yeung ST,Silva HM,Li D,Hine A,Loke P,Hudesman D,Martin JC,Kenigsberg E,Merad M,Khanna KM,Littman DR

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Immunocytochemistry

Thu Jan 09 00:00:00 EST 2020

Protein arginine methyltransferase 5 promotes cholesterol biosynthesis-mediated Th17 responses and autoimmunity.

The Journal of clinical investigation

Webb LM,Sengupta S,Edell C,Piedra-Quintero ZL,Amici SA,Miranda JN,Bevins M,Kennemer A,Laliotis G,Tsichlis PN,Guerau-de-Arellano M

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

DKK2 blockage-mediated immunotherapy enhances anti-angiogenic therapy of Kras mutated colorectal cancer.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Hu J,Wang Z,Chen Z,Li A,Sun J,Zheng M,Wu J,Shen T,Qiao J,Li L,Li B,Wu D,Xiao Q

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Comparison of rapamycin and methylprednisolone for treating inflammatory muscle disease in a murine model of experimental autoimmune myositis.

Experimental and therapeutic medicine

Kang J,Feng D,Yang F,Tian X,Han W,Jia H

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Effects of CD4⁺ T lymphocytes from ovariectomized mice on bone marrow mesenchymal stem cell proliferation and osteogenic differentiation.

Experimental and therapeutic medicine

Shao BY,Wang L,Yu Y,Chen L,Gan N,Huang WM

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Dietary Glucose Consumption Promotes RALDH Activity in Small Intestinal CD103⁺CD11b⁺ Dendritic Cells.

Frontiers in immunology

Ko HJ,Hong SW,Verma R,Jung J,Lee M,Kim N,Kim D,Surh CD,Kim KS,Rudra D,Im SH

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Mon Apr 19 00:00:00 EDT 2021

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Oncolytic vaccinia virus delivering tethered IL-12 enhances antitumor effects with improved safety.

Journal for immunotherapy of cancer

Ge Y,Wang H,Ren J,Liu W,Chen L,Chen H,Ye J,Dai E,Ma C,Ju S,Guo ZS,Liu Z,Bartlett DL

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

A novel antibody targeting TIM-3 resulting in receptor internalization for cancer immunotherapy.

Antibody therapeutics

Kuang Z,Li L,Zhang P,Chen B,Wu M,Ni H,Yi S,Zou J,Liu J

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Angioimmunoblastic T-cell lymphoma-like lymphadenopathy in mice transgenic for human RHOA with p.Gly17Val mutation.

Oncoimmunology

Lee GJ,Jun Y,Yoo HY,Jeon YK,Lee D,Lee S,Kim J

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Immunohistochemistry

Wed May 13 00:00:00 EDT 2020

Mechanisms of interleukin 4 mediated increase in efficacy of vaccines against opioid use disorders.

NPJ vaccines

Crouse B,Robinson C,Huseby Kelcher A,Laudenbach M,Abrahante JE,Pravetoni M

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Thu Oct 29 00:00:00 EDT 2020

Distinct actions of the fermented beverage kefir on host behaviour, immunity and microbiome gut-brain modules in the mouse.

Microbiome

van de Wouw M,Walsh AM,Crispie F,van Leuven L,Lyte JM,Boehme M,Clarke G,Dinan TG,Cotter PD,Cryan JF

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Mon May 18 00:00:00 EDT 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Thu Aug 27 00:00:00 EDT 2020

Loss of testosterone impairs anti-tumor neutrophil function.

Nature communications

Markman JL,Porritt RA,Wakita D,Lane ME,Martinon D,Noval Rivas M,Luu M,Posadas EM,Crother TR,Arditi M

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Tue Mar 31 00:00:00 EDT 2020

Positive Effects of Preventive Nutrition Supplement on Anticancer Radiotherapy in Lung Cancer Bearing Mice.

Cancers

Liu YM,Wu TH,Chiu YH,Wang H,Li TL,Hsia S,Chan YL,Wu CJ

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Immunohistochemistry

Fri Aug 28 00:00:00 EDT 2020

Prodrug-Loaded Zirconium Carbide Nanosheets as a Novel Biophotonic Nanoplatform for Effective Treatment of Cancer.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Liu Q,Xie Z,Qiu M,Shim I,Yang Y,Xie S,Yang Q,Wang D,Chen S,Fan T,Ding B,Guo Z,Adah D,Yao X,Zhang Y,Wu H,Wu Z,Wei C,Wang H,Kim HS,Zou Q,Yan Q,Cai Z,Kim JS,Liu LP,Zhang H,Cao Y

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Immunohistochemistry

Tue Dec 01 00:00:00 EST 2020

STING couples with PI3K to regulate actin reorganization during BCR activation.

Science advances

Jing Y,Dai X,Yang L,Kang D,Jiang P,Li N,Cheng J,Li J,Miller H,Ren B,Gong Q,Yin W,Liu Z,Mattila PK,Ning Q,Sun J,Yu B,Liu C

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Immunohistochemistry

Wed Apr 01 00:00:00 EDT 2020

A Commercial Probiotic Induces Tolerogenic and Reduces Pathogenic Responses in Experimental Autoimmune Encephalomyelitis.

Cells

Calvo-Barreiro L,Eixarch H,Ponce-Alonso M,Castillo M,Lebrón-Galán R,Mestre L,Guaza C,Clemente D,Del Campo R,Montalban X,Espejo C

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Tue Apr 07 00:00:00 EDT 2020

Axenic Caenorhabditis elegans antigen protects against development of type-1 diabetes in NOD mice.

Journal of translational autoimmunity

Jackson-Thompson BM,Torrero M,Mitre BK,Long J,Packiam M,Mitre E

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

T Cell Recruitment to the Intestinal Stem Cell Compartment Drives Immune-Mediated Intestinal Damage after Allogeneic Transplantation.

Immunity

Fu YY,Egorova A,Sobieski C,Kuttiyara J,Calafiore M,Takashima S,Clevers H,Hanash AM

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Immunocytochemistry

Tue Jul 16 00:00:00 EDT 2019

T Cell Recruitment to the Intestinal Stem Cell Compartment Drives Immune-Mediated Intestinal Damage after Allogeneic Transplantation.

Immunity

Fu YY,Egorova A,Sobieski C,Kuttiyara J,Calafiore M,Takashima S,Clevers H,Hanash AM

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Immunocytochemistry

Tue Jul 16 00:00:00 EDT 2019

Chloroquine modulates inflammatory autoimmune responses through Nurr1 in autoimmune diseases.

Scientific reports

Park TY,Jang Y,Kim W,Shin J,Toh HT,Kim CH,Yoon HS,Leblanc P,Kim KS

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Tue Oct 29 00:00:00 EDT 2019

Innate Sensing through Mesenchymal TLR4/MyD88 Signals Promotes Spontaneous Intestinal Tumorigenesis.

Cell reports

Koliaraki V,Chalkidi N,Henriques A,Tzaferis C,Polykratis A,Waisman A,Muller W,Hackam DJ,Pasparakis M,Kollias G

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Tue Jan 15 00:00:00 EST 2019

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Ziegler PK,Bollrath J,Pallangyo CK,Matsutani T,Canli Ö,De Oliveira T,Diamanti MA,Müller N,Gamrekelashvili J,Putoczki T,Horst D,Mankan AK,Öner MG,Müller S,Müller-Höcker J,Kirchner T,Slotta-Huspenina J,Taketo MM,Reinheckel T,Dröse S,Larner AC,Wels WS,Ernst M,Greten TF,Arkan MC,Korn T,Wirth D,Greten FR

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Thu Jun 28 00:00:00 EDT 2018

TCF-1 and HEB cooperate to establish the epigenetic and transcription profiles of CD4⁺CD8⁺ thymocytes.

Nature immunology

Emmanuel AO,Arnovitz S,Haghi L,Mathur PS,Mondal S,Quandt J,Okoreeh MK,Maienschein-Cline M,Khazaie K,Dose M,Gounari F

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5.

The Journal of experimental medicine

Buxadé M,Huerga Encabo H,Riera-Borrull M,Quintana-Gallardo L,López-Cotarelo P,Tellechea M,Martínez-Martínez S,Redondo JM,Martín-Caballero J,Flores JM,Bosch E,Rodríguez-Fernández JL,Aramburu J,López-Rodríguez C

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Mon Nov 05 00:00:00 EST 2018

Runx-dependent and silencer-independent repression of a maturation enhancer in the Cd4 gene.

Nature communications

Kojo S,Yasmin N,Muroi S,Tenno M,Taniuchi I

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Wed Sep 05 00:00:00 EDT 2018

IL-36 Signaling Facilitates Activation of the NLRP3 Inflammasome and IL-23/IL-17 Axis in Renal Inflammation and Fibrosis.

Journal of the American Society of Nephrology : JASN

Chi HH,Hua KF,Lin YC,Chu CL,Hsieh CY,Hsu YJ,Ka SM,Tsai YL,Liu FC,Chen A

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2017

Enhanced T-cell activation and differentiation in lymphocytes from transgenic mice expressing ubiquitination-resistant 2KR LAT molecules.

Gene therapy

Rodriguez-Peña AB,Gomez-Rodriguez J,Kortum RL,Palmer DC,Yu Z,Guittard GC,Wohlfert EA,Silver PB,Misplon JA,Sommers CL,Feigenbaum L,Epstein SL,Caspi RR,Belkaid Y,Restifo NP,Samelson LE,Balagopalan L

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation.

eLife

He L,Zhang Y,Ma G,Tan P,Li Z,Zang S,Wu X,Jing J,Fang S,Zhou L,Wang Y,Huang Y,Hogan PG,Han G,Zhou Y

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2015

P2X7 receptor modulates inflammatory and functional pulmonary changes induced by silica.

PloS one

Monção-Ribeiro LC,Faffe DS,Santana PT,Vieira FS,da Graça CL,Marques-da-Silva C,Machado MN,Caruso-Neves C,Zin WA,Borojevic R,Takiya CM,Coutinho-Silva R

Published figure using CD4 monoclonal antibody (Product # 67-0042-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2015

Bioinformatics

Protein Aliases: CD4; CD4 antigen p55; cd4a; fCD4; Leu-3; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

View more View less

Gene Aliases: Cd4; L3T4; Ly-4

View more View less

UniProt ID: (Mouse) P06332

View more View less

Entrez Gene ID: (Mouse) 12504

View more View less

Function(s)

glycoprotein binding protein binding zinc ion binding coreceptor activity enzyme binding protein kinase binding protein homodimerization activity

Process(es)

cytokine production immune system process induction by virus of host cell-cell fusion immune response cell adhesion cell surface receptor signaling pathway T cell differentiation maintenance of protein location in cell T cell activation T cell selection protein palmitoleylation positive regulation of protein kinase activity positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling regulation of T cell activation positive regulation of T cell activation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD4 Monoclonal Antibody (RM4-5), Super Bright™ 702, eBioscience™

CD4 Antibody (67-0042-82) in Flow

Product Details

67-0042-82

Immunohistochemistry (IHC)

Immunohistochemistry (PFA fixed) (IHC (PFA))

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!