Invitrogen

Arginase 1 Monoclonal Antibody (A1exF5), PE, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

14 Published Figures

18 References

View all (94) Arginase 1 antibodies

Datasheet

Protocols

Questions & Answers

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Arginase 1 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 16

Arginase 1 Antibody (12-3697-82) in Flow

Normal human lysed whole blood cells were stained intracellularly, using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol, with 0.25 µg of Arginase 1 Monoclonal Antibody, PE. Cells were co-stained with CD16 Monoclonal Antibody, FITC (Product # 11-0168-42) and CD193 Monoclonal Antibody, APC (Product # 17-1939-42). Cells in the granulocyte gate (left panel) were used for analysis and were gated on either the CD193+ population (blue histogram) or the CD16+ population (purple histogram).

Arginase 1 Antibody in Immunohistochemistry (Frozen) (IHC (F))

Product Image Gallery

Product Details

12-3697-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 17 publications 17 publications

Product Specifications

Species Reactivity

Human, Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

A1exF5

Immunogen

E.coli-derived recombinant mouse Arginase 1

Conjugate

PE PE PE

Excitation/Emission Max

565/576 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2734839

Product Specific Information

Description: The monoclonal antibody A1exF5 recognizes both human and mouse Arginase 1, a cytosolic enzyme (Arg1). This A1exF5 clone is compatible with both the standard intracellular protocols and the Foxp3/Transcription Factor Staining Buffer Set.

Applications Reported: This A1exF5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This A1exF5 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells using the Intracellular Fixation & Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

Arginase-1 (Arg1) is a 35 kDa enzyme converting L-arginine to urea and L-ornithine, which is the final step in the urea cycle. The resulting polyamines are important for cell proliferation and removal of toxins that arise from protein degradation. By degrading arginine, Arginase 1 deprives NO synthase of its substrate and down-regulates nitric oxide production. In both human and mouse, Arginase 1 is expressed in the liver, neutrophils, myeloid derived suppressor cells (MDSC) and neural stem cells. In human, expression in blood neutrophils but not in CCR3+ granulocytes has been reported. In mice, expression of Arginase 1 is one of the hallmarks of alternatively activated macrophages (M2a). Arginase-1 may be expressed in the myeloid cells infiltrating tumors, and is typically found in the majority of hepatocellular carcinomas. Defects in Arginase 1 are the cause of argininemia, an autosomal recessive disorder characterized by hyperammonemia.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Advanced phosphocreatine-grafted chitosan hydrogel promote wound healing by macrophage modulation.

Frontiers in bioengineering and biotechnology

Sheng W,Qin H,Wang T,Zhao J,Fang C,Zhang P,Liu P,Udduttula A,Zeng H,Chen Y

Published figure using Arginase 1 monoclonal antibody (Product # 12-3697-82) in Flow Cytometry

Wed May 31 00:00:00 EDT 2023

Timing of Interleukin-4 Stimulation of Macrophages Determines Their Anti-Microbial Activity during Infection with Salmonella enterica Serovar Typhimurium.

Cells

Brigo N,Neumaier E,Pfeifhofer-Obermair C,Grubwieser P,Engl S,Berger S,Seifert M,Reinstadler V,Oberacher H,Weiss G

Published figure using Arginase 1 monoclonal antibody (Product # 12-3697-82) in Flow Cytometry

Fri Apr 14 00:00:00 EDT 2023

Repurposing nitric oxide donating drugs in cancer therapy through immune modulation.

Journal of experimental & clinical cancer research : CR

Li CY,Anuraga G,Chang CP,Weng TY,Hsu HP,Ta HDK,Su PF,Chiu PH,Yang SJ,Chen FW,Ye PH,Wang CY,Lai MD

Published figure using Arginase 1 monoclonal antibody (Product # 12-3697-82) in Flow Cytometry

Sat Jan 14 00:00:00 EST 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

Published figure using Arginase 1 monoclonal antibody (Product # 12-3697-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis.

Veterinary research

Feng L,Luo G,Li Y,Zhang C,Liu Y,Liu Y,Chen H,He D,Zhu Y,Gan L

12-3697-82 was used in Flow cytometry/Cell sorting to demonstrate that CUR protected against PRV-induced viral encephalitis by switching the phenotype of BV2 cells, thereby protecting neurons from inflammatory injury, and this effect was mediated by improving mitochondrial function and the AMPK/NF-κB p65-energy metabolism-related pathway.

Tue Mar 14 00:00:00 EDT 2023

Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis.

Veterinary research

Feng L,Luo G,Li Y,Zhang C,Liu Y,Liu Y,Chen H,He D,Zhu Y,Gan L

Tue Mar 14 00:00:00 EDT 2023

Interleukin-10 genetically modified clinical-grade mesenchymal stromal cells markedly reinforced functional recovery after spinal cord injury via directing alternative activation of macrophages.

Cellular & molecular biology letters

Gao T,Huang F,Wang W,Xie Y,Wang B

Published figure using Arginase 1 monoclonal antibody (Product # 12-3697-82) in Flow Cytometry

Thu Mar 17 00:00:00 EDT 2022

Angiotensin 1-7 and its analogue decrease blood pressure but aggravate renal damage in preeclamptic mice.

Experimental animals

Liu Y,Zhai R,Tong J,Yu Y,Yang L,Gu Y,Niu J

Published figure using Arginase 1 monoclonal antibody (Product # 12-3697-82) in Flow Cytometry

Thu Nov 10 00:00:00 EST 2022

LINC00240/miR-155 axis regulates function of trophoblasts and M2 macrophage polarization via modulating oxidative stress-induced pyroptosis in preeclampsia.

Molecular medicine (Cambridge, Mass.)

Wu HY,Liu K,Zhang JL

Published figure using Arginase 1 monoclonal antibody (Product # 12-3697-82) in Flow Cytometry

Sat Sep 24 00:00:00 EDT 2022

Gene-selective transcription promotes the inhibition of tissue reparative macrophages by TNF.

Life science alliance

Dichtl S,Sanin DE,Koss CK,Willenborg S,Petzold A,Tanzer MC,Dahl A,Kabat AM,Lindenthal L,Zeitler L,Satzinger S,Strasser A,Mann M,Roers A,Eming SA,El Kasmi KC,Pearce EJ,Murray PJ

Published figure using Arginase 1 monoclonal antibody (Product # 12-3697-82) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2022

Hybrid Biomaterial Initiates Refractory Wound Healing via Inducing Transiently Heightened Inflammatory Responses.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Liu X,Dou G,Li Z,Wang X,Jin R,Liu Y,Kuang H,Huang X,Yang X,Yang X,Liu S,Wu M,Guo H,Ding F,Xu H,Liu S,Jin Y,Xuan K

12-3697-82 was used in Flow cytometry/Cell sorting to investigate hybrid biomaterial use for wound healing and associated inflammation.

Fri Jul 01 00:00:00 EDT 2022

Repolarization of Tumor-Infiltrating Myeloid Cells for Augmentation of CAR T Cell Therapies.

Frontiers in immunology

Luo W,Napoleon JV,Zhang F,Lee YG,Wang B,Putt KS,Low PS

12-3697-82 was used in Flow cytometry/Cell sorting to show that co-administration of the FA-TLR7-1A with the CAR T cell therapy not only repolarizes TAMs and MDSCs from an M2-like anti-inflammatory to M1-like pro-inflammatory phenotype, but also enhances both CAR T cell and endogenous T cell accumulation in solid tumors while concurrently increasing their states of activation.

Mon May 02 00:00:00 EDT 2022

Cutting Edge: Enhanced Antitumor Immunity in ST8Sia6 Knockout Mice.

Journal of immunology (Baltimore, Md. : 1950)

Friedman DJ,Kizerwetter M,Belmonte P,Rajcula M,Theodore K,Kim Lee HS,Shapiro MJ,Dong H,Shapiro VS

12-3697-82 was used in Flow cytometry/Cell sorting to examine the role of ST8Sia6 on immune cells in regulating antitumor immunity. ST8Sia6 knockout mice had an enhanced immune response to tumors.

Fri Apr 15 00:00:00 EDT 2022

Lactate and IL6 define separable paths of inflammatory metabolic adaptation.

Science advances

Dichtl S,Lindenthal L,Zeitler L,Behnke K,Schlösser D,Strobl B,Scheller J,El Kasmi KC,Murray PJ

Published figure using Arginase 1 monoclonal antibody (Product # 12-3697-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Alphavirus-Driven Interferon Gamma (IFNg) Expression Inhibits Tumor Growth in Orthotopic 4T1 Breast Cancer Model.

Vaccines

Trofimova O,Korotkaja K,Skrastina D,Jansons J,Spunde K,Isaguliants M,Zajakina A

Published figure using Arginase 1 monoclonal antibody (Product # 12-3697-82) in Flow Cytometry

Wed Oct 27 00:00:00 EDT 2021

Regulatory T Cells License Macrophage Pro-Resolving Functions During Atherosclerosis Regression.

Circulation research

Sharma M,Schlegel MP,Afonso MS,Brown EJ,Rahman K,Weinstock A,Sansbury BE,Corr EM,van Solingen C,Koelwyn GJ,Shanley LC,Beckett L,Peled D,Lafaille JJ,Spite M,Loke P,Fisher EA,Moore KJ

Published figure using Arginase 1 monoclonal antibody (Product # 12-3697-82) in Immunohistochemistry (Frozen)

Fri Jul 17 00:00:00 EDT 2020

Chinese Medicine Formula PSORI-CM02 Alleviates Psoriatic Dermatitis via M-MDSCs and Th17 Crosstalk.

Frontiers in pharmacology

Deng J,Tan S,Liu R,Yu W,Chen H,Tang N,Han L,Lu C

Published figure using Arginase 1 monoclonal antibody (Product # 12-3697-82) in Flow Cytometry

Sat Feb 06 00:00:00 EST 2021

Metformin as a senostatic drug enhances the anticancer efficacy of CDK4/6 inhibitor in head and neck squamous cell carcinoma.

Cell death & disease

Hu Q,Peng J,Jiang L,Li W,Su Q,Zhang J,Li H,Song M,Cheng B,Xia J,Wu T

Published figure using Arginase 1 monoclonal antibody (Product # 12-3697-82) in Flow Cytometry

Wed Oct 28 00:00:00 EDT 2020

Regulation of the tumor immune microenvironment and vascular normalization in TNBC murine models by a novel peptide.

Oncoimmunology

Mirando AC,Patil A,Rafie CI,Christmas BJ,Pandey NB,Stearns V,Jaffee EM,Roussos Torres ET,Popel AS

Published figure using Arginase 1 monoclonal antibody (Product # 12-3697-82) in Flow Cytometry

Thu May 14 00:00:00 EDT 2020

Bioinformatics

Protein Aliases: A-I; arginase 1 liver; arginase 1, liver; arginase I; arginase, liver; Arginase-1; Arginase1; HGNC:663; Liver Arginase; Liver-type arginase; Type 1 Arginase; Type I arginase

View more View less

Gene Aliases: AI; AI256583; Arg-1; ARG1; PGIF

View more View less

UniProt ID: (Human) P05089, (Mouse) Q61176

View more View less

Entrez Gene ID: (Human) 383, (Mouse) 11846

View more View less

Function(s)

arginase activity manganese ion binding hydrolase activity hydrolase activity, acting on carbon-nitrogen (but not peptide) bonds, in linear amidines metal ion binding hydrolase

Process(es)

urea cycle liver development positive regulation of endothelial cell proliferation arginine catabolic process aging response to herbicide response to manganese ion response to zinc ion response to selenium ion regulation of L-arginine import response to amine arginine catabolic process to ornithine lung development collagen biosynthetic process response to vitamin A response to vitamin E response to drug response to amino acid response to cadmium ion response to axon injury response to methylmercury mammary gland involution maternal process involved in female pregnancy protein homotrimerization cellular response to hydrogen peroxide cellular response to lipopolysaccharide cellular response to interleukin-4 cellular response to glucagon stimulus cellular response to dexamethasone stimulus cellular response to transforming growth factor beta stimulus arginine metabolic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

Arginase 1 Monoclonal Antibody (A1exF5), PE, eBioscience™

Arginase 1 Antibody (12-3697-82) in Flow

Product Details

12-3697-82

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!