Invitrogen

CD115 (c-fms) Monoclonal Antibody (AFS98), Super Bright™ 436, eBioscience™

19 Published Figures

19 References

View all (80) CSF1R (CD115) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (19)

CD115 (c-fms) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 20

CD115 (c-fms) Antibody (62-1152-82) in Flow

C57BL/6 mouse thioglycolate-elicited peritoneal exudate cells were stained with CD11b Monoclonal Antibody, APC (Product # 17-0112-82) and CD115 (c-fms) Monoclonal Antibody, Super Bright 645. Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

Product Details

62-1152-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 19 publications 19 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Super Bright™ 436, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AFS98

Conjugate

Super Bright™ 436 Super Bright™ 436 Super Bright™ 436

Excitation/Emission Max

413/431 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2688111

Product Specific Information

Description: The AFS98 monoclonal antibody reacts with the mouse CD115 molecule, a receptor for macrophage colony stimulating factor (M-CSF) or colony stimulating factor-1 (CSF-1). CD115 is expressed by monocyte, macrophage, osteoclast, and some epithelial cells. It is a 150 kDa c-fms gene product and belongs to immunoglobulin family. CSF-1 signaling through CSF-1R regulates the proliferation and differentiation of cells in the monocytic lineage.

Applications Reported: This AFS98 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AFS98 antibody has been tested by flow cytometric analysis of thioglycolate-elicited peritoneal macrophages. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.

When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.

Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser

Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.

Target Information

CSF1R (FMS) is involved in the production, differentiation, and function of macrophages, and is implicated in promyelocytic leukemias. Ligand binding activates the receptor kinase through a process of oligomerization and transphosphorylation. CSF1R is a tyrosine kinase transmembrane receptor and member of the CSF1/PDGF receptor family of tyrosine-protein kinases. Mutations in the gene encoding CSF1R have been associated with a predisposition to myeloid malignancy.Tyrosine-protein kinase that acts as cell-surface receptor for CSF1 and IL34 and plays an essential role in the regulation of survival, proliferation and differentiation of hematopoietic precursor cells, especially mononuclear phagocytes, such as macrophages and monocytes. Promotes the release of proinflammatory chemokines in response to IL34 and CSF1, and plays an important role in innate immunity and in inflammatory processes. The first intron of the CSF1R gene contains a transcriptionally inactive ribosomal protein L7 processed pseudogene oriented in the opposite direction. Mutations in the CSF1R gene have been associated with a predisposition to myeloid malignancy.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Pyruvate dehydrogenase kinase supports macrophage NLRP3 inflammasome activation during acute inflammation.

Cell reports

Meyers AK,Wang Z,Han W,Zhao Q,Zabalawi M,Duan L,Liu J,Zhang Q,Manne RK,Lorenzo F,Quinn MA,Song Q,Fan D,Lin HK,Furdui CM,Locasale JW,McCall CE,Zhu X

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Tue Jan 31 00:00:00 EST 2023

Platelets exacerbate cardiovascular inflammation in a murine model of Kawasaki disease vasculitis.

JCI insight

Kocatürk B,Lee Y,Nosaka N,Abe M,Martinon D,Lane ME,Moreira D,Chen S,Fishbein MC,Porritt RA,Franklin BS,Noval Rivas M,Arditi M

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Mon Jul 24 00:00:00 EDT 2023

Pexidartinib synergize PD-1 antibody through inhibiting treg infiltration by reducing TAM-derived CCL22 in lung adenocarcinoma.

Frontiers in pharmacology

Zhang W,Jiang X,Zou Y,Yuan L,Wang X

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Tue Mar 28 00:00:00 EDT 2023

MARCH3 negatively regulates IL-3-triggered inflammatory response by mediating K48-linked polyubiquitination and degradation of IL-3Rα.

Signal transduction and targeted therapy

Feng L,Li C,Zeng LW,Gao D,Sun YH,Zhong L,Lin H,Shu HB,Li S

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Mon Jan 24 00:00:00 EST 2022

Loss of phosphatidylinositol-4-phosphate 5-kinase type-1 gamma (Pip5k1c) in mesenchymal stem cells leads to osteopenia by impairing bone remodeling.

The Journal of biological chemistry

Yan Q,Gao H,Yao Q,Ling K,Xiao G

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Tue Mar 01 00:00:00 EST 2022

Mapping the origin and fate of myeloid cells in distinct compartments of the eye by single-cell profiling.

The EMBO journal

Wieghofer P,Hagemeyer N,Sankowski R,Schlecht A,Staszewski O,Amann L,Gruber M,Koch J,Hausmann A,Zhang P,Boneva S,Masuda T,Hilgendorf I,Goldmann T,Böttcher C,Priller J,Rossi FM,Lange C,Prinz M

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Mon Mar 15 00:00:00 EDT 2021

Low-dose 2-deoxy glucose stabilises tolerogenic dendritic cells and generates potent in vivo immunosuppressive effects.

Cellular and molecular life sciences : CMLS

Christofi M,Le Sommer S,Mölzer C,Klaska IP,Kuffova L,Forrester JV

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

HOXA11 plays critical roles in disease progression and response to cytarabine in AML.

Oncology reports

Fu JF,Shih LY,Yen TH

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Thu Jul 01 00:00:00 EDT 2021

Inhibition of TLR4 signaling protects mice from sensory and motor dysfunction in an animal model of autoimmune peripheral neuropathy.

Journal of neuroinflammation

Oladiran O,Shi XQ,Yang M,Fournier S,Zhang J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Mon Mar 22 00:00:00 EDT 2021

CX3CR1 But Not CCR2 Expression Is Required for the Development of Autoimmune Peripheral Neuropathy in Mice.

Frontiers in immunology

Oladiran O,Shi XQ,Fournier S,Zhang J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Tue Dec 21 00:00:00 EST 2021

Inflammation rapidly recruits mammalian GMP and MDP from bone marrow into regional lymphatics.

eLife

Serrano-Lopez J,Hegde S,Kumar S,Serrano J,Fang J,Wellendorf AM,Roche PA,Rangel Y,Carrington LJ,Geiger H,Grimes HL,Luther S,Maillard I,Sanchez-Garcia J,Starczynowski DT,Cancelas JA

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Thu Apr 08 00:00:00 EDT 2021

Hepatocyte-specific glucose-6-phosphatase deficiency disturbs platelet aggregation and decreases blood monocytes upon fasting-induced hypoglycemia.

Molecular metabolism

La Rose AM,Bazioti V,Hoogerland JA,Svendsen AF,Groenen AG,van Faassen M,Rutten MGS,Kloosterhuis NJ,Dethmers-Ausema B,Nijland JH,Mithieux G,Rajas F,Kuipers F,Lukens MV,Soehnlein O,Oosterveer MH,Westerterp M

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Mon Nov 01 00:00:00 EDT 2021

Radiation causes tissue damage by dysregulating inflammasome-gasdermin D signaling in both host and transplanted cells.

PLoS biology

Xiao J,Wang C,Yao JC,Alippe Y,Yang T,Kress D,Sun K,Kostecki KL,Monahan JB,Veis DJ,Abu-Amer Y,Link DC,Mbalaviele G

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

Temporospatial distribution and transcriptional profile of retinal microglia in the oxygen-induced retinopathy mouse model.

Glia

Boeck M,Thien A,Wolf J,Hagemeyer N,Laich Y,Yusuf D,Backofen R,Zhang P,Boneva S,Stahl A,Hilgendorf I,Agostini H,Prinz M,Wieghofer P,Schlunck G,Schlecht A,Lange C

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Androgen Receptor Signaling Positively Regulates Monocytic Development.

Frontiers in immunology

Consiglio CR,Gollnick SO

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Thu Apr 29 00:00:00 EDT 2021

Microglia drive APOE-dependent neurodegeneration in a tauopathy mouse model.

The Journal of experimental medicine

Shi Y,Manis M,Long J,Wang K,Sullivan PM,Remolina Serrano J,Hoyle R,Holtzman DM

62-1152 was used in Flow cytometry/Cell sorting to demonstrate that microglial activation is the driving force of neurodegeneration in the setting of tauopathy in a mouse model.

Mon Nov 04 00:00:00 EST 2019

Concomitant type I IFN and M-CSF signaling reprograms monocyte differentiation and drives pro-tumoral arginase production.

EBioMedicine

Tong Y,Zhou L,Yang L,Guo P,Cao Y,Qin FX,Liu J

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

Captopril mitigates splenomegaly and myelofibrosis in the Gata1^low murine model of myelofibrosis.

Journal of cellular and molecular medicine

Corey SJ,Jha J,McCart EA,Rittase WB,George J,Mattapallil JJ,Mehta H,Ognoon M,Bylicky MA,Summers TA,Day RM

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Neutrophil and Macrophage Cell Surface Colony-Stimulating Factor 1 Shed by ADAM17 Drives Mouse Macrophage Proliferation in Acute and Chronic Inflammation.

Molecular and cellular biology

Tang J,Frey JM,Wilson CL,Moncada-Pazos A,Levet C,Freeman M,Rosenfeld ME,Stanley ER,Raines EW,Bornfeldt KE

Published figure using CD115 (c-fms) monoclonal antibody (Product # 62-1152-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Bioinformatics

Protein Aliases: c-fms; CD115; CSF-1 receptor; CSF-1-R; CSFIR; EC 2.7.10.1; kinase CSFR; Macrophage colony-stimulating factor 1 receptor; OTTHUMP00000224093; Proto-oncogene c-Fms; proto-oncogene fms

View more View less

Gene Aliases: AI323359; CD115; CSF-1R; Csf1r; Csfmr; Fim-2; Fms; M-CSF-R; M-CSFR

View more View less

UniProt ID: (Mouse) P09581

View more View less

Entrez Gene ID: (Mouse) 12978

View more View less

Function(s)

nucleotide binding protein kinase activity protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity macrophage colony-stimulating factor receptor activity protein binding ATP binding kinase activity transferase activity transferase activity, transferring phosphorus-containing groups protein phosphatase binding cytokine binding protein homodimerization activity transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD115 (c-fms) Monoclonal Antibody (AFS98), Super Bright™ 436, eBioscience™

CD115 (c-fms) Antibody (62-1152-82) in Flow

Product Details

62-1152-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!