Invitrogen

CD3 Monoclonal Antibody (UCHT1), eFluor™ 450, eBioscience™

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This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

14 Published Figures

33 References

View all (504) CD3 antibodies

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CD3 Antibody (48-0038-82) in Flow

Staining of human peripheral blood mononuclear cells with CD45 PE, CD19 APC and CD3 eFluor 450. As expected based on known relative expression patterns, CD3 clone UCHT1 stains a subset of lymphocytes (pink), but not monocytes (orange) and granulocytes (blue).

CD3 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

CD3 Antibody — Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD3 clone UCHT1 stains a subset of lymphocytes (T cells) and does not stain monocytes and granulocytes. Details: Normal human whole blood was surface stained with CD3 (clone UCHT1). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used for analysis. {RE}

Product Details

48-0038-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 32 publications 32 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

UCHT1

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1518799

Product Specific Information

Description: The UCHT1 monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR via immobilized UCHT1 initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.

Applications Reported: This UCHT1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This UCHT1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is a replacement for Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Aberrant gut-microbiota-immune-brain axis development in premature neonates with brain damage.

Cell host & microbe

Seki D,Mayer M,Hausmann B,Pjevac P,Giordano V,Goeral K,Unterasinger L,Klebermaß-Schrehof K,De Paepe K,Van de Wiele T,Spittler A,Kasprian G,Warth B,Berger A,Berry D,Wisgrill L

Published figure using CD3 monoclonal antibody (Product # 48-0038-80) in Flow Cytometry

Wed Oct 13 00:00:00 EDT 2021

Single-cell RNA sequencing reveals ex vivo signatures of SARS-CoV-2-reactive T cells through 'reverse phenotyping'.

Nature communications

Fischer DS,Ansari M,Wagner KI,Jarosch S,Huang Y,Mayr CH,Strunz M,Lang NJ,D'Ippolito E,Hammel M,Mateyka L,Weber S,Wolff LS,Witter K,Fernandez IE,Leuschner G,Milger K,Frankenberger M,Nowak L,Heinig-Menhard K,Koch I,Stoleriu MG,Hilgendorff A,Behr J,Pichlmair A,Schubert B,Theis FJ,Busch DH,Schiller HB,Schober K

Published figure using CD3 monoclonal antibody (Product # 48-0038-80) in Flow Cytometry

Mon Jul 26 00:00:00 EDT 2021

Single-cell transcriptomics reveals regulators underlying immune cell diversity and immune subtypes associated with prognosis in nasopharyngeal carcinoma.

Cell research

Chen YP,Yin JH,Li WF,Li HJ,Chen DP,Zhang CJ,Lv JW,Wang YQ,Li XM,Li JY,Zhang PP,Li YQ,He QM,Yang XJ,Lei Y,Tang LL,Zhou GQ,Mao YP,Wei C,Xiong KX,Zhang HB,Zhu SD,Hou Y,Sun Y,Dean M,Amit I,Wu K,Kuang DM,Li GB,Liu N,Ma J

Published figure using CD3 monoclonal antibody (Product # 48-0038-80) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Aging affects responsiveness of peripheral blood mononuclear cells to immunosuppression of periodontal ligament stem cells.

The Journal of international medical research

Li X,Zhang B,Wang H,Zhao X,Zhang Z,Ding G,Wei F

Published figure using CD3 monoclonal antibody (Product # 48-0038-80) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Involvement of natural killer cells in the pathogenesis of endometriosis in patients with pelvic pain.

The Journal of international medical research

He J,Xu Y,Yi M,Gu C,Zhu Y,Hu G

Published figure using CD3 monoclonal antibody (Product # 48-0038-80) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Dendritic Cell Maturation Regulates TSPAN7 Function in HIV-1 Transfer to CD4⁺ T Lymphocytes.

Frontiers in cellular and infection microbiology

Perot BP,García-Paredes V,Luka M,Ménager MM

Published figure using CD3 monoclonal antibody (Product # 48-0038-80) in Flow Cytometry

Wed Mar 17 00:00:00 EDT 2021

Th2-like T Follicular Helper Cells Promote Functional Antibody Production during Plasmodium falciparum Infection.

Cell reports. Medicine

Chan JA,Loughland JR,de Labastida Rivera F,SheelaNair A,Andrew DW,Dooley NL,Wines BD,Amante FH,Webb L,Hogarth PM,McCarthy JS,Beeson JG,Engwerda CR,Boyle MJ

Published figure using CD3 monoclonal antibody (Product # 48-0038-80) in Flow Cytometry

Tue Dec 22 00:00:00 EST 2020

Loss of ARPC1B impairs cytotoxic T lymphocyte maintenance and cytolytic activity.

The Journal of clinical investigation

Randzavola LO,Strege K,Juzans M,Asano Y,Stinchcombe JC,Gawden-Bone CM,Seaman MN,Kuijpers TW,Griffiths GM

Published figure using CD3 monoclonal antibody (Product # 48-0038-80) in Flow Cytometry

Mon Dec 02 00:00:00 EST 2019

Skewed T cell responses to Epstein-Barr virus in long-term asymptomatic kidney transplant recipients.

PloS one

Nakid-Cordero C,Arzouk N,Gauthier N,Tarantino N,Larsen M,Choquet S,Burrel S,Autran B,Vieillard V,Guihot A

Published figure using CD3 monoclonal antibody (Product # 48-0038-80) in Flow Cytometry

Mon Mar 30 00:00:00 EDT 2020

Inflammation induced by incomplete radiofrequency ablation accelerates tumor progression and hinders PD-1 immunotherapy.

Nature communications

Shi L,Wang J,Ding N,Zhang Y,Zhu Y,Dong S,Wang X,Peng C,Zhou C,Zhou L,Li X,Shi H,Wu W,Long X,Wu C,Liao W

Published figure using CD3 monoclonal antibody (Product # 48-0038-80) in Flow Cytometry

Thu Nov 28 00:00:00 EST 2019

Biallelic mutations in DNA ligase 1 underlie a spectrum of immune deficiencies.

The Journal of clinical investigation

Maffucci P,Chavez J,Jurkiw TJ,O'Brien PJ,Abbott JK,Reynolds PR,Worth A,Notarangelo LD,Felgentreff K,Cortes P,Boisson B,Radigan L,Cobat A,Dinakar C,Ehlayel M,Ben-Omran T,Gelfand EW,Casanova JL,Cunningham-Rundles C

Published figure using CD3 monoclonal antibody (Product # 48-0038-80) in Flow Cytometry

Mon Dec 03 00:00:00 EST 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

48-0038 was used in Flow cytometry/Cell sorting to demonstrate that blocking PITPNM3 recognition of CCL18 could be an attractive immunotherapy aproach for treating breast cancer.

Sat Apr 01 00:00:00 EDT 2017

Follicular Regulatory T Cells Are Highly Permissive to R5-Tropic HIV-1.

Journal of virology

Miller SM,Miles B,Guo K,Folkvord J,Meditz AL,McCarter MD,Levy DN,MaWhinney S,Santiago ML,Connick E

Published figure using CD3 monoclonal antibody (Product # 48-0038-80) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Eomesodermin and T-bet mark developmentally distinct human natural killer cells.

JCI insight

Collins A,Rothman N,Liu K,Reiner SL

48-0038 was used in Flow cytometry/Cell sorting to demonstrate the expression pattern of signature NK cell transcription factors during developmental maturation.

Thu Mar 09 00:00:00 EST 2017

Interaction among activated lymphocytes and mesenchymal cells through podoplanin is critical for a high IL-17 secretion.

Arthritis research & therapy

Noack M,Ndongo-Thiam N,Miossec P

48-0038 was used in Flow cytometry/Cell sorting to study interactions between synoviocytes from rheumatoid arthritis patients and peripheral blood mononuclear cells, and their effect on the Th17 pathway during chronic inflammation.

Thu Jun 23 00:00:00 EDT 2016

Failure recovery of circulating NKG2D⁺CD56^dimNK cells in HBV-associated hepatocellular carcinoma after hepatectomy predicts early recurrence.

Oncoimmunology

Gao J,Duan Z,Zhang L,Huang X,Long L,Tu J,Liang H,Zhang Y,Shen T,Lu F

48-0038 was used in Flow cytometry/Cell sorting to analyse changes in natural killer cell profile in peripheral blood after surgery and in the tumour tissues of hepatocellular carcinoma patients.

Tue Jan 12 00:00:00 EST 2021

MAIT cells are activated during human viral infections.

Nature communications

van Wilgenburg B,Scherwitzl I,Hutchinson EC,Leng T,Kurioka A,Kulicke C,de Lara C,Cole S,Vasanawathana S,Limpitikul W,Malasit P,Young D,Denney L,Moore MD,Fabris P,Giordani MT,Oo YH,Laidlaw SM,Dustin LB,Ho LP,Thompson FM,Ramamurthy N,Mongkolsapaya J,Willberg CB,Screaton GR,Klenerman P

48-0038 was used in Flow cytometry/Cell sorting to demonstrate how MAIT cells are activated following viral infections, suggesting a potential role in both host defence and immunopathology.

Thu Jun 23 00:00:00 EDT 2016

Antitumor immunity triggered by melphalan is potentiated by melanoma cell surface-associated calreticulin.

Cancer research

Dudek-Perić AM,Ferreira GB,Muchowicz A,Wouters J,Prada N,Martin S,Kiviluoto S,Winiarska M,Boon L,Mathieu C,van den Oord J,Stas M,Gougeon ML,Golab J,Garg AD,Agostinis P

48-0038 was used in Flow cytometry/Cell sorting to illustrate how melphalan triggers inflammatory cell death that can be leveraged by immunomodulators.

Wed Apr 15 00:00:00 EDT 2015

CD8+ T Cells Can Mediate Short-Term Protection against Heterotypic Dengue Virus Reinfection in Mice.

Journal of virology

Zellweger RM,Tang WW,Eddy WE,King K,Sanchez MC,Shresta S

48-0038 was used in Flow cytometry/Cell sorting to investigate short-term protection against heterotypic Dengue virus reinfection in mice mediated by CD8+ T Cells.

Mon Jun 01 00:00:00 EDT 2015

Vitamin D deficiency contributes to the reduction and impaired function of naïve CD45RA⁺ regulatory T cell in chronic heart failure.

Journal of immunology research

Ma YH,Zhou YL,Yue CY,Zhang GH,Deng L,Xie GH,Xu WP,Shen LS

48-0038 was used in Flow cytometry/Cell sorting to investigate the impact of vitamin D supplementation on naïve Tregs.

Tue Jan 26 00:00:00 EST 2016

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using CD3 monoclonal antibody (Product # 48-0038-80) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

Children with asthma by school age display aberrant immune responses to pathogenic airway bacteria as infants.

The Journal of allergy and clinical immunology

Larsen JM,Brix S,Thysen AH,Birch S,Rasmussen MA,Bisgaard H

48-0038 was used in Flow cytometry/Cell sorting to assess the immune responses to pathogen bacteria in children suffering from asthma.

Tue Apr 01 00:00:00 EDT 2014

Monocyte-activation phenotypes are associated with biomarkers of inflammation and coagulation in chronic HIV infection.

The Journal of infectious diseases

Wilson EM,Singh A,Hullsiek KH,Gibson D,Henry WK,Lichtenstein K,Önen NF,Kojic E,Patel P,Brooks JT,Sereti I,Baker JV

48-0038 was used in Flow cytometry/Cell sorting to indicate the association of monocyte-activation phenotypes with biomarkers of inflammation and coagulation in chronic HIV infection.

Sat Nov 01 00:00:00 EDT 2014

Evidence for innate immune system activation in HIV type 1-infected elite controllers.

The Journal of infectious diseases

Krishnan S,Wilson EM,Sheikh V,Rupert A,Mendoza D,Yang J,Lempicki R,Migueles SA,Sereti I

48-0038 was used in Flow cytometry/Cell sorting to study how elite controllers maintain control of plasma HIV viremia and have evidence of an activated innate immune response.

Sat Mar 01 00:00:00 EST 2014

Decreases in colonic and systemic inflammation in chronic HIV infection after IL-7 administration.

PLoS pathogens

Sereti I,Estes JD,Thompson WL,Morcock DR,Fischl MA,Croughs T,Beq S,Lafaye de Micheaux S,Yao MD,Ober A,Wilson EM,Natarajan V,Imamichi H,Boulassel MR,Lederman MM,Routy JP

48-0038 was used in Flow cytometry/Cell sorting to test the efficacy of IL-7 adjunctive therapy on T-cell reconstitution in antiretroviral therapy-suppressed HIV patients.

Wed Jan 01 00:00:00 EST 2014

Human mesenchymal stromal cells modulate T-cell responses through TNF-α-mediated activation of NF-κB.

European journal of immunology

Dorronsoro A,Ferrin I,Salcedo JM,Jakobsson E,Fernández-Rueda J,Lang V,Sepulveda P,Fechter K,Pennington D,Trigueros C

Published figure using CD3 monoclonal antibody (Product # 48-0038-80) in Flow Cytometry

Sat Feb 01 00:00:00 EST 2014

A truncation variant of the cation channel P2RX5 is upregulated during T cell activation.

PloS one

Abramowski P,Ogrodowczyk C,Martin R,Pongs O

48-0038 was used in Flow cytometry/Cell sorting to show that P2RX5 gene expression of human T lymphocytes is upregulated during activation.

Mon May 18 00:00:00 EDT 2015

ROS-induced autophagy in cancer cells assists in evasion from determinants of immunogenic cell death.

Autophagy

Garg AD,Dudek AM,Ferreira GB,Verfaillie T,Vandenabeele P,Krysko DV,Mathieu C,Agostinis P

48-0038 was used in Flow cytometry/Cell sorting to unravel a role for ROS-induced autophagy in weakening functional interaction between dying cancer cells and the immune system.

Sun Sep 01 00:00:00 EDT 2013

Comprehensive analysis of dengue virus-specific responses supports an HLA-linked protective role for CD8+ T cells.

Proceedings of the National Academy of Sciences of the United States of America

Weiskopf D,Angelo MA,de Azeredo EL,Sidney J,Greenbaum JA,Fernando AN,Broadwater A,Kolla RV,De Silva AD,de Silva AM,Mattia KA,Doranz BJ,Grey HM,Shresta S,Peters B,Sette A

48-0038 was used in Flow cytometry/Cell sorting to analyse dengue virus-specific responses, supporting an HLA-linked protective role for CD8+ T cells.

Tue May 28 00:00:00 EDT 2013

Memory T cells in latent Mycobacterium tuberculosis infection are directed against three antigenic islands and largely contained in a CXCR3+CCR6+ Th1 subset.

PLoS pathogens

Lindestam Arlehamn CS,Gerasimova A,Mele F,Henderson R,Swann J,Greenbaum JA,Kim Y,Sidney J,James EA,Taplitz R,McKinney DM,Kwok WW,Grey H,Sallusto F,Peters B,Sette A

48-0038 was used in Flow cytometry/Cell sorting to further define the immunological footprint of Mycobacterium tuberculosis CD4 T cell recognition.

Tue Jan 01 00:00:00 EST 2013

Ribavirin Does Not Impair the Suppressive Activity of Foxp3(+)CD4(+)CD25(+) Regulatory T Cells.

Immune network

Lee J,Choi YS,Shin EC

48-0038 was used in Flow cytometry/Cell sorting to demonstrate that ribavirin did not attenuate the suppressive activity of Foxp3(+)CD4(+)CD25(+) Treg cells.

Fri Feb 01 00:00:00 EST 2013

Characterization of Th17 responses to Streptococcus pneumoniae in humans: comparisons between adults and children in a developed and a developing country.

Vaccine

Lundgren A,Bhuiyan TR,Novak D,Kaim J,Reske A,Lu YJ,Qadri F,Malley R

48-0038 was used in Flow cytometry/Cell sorting to compare different Th17 responses to pneumococcal antigens and whole cell antigens, in children and adults in both developed and developing countries.

Wed Jun 06 00:00:00 EDT 2012

Expansion of regulatory T cells in patients with Langerhans cell histiocytosis.

PLoS medicine

Senechal B,Elain G,Jeziorski E,Grondin V,Patey-Mariaud de Serre N,Jaubert F,Beldjord K,Lellouch A,Glorion C,Zerah M,Mary P,Barkaoui M,Emile JF,Boccon-Gibod L,Josset P,Debré M,Fischer A,Donadieu J,Geissmann F

Published figure using CD3 monoclonal antibody (Product # 48-0038-80) in Immunohistochemistry

Wed Aug 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: CD247; CD247 antigen, zeta subunit; CD3 antigen, delta subunit; CD3 delta; CD3 TCR complex; CD3-epsilon; CD3d; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3e; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3g; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3Z antigen, zeta polypeptide (TiT3 complex); CD3zeta chain; FLJ18683; Leu-4; OKT3, delta chain; T cell antigen receptor complex epsilon subunit of T3; T-cell antigen receptor complex, epsilon subunit of T3; T-cell antigen receptor complex, gamma subunit of T3; T-cell antigen receptor complex, zeta subunit of CD3; T-cell receptor T3 delta chain; T-cell receptor T3 gamma chain; T-cell receptor T3 zeta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; T-cell surface glycoprotein CD3 gamma chain; T-cell surface glycoprotein CD3 zeta chain; T3/TCR complex; TCR zeta chain

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Gene Aliases: CD247; CD3-DELTA; CD3-GAMMA; CD3-ZETA; CD3D; CD3E; CD3G; CD3H; CD3Q; CD3Z; IMD17; IMD18; IMD19; IMD25; T3D; T3E; T3G; T3Z; TCRE; TCRZ

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UniProt ID: (Human) P20963, (Human) P04234, (Human) P07766, (Human) P09693

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Entrez Gene ID: (Human) 919, (Human) 915, (Human) 916, (Human) 917

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Function(s)

transmembrane signaling receptor activity protein binding identical protein binding protein homodimerization activity transcription coactivator activity protein heterodimerization activity receptor signaling protein activity SH3 domain binding protein kinase binding receptor signaling complex scaffold activity T cell receptor binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

Performance Guarantee

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CD3 Monoclonal Antibody (UCHT1), eFluor™ 450, eBioscience™

CD3 Antibody (48-0038-82) in Flow

Product Details

48-0038-82

Immunohistochemistry (IHC)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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