Invitrogen

CD278 (ICOS) Monoclonal Antibody (7E.17G9), PE-Cyanine7, eBioscience™

CD278 (ICOS) Antibody in Flow Cytometry (Flow)

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CD278 (ICOS) Antibody (25-9942-82) in Flow

Staining of unstimulated (left) or 2-day Con A-stimulated (right) mouse splenocytes with Anti-Mouse CD25 APC (Product # 17-0251-82) and 0.125 µg of Anti-Mouse CD278 (ICOS) PE-Cyanine7. Total viable cells were used for analysis. Quadrant lines were based on staining with 0.125 µg of Rat IgG2b K Isotype Control PE-Cyanine7 (Product # 25-4031-82).

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Product Details

25-9942-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 11 publications 11 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), PE-Cyanine7, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

7E.17G9

Conjugate

PE-Cyanine7 PE-Cyanine7 PE-Cyanine7

Excitation/Emission Max

569/780 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2573564

Product Specific Information

Description: The 7E.17G9 monoclonal antibody reacts with mouse ICOS (Inducible COStimulatory molecule), a T cell specific molecule and a third member of the CD28/CTLA-4 family. A homodimer of 47-57 kDa, ICOS is expressed on activated T cells, has potent costimulatory activity for T cell activation and proliferation and is required for humoral immune response. ICOS binds to its ligand on activated APC including B cells called B7h/B7RP-1 and is thought to play a protective role in inflammatory autoimmune diseases. ICOS may be involved in the development of Th2 cells.

Applications Reported: This 7E.17G9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 7E.17G9 antibody has been tested by flow cytometric analysis of stimulated mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 775 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

ICOS (CD278) encodes a protein that is a member of the CD28 and CTLA-4 cell-surface receptor family. It functions as a homodimer and has a crucial role in cell-cell signaling, immune responses, and the regulation of cell proliferation. ICOS has been linked to various disorders, including Immunodeficiency, Common Variable, 1 and Common Variable Immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9942-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9942-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Group 2 innate lymphoid cells protect mouse heart from myocardial infarction injury via interleukin 5, eosinophils, and dendritic cells.

Cardiovascular research

Liu T,Meng Z,Liu J,Li J,Zhang Y,Deng Z,Luo S,Wang M,Huang Q,Zhang S,Fendt P,Devouassoux J,Li D,McKenzie ANJ,Nahrendorf M,Libby P,Guo J,Shi GP

25-9942-82 was used in Flow cytometry/Cell sorting to reveal an indirect cardiac reparative role of ILC2 in post-MI hearts via the IL5, EOS, and DC mechanism.

Tue May 02 00:00:00 EDT 2023

Overcoming resistance to αPD-1 of MMR-deficient tumors with high tumor-induced neutrophils levels by combination of αCTLA-4 and αPD-1 blockers.

Journal for immunotherapy of cancer

Nebot-Bral L,Hollebecque A,Yurchenko AA,de Forceville L,Danjou M,Jouniaux JM,Rosa RCA,Pouvelle C,Aoufouchi S,Vuagnat P,Smolenschi C,Colomba E,Leary A,Marabelle A,Scoazec JY,Cassard L,Nikolaev S,Chaput N,Kannouche P

25-9942-82 was used in Flow cytometry/Cell sorting to reveal that early on-treatment change in the NLR ratio may predict resistance to αPD-1 therapy.

Fri Jul 01 00:00:00 EDT 2022

The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity.

Cellular & molecular immunology

Huang X,Hao S,Liu J,Huang Y,Liu M,Xiao C,Wang Y,Pei S,Yu T,Xu J,Wang H,Dai D,Su X,Xiao Y

25-9942-82 was used in Flow Cytometry to elucidate the molecular mechanisms regulating T follicular helper cells.

Thu Apr 01 00:00:00 EDT 2021

Tissue-restricted control of established central nervous system autoimmunity by TNF receptor 2-expressing Treg cells.

Proceedings of the National Academy of Sciences of the United States of America

Ronin E,Pouchy C,Khosravi M,Hilaire M,Grégoire S,Casrouge A,Kassem S,Sleurs D,Martin GH,Chanson N,Lombardi Y,Lalle G,Wajant H,Auffray C,Lucas B,Marodon G,Grinberg-Bleyer Y,Salomon BL

25-9942-82 was used in Flow cytometry/Cell sorting to show that endogenous Treg cells specifically suppress an autoimmune disease by acting in the target tissue during overt inflammation.

Tue Mar 30 00:00:00 EDT 2021

TCF1 and LEF1 Control Treg Competitive Survival and Tfr Development to Prevent Autoimmune Diseases.

Cell reports

Yang BH,Wang K,Wan S,Liang Y,Yuan X,Dong Y,Cho S,Xu W,Jepsen K,Feng GS,Lu LF,Xue HH,Fu W

25-9942-82 was used in Flow Cytometry to study how CD4+ Foxp3+ T regulatory (Treg) cell differentiation and functional specification is regulated.

Tue Jun 18 00:00:00 EDT 2019

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

25-9942 was used in Flow Cytometry to indicate that the VHL-HIF-glycolysis axis is essential for the late-stage maturation and function of ILC2s via targeting IL-33-ST2 pathway.

Tue Feb 20 00:00:00 EST 2018

E3 Ligase VHL Promotes Group 2 Innate Lymphoid Cell Maturation and Function via Glycolysis Inhibition and Induction of Interleukin-33 Receptor.

Immunity

Li Q,Li D,Zhang X,Wan Q,Zhang W,Zheng M,Zou L,Elly C,Lee JH,Liu YC

25-9942 was used in Flow Cytometry to indicate that the VHL-HIF-glycolysis axis is essential for the late-stage maturation and function of ILC2s via targeting IL-33-ST2 pathway.

Tue Feb 20 00:00:00 EST 2018

Control of T_reg cell homeostasis and immune equilibrium by Lkb1 in dendritic cells.

Nature communications

Chen S,Fang L,Guo W,Zhou Y,Yu G,Li W,Dong K,Liu J,Luo Y,Wang B,Li Z,Zhao C,Sun Z,Shen Y,Leng Q,Zhou D,Han Z,Huang H,Ren H,Xu G,Feng X

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9942-82) in Flow Cytometry

Thu Dec 13 00:00:00 EST 2018

Roquin targets mRNAs in a 3'-UTR-specific manner by different modes of regulation.

Nature communications

Essig K,Kronbeck N,Guimaraes JC,Lohs C,Schlundt A,Hoffmann A,Behrens G,Brenner S,Kowalska J,Lopez-Rodriguez C,Jemielity J,Holtmann H,Reiche K,Hackermüller J,Sattler M,Zavolan M,Heissmeyer V

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9942-82) in Flow Cytometry

Wed Sep 19 00:00:00 EDT 2018

Targeted calcium influx boosts cytotoxic T lymphocyte function in the tumour microenvironment.

Nature communications

Kim KD,Bae S,Capece T,Nedelkovska H,de Rubio RG,Smrcka AV,Jun CD,Jung W,Park B,Kim TI,Kim M

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9942-82) in Flow Cytometry

Mon May 15 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9942-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD278 (ICOS) monoclonal antibody (Product # 25-9942-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Bioinformatics

Protein Aliases: Activation-inducible lymphocyte immunomediatory molecule; CCLP; CD278; CD28 and CTLA-4-like protein; CD28-related protein 1; CRP-1; inducible T-cell co-stimulator; Inducible T-cell costimulator

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Gene Aliases: AILIM; CCLP; CRP-1; H4; Icos; Ly115

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UniProt ID: (Mouse) Q9WVS0

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Entrez Gene ID: (Mouse) 54167

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Function(s)

protein binding

Process(es)

T cell tolerance induction single organismal cell-cell adhesion T cell costimulation

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD278 (ICOS) Monoclonal Antibody (7E.17G9), PE-Cyanine7, eBioscience™

CD278 (ICOS) Antibody (25-9942-82) in Flow

Product Details

25-9942-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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