Invitrogen

CD4 Monoclonal Antibody (RM4-5), APC, eBioscience™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

96 Published Figures

229 References

View all (606) CD4 antibodies

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CD4 Antibody (17-0042-82) in Flow

Staining of mouse splenocytes with Anti-Mouse CD3 FITC (Product # 11-0032-82) and 0.06 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.06 µg of Anti-Mouse CD4 APC (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD4 Antibody — Staining of mouse splenocytes and bone marrow cells. Right: As expected based on known relative expression patterns, CD4 clone RM4-5 stains a subset of splenocytes and does not stain any bone marrow cells. Details: Balb/c bone marrow cells (left) and splenocytes (middle) were surface stained with CD4 (clone RM4-5) followed by staining with 7-AAD. Viable bone marrow cells in the lymphoid (blue histogram) and myeloid (purple histogram) gates and viable splenocytes (orange histogram) were used for analysis. {RE}

Product Details

17-0042-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunohistochemistry (Frozen) (IHC (F))

View 3 publications 3 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.125 µg/test

View 223 publications 223 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RM4-5

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_469323

Product Specific Information

Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Reported: The RM4-5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: The RM4-5 antibody has been tested by flow cytometric analysis of mouse thymocyte or splenocyte suspensions. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Impact of broad-spectrum antibiotics on the gut-microbiota-spleen-brain axis.

Brain, behavior, & immunity - health

Wan X,Eguchi A,Sakamoto A,Fujita Y,Yang Y,Qu Y,Hatano M,Mori C,Hashimoto K

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2023

Impact of broad-spectrum antibiotics on the gut-microbiota-spleen-brain axis.

Brain, behavior, & immunity - health

Wan X,Eguchi A,Sakamoto A,Fujita Y,Yang Y,Qu Y,Hatano M,Mori C,Hashimoto K

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2023

Spleen-targeted neoantigen DNA vaccine for personalized immunotherapy of hepatocellular carcinoma.

EMBO molecular medicine

Wu M,Luo Z,Cai Z,Mao Q,Li Z,Li H,Zhang C,Zhang Y,Zhong A,Wu L,Liu X

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Oct 11 00:00:00 EDT 2023

CXCR1 drives the pathogenesis of EAE and ARDS via boosting dendritic cells-dependent inflammation.

Cell death & disease

Zhuang W,Zhou J,Zhong L,Lv J,Zhong X,Liu G,Xie L,Wang C,Saimaier K,Han S,Shi C,Hua Q,Zhang R,Xie X,Du C

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Sep 14 00:00:00 EDT 2023

Platelet factors attenuate inflammation and rescue cognition in ageing.

Nature

Schroer AB,Ventura PB,Sucharov J,Misra R,Chui MKK,Bieri G,Horowitz AM,Smith LK,Encabo K,Tenggara I,Couthouis J,Gross JD,Chan JM,Luke A,Villeda SA

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2023

Klebsiella pneumoniae Induces Inflammatory Bowel Disease Through Caspase-11-Mediated IL18 in the Gut Epithelial Cells.

Cellular and molecular gastroenterology and hepatology

Zhang Q,Su X,Zhang C,Chen W,Wang Y,Yang X,Liu D,Zhang Y,Yang R

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Feb 23 00:00:00 EST 2023

Icariin restrains NLRP3 inflammasome-mediated Th2 immune responses and ameliorates atopic dermatitis through modulating a novel lncRNA MALAT1/miR-124-3p axis.

Pharmaceutical biology

Zhao W,Yu HH,Meng WW,Liu AM,Zhang BX,Wang Y,Li J,Wang L,Fang YF

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Dec 01 00:00:00 EST 2023

Regulatory T cells require IL6 receptor alpha signaling to control skeletal muscle function and regeneration.

Cell metabolism

Becker M,Joseph SS,Garcia-Carrizo F,Tom RZ,Opaleva D,Serr I,Tschöp MH,Schulz TJ,Hofmann SM,Daniel C

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Oct 03 00:00:00 EDT 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Palmitoyl transferases act as novel drug targets for pancreatic cancer.

Journal of translational medicine

Lin Z,Lv Z,Liu X,Huang K

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Apr 10 00:00:00 EDT 2023

SARS-CoV-2 spike protein promotes inflammatory cytokine activation and aggravates rheumatoid arthritis.

Cell communication and signaling : CCS

Lee AR,Woo JS,Lee SY,Lee YS,Jung J,Lee CR,Park SH,Cho ML

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Mar 02 00:00:00 EST 2023

TRAF6 controls T cell homeostasis by maintaining the equilibrium of MALT1 scaffolding and protease functions.

Frontiers in immunology

O'Neill TJ,Gewies A,Seeholzer T,Krappmann D

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Methyl-cpg-binding Domain Protein 2 Silencing Inhibits Th17 Differentiation of CD4+T cells Induced by Ovalbumin.

Iranian journal of immunology : IJI

Jiang Y,Li L,Pan Q,Du X,Han Q,Ling F,Li R,Mai L,Huang J,Chu S,Ma L

17-0042-82 was used in Flow Cytometry to explore the mechanism of methyl-cpg-binding domain protein 2 (MBD2) in CD4+T cell differentiation stimulated by environmental allergen ovalbumin (OVA).

Tue Mar 14 00:00:00 EDT 2023

Meningeal lymphatics regulate radiotherapy efficacy through modulating anti-tumor immunity.

Cell research

Zhou C,Ma L,Xu H,Huo Y,Luo J

17-0042-82 was used in Flow Cytometry to suggest an essential role of the MLV-CLN network in RT-triggered anti-tumor immunity, and highlight the potential of VEGF-C mRNA for brain tumor therapy.

Wed Jun 01 00:00:00 EDT 2022

Aryl hydrocarbon receptor activation ameliorates experimental colitis by modulating the tolerogenic dendritic and regulatory T cell formation.

Cell & bioscience

Cui X,Ye Z,Wang D,Yang Y,Jiao C,Ma J,Tang N,Zhang H

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sat Apr 23 00:00:00 EDT 2022

IL-33 induces thymic involution-associated naive T cell aging and impairs host control of severe infection.

Nature communications

Xu L,Wei C,Chen Y,Wu Y,Shou X,Chen W,Lu D,Sun H,Li W,Yu B,Wang X,Zhang X,Yu Y,Lei Z,Tang R,Zhu J,Li Y,Lu L,Zhou H,Zhou S,Su C,Chen X

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sat Nov 12 00:00:00 EST 2022

Fringe GlcNAc-transferases differentially extend O-fucose on endogenous NOTCH1 in mouse activated T cells.

The Journal of biological chemistry

Matsumoto K,Kumar V,Varshney S,Nairn AV,Ito A,Pennarubia F,Moremen KW,Stanley P,Haltiwanger RS

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2022

Breast cancer cell-derived extracellular vesicles promote CD8⁺ T cell exhaustion via TGF-β type II receptor signaling.

Nature communications

Xie F,Zhou X,Su P,Li H,Tu Y,Du J,Pan C,Wei X,Zheng M,Jin K,Miao L,Wang C,Meng X,van Dam H,Ten Dijke P,Zhang L,Zhou F

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

A nonredundant role for T cell-derived interleukin 22 in antibacterial defense of colonic crypts.

Immunity

Zindl CL,Witte SJ,Laufer VA,Gao M,Yue Z,Janowski KM,Cai B,Frey BF,Silberger DJ,Harbour SN,Singer JR,Turner H,Lund FE,Vallance BA,Rosenberg AF,Schoeb TR,Chen JY,Hatton RD,Weaver CT

17-0042-82 was used in Flow cytometry/Cell sorting to demonstrate spatiotemporal differences in the production and action of IL-22 by ILCs and T cells during infection and reveal an indispensable role for IL-22-producing T cells in the protection of the intestinal crypts.

Tue Mar 08 00:00:00 EST 2022

Foxo3a tempers excessive glutaminolysis in activated T cells to prevent fatal gut inflammation in the murine IL-10^-/- model of colitis.

Cell death and differentiation

Hajjar S,Nathan N,Joseph J,Mottawea W,Ariana A,Pyatibrat S,Harper ME,Alain T,Blais A,Russell RC,Sad S

17-0042-82 was used in Flow cytometry/Cell sorting to show that Foxo3a restricts the abundance of colitogenic microbiota in IL-10-deficient mice.

Tue Mar 01 00:00:00 EST 2022

Induction of osteogenesis by bone-targeted Notch activation.

eLife

Xu C,Dinh VV,Kruse K,Jeong HW,Watson EC,Adams S,Berkenfeld F,Stehling M,Rasouli SJ,Fan R,Chen R,Bedzhov I,Chen Q,Kato K,Pitulescu ME,Adams RH

17-0042-82 was used in Flow Cytometry to propose that activation of Notch signaling by bone-targeted fusion proteins might be therapeutically useful and can avoid detrimental effects in Notch-dependent processes in other organs.

Fri Feb 04 00:00:00 EST 2022

Axl regulated survival/proliferation network and its therapeutic intervention in mouse models of glomerulonephritis.

Arthritis research & therapy

Zhen Y,Ren Y,Medvedovic M,Adams DE,Wang D,Shao WH

17-0042-82 was used in Immunohistochemistry (Frozen) to analyze the gene expression profiles with RNA-seq using renal cortical samples from nephritic mice.

Wed Dec 28 00:00:00 EST 2022

Loss of Optineurin Drives Cancer Immune Evasion via Palmitoylation-Dependent IFNGR1 Lysosomal Sorting and Degradation.

Cancer discovery

Du W,Hua F,Li X,Zhang J,Li S,Wang W,Zhou J,Wang W,Liao P,Yan Y,Li G,Wei S,Grove S,Vatan L,Zgodziński W,Majewski M,Wallner G,Chen H,Kryczek I,Fang JY,Zou W

17-0042-82 was used in Flow Cytometry to show that optineurin is a shared node between the IFN and MHC pathways and predicts colorectal cancer patient outcome.

Thu Jul 01 00:00:00 EDT 2021

Niche-specific MHC II and PD-L1 regulate CD4+CD8αα+ intraepithelial lymphocyte differentiation.

The Journal of experimental medicine

Moon S,Park Y,Hyeon S,Kim YM,Kim JH,Kim H,Park S,Lee KJ,Koo BK,Ha SJ,Lee SW

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Apr 05 00:00:00 EDT 2021

Unexpected PD-L1 immune evasion mechanism in TNBC, ovarian, and other solid tumors by DR5 agonist antibodies.

EMBO molecular medicine

Mondal T,Shivange GN,Tihagam RG,Lyerly E,Battista M,Talwar D,Mosavian R,Urbanek K,Rashid NS,Harrell JC,Bos PD,Stelow EB,Stack MS,Bhatnagar S,Tushir-Singh J

17-0042-82 was used in Flow Cytometry to identify a potential clinically viable combinatorial strategy to revive solid cancer immunotherapy using death receptor agonism.

Fri Mar 05 00:00:00 EST 2021

Romidepsin (FK228) regulates the expression of the immune checkpoint ligand PD-L1 and suppresses cellular immune functions in colon cancer.

Cancer immunology, immunotherapy : CII

Shi Y,Fu Y,Zhang X,Zhao G,Yao Y,Guo Y,Ma G,Bai S,Li H

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Potential therapeutic effects of interleukin-35 on the differentiation of naïve T cells into Helios⁺Foxp3⁺ Tregs in clinical and experimental acute respiratory distress syndrome.

Molecular immunology

Wang C,Xie K,Li K,Lin S,Xu F

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Anisakis pegreffii Extract Induces Airway Inflammation with Airway Remodeling in a Murine Model System.

BioMed research international

Choi JH,Kim JY,Yi MH,Kim M,Yong TS

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Jan 12 00:00:00 EST 2022

Anisakis pegreffii Extract Induces Airway Inflammation with Airway Remodeling in a Murine Model System.

BioMed research international

Choi JH,Kim JY,Yi MH,Kim M,Yong TS

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Jan 12 00:00:00 EST 2022

Feline adipose tissue-derived mesenchymal stem cells pretreated with IFN-γ enhance immunomodulatory effects through the PGE₂ pathway.

Journal of veterinary science

Park SG,An JH,Li Q,Chae HK,Park SM,Lee JH,Ahn JO,Song WJ,Youn HY

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Intratumoral expression of interleukin 23 variants using oncolytic vaccinia virus elicit potent antitumor effects on multiple tumor models via tumor microenvironment modulation.

Theranostics

Chen L,Chen H,Ye J,Ge Y,Wang H,Dai E,Ren J,Liu W,Ma C,Ju S,Guo ZS,Liu Z,Bartlett DL

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Aug 04 00:00:00 EDT 2021

Intratumoral expression of interleukin 23 variants using oncolytic vaccinia virus elicit potent antitumor effects on multiple tumor models via tumor microenvironment modulation.

Theranostics

Chen L,Chen H,Ye J,Ge Y,Wang H,Dai E,Ren J,Liu W,Ma C,Ju S,Guo ZS,Liu Z,Bartlett DL

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Aug 04 00:00:00 EDT 2021

Antitumor efficacy of a neoantigen cancer vaccine delivered by electroporation is influenced by microbiota composition.

Oncoimmunology

Lione L,Salvatori E,Petrazzuolo A,Massacci A,Maggio R,Confroti A,Compagnone M,Aurisicchio L,Ciliberto G,Palombo F

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Mar 23 00:00:00 EDT 2021

Evaluation of DNA-Launched Virus-Like Particle Vaccines in an Immune Competent Mouse Model of Chikungunya Virus Infection.

Vaccines

Rayner JO,Kim JH,Roberts RW,Wood RR,Fouty B,Solodushko V

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Apr 02 00:00:00 EDT 2021

Ddb1 Is Essential for the Expansion of CD4⁺ Helper T Cells by Regulating Cell Cycle Progression and Cell Death.

Frontiers in immunology

Yang L,Chen W,Li L,Xiao Y,Fan S,Zhang Q,Xia T,Li M,Hong Y,Zhao T,Li Q,Liu WH,Xiao N

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Dec 20 00:00:00 EST 2021

Ddb1 Is Essential for the Expansion of CD4⁺ Helper T Cells by Regulating Cell Cycle Progression and Cell Death.

Frontiers in immunology

Yang L,Chen W,Li L,Xiao Y,Fan S,Zhang Q,Xia T,Li M,Hong Y,Zhao T,Li Q,Liu WH,Xiao N

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Dec 20 00:00:00 EST 2021

Gut Microbiota Dysbiosis Induced by Intracerebral Hemorrhage Aggravates Neuroinflammation in Mice.

Frontiers in microbiology

Yu X,Zhou G,Shao B,Zhou H,Xu C,Yan F,Wang L,Chen G,Li J,Fu X

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed May 26 00:00:00 EDT 2021

Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials.

EBioMedicine

Okada R,Furusawa A,Vermeer DW,Inagaki F,Wakiyama H,Kato T,Nagaya T,Choyke PL,Spanos WC,Allen CT,Kobayashi H

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Activation of Bone Marrow Adaptive Immunity in Type 2 Diabetes: Rescue by Co-stimulation Modulator Abatacept.

Frontiers in immunology

Santopaolo M,Sullivan N,Thomas AC,Alvino VV,Nicholson LB,Gu Y,Spinetti G,Kallikourdis M,Blom A,Madeddu P

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Jul 02 00:00:00 EDT 2021

Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer.

Nature communications

Huang Y,Guan Z,Dai X,Shen Y,Wei Q,Ren L,Jiang J,Xiao Z,Jiang Y,Liu D,Huang Z,Xu X,Luo Y,Zhao C

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Histological, behavioural and flow cytometric datasets relating to acute ischaemic stroke in young, aged and ApoE^-/- mice in the presence and absence of immunomodulation with fingolimod.

Data in brief

Diaz Diaz AC,Malone K,Shearer JA,Moore AC,Waeber C

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Local Sustained GM-CSF Delivery by Genetically Engineered Encapsulated Cells Enhanced Both Cellular and Humoral SARS-CoV-2 Spike-Specific Immune Response in an Experimental Murine Spike DNA Vaccination Model.

Vaccines

Vernet R,Charrier E,Cosset E,Fièvre S,Tomasello U,Grogg J,Mach N

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon May 10 00:00:00 EDT 2021

Sequencing, cloning, and antigen binding analysis of monoclonal antibodies isolated from single mouse B cells.

STAR protocols

Viant C,Escolano A,Chen ST,Nussenzweig MC

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Jun 18 00:00:00 EDT 2021

Small-molecule antagonist of VLA-4 (GW559090) attenuated neuro-inflammation by targeting Th17 cell trafficking across the blood-retinal barrier in experimental autoimmune uveitis.

Journal of neuroinflammation

Chen YH,Eskandarpour M,Zhang X,Galatowicz G,Greenwood J,Lightman S,Calder V

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Feb 18 00:00:00 EST 2021

Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry.

STAR protocols

Skordos I,Demeyer A,Beyaert R

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

STING activation normalizes the intraperitoneal vascular-immune microenvironment and suppresses peritoneal carcinomatosis of colon cancer.

Journal for immunotherapy of cancer

Lee SJ,Yang H,Kim WR,Lee YS,Lee WS,Kong SJ,Lee HJ,Kim JH,Cheon J,Kang B,Chon HJ,Kim C

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers.

PloS one

Cohen AA,Yang Z,Gnanapragasam PNP,Ou S,Dam KA,Wang H,Bjorkman PJ

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Oct 15 00:00:00 EDT 2021

A dysbiotic gut microbiome suppresses antibody mediated-protection against Vibrio cholerae.

iScience

Macbeth JC,Liu R,Alavi S,Hsiao A

17-0042-82 was used in Flow cytometry/Cell sorting to suggest that gut microbiome composition at time of infection or vaccination may be pivotal for providing robust mucosal immunity, and suggest a target for improved prophylactic and therapeutic strategies for cholera.

Fri Dec 17 00:00:00 EST 2021

Restraining Lysosomal Activity Preserves Hematopoietic Stem Cell Quiescence and Potency.

Cell stem cell

Liang R,Arif T,Kalmykova S,Kasianov A,Lin M,Menon V,Qiu J,Bernitz JM,Moore K,Lin F,Benson DL,Tzavaras N,Mahajan M,Papatsenko D,Ghaffari S

17-0042-82 was used in Flow Cytometry to investigate the overall metabolic properties of hematopoietic stem cells.

Thu Mar 05 00:00:00 EST 2020

Protein arginine methyltransferase 5 promotes cholesterol biosynthesis-mediated Th17 responses and autoimmunity.

The Journal of clinical investigation

Webb LM,Sengupta S,Edell C,Piedra-Quintero ZL,Amici SA,Miranda JN,Bevins M,Kennemer A,Laliotis G,Tsichlis PN,Guerau-de-Arellano M

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Long-term ex vivo expansion of mouse hematopoietic stem cells.

Nature protocols

Wilkinson AC,Ishida R,Nakauchi H,Yamazaki S

17-0042 was used in Flow cytometry/Cell sorting to describe a simple platform for the expansion of functional mouse hematopoietic stem cells ex vivo for >1 month under fully defined albumin-free conditions.

Sat Feb 01 00:00:00 EST 2020

Methnaridine is an orally bioavailable, fast-killing and long-acting antimalarial agent that cures Plasmodium infections in mice.

British journal of pharmacology

Wang W,Yao J,Chen Z,Sun Y,Shi Y,Wei Y,Zhou H,Yu Y,Li S,Duan L

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

DKK2 blockage-mediated immunotherapy enhances anti-angiogenic therapy of Kras mutated colorectal cancer.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Hu J,Wang Z,Chen Z,Li A,Sun J,Zheng M,Wu J,Shen T,Qiao J,Li L,Li B,Wu D,Xiao Q

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Increased Tim-3 expression on TILs during treatment with the Anchored GM-CSF vaccine and anti-PD-1 antibodies is inversely correlated with response in prostate cancer.

Journal of Cancer

Zhang X,Chen H,Li G,Zhou X,Shi Y,Zou F,Chen Y,Gao J,Yang S,Wu S,Long Z

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Comparison of rapamycin and methylprednisolone for treating inflammatory muscle disease in a murine model of experimental autoimmune myositis.

Experimental and therapeutic medicine

Kang J,Feng D,Yang F,Tian X,Han W,Jia H

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Effects of CD4⁺ T lymphocytes from ovariectomized mice on bone marrow mesenchymal stem cell proliferation and osteogenic differentiation.

Experimental and therapeutic medicine

Shao BY,Wang L,Yu Y,Chen L,Gan N,Huang WM

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Effects of CD4⁺ T lymphocytes from ovariectomized mice on bone marrow mesenchymal stem cell proliferation and osteogenic differentiation.

Experimental and therapeutic medicine

Shao BY,Wang L,Yu Y,Chen L,Gan N,Huang WM

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Oncolytic vaccinia virus delivering tethered IL-12 enhances antitumor effects with improved safety.

Journal for immunotherapy of cancer

Ge Y,Wang H,Ren J,Liu W,Chen L,Chen H,Ye J,Dai E,Ma C,Ju S,Guo ZS,Liu Z,Bartlett DL

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

Oncolytic vaccinia virus delivering tethered IL-12 enhances antitumor effects with improved safety.

Journal for immunotherapy of cancer

Ge Y,Wang H,Ren J,Liu W,Chen L,Chen H,Ye J,Dai E,Ma C,Ju S,Guo ZS,Liu Z,Bartlett DL

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

A novel antibody targeting TIM-3 resulting in receptor internalization for cancer immunotherapy.

Antibody therapeutics

Kuang Z,Li L,Zhang P,Chen B,Wu M,Ni H,Yi S,Zou J,Liu J

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Mechanisms of interleukin 4 mediated increase in efficacy of vaccines against opioid use disorders.

NPJ vaccines

Crouse B,Robinson C,Huseby Kelcher A,Laudenbach M,Abrahante JE,Pravetoni M

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Oct 29 00:00:00 EDT 2020

CD226 Deletion Reduces Type 1 Diabetes in the NOD Mouse by Impairing Thymocyte Development and Peripheral T Cell Activation.

Frontiers in immunology

Shapiro MR,Yeh WI,Longfield JR,Gallagher J,Infante CM,Wellford S,Posgai AL,Atkinson MA,Campbell-Thompson M,Lieberman SM,Serreze DV,Geurts AM,Chen YG,Brusko TM

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed May 26 00:00:00 EDT 2021

Distinct actions of the fermented beverage kefir on host behaviour, immunity and microbiome gut-brain modules in the mouse.

Microbiome

van de Wouw M,Walsh AM,Crispie F,van Leuven L,Lyte JM,Boehme M,Clarke G,Dinan TG,Cotter PD,Cryan JF

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon May 18 00:00:00 EDT 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Aug 27 00:00:00 EDT 2020

Loss of testosterone impairs anti-tumor neutrophil function.

Nature communications

Markman JL,Porritt RA,Wakita D,Lane ME,Martinon D,Noval Rivas M,Luu M,Posadas EM,Crother TR,Arditi M

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Mar 31 00:00:00 EDT 2020

Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses.

Cell reports

Blagih J,Zani F,Chakravarty P,Hennequart M,Pilley S,Hobor S,Hock AK,Walton JB,Morton JP,Gronroos E,Mason S,Yang M,McNeish I,Swanton C,Blyth K,Vousden KH

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2020

Cancer-Specific Loss of p53 Leads to a Modulation of Myeloid and T Cell Responses.

Cell reports

Blagih J,Zani F,Chakravarty P,Hennequart M,Pilley S,Hobor S,Hock AK,Walton JB,Morton JP,Gronroos E,Mason S,Yang M,McNeish I,Swanton C,Blyth K,Vousden KH

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Jan 14 00:00:00 EST 2020

TIAM2S Mediates Serotonin Homeostasis and Provokes a Pro-Inflammatory Immune Microenvironment Permissive for Colorectal Tumorigenesis.

Cancers

Chan YL,Lai WC,Chen JS,Tseng JT,Chuang PC,Jou J,Lee CT,Sun HS

17-0042 was used in Flow cytometry/Cell sorting to suggest that TIAM2S provokes a pro-inflammatory immune microenvironment permissive to colorectal tumorigenesis through the serotonin-induced immunomodulatory effects.

Wed Jul 08 00:00:00 EDT 2020

KLF10 Deficiency in CD4⁺ T Cells Triggers Obesity, Insulin Resistance, and Fatty Liver.

Cell reports

Wara AK,Wang S,Wu C,Fang F,Haemmig S,Weber BN,Aydogan CO,Tesmenitsky Y,Aliakbarian H,Hawse JR,Subramaniam M,Zhao L,Sage PT,Tavakkoli A,Garza A,Lynch L,Banks AS,Feinberg MW

17-0042-82 was used in Flow Cytometry to explore the role of KLF10 in high-fat-diet-induced obesity and insulin resistance.

Tue Dec 29 00:00:00 EST 2020

A Commercial Probiotic Induces Tolerogenic and Reduces Pathogenic Responses in Experimental Autoimmune Encephalomyelitis.

Cells

Calvo-Barreiro L,Eixarch H,Ponce-Alonso M,Castillo M,Lebrón-Galán R,Mestre L,Guaza C,Clemente D,Del Campo R,Montalban X,Espejo C

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Apr 07 00:00:00 EDT 2020

Mitochondrial Oxidative Phosphorylation Regulates the Fate Decision between Pathogenic Th17 and Regulatory T Cells.

Cell reports

Shin B,Benavides GA,Geng J,Koralov SB,Hu H,Darley-Usmar VM,Harrington LE

17-0042 was used in Flow cytometry/Cell sorting to study the role of mitochondrial oxidative phosphorylation in pathogenic Th17 differentiation.

Tue Feb 11 00:00:00 EST 2020

Resolving Fates and Single-Cell Transcriptomes of Hematopoietic Stem Cell Clones by PolyloxExpress Barcoding.

Cell stem cell

Pei W,Shang F,Wang X,Fanti AK,Greco A,Busch K,Klapproth K,Zhang Q,Quedenau C,Sauer S,Feyerabend TB,Höfer T,Rodewald HR

17-0042 was used in Flow cytometry/Cell sorting to develop the PolyloxExpress allele, enabling Cre-recombinase-dependent RNA barcoding in situ.

Thu Sep 03 00:00:00 EDT 2020

Axenic Caenorhabditis elegans antigen protects against development of type-1 diabetes in NOD mice.

Journal of translational autoimmunity

Jackson-Thompson BM,Torrero M,Mitre BK,Long J,Packiam M,Mitre E

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Long-term ex vivo haematopoietic-stem-cell expansion allows nonconditioned transplantation.

Nature

Wilkinson AC,Ishida R,Kikuchi M,Sudo K,Morita M,Crisostomo RV,Yamamoto R,Loh KM,Nakamura Y,Watanabe M,Nakauchi H,Yamazaki S

17-0042 was used in Flow cytometry/Cell sorting to identify polyvinyl alcohol as a functionally superior replacement for serum albumin that is compatible with good manufacturing practice.

Mon Jul 01 00:00:00 EDT 2019

The cis-Regulatory Atlas of the Mouse Immune System.

Cell

Yoshida H,Lareau CA,Ramirez RN,Rose SA,Maier B,Wroblewska A,Desland F,Chudnovskiy A,Mortha A,Dominguez C,Tellier J,Kim E,Dwyer D,Shinton S,Nabekura T,Qi Y,Yu B,Robinette M,Kim KW,Wagers A,Rhoads A,Nutt SL,Brown BD,Mostafavi S,Buenrostro JD,Benoist C

17-0042-82 was used in Flow Cytometry to generate matched epigenome and transcriptome measurements in 86 primary cell types that span the mouse immune system and its differentiation cascade.

Thu Feb 07 00:00:00 EST 2019

Clonal copy-number mosaicism in autoreactive T lymphocytes in diabetic NOD mice.

Genome research

Alriyami M,Marchand L,Li Q,Du X,Olivier M,Polychronakos C

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

Podoplanin Positive Myeloid Cells Promote Glioma Development by Immune Suppression.

Frontiers in oncology

Eisemann T,Costa B,Peterziel H,Angel P

17-0042 was used in Flow cytometry/Cell sorting to identify the role of podoplanin positive myeloid cells in immune suppression and thus their potential as a target for immune mediated tumour elimination.

Wed Sep 30 00:00:00 EDT 2020

A unique mutator phenotype reveals complementary oncogenic lesions leading to acute leukemia.

JCI insight

Yin M,Baslan T,Walker RL,Zhu YJ,Freeland A,Matsukawa T,Sridharan S,Nussenzweig A,Pruitt SC,Lowe SW,Meltzer PS,Aplan PD

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Dec 05 00:00:00 EST 2019

Mutation of an L-Type Calcium Channel Gene Leads to T Lymphocyte Dysfunction.

Frontiers in immunology

Fenninger F,Han J,Stanwood SR,Nohara LL,Arora H,Choi KB,Munro L,Pfeifer CG,Shanina I,Horwitz MS,Jefferies WA

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Oct 29 00:00:00 EDT 2020

Systemic inhibition of tissue-nonspecific alkaline phosphatase alters the brain-immune axis in experimental sepsis.

Scientific reports

Brichacek AL,Benkovic SA,Chakraborty S,Nwafor DC,Wang W,Jun S,Dakhlallah D,Geldenhuys WJ,Pinkerton AB,Millán JL,Brown CM

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Dec 11 00:00:00 EST 2019

Ufbp1 promotes plasma cell development and ER expansion by modulating distinct branches of UPR.

Nature communications

Zhu H,Bhatt B,Sivaprakasam S,Cai Y,Liu S,Kodeboyina SK,Patel N,Savage NM,Sharma A,Kaufman RJ,Li H,Singh N

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Mar 06 00:00:00 EST 2019

Restricting Glycolysis Preserves T Cell Effector Functions and Augments Checkpoint Therapy.

Cell reports

Renner K,Bruss C,Schnell A,Koehl G,Becker HM,Fante M,Menevse AN,Kauer N,Blazquez R,Hacker L,Decking SM,Bohn T,Faerber S,Evert K,Aigle L,Amslinger S,Landa M,Krijgsman O,Rozeman EA,Brummer C,Siska PJ,Singer K,Pektor S,Miederer M,Peter K,Gottfried E,Herr W,Marchiq I,Pouyssegur J,Roush WR,Ong S,Warren S,Pukrop T,Beckhove P,Lang SA,Bopp T,Blank CU,Cleveland JL,Oefner PJ,Dettmer K,Selby M,Kreutz M

17-0042-82 was used in Flow Cytometry to report that melanoma patients with high expression of glycolysis-related genes show a worse progression free survival upon anti-PD1 treatment and support the rationale for targeting glycolysis in patients with high glycolytic tumors together with checkpoint inhibitors in clinical trials.

Tue Oct 01 00:00:00 EDT 2019

Carbon ion irradiation enhances the antitumor efficacy of dual immune checkpoint blockade therapy both for local and distant sites in murine osteosarcoma.

Oncotarget

Takahashi Y,Yasui T,Minami K,Tamari K,Hayashi K,Otani K,Seo Y,Isohashi F,Koizumi M,Ogawa K

17-0042 was used in Flow cytometry/Cell sorting to understand the efficacy of combining carbon ion irradiation and dual immune checkpoint blockade therapy to treat local and distant sites in osteosarcoma in mice.

Fri Jan 18 00:00:00 EST 2019

The Sympathetic Nervous System Mitigates CNS Autoimmunity via β2-Adrenergic Receptor Signaling in Immune Cells.

Cell reports

Araujo LP,Maricato JT,Guereschi MG,Takenaka MC,Nascimento VM,de Melo FM,Quintana FJ,Brum PC,Basso AS

17-0042-82 was used in Flow Cytometry to uncover a suppressive role for the SNS in CNS autoimmunity while they identify potential targets for therapeutic intervention.

Tue Sep 17 00:00:00 EDT 2019

Chloroquine modulates inflammatory autoimmune responses through Nurr1 in autoimmune diseases.

Scientific reports

Park TY,Jang Y,Kim W,Shin J,Toh HT,Kim CH,Yoon HS,Leblanc P,Kim KS

17-0042 was used in Flow cytometry/Cell sorting to show the orphan nuclear receptor Nurr1 mediates Foxp3 expression and differentiation of TREG cells leading to symptoms of inflammatory bowel disease.

Tue Oct 29 00:00:00 EDT 2019

Modulation of autoimmune pathogenesis by T cell-triggered inflammatory cell death.

Nature communications

Sasaki K,Himeno A,Nakagawa T,Sasaki Y,Kiyonari H,Iwai K

17-0042-82 was used in Flow Cytometry to show that T cell-mediated killing and sequential autoinflammation are common and crucial for pathogenic diversity during T cell-mediated autoimmune responses.

Wed Aug 28 00:00:00 EDT 2019

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Ziegler PK,Bollrath J,Pallangyo CK,Matsutani T,Canli Ö,De Oliveira T,Diamanti MA,Müller N,Gamrekelashvili J,Putoczki T,Horst D,Mankan AK,Öner MG,Müller S,Müller-Höcker J,Kirchner T,Slotta-Huspenina J,Taketo MM,Reinheckel T,Dröse S,Larner AC,Wels WS,Ernst M,Greten TF,Arkan MC,Korn T,Wirth D,Greten FR

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Jun 28 00:00:00 EDT 2018

T-Cell-Intrinsic Receptor Interacting Protein 2 Regulates Pathogenic T Helper 17 Cell Differentiation.

Immunity

Shimada K,Porritt RA,Markman JL,O'Rourke JG,Wakita D,Noval Rivas M,Ogawa C,Kozhaya L,Martins GA,Unutmaz D,Baloh RH,Crother TR,Chen S,Arditi M

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Nov 20 00:00:00 EST 2018

TCF-1 and HEB cooperate to establish the epigenetic and transcription profiles of CD4⁺CD8⁺ thymocytes.

Nature immunology

Emmanuel AO,Arnovitz S,Haghi L,Mathur PS,Mondal S,Quandt J,Okoreeh MK,Maienschein-Cline M,Khazaie K,Dose M,Gounari F

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Large-Scale Clonal Analysis Resolves Aging of the Mouse Hematopoietic Stem Cell Compartment.

Cell stem cell

Yamamoto R,Wilkinson AC,Ooehara J,Lan X,Lai CY,Nakauchi Y,Pritchard JK,Nakauchi H

17-0042 was used in Flow cytometry/Cell sorting to determine the age-related functional changes to the haematopoietic stem cell compartment.

Thu Apr 05 00:00:00 EDT 2018

Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5.

The Journal of experimental medicine

Buxadé M,Huerga Encabo H,Riera-Borrull M,Quintana-Gallardo L,López-Cotarelo P,Tellechea M,Martínez-Martínez S,Redondo JM,Martín-Caballero J,Flores JM,Bosch E,Rodríguez-Fernández JL,Aramburu J,López-Rodríguez C

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Nov 05 00:00:00 EST 2018

Irg1 expression in myeloid cells prevents immunopathology during M. tuberculosis infection.

The Journal of experimental medicine

Nair S,Huynh JP,Lampropoulou V,Loginicheva E,Esaulova E,Gounder AP,Boon ACM,Schwarzkopf EA,Bradstreet TR,Edelson BT,Artyomov MN,Stallings CL,Diamond MS

17-0042 was used in Flow cytometry/Cell sorting to study the role of Immune responsive gene 1 in the modulation of inflammation during Mycobacterium tuberculosis infection.

Mon Apr 02 00:00:00 EDT 2018

Effector CD4 T cells with progenitor potential mediate chronic intestinal inflammation.

The Journal of experimental medicine

Shin B,Kress RL,Kramer PA,Darley-Usmar VM,Bellis SL,Harrington LE

17-0042 was used in Flow cytometry/Cell sorting to indicate that the chronicity of T cell-mediated inflammation is perpetuated by specific effector CD4 T cells with stem-like properties.

Mon Jul 02 00:00:00 EDT 2018

In Vitro Priming of Adoptively Transferred T Cells with a RORγ Agonist Confers Durable Memory and Stemness In Vivo.

Cancer research

Hu X,Majchrzak K,Liu X,Wyatt MM,Spooner CJ,Moisan J,Zou W,Carter LL,Paulos CM

17-0042 was used in Flow cytometry/Cell sorting to demonstrate that addition of a synthetic, small-molecule RORγ agonist during ex vivo expansion potentiates the anti-tumour activity of human Th17 and Tc17 cells redirected with a chimeric antigen receptor.

Sun Jul 15 00:00:00 EDT 2018

Bryostatin-1 alleviates experimental multiple sclerosis.

Proceedings of the National Academy of Sciences of the United States of America

Kornberg MD,Smith MD,Shirazi HA,Calabresi PA,Snyder SH,Kim PM

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Feb 27 00:00:00 EST 2018

Quantitative Methods for Measuring Repair Rates and Innate-Immune Cell Responses in Wounded Mouse Skin.

Frontiers in immunology

Li Z,Gothard E,Coles MC,Ambler CA

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Mar 27 00:00:00 EDT 2019

Lenalidomide and Programmed Death-1 Blockade Synergistically Enhances the Effects of Dendritic Cell Vaccination in a Model of Murine Myeloma.

Frontiers in immunology

Vo MC,Jung SH,Chu TH,Lee HJ,Lakshmi TJ,Park HS,Kim HJ,Rhee JH,Lee JJ

17-0042 was used in Flow cytometry/Cell sorting to investigate the efficacy of dendritic cell vaccination in combination with lenalidomide and PD-1 blockade in a model of murine myeloma.

Sun Jan 03 00:00:00 EST 2021

Protease-activated receptor-2 signaling through β-arrestin-2 mediates Alternaria alkaline serine protease-induced airway inflammation.

American journal of physiology. Lung cellular and molecular physiology

Yee MC,Nichols HL,Polley D,Saifeddine M,Pal K,Lee K,Wilson EH,Daines MO,Hollenberg MD,Boitano S,DeFea KA

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Intraperitoneal neutrophils activated by KRAS-induced ovarian cancer exert antitumor effects by modulating adaptive immunity.

International journal of oncology

Yoshida M,Taguchi A,Kawana K,Ogishima J,Adachi K,Kawata A,Nakamura H,Sato M,Fujimoto A,Inoue T,Tomio K,Mori M,Nagamatsu T,Arimoto T,Koga K,Hiraike OW,Oda K,Kiyono T,Osuga Y,Fujii T

17-0042 was used in Flow cytometry/Cell sorting to evaluate the role of increased neutrophils in cancer progression.

Mon Oct 01 00:00:00 EDT 2018

Granulocyte-macrophage colony-stimulating factor neuroprotective activities in Alzheimer's disease mice.

Journal of neuroimmunology

Kiyota T,Machhi J,Lu Y,Dyavarshetty B,Nemati M,Yokoyama I,Mosley RL,Gendelman HE

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Jun 15 00:00:00 EDT 2018

Immunological effects of a low-dose cytarabine, aclarubicin and granulocyte-colony stimulating factor priming regimen on a mouse leukemia model.

Oncology letters

Chen J,Yang N,Liu H,Yao H,Wang J,Yang Y,Zhang W

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Protective function of Bu Shen Huo Xue formula on the immunity of B6AF1 mice with experimental autoimmune premature ovarian failure.

Experimental and therapeutic medicine

Wang P,Lu Y,Chen S,Chen Y,Hu C,Zuo Y

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Runx-dependent and silencer-independent repression of a maturation enhancer in the Cd4 gene.

Nature communications

Kojo S,Yasmin N,Muroi S,Tenno M,Taniuchi I

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Sep 05 00:00:00 EDT 2018

Modifying the cancer-immune set point using vaccinia virus expressing re-designed interleukin-2.

Nature communications

Liu Z,Ge Y,Wang H,Ma C,Feist M,Ju S,Guo ZS,Bartlett DL

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Nov 08 00:00:00 EST 2018

Rap1 deficiency-provoked paracrine dysfunction impairs immunosuppressive potency of mesenchymal stem cells in allograft rejection of heart transplantation.

Cell death & disease

Ding Y,Liang X,Zhang Y,Yi L,Shum HC,Chen Q,Chan BP,Fan H,Liu Z,Tergaonkar V,Qi Z,Tse HF,Lian Q

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Mar 07 00:00:00 EST 2018

Inactivation of TRPM7 kinase in mice results in enlarged spleens, reduced T-cell proliferation and diminished store-operated calcium entry.

Scientific reports

Beesetty P,Wieczerzak KB,Gibson JN,Kaitsuka T,Luu CT,Matsushita M,Kozak JA

17-0042 was used in Flow cytometry/Cell sorting to study the role of TRPM7 channel kinase as a novel modulator of blastogenesis, proliferation and store-operated calcium entry.

Wed Feb 14 00:00:00 EST 2018

Fundamental parameters of the developing thymic epithelium in the mouse.

Scientific reports

Hirakawa M,Nagakubo D,Kanzler B,Avilov S,Krauth B,Happe C,Swann JB,Nusser A,Boehm T

17-0042 was used in Flow cytometry/Cell sorting to study the dynamic of thymic epithelial cells and thymocytes during embryogenesis.

Mon Jul 23 00:00:00 EDT 2018

NF1 heterozygosity fosters de novo tumorigenesis but impairs malignant transformation.

Nature communications

Brosseau JP,Liao CP,Wang Y,Ramani V,Vandergriff T,Lee M,Patel A,Ariizumi K,Le LQ

17-0042 was used in Flow cytometry/Cell sorting to study the role of the neurofibromatosis type 1 tumour suppressor gene in de novo tumorigenesis and malignant transformation.

Tue Nov 27 00:00:00 EST 2018

Development of a screening strategy for new modulators of T cell receptor signaling and T cell activation.

Scientific reports

Chen EW,Brzostek J,Gascoigne NRJ,Rybakin V

Tue Jul 03 00:00:00 EDT 2018

Age-specific biological and molecular profiling distinguishes paediatric from adult acute myeloid leukaemias.

Nature communications

Chaudhury S,O'Connor C,Cañete A,Bittencourt-Silvestre J,Sarrou E,Prendergast Á,Choi J,Johnston P,Wells CA,Gibson B,Keeshan K

17-0042 was used in Flow cytometry/Cell sorting to study paediatric acute myeloid leukaemia biology, and provide murine models that offer the potential for developing paediatric specific therapeutic strategies.

Tue Dec 11 00:00:00 EST 2018

CCR8 is expressed by post-positive selection CD4-lineage thymocytes but is dispensable for central tolerance induction.

PloS one

Thyagarajan HM,Lancaster JN,Lira SA,Ehrlich LIR

17-0042 was used in flow-cytometry to investigate whether chemokine receptor CCR8 promotes thymocyte medullary entry and/or rapid motility to induce negative selection.

Tue Jan 22 00:00:00 EST 2019

Periodontitis induced by Porphyromonas gingivalis drives periodontal microbiota dysbiosis and insulin resistance via an impaired adaptive immune response.

Gut

Blasco-Baque V,Garidou L,Pomié C,Escoula Q,Loubieres P,Le Gall-David S,Lemaitre M,Nicolas S,Klopp P,Waget A,Azalbert V,Colom A,Bonnaure-Mallet M,Kemoun P,Serino M,Burcelin R

17-0042 was used in Flow cytometry/Cell sorting to determine the causal mechanism responsible for the enhancement of hyperglycemia and insulin resistance in mice fed a fat-enriched diet.

Mon May 01 00:00:00 EDT 2017

Ubiquitination of tumor suppressor PML regulates prometastatic and immunosuppressive tumor microenvironment.

The Journal of clinical investigation

Wang YT,Chen J,Chang CW,Jen J,Huang TY,Chen CM,Shen R,Liang SY,Cheng IC,Yang SC,Lai WW,Cheng KH,Hsieh TS,Lai MZ,Cheng HC,Wang YC,Chen RH

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

CTCF-Mediated Enhancer-Promoter Interaction Is a Critical Regulator of Cell-to-Cell Variation of Gene Expression.

Molecular cell

Ren G,Jin W,Cui K,Rodrigez J,Hu G,Zhang Z,Larson DR,Zhao K

17-0042 was used in Flow cytometry/Cell sorting to study the role of CTCF in regulating cell-to-cell variation of expression.

Thu Sep 21 00:00:00 EDT 2017

ILC2s regulate adaptive Th2 cell functions via PD-L1 checkpoint control.

The Journal of experimental medicine

Schwartz C,Khan AR,Floudas A,Saunders SP,Hams E,Rodewald HR,McKenzie ANJ,Fallon PG

17-0042 was used in Flow cytometry/Cell sorting to study how group 2 innate lymphoid cells regulate adaptive Th2 cell functions.

Mon Sep 04 00:00:00 EDT 2017

IL-36 Signaling Facilitates Activation of the NLRP3 Inflammasome and IL-23/IL-17 Axis in Renal Inflammation and Fibrosis.

Journal of the American Society of Nephrology : JASN

Chi HH,Hua KF,Lin YC,Chu CL,Hsieh CY,Hsu YJ,Ka SM,Tsai YL,Liu FC,Chen A

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2017

IL-36 Signaling Facilitates Activation of the NLRP3 Inflammasome and IL-23/IL-17 Axis in Renal Inflammation and Fibrosis.

Journal of the American Society of Nephrology : JASN

Chi HH,Hua KF,Lin YC,Chu CL,Hsieh CY,Hsu YJ,Ka SM,Tsai YL,Liu FC,Chen A

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2017

Intestinal Batf3-dependent dendritic cells are required for optimal antiviral T-cell responses in adult and neonatal mice.

Mucosal immunology

Sun T,Rojas OL,Li C,Ward LA,Philpott DJ,Gommerman JL

17-0042 was used in Flow cytometry/Cell sorting to study the dendritic cell subset(s) that provoke antiviral responses within the gut.

Mon May 01 00:00:00 EDT 2017

Subunit vaccine H56/CAF01 induces a population of circulating CD4 T cells that traffic into the Mycobacterium tuberculosis-infected lung.

Mucosal immunology

Woodworth JS,Cohen SB,Moguche AO,Plumlee CR,Agger EM,Urdahl KB,Andersen P

17-0042 was used in Flow cytometry/Cell sorting to elucidate how H56/CAF01 induces a population of circulating CD4 T cells that traffic into the Mycobacterium tuberculosis-infected lung.

Wed Mar 01 00:00:00 EST 2017

The NLRP3 inflammasome contributes to host protection during Sporothrix schenckii infection.

Immunology

Gonçalves AC,Ferreira LS,Manente FA,de Faria CMQG,Polesi MC,de Andrade CR,Zamboni DS,Carlos IZ

17-0042 was used in Flow cytometry/Cell sorting to indicate that the NLRP3 inflammasome contributes to protecion against infection from S. schenckii by linking the innate recognition of S. schenckii to the adaptive immune response.

Thu Jun 01 00:00:00 EDT 2017

Chemokine Receptor Ccr7 Restricts Fatal West Nile Virus Encephalitis.

Journal of virology

Bardina SV,Brown JA,Michlmayr D,Hoffman KW,Sum J,Pletnev AG,Lira SA,Lim JK

17-0042 was used in Flow cytometry/Cell sorting to point to Ccr7 as a critical host defense restriction factor limiting neuroinflammation during acute viral infection.

Mon May 15 00:00:00 EDT 2017

IL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury.

Frontiers in neurology

Gao W,Li F,Zhou Z,Xu X,Wu Y,Zhou S,Yin D,Sun D,Xiong J,Jiang R,Zhang J

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Discrimination of the heterogeneity of bone marrow‑derived dendritic cells.

Molecular medicine reports

Wang J,Dai X,Hsu C,Ming C,He Y,Zhang J,Wei L,Zhou P,Wang CY,Yang J,Gong N

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Immunological effects of vaccines combined with granulocyte colony-stimulating factor on a murine WEHI-3 leukemia model.

Oncology letters

Chen J,Zhang M,Zhou F,Wang J,Niu B,Zhang W

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Effect of follicular helper T cells on the pathogenesis of asthma.

Experimental and therapeutic medicine

Ma X,Sun Z,Zhai P,Yu W,Wang T,Li F,Ding J

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

IL-7-dependent STAT1 activation limits homeostatic CD4+ T cell expansion.

JCI insight

Le Saout C,Luckey MA,Villarino AV,Smith M,Hasley RB,Myers TG,Imamichi H,Park JH,O'Shea JJ,Lane HC,Catalfamo M

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Nov 16 00:00:00 EST 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Batf3-dependent CD8α⁺ Dendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages.

EBioMedicine

Li Y,Liu X,Duan W,Tian H,Zhu G,He H,Yao S,Yi S,Song W,Tang H

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting.

Scientific reports

Atkin-Smith GK,Paone S,Zanker DJ,Duan M,Phan TK,Chen W,Hulett MD,Poon IK

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Jan 06 00:00:00 EST 2017

Radiation enhanced the local and distant anti-tumor efficacy in dual immune checkpoint blockade therapy in osteosarcoma.

PloS one

Takahashi Y,Yasui T,Tamari K,Minami K,Otani K,Isohashi F,Seo Y,Kambe R,Koizumi M,Ogawa K

17-0042 was used in Flow cytometry/Cell sorting to demonstrate that radiation therapy enhances the efficacy of immune checkpoint blockade therapy, thus providing a promising therapeutic option for osteosarcoma.

Mon Jan 08 00:00:00 EST 2018

NAFLD causes selective CD4(+) T lymphocyte loss and promotes hepatocarcinogenesis.

Nature

Ma C,Kesarwala AH,Eggert T,Medina-Echeverz J,Kleiner DE,Jin P,Stroncek DF,Terabe M,Kapoor V,ElGindi M,Han M,Thornton AM,Zhang H,Egger M,Luo J,Felsher DW,McVicar DW,Weber A,Heikenwalder M,Greten TF

17-0042 was used in Flow cytometry/Cell sorting to demonstrate that dysregulation of lipid metabolism in NAFLD causes selective loss of CD4(+) T lymphocytes, promoting hepatocarcinogenesis.

Thu Mar 10 00:00:00 EST 2016

CD69 controls the uptake of L-tryptophan through LAT1-CD98 and AhR-dependent secretion of IL-22 in psoriasis.

Nature immunology

Cibrian D,Saiz ML,de la Fuente H,Sánchez-Díaz R,Moreno-Gonzalo O,Jorge I,Ferrarini A,Vázquez J,Punzón C,Fresno M,Vicente-Manzanares M,Daudén E,Fernández-Salguero PM,Martín P,Sánchez-Madrid F

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Oligodendrocyte death results in immune-mediated CNS demyelination.

Nature neuroscience

Traka M,Podojil JR,McCarthy DP,Miller SD,Popko B

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

STAT3/5-Dependent IL9 Overexpression Contributes to Neoplastic Cell Survival in Mycosis Fungoides.

Clinical cancer research : an official journal of the American Association for Cancer Research

Vieyra-Garcia PA,Wei T,Naym DG,Fredholm S,Fink-Puches R,Cerroni L,Odum N,O'Malley JT,Gniadecki R,Wolf P

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

Lineage tracing of cells involved in atherosclerosis.

Atherosclerosis

Albarrán-Juárez J,Kaur H,Grimm M,Offermanns S,Wettschureck N

17-0042 was used in Flow cytometry/Cell sorting to identify the origin of vascular smooth muscle cells and macrophages within atherosclerosis lesions.

Mon Aug 01 00:00:00 EDT 2016

Mechanisms of Acute Toxicity in NKG2D Chimeric Antigen Receptor T Cell-Treated Mice.

Journal of immunology (Baltimore, Md. : 1950)

Sentman ML,Murad JM,Cook WJ,Wu MR,Reder J,Baumeister SH,Dranoff G,Fanger MW,Sentman CL

17-0042 was used in Flow cytometry/Cell sorting to examine the mechanisms of acute toxicity in NKG2D chimeric antigen receptor T cell-treated mice.

Thu Dec 15 00:00:00 EST 2016

Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model.

Molecular medicine reports

Yue Y,Li P,Song N,Li B,Li Z,Guo Y,Zhang W,Wei MQ,Gai Z,Meng H,Wang J,Qin L

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Sun May 01 00:00:00 EDT 2016

Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells.

Nature communications

Baptista MA,Keszei M,Oliveira M,Sunahara KK,Andersson J,Dahlberg CI,Worth AJ,Liedén A,Kuo IC,Wallin RP,Snapper SB,Eidsmo L,Scheynius A,Karlsson MC,Bouma G,Burns SO,Forsell MN,Thrasher AJ,Nylén S,Westerberg LS

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Jul 18 00:00:00 EDT 2016

The REGγ-proteasome forms a regulatory circuit with IκBɛ and NFκB in experimental colitis.

Nature communications

Xu J,Zhou L,Ji L,Chen F,Fortmann K,Zhang K,Liu Q,Li K,Wang W,Wang H,Xie W,Wang Q,Liu J,Zheng B,Zhang P,Huang S,Shi T,Zhang B,Dang Y,Chen J,O'Malley BW,Moses RE,Wang P,Li L,Xiao J,Hoffmann A,Li X

17-0042 was used in Flow cytometry/Cell sorting to explore a role of REGγ in the regulation of NFκB and the effect of this on the development of bowel inflammation and associated tumour formation.

Mon Feb 22 00:00:00 EST 2016

Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells.

eLife

Allison KA,Sajti E,Collier JG,Gosselin D,Troutman TD,Stone EL,Hedrick SM,Glass CK

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Jul 04 00:00:00 EDT 2016

Normal Development and Function of T Cells in Proline Rich 7 (Prr7) Deficient Mice.

PloS one

Hrdinka M,Sudan K,Just S,Drobek A,Stepanek O,Schlüter D,Reinhold D,Jordan BA,Gintschel P,Schraven B,Kreutz MR

17-0042 was used in Flow cytometry/Cell sorting to show that Prr7 loss does not result in a major immune system defect in mice.

Wed Nov 20 00:00:00 EST 2019

Absence of apolipoprotein E protects mice from cerebral malaria.

Scientific reports

Kassa FA,Van Den Ham K,Rainone A,Fournier S,Boilard E,Olivier M

17-0042 was used in Flow cytometry/Cell sorting to reveal a unique biomarker, apolipoprotein E, for cerebral malaria.

Tue Sep 20 00:00:00 EDT 2016

Sex bias in CNS autoimmune disease mediated by androgen control of autoimmune regulator.

Nature communications

Zhu ML,Bakhru P,Conley B,Nelson JS,Free M,Martin A,Starmer J,Wilson EM,Su MA

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Apr 13 00:00:00 EDT 2016

Irradiation of necrotic cancer cells, employed for pulsing dendritic cells (DCs), potentiates DC vaccine-induced antitumor immunity against high-grade glioma.

Oncoimmunology

Vandenberk L,Garg AD,Verschuere T,Koks C,Belmans J,Beullens M,Agostinis P,De Vleeschouwer S,Van Gool SW

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Exploring the induction of preproinsulin-specific Foxp3(+) CD4(+) Treg cells that inhibit CD8(+) T cell-mediated autoimmune diabetes by DNA vaccination.

Scientific reports

Stifter K,Schuster C,Schlosser M,Boehm BO,Schirmbeck R

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Jul 11 00:00:00 EDT 2016

Control of signaling-mediated clearance of apoptotic cells by the tumor suppressor p53.

Science (New York, N.Y.)

Yoon KW,Byun S,Kwon E,Hwang SY,Chu K,Hiraki M,Jo SH,Weins A,Hakroush S,Cebulla A,Sykes DB,Greka A,Mundel P,Fisher DE,Mandinova A,Lee SW

17-0042 was used in Flow cytometry/Cell sorting to reveal that p53-induced expression of death domain 1α regulates the efficient clearance of dead cells and proper immune tolerance.

Fri Jul 31 00:00:00 EDT 2015

Distinct germinal center selection at local sites shapes memory B cell response to viral escape.

The Journal of experimental medicine

Adachi Y,Onodera T,Yamada Y,Daio R,Tsuiji M,Inoue T,Kobayashi K,Kurosaki T,Ato M,Takahashi Y

17-0042 was used in Immunohistochemistry on frozen tissues to investigate the cellular events dictating the cross-reactive memory B cell responses, showing that distinct germinal center selection at local sites shapes response to viral escape.

Mon Sep 21 00:00:00 EDT 2015

CCR4 promotes medullary entry and thymocyte-dendritic cell interactions required for central tolerance.

The Journal of experimental medicine

Hu Z,Lancaster JN,Sasiponganan C,Ehrlich LI

17-0042 was used in Immunofluorescence to demonstrate that CCR4 promotes medullary entry of the earliest post-positive selection thymocytes, as well as efficient interactions between medullary thymocytes and dendritic cells.

Mon Oct 19 00:00:00 EDT 2015

Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice.

Cardiovascular research

Wan W,Liu Q,Lionakis MS,Marino AP,Anderson SA,Swamydas M,Murphy PM

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

B-cell very late antigen-4 deficiency reduces leukocyte recruitment and susceptibility to central nervous system autoimmunity.

Annals of neurology

Lehmann-Horn K,Sagan SA,Bernard CC,Sobel RA,Zamvil SS

17-0042 was used in Flow cytometry/Cell sorting to report that the selective inhibition of VLA-4 expression on B cells impedes CNS accumulation of B cells, and recruitment of Th17 cells and macrophages.

Fri May 01 00:00:00 EDT 2015

Negative CD4 + TIM-3 signaling confers resistance against cold preservation damage in mouse liver transplantation.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Liu Y,Ji H,Zhang Y,Shen XD,Gao F,Nguyen TT,Shang X,Lee N,Busuttil RW,Kupiec-Weglinski JW

17-0042 was used in Flow cytometry/Cell sorting to explore TIM-3-Gal-9 function in a clinically relevant murine model of hepatic cold storage and orthotopic liver transplantation.

Wed Apr 01 00:00:00 EDT 2015

Single-Dose CpG Immunization Protects Against a Heterosubtypic Challenge and Generates Antigen-Specific Memory T Cells.

Frontiers in immunology

Vogel AJ,Brown DM

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Jul 10 00:00:00 EDT 2015

Premature thymic involution is independent of structural plasticity of the thymic stroma.

European journal of immunology

Franckaert D,Schlenner SM,Heirman N,Gill J,Skogberg G,Ekwall O,Put K,Linterman MA,Dooley J,Liston A

17-0042 was used in Flow cytometry/Cell sorting to investigate thymic involution in FVB/N mice, showing that disruption of the epithelial-endothelial relationship and a progressive loss of pro-T cells precedes involution, and loss of pro-T cells is sufficient to drive premature involution, suggesting that pro-T cells are the main driver of involution.

Fri May 01 00:00:00 EDT 2015

IκBNS regulates murine Th17 differentiation during gut inflammation and infection.

Journal of immunology (Baltimore, Md. : 1950)

Annemann M,Wang Z,Plaza-Sirvent C,Glauben R,Schuster M,Ewald Sander F,Mamareli P,Kühl AA,Siegmund B,Lochner M,Schmitz I

17-0042 was used in Flow cytometry/Cell sorting to study the role of nuclear IκBNS in murine Th17 cell generation upon inflammation and infection.

Sun Mar 15 00:00:00 EDT 2015

Toll-Like Receptor 4 Is an Essential Upstream Regulator of On-Time Parturition and Perinatal Viability in Mice.

Endocrinology

Wahid HH,Dorian CL,Chin PY,Hutchinson MR,Rice KC,Olson DM,Moldenhauer LM,Robertson SA

17-0042 was used in Flow cytometry/Cell sorting to describe TLR4-mediated regulation of the inflammatory response acting to drive uterine activation and control the timing of labour.

Thu Oct 01 00:00:00 EDT 2015

A tumor lysate is an effective vaccine antigen for the stimulation of CD4(+) T-cell function and subsequent induction of antitumor immunity mediated by CD8(+) T cells.

Cancer biology & therapy

Kawahara M,Takaku H

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Sep 12 00:00:00 EDT 2016

Immunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine.

Human vaccines & immunotherapeutics

Teng X,Tian M,Li J,Tan S,Yuan X,Yu Q,Jing Y,Zhang Z,Yue T,Zhou L,Fan X

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Mar 11 00:00:00 EST 2016

Enhanced T-cell activation and differentiation in lymphocytes from transgenic mice expressing ubiquitination-resistant 2KR LAT molecules.

Gene therapy

Rodriguez-Peña AB,Gomez-Rodriguez J,Kortum RL,Palmer DC,Yu Z,Guittard GC,Wohlfert EA,Silver PB,Misplon JA,Sommers CL,Feigenbaum L,Epstein SL,Caspi RR,Belkaid Y,Restifo NP,Samelson LE,Balagopalan L

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

Neonatal microbial colonization in mice promotes prolonged dominance of CD11b(+)Gr-1(+) cells and accelerated establishment of the CD4(+) T cell population in the spleen.

Immunity, inflammation and disease

Kristensen MB,Metzdorff SB,Bergström A,Damlund DS,Fink LN,Licht TR,Frøkiær H

17-0042 was used in Flow cytometry/Cell sorting to assess the microbial influence on postnatal haematopoiesis.

Tue Sep 01 00:00:00 EDT 2015

Recombinant adeno-vaccine expressing enterovirus 71-like particles against hand, foot, and mouth disease.

PLoS neglected tropical diseases

Tsou YL,Lin YW,Shao HY,Yu SL,Wu SR,Lin HY,Liu CC,Huang C,Chong P,Chow YH

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Cysteine cathepsin activity suppresses osteoclastogenesis of myeloid-derived suppressor cells in breast cancer.

Oncotarget

Edgington-Mitchell LE,Rautela J,Duivenvoorden HM,Jayatilleke KM,van der Linden WA,Verdoes M,Bogyo M,Parker BS

17-0042 was used in Flow cytometry/Cell sorting to suggest that cysteine protease inhibition is associated with enhanced osteoclastogenesis of myeloid-derived suppressor cells in breast cancer.

Tue Sep 29 00:00:00 EDT 2015

dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.

Nature communications

Lim S,Kim WJ,Kim YH,Lee S,Koo JH,Lee JA,Yoon H,Kim DH,Park HJ,Kim HM,Lee HG,Yun Kim J,Lee JU,Hun Shin J,Kyun Kim L,Doh J,Kim H,Lee SK,Bothwell ALM,Suh M,Choi JM

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Sep 15 00:00:00 EDT 2015

Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation.

eLife

He L,Zhang Y,Ma G,Tan P,Li Z,Zang S,Wu X,Jing J,Fang S,Zhou L,Wang Y,Huang Y,Hogan PG,Han G,Zhou Y

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2015

Iron prevents the development of experimental cerebral malaria by attenuating CXCR3-mediated T cell chemotaxis.

PloS one

Van Den Ham KM,Shio MT,Rainone A,Fournier S,Krawczyk CM,Olivier M

17-0042 was used in Flow cytometry/Cell sorting to study the effect of iron in attenuating CXCR3-mediated T cell chemotaxis and migration to the brain and therefore inhibiting Experimental Cerebral Malaria pathology.

Thu Jan 21 00:00:00 EST 2016

STAT4 controls GM-CSF production by both Th1 and Th17 cells during EAE.

Journal of neuroinflammation

McWilliams IL,Rajbhandari R,Nozell S,Benveniste E,Harrington LE

17-0042 was used in Flow cytometry/Cell sorting to establish the role of STAT4 in the regulation of cytokine production during experimental autoimmune encephalomyelitis pathogenesis.

Tue Jun 30 00:00:00 EDT 2015

A Multiple Antigenic Peptide Mimicking Peptidoglycan Induced T Cell Responses to Protect Mice from Systemic Infection with Staphylococcus aureus.

PloS one

Wang XY,Huang ZX,Chen YG,Lu X,Zhu P,Wen K,Fu N,Liu BY

17-0042 was used in Flow cytometry/Cell sorting to synthesise a multiple antigenic peptide, MAP27, with the potential to augment the efficacy of vaccines against S. aureus.

Tue May 24 00:00:00 EDT 2016

The miR-126-VEGFR2 axis controls the innate response to pathogen-associated nucleic acids.

Nature immunology

Agudo J,Ruzo A,Tung N,Salmon H,Leboeuf M,Hashimoto D,Becker C,Garrett-Sinha LA,Baccarini A,Merad M,Brown BD

17-0042 was used in Flow cytometry/Cell sorting to identify the miR-126-VEGFR2 axis as an important regulator of the innate response that operates through multiscale control of pDCs.

Wed Jan 01 00:00:00 EST 2014

The tyrosine phosphatase PTPN22 discriminates weak self peptides from strong agonist TCR signals.

Nature immunology

Salmond RJ,Brownlie RJ,Morrison VL,Zamoyska R

17-0042 was used in Flow cytometry/Cell sorting to study how loss-of-function PTPN22 alleles can lead to the population expansion of effector and/or memory T cells and a predisposition to human autoimmunity.

Mon Sep 01 00:00:00 EDT 2014

BMP4 inhibits breast cancer metastasis by blocking myeloid-derived suppressor cell activity.

Cancer research

Cao Y,Slaney CY,Bidwell BN,Parker BS,Johnstone CN,Rautela J,Eckhardt BL,Anderson RL

17-0042 was used in Flow cytometry/Cell sorting to assess the potential for therapies based on the activation of BMP4 signalling for the treatment of breast cancer.

Mon Sep 15 00:00:00 EDT 2014

Type 1 diabetes in NOD mice unaffected by mast cell deficiency.

Diabetes

Gutierrez DA,Fu W,Schonefeldt S,Feyerabend TB,Ortiz-Lopez A,Lampi Y,Liston A,Mathis D,Rodewald HR

17-0042 was used in Flow cytometry/Cell sorting to investigate the role of mast cells in the onset and incidence of type 1 diabetes, showing that mast cell deficiency does not affect diabetes.

Sat Nov 01 00:00:00 EDT 2014

TLR2 engagement on CD4(+) T cells enhances effector functions and protective responses to Mycobacterium tuberculosis.

European journal of immunology

Reba SM,Li Q,Onwuzulike S,Ding X,Karim AF,Hernandez Y,Fulton SA,Harding CV,Lancioni CL,Nagy N,Rodriguez ME,Wearsch PA,Rojas RE

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu May 01 00:00:00 EDT 2014

Bronchial epithelial injury in the context of alloimmunity promotes lymphocytic bronchiolitis through hyaluronan expression.

American journal of physiology. Lung cellular and molecular physiology

Stober VP,Szczesniak C,Childress Q,Heise RL,Bortner C,Hollingsworth JW,Neuringer IP,Palmer SM,Garantziotis S

17-0042 was used in Flow cytometry/Cell sorting to study the effect of sterile bronchial epithelial injuries on alloimmunity.

Sun Jun 01 00:00:00 EDT 2014

Impact of prostate inflammation on lesion development in the POET3(+)Pten(+/-) mouse model of prostate carcinogenesis.

The American journal of pathology

Burcham GN,Cresswell GM,Snyder PW,Chen L,Liu X,Crist SA,Henry MD,Ratliff TL

17-0042 was used in Flow cytometry/Cell sorting to study the link between prostatitis and prostate cancer development using an inducible mouse model of prostatitis.

Mon Dec 01 00:00:00 EST 2014

An essential role for medullary thymic epithelial cells during the intrathymic development of invariant NKT cells.

Journal of immunology (Baltimore, Md. : 1950)

White AJ,Jenkinson WE,Cowan JE,Parnell SM,Bacon A,Jones ND,Jenkinson EJ,Anderson G

17-0042 was used in Flow cytometry/Cell sorting to investigate whether thymus microenvironments are required in iNKT cell development, showing that medullary thymic epithelial cells are essential for intrathymic development of iNKT cells.

Sat Mar 15 00:00:00 EDT 2014

T cell-derived IL-4/IL-13 protects mice against fatal Schistosoma mansoni infection independently of basophils.

Journal of immunology (Baltimore, Md. : 1950)

Schwartz C,Oeser K,Prazeres da Costa C,Layland LE,Voehringer D

17-0042 was used in Flow cytometry/Cell sorting to determine the contribution of cytokines and basophils for protective immunity against S. mansoni egg-induced pathology.

Wed Oct 01 00:00:00 EDT 2014

GM-CSF-licensed CD11b+ lung dendritic cells orchestrate Th2 immunity to Blomia tropicalis.

Journal of immunology (Baltimore, Md. : 1950)

Zhou Q,Ho AW,Schlitzer A,Tang Y,Wong KH,Wong FH,Chua YL,Angeli V,Mortellaro A,Ginhoux F,Kemeny DM

17-0042 was used in Flow cytometry/Cell sorting to identify GM-CSF-licensed CD11b(+) lung DCs as a key component for induction of Th2 responses.

Tue Jul 15 00:00:00 EDT 2014

Enhanced auto-antibody production and Mott cell formation in FcμR-deficient autoimmune mice.

International immunology

Honjo K,Kubagawa Y,Suzuki Y,Takagi M,Ohno H,Bucy RP,Izui S,Kubagawa H

17-0042 was used in Flow cytometry/Cell sorting to determine the impact of Fcmr ablation on autoimmunity.

Mon Dec 01 00:00:00 EST 2014

In TCR-stimulated T-cells, N-ras regulates specific genes and signal transduction pathways.

PloS one

Lynch SJ,Zavadil J,Pellicer A

17-0042 was used in Flow cytometry/Cell sorting to investigate how N-ras and H-ras play distinct roles in immune cells at the level of the transcriptome.

Wed Jan 28 00:00:00 EST 2015

Steroids alone or as adjunctive therapy with doxycycline fail to improve oviduct damage in mice infected with Chlamydia muridarum.

Clinical and vaccine immunology : CVI

Corr TE,Sullivan J,Frazer LC,Andrews CW,O'Connell CM,Darville T

17-0042 was used in Flow cytometry/Cell sorting to test the ability of dexamethasone to inhibit inflammation and prevent oviduct scarring in mice genitally infected with Chlamydia muridarum, showing that it fails to improve oviduct damage.

Sun Jun 01 00:00:00 EDT 2014

Efficacy of an abbreviated induction regimen of amphotericin B deoxycholate for cryptococcal meningoencephalitis: 3 days of therapy is equivalent to 14 days.

mBio

Livermore J,Howard SJ,Sharp AD,Goodwin J,Gregson L,Felton T,Schwartz JA,Walker C,Moser B,Müller W,Harrison TS,Perfect JR,Hope WW

17-0042 was used in Flow cytometry/Cell sorting to investigate the efficacy of conventional and abbreviated regimens of dAmB for cryptococcal meningoencephalitis in murine and rabbit models.

Tue Jan 28 00:00:00 EST 2014

Leukocyte population dynamics and detection of IL-9 as a major cytokine at the mouse fetal-maternal interface.

PloS one

Habbeddine M,Verbeke P,Karaz S,Bobé P,Kanellopoulos-Langevin C

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Jun 10 00:00:00 EDT 2015

The efficacy of tumor debulking surgery is improved by adjuvant immunotherapy using imiquimod and anti-CD40.

BMC cancer

Khong A,Cleaver AL,Fahmi Alatas M,Wylie BC,Connor T,Fisher SA,Broomfield S,Lesterhuis WJ,Currie AJ,Lake RA,Robinson BW

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Wed Dec 17 00:00:00 EST 2014

Experimental mouse models of T cell-dependent inflammatory bowel disease.

Methods in molecular biology (Clifton, N.J.)

Song-Zhao GX,Maloy KJ

17-0042-82 was used in Flow cytometry/Cell sorting to describe two T cell-dependent models of experimental murine IBD to provide reliable and tractable systems for the analyses of the induction and regulation of chronic inflammation in the gut.

Thu Apr 16 00:00:00 EDT 2015

P2X7 receptor modulates inflammatory and functional pulmonary changes induced by silica.

PloS one

Monção-Ribeiro LC,Faffe DS,Santana PT,Vieira FS,da Graça CL,Marques-da-Silva C,Machado MN,Caruso-Neves C,Zin WA,Borojevic R,Takiya CM,Coutinho-Silva R

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2015

mRNA-based vaccines synergize with radiation therapy to eradicate established tumors.

Radiation oncology (London, England)

Fotin-Mleczek M,Zanzinger K,Heidenreich R,Lorenz C,Kowalczyk A,Kallen KJ,Huber SM

17-0042 was used in Flow cytometry/Cell sorting to investigate how mRNA-based vaccines interact with radiation therapy in treating established tumours.

Fri Aug 15 00:00:00 EDT 2014

MHC class II-dependent B cell APC function is required for induction of CNS autoimmunity independent of myelin-specific antibodies.

The Journal of experimental medicine

Molnarfi N,Schulze-Topphoff U,Weber MS,Patarroyo JC,Prod'homme T,Varrin-Doyer M,Shetty A,Linington C,Slavin AJ,Hidalgo J,Jenne DE,Wekerle H,Sobel RA,Bernard CC,Shlomchik MJ,Zamvil SS

17-0042 was used in Flow cytometry/Cell sorting to investigate the contribution of B cells to antigen presentation and pathogenic T cell activation in the multiple sclerosis model experimental autoimmune encephalomyelitis.

Mon Dec 16 00:00:00 EST 2013

The alarmin Mrp8/14 as regulator of the adaptive immune response during allergic contact dermatitis.

The EMBO journal

Petersen B,Wolf M,Austermann J,van Lent P,Foell D,Ahlmann M,Kupas V,Loser K,Sorg C,Roth J,Vogl T

17-0042 was used in Flow cytometry/Cell sorting to study the role of TLR-4 ligand Mrp8/14 in inflammation and adaptive immunity.

Wed Jan 09 00:00:00 EST 2013

A role for LFA-1 in delaying T-lymphocyte egress from lymph nodes.

The EMBO journal

Reichardt P,Patzak I,Jones K,Etemire E,Gunzer M,Hogg N

17-0042 was used in Flow cytometry/Cell sorting to study the role of the leukocyte function-associated antigen-1 (LFA-1) during T cell migration within and away from the lymph nodes.

Wed Mar 20 00:00:00 EDT 2013

Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice.

Cardiovascular research

Wan W,Lionakis MS,Liu Q,Roffê E,Murphy PM

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

Topical application of soluble CD83 induces IDO-mediated immune modulation, increases Foxp3+ T cells, and prolongs allogeneic corneal graft survival.

Journal of immunology (Baltimore, Md. : 1950)

Bock F,Rössner S,Onderka J,Lechmann M,Pallotta MT,Fallarino F,Boon L,Nicolette C,DeBenedette MA,Tcherepanova IY,Grohmann U,Steinkasserer A,Cursiefen C,Zinser E

17-0042 was used in Flow cytometry/Cell sorting to investigate the local immunomodulatory properties of soluble CD83 application.

Thu Aug 15 00:00:00 EDT 2013

Reduced effectiveness of CD4+Foxp3+ regulatory T cells in CD28-deficient NOD.H-2h4 mice leads to increased severity of spontaneous autoimmune thyroiditis.

Journal of immunology (Baltimore, Md. : 1950)

Ellis JS,Hong SH,Zaghouani H,Braley-Mullen H

17-0042 was used in Flow cytometry/Cell sorting to examine the role of Tregs in the development of iodine-accelerated spontaneous autoimmune thyroiditis in mice.

Fri Nov 15 00:00:00 EST 2013

Protective immunity against Toxoplasma gondii induced by DNA immunization with the gene encoding a novel vaccine candidate: calcium-dependent protein kinase 3.

BMC infectious diseases

Zhang NZ,Huang SY,Zhou DH,Chen J,Xu Y,Tian WP,Lu J,Zhu XQ

17-0042 was used in Flow cytometry/Cell sorting to investigate the potentiality of protective immunity from DNA immunization, using the gene encoding calcium-dependent protein kinase 3.

Thu Oct 31 00:00:00 EDT 2013

Nod2 activates NF-kB in CD4+ T cells but its expression is dispensable for T cell-induced colitis.

PloS one

Zanello G,Goethel A,Forster K,Geddes K,Philpott DJ,Croitoru K

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Thu Jul 31 00:00:00 EDT 2014

Characterization of two distinct lymphoproliferative diseases caused by ectopic expression of the Notch ligand DLL4 on T cells.

PloS one

Xiong H,Maraver A,Latkowski JA,Henderson T,Schlessinger K,Ding Y,Shen J,Tadokoro CE,Lafaille JJ

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Mon Feb 23 00:00:00 EST 2015

Toll-like receptor agonists and alpha-galactosylceramide synergistically enhance the production of interferon-gamma in murine splenocytes.

Scientific reports

Ando T,Ito H,Ohtaki H,Seishima M

17-0042 was used in Flow cytometry/Cell sorting to explore the effects of the costimulation of GalCer and TLR agonists on the immune system and cytokine production.

Fri Feb 14 00:00:00 EST 2014

Efficient capture of infected neutrophils by dendritic cells in the skin inhibits the early anti-leishmania response.

PLoS pathogens

Ribeiro-Gomes FL,Peters NC,Debrabant A,Sacks DL

17-0042 was used in Flow cytometry/Cell sorting to study how exploitation of immunosuppressive effects by L.major following rapid uptake by skin neutrophils allows inhibition of acquired resistance.

Wed Feb 01 00:00:00 EST 2012

Myeloid-derived suppressor cells from tumor-bearing mice impair TGF-β-induced differentiation of CD4+CD25+FoxP3+ Tregs from CD4+CD25-FoxP3- T cells.

Journal of leukocyte biology

Centuori SM,Trad M,LaCasse CJ,Alizadeh D,Larmonier CB,Hanke NT,Kartchner J,Janikashvili N,Bonnotte B,Larmonier N,Katsanis E

17-0042 was used in Flow cytometry/Cell sorting to indicate that gr-MDSCs from TB hosts have the unanticipated ability to restrict immunosuppressive Tregs.

Thu Nov 01 00:00:00 EDT 2012

Evaluation of Lassa virus vaccine immunogenicity in a CBA/J-ML29 mouse model.

Vaccine

Goicochea MA,Zapata JC,Bryant J,Davis H,Salvato MS,Lukashevich IS

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2012

SA-4-1BBL costimulation inhibits conversion of conventional CD4+ T cells into CD4+ FoxP3+ T regulatory cells by production of IFN-γ.

PloS one

Madireddi S,Schabowsky RH,Srivastava AK,Sharma RK,Yolcu ES,Shirwan H

17-0042 was used in Flow cytometry/Cell sorting to investigate whether 4-1BBL can prevent the conversion of effector CD4+ cells into induced T regulatory cells in tumour settings.

Tue Feb 19 00:00:00 EST 2013

The stalk domain and the glycosylation status of the activating natural killer cell receptor NKp30 are important for ligand binding.

The Journal of biological chemistry

Hartmann J,Tran TV,Kaudeer J,Oberle K,Herrmann J,Quagliano I,Abel T,Cohnen A,Gatterdam V,Jacobs A,Wollscheid B,Tampé R,Watzl C,Diefenbach A,Koch J

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Sep 07 00:00:00 EDT 2012

Novel murine dendritic cell lines: a powerful auxiliary tool for dendritic cell research.

Frontiers in immunology

Fuertes Marraco SA,Grosjean F,Duval A,Rosa M,Lavanchy C,Ashok D,Haller S,Otten LA,Steiner QG,Descombes P,Luber CA,Meissner F,Mann M,Szeles L,Reith W,Acha-Orbea H

17-0042 was used in Flow cytometry/Cell sorting to report the generation of new murine DC lines, named MutuDC, originating from cultures of splenic CD8α conventional DC (cDC) tumours.

Tue Nov 20 00:00:00 EST 2012

SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice.

PloS one

Berge T,Grønningsæter IH,Lorvik KB,Abrahamsen G,Granum S,Sundvold-Gjerstad V,Corthay A,Bogen B,Spurkland A

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Tue Aug 26 00:00:00 EDT 2014

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using CD4 monoclonal antibody (Product # 17-0042-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

Immunosuppressive effects of streptozotocin-induced diabetes result in absolute lymphopenia and a relative increase of T regulatory cells.

Diabetes

Muller YD,Golshayan D,Ehirchiou D,Wyss JC,Giovannoni L,Meier R,Serre-Beinier V,Puga Yung G,Morel P,Bühler LH,Seebach JD

17-0042 was used in Flow cytometry/Cell sorting to evaluate how STZ-induced diabetes affects adaptive immunity and the consequences thereof on allograft rejection.

Thu Sep 01 00:00:00 EDT 2011

The purinergic G protein-coupled receptor 6 inhibits effector T cell activation in allergic pulmonary inflammation.

Journal of immunology (Baltimore, Md. : 1950)

Giannattasio G,Ohta S,Boyce JR,Xing W,Balestrieri B,Boyce JA

17-0042 was used in Flow cytometry/Cell sorting to reveal the role of P2Y(6) receptors for protecting the lung against exuberant allergen-induced pulmonary inflammation.

Mon Aug 01 00:00:00 EDT 2011

Cutting edge: attrition of Plasmodium-specific memory CD8 T cells results in decreased protection that is rescued by booster immunization.

Journal of immunology (Baltimore, Md. : 1950)

Schmidt NW,Harty JT

17-0042 was used in Flow cytometry/Cell sorting to investigate whether unrelated infections will compromise numbers of Plasmodium-specific memory CD8 T cells, showing that attrition of Plasmodium-specific cells is rescued by booster immunisation.

Fri Apr 01 00:00:00 EDT 2011

Combination treatment of mice with CRx-153 (nortriptyline and desloratadine) decreases the severity of experimental autoimmune encephalomyelitis.

Cellular immunology

Podojil JR,Padval MV,Miller SD

17-0042 was used in Flow cytometry/Cell sorting to determine if treatment of PLP(139-151)-induced R-EAE with a combination of two drugs decreases R-EAE disease.

Fri Oct 14 00:00:00 EDT 2011

Low CD4/CD8 T-cell ratio associated with inflammatory arthropathy in human T-cell leukemia virus type I Tax transgenic mice.

PloS one

Ohsugi T,Kumasaka T

17-0042 was used in Flow cytometry/Cell sorting to identify the primary T-cell subsets involved in the development of arthropathy in Tax transgenic mice.

Fri Apr 01 00:00:00 EDT 2011

One naive T cell, multiple fates in CD8+ T cell differentiation.

The Journal of experimental medicine

Gerlach C,van Heijst JW,Swart E,Sie D,Armstrong N,Kerkhoven RM,Zehn D,Bevan MJ,Schepers K,Schumacher TN

17-0042 was used in Flow cytometry/Cell sorting to assess the potential for development of single naive T cells into different subsets.

Mon Jun 07 00:00:00 EDT 2010

A CD1d-dependent antagonist inhibits the activation of invariant NKT cells and prevents development of allergen-induced airway hyperreactivity.

Journal of immunology (Baltimore, Md. : 1950)

Lombardi V,Stock P,Singh AK,Kerzerho J,Yang W,Sullivan BA,Li X,Shiratsuchi T,Hnatiuk NE,Howell AR,Yu KO,Porcelli SA,Tsuji M,Kronenberg M,Wilson SB,Akbari O

17-0042 was used in Flow cytometry/Cell sorting to test whether iNKT cell-targeted therapy could be used to treat allergen-induced airway disease.

Mon Feb 15 00:00:00 EST 2010

Cutting edge: spontaneous development of IL-17-producing gamma delta T cells in the thymus occurs via a TGF-beta 1-dependent mechanism.

Journal of immunology (Baltimore, Md. : 1950)

Do JS,Fink PJ,Li L,Spolski R,Robinson J,Leonard WJ,Letterio JJ,Min B

17-0042 was used in Flow cytometry/Cell sorting to investigate the mechanisms underlying the generation of gammadelta T cells, showing that it occurs via a TGF-beta 1-dependent mechanism.

Mon Feb 15 00:00:00 EST 2010

T follicular helper cells differentiate from Th2 cells in response to helminth antigens.

The Journal of experimental medicine

Glatman Zaretsky A,Taylor JJ,King IL,Marshall FA,Mohrs M,Pearce EJ

17-0042 was used in Immunohistochemistry on frozen tissues to investigate the relationship between T follicular helper cells and T helper subsets.

Mon May 11 00:00:00 EDT 2009

T follicular helper cells differentiate from Th2 cells in response to helminth antigens.

The Journal of experimental medicine

Glatman Zaretsky A,Taylor JJ,King IL,Marshall FA,Mohrs M,Pearce EJ

17-0042 was used in Immunohistochemistry on frozen tissues to investigate the relationship between T follicular helper cells and T helper subsets.

Mon May 11 00:00:00 EDT 2009

IL-4-producing CD4+ T cells in reactive lymph nodes during helminth infection are T follicular helper cells.

The Journal of experimental medicine

King IL,Mohrs M

17-0042 was used in Immunohistochemistry on frozen tissues to investigate the location of IL-4-producing T cells in the lymph nodes.

Mon May 11 00:00:00 EDT 2009

Dendritic cells matured by inflammation induce CD86-dependent priming of naive CD8+ T cells in the absence of their cognate peptide antigen.

Journal of immunology (Baltimore, Md. : 1950)

Maroof A,Beattie L,Kirby A,Coles M,Kaye PM

17-0042 was used in Flow cytometry/Cell sorting to study how inflammation-matured DCs can induce Ag-independent T cell proliferation.

Tue Dec 01 00:00:00 EST 2009

NKG2A inhibits invariant NKT cell activation in hepatic injury.

Journal of immunology (Baltimore, Md. : 1950)

Kawamura T,Takeda K,Kaneda H,Matsumoto H,Hayakawa Y,Raulet DH,Ikarashi Y,Kronenberg M,Yagita H,Kinoshita K,Abo T,Okumura K,Smyth MJ

17-0042 was used in Flow cytometry/Cell sorting to investigate the role of NK cell receptors in iNKT cell activation, showing that NKG2A-mediated signalling negatively regulated iNKT activation and hepatic injury.

Thu Jan 01 00:00:00 EST 2009

Induction of airway hyperreactivity by IL-25 is dependent on a subset of invariant NKT cells expressing IL-17RB.

Journal of immunology (Baltimore, Md. : 1950)

Stock P,Lombardi V,Kohlrautz V,Akbari O

17-0042 was used in Flow cytometry/Cell sorting to investigate the invariant NKT cell dependent pathway by which IL-25 induces Th2 responses and airway hyperreactivity in mice.

Wed Apr 15 00:00:00 EDT 2009

GITR Blockade Facilitates Treg Mediated Allograft Survival.

Transplantation

Sonawane SB,Kim JI,Lee MK,Lee SH,Duff PE,Moore DJ,Lian MM,Deng S,Choi Y,Yeh H,Caton AJ,Markmann JF

17-0042 was used in Flow cytometry/Cell sorting to study how inflammation and other innate immune signals may activate antigen presenting cells to up-regulate GITR.

Fri Nov 27 00:00:00 EST 2009

Induction of protective immunity by vaccination against Chlamydia trachomatis using the major outer membrane protein adjuvanted with CpG oligodeoxynucleotide coupled to the nontoxic B subunit of cholera toxin.

Vaccine

Cheng C,Bettahi I,Cruz-Fisher MI,Pal S,Jain P,Jia Z,Holmgren J,Harandi AM,de la Maza LM

17-0042 was used in Flow cytometry/Cell sorting to test the efficacy of immunisation with the nMOMP of Chlamydia trachomatis mouse pneumonitis serovar in combination with CpG oligodeoxynucleotide linked to the nontoxic B subunit of cholera toxin to elicit a protective immune response to C. trachomatis.

Mon Oct 19 00:00:00 EDT 2009

Lack of IL-6 during coxsackievirus infection heightens the early immune response resulting in increased severity of chronic autoimmune myocarditis.

PloS one

Poffenberger MC,Straka N,El Warry N,Fang D,Shanina I,Horwitz MS

17-0042 was used in Flow cytometry/Cell sorting to investigate the mechanism by which coxsackievirus infection leads to chronic autoimmune pathology, showing that lack of IL-6 heightens the early immune response.

Thu Jul 09 00:00:00 EDT 2009

Some putative prebiotics increase the severity of Salmonella enterica serovar Typhimurium infection in mice.

BMC microbiology

Petersen A,Heegaard PM,Pedersen AL,Andersen JB,Sørensen RB,Frøkiaer H,Lahtinen SJ,Ouwehand AC,Poulsen M,Licht TR

17-0042 was used in Flow cytometry/Cell sorting to examine whether Salmonella infection could be prevented by administration of dietary carbohydrates with different structures and digestibility profiles.

Mon Nov 30 00:00:00 EST 2009

Autoantibody production in lpr/lpr gld/gld mice reflects accumulation of CD4+ effector cells that are resistant to regulatory T cell activity.

Journal of autoimmunity

Hondowicz BD,Fields ML,Nish SA,Larkin J,Caton AJ,Erikson J

17-0042 was used in Flow cytometry/Cell sorting to show that Treg cells block lpr/lpr gld/gld Th cells from providing help to anti-chromatin B cells in an in vivo transfer system.

Mon Sep 01 00:00:00 EDT 2008

Loss of extracellular superoxide dismutase leads to acute lung damage in the presence of ambient air: a potential mechanism underlying adult respiratory distress syndrome.

The American journal of pathology

Gongora MC,Lob HE,Landmesser U,Guzik TJ,Martin WD,Ozumi K,Wall SM,Wilson DS,Murthy N,Gravanis M,Fukai T,Harrison DG

17-0042 was used in Flow cytometry/Cell sorting to study the role of acute SOD3 reduction in lung injury.

Wed Oct 01 00:00:00 EDT 2008

Sunlight triggers cutaneous lupus through a CSF-1-dependent mechanism in MRL-Fas(lpr) mice.

Journal of immunology (Baltimore, Md. : 1950)

Menke J,Hsu MY,Byrne KT,Lucas JA,Rabacal WA,Croker BP,Zong XH,Stanley ER,Kelley VR

17-0042 was used in Flow cytometry/Cell sorting to investigate the role of UVB in cutaneous lupus erythematosus, showing that UVB triggers it through a CSF-1-dependent, macrophage-mediated mechanism.

Sat Nov 15 00:00:00 EST 2008

Chimeric NKG2D modified T cells inhibit systemic T-cell lymphoma growth in a manner involving multiple cytokines and cytotoxic pathways.

Cancer research

Zhang T,Barber A,Sentman CL

17-0042 was used in Flow cytometry/Cell sorting to investigate the efficiency and mechanisms by which chimeric NKG2D-modified T cells inhibit systemic T-cell lymphoma growth.

Thu Nov 15 00:00:00 EST 2007

IL-10 regulates movement of intestinally derived CD4+ T cells to the liver.

Journal of immunology (Baltimore, Md. : 1950)

Bliss SK,Bliss SP,Beiting DP,Alcaraz A,Appleton JA

Fri Jun 15 00:00:00 EDT 2007

IFN-gamma acts directly on activated CD4+ T cells during mycobacterial infection to promote apoptosis by inducing components of the intracellular apoptosis machinery and by inducing extracellular proapoptotic signals.

Journal of immunology (Baltimore, Md. : 1950)

Li X,McKinstry KK,Swain SL,Dalton DK

17-0042 was used in Flow cytometry/Cell sorting to investigate IFN-γ-mediated apoptosis mechanisms of CD4+ T Cells during mycobacterial infection, which operate through induction of intra- and extracellular apoptosis signals.

Sun Jul 15 00:00:00 EDT 2007

TGFbeta in the context of an inflammatory cytokine milieu supports de novo differentiation of IL-17-producing T cells.

Immunity

Veldhoen M,Hocking RJ,Atkins CJ,Locksley RM,Stockinger B

17-0042 was used in Flow cytometry/Cell sorting to investigate the generation of IL-17-producing T cells from naive CD4 T cells, showing that TGFbeta supports de novo differentiation of IL-17-producing T cells.

Wed Feb 01 00:00:00 EST 2006

A regulatory role for the C5a anaphylatoxin in type 2 immunity in asthma.

The Journal of clinical investigation

Köhl J,Baelder R,Lewkowich IP,Pandey MK,Hawlisch H,Wang L,Best J,Herman NS,Sproles AA,Zwirner J,Whitsett JA,Gerard C,Sfyroera G,Lambris JD,Wills-Karp M

17-0042 was used in Flow cytometry/Cell sorting to investigate the role of complement component 5 in asthma.

Wed Mar 01 00:00:00 EST 2006

Converting IL-15 to a superagonist by binding to soluble IL-15R{alpha}.

Proceedings of the National Academy of Sciences of the United States of America

Rubinstein MP,Kovar M,Purton JF,Cho JH,Boyman O,Surh CD,Sprent J

Tue Jun 13 00:00:00 EDT 2006

In vitro-expanded antigen-specific regulatory T cells suppress autoimmune diabetes.

The Journal of experimental medicine

Tang Q,Henriksen KJ,Bi M,Finger EB,Szot G,Ye J,Masteller EL,McDevitt H,Bonyhadi M,Bluestone JA

17-0042 was used in Flow cytometry/Cell sorting to develop a robust method to expand antigen-specific regulatory T cells from autoimmune-prone, non-obese diabetic mice.

Mon Jun 07 00:00:00 EDT 2004

Ligand-dependent inhibition of CD1d-restricted NKT cell development in mice transgenic for the activating receptor Ly49D.

The Journal of experimental medicine

Voyle RB,Beermann F,Lees RK,Schümann J,Zimmer J,Held W,MacDonald HR

17-0042 was used in Flow cytometry/Cell sorting to investigate whether immunoreceptor-tyrosine activation and inhibition motifs associated with NK receptors augment or attenuate perceived TCR signals.

Mon Apr 07 00:00:00 EDT 2003

Bioinformatics

Protein Aliases: CD4; CD4 antigen p55; cd4a; fCD4; Leu-3; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

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Gene Aliases: Cd4; L3T4; Ly-4

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UniProt ID: (Mouse) P06332

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Entrez Gene ID: (Mouse) 12504

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Function(s)

glycoprotein binding protein binding zinc ion binding coreceptor activity enzyme binding protein kinase binding protein homodimerization activity

Process(es)

cytokine production immune system process induction by virus of host cell-cell fusion immune response cell adhesion cell surface receptor signaling pathway T cell differentiation maintenance of protein location in cell T cell activation T cell selection protein palmitoleylation positive regulation of protein kinase activity positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling regulation of T cell activation positive regulation of T cell activation

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CD4 Monoclonal Antibody (RM4-5), APC, eBioscience™

CD4 Antibody (17-0042-82) in Flow

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17-0042-82

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