Invitrogen

CD4 Monoclonal Antibody (RM4-5), PE-Cyanine5.5, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

97 Published Figures

117 References

View all (605) CD4 antibodies

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CD4 Antibody (35-0042-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD8a FITC (Product # 11-0081-82) and 0.06 µg of Rat IgG2a K Isotype Control PE-Cyanine5-5 (Product # 35-4321-82) (left) or 0.06 µg of Anti-Mouse CD4 PE-Cyanine5-5 (right). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

CD4 Antibody — Staining of mouse splenocytes and bone marrow cells. Right: As expected based on known relative expression patterns, CD4 clone RM4-5 stains a subset of splenocytes and does not stain any bone marrow cells. Details: Balb/c bone marrow cells (left) and splenocytes (middle) were surface stained with CD4 (clone RM4-5) followed by staining with 7-AAD. Viable bone marrow cells in the lymphoid (blue histogram) and myeloid (purple histogram) gates and viable splenocytes (orange histogram) were used for analysis. {RE}

Product Details

35-0042-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.125 µg/test

View 117 publications 117 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PE-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RM4-5

Conjugate

PE-Cyanine5.5 PE-Cyanine5.5 PE-Cyanine5.5

Excitation/Emission Max

564/692 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11218300

Product Specific Information

Description: The RM4-5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of RM4-5 is blocked by GK1.5.

Applications Reported: This RM4-5 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RM4-5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-822-49) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-54) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 695 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Target Information

The CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Impact of broad-spectrum antibiotics on the gut-microbiota-spleen-brain axis.

Brain, behavior, & immunity - health

Wan X,Eguchi A,Sakamoto A,Fujita Y,Yang Y,Qu Y,Hatano M,Mori C,Hashimoto K

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2023

Klebsiella pneumoniae Induces Inflammatory Bowel Disease Through Caspase-11-Mediated IL18 in the Gut Epithelial Cells.

Cellular and molecular gastroenterology and hepatology

Zhang Q,Su X,Zhang C,Chen W,Wang Y,Yang X,Liu D,Zhang Y,Yang R

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Feb 23 00:00:00 EST 2023

Regulatory T cells require IL6 receptor alpha signaling to control skeletal muscle function and regeneration.

Cell metabolism

Becker M,Joseph SS,Garcia-Carrizo F,Tom RZ,Opaleva D,Serr I,Tschöp MH,Schulz TJ,Hofmann SM,Daniel C

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Oct 03 00:00:00 EDT 2023

Calcaratarin D, a labdane diterpenoid, attenuates mouse asthma via modulating alveolar macrophage function.

British journal of pharmacology

Liao W,Foo HYC,Tran TNQ,Chai CLL,Wong WSF

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Palmitoyl transferases act as novel drug targets for pancreatic cancer.

Journal of translational medicine

Lin Z,Lv Z,Liu X,Huang K

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Apr 10 00:00:00 EDT 2023

Prolonged dysbiosis and altered immunity under nutritional intervention in a physiological mouse model of severe acute malnutrition.

iScience

Hidalgo-Villeda F,Million M,Defoort C,Vannier T,Svilar L,Lagier M,Wagner C,Arroyo-Portilla C,Chasson L,Luciani C,Bossi V,Gorvel JP,Lelouard H,Tomas J

35-0042-82 was used in Flow cytometry/Cell sorting to provide a preclinical model of SAM and have identified key markers to target with future interventions during the education of the immune system to improve SAM whole defects.

Fri Jun 16 00:00:00 EDT 2023

SARS-CoV-2 spike protein promotes inflammatory cytokine activation and aggravates rheumatoid arthritis.

Cell communication and signaling : CCS

Lee AR,Woo JS,Lee SY,Lee YS,Jung J,Lee CR,Park SH,Cho ML

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Mar 02 00:00:00 EST 2023

TRAF6 controls T cell homeostasis by maintaining the equilibrium of MALT1 scaffolding and protease functions.

Frontiers in immunology

O'Neill TJ,Gewies A,Seeholzer T,Krappmann D

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2023

Group 2 Innate Lymphoid Cells Protect Mice from Abdominal Aortic Aneurysm Formation via IL5 and Eosinophils.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Zhang Y,Liu T,Deng Z,Fang W,Zhang X,Zhang S,Wang M,Luo S,Meng Z,Liu J,Sukhova GK,Li D,McKenzie ANJ,Libby P,Shi GP,Guo J

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Mar 01 00:00:00 EST 2023

Aryl hydrocarbon receptor activation ameliorates experimental colitis by modulating the tolerogenic dendritic and regulatory T cell formation.

Cell & bioscience

Cui X,Ye Z,Wang D,Yang Y,Jiao C,Ma J,Tang N,Zhang H

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Sat Apr 23 00:00:00 EDT 2022

IL-33 induces thymic involution-associated naive T cell aging and impairs host control of severe infection.

Nature communications

Xu L,Wei C,Chen Y,Wu Y,Shou X,Chen W,Lu D,Sun H,Li W,Yu B,Wang X,Zhang X,Yu Y,Lei Z,Tang R,Zhu J,Li Y,Lu L,Zhou H,Zhou S,Su C,Chen X

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Sat Nov 12 00:00:00 EST 2022

Fringe GlcNAc-transferases differentially extend O-fucose on endogenous NOTCH1 in mouse activated T cells.

The Journal of biological chemistry

Matsumoto K,Kumar V,Varshney S,Nairn AV,Ito A,Pennarubia F,Moremen KW,Stanley P,Haltiwanger RS

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2022

Breast cancer cell-derived extracellular vesicles promote CD8⁺ T cell exhaustion via TGF-β type II receptor signaling.

Nature communications

Xie F,Zhou X,Su P,Li H,Tu Y,Du J,Pan C,Wei X,Zheng M,Jin K,Miao L,Wang C,Meng X,van Dam H,Ten Dijke P,Zhang L,Zhou F

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2022

Niche-specific MHC II and PD-L1 regulate CD4+CD8αα+ intraepithelial lymphocyte differentiation.

The Journal of experimental medicine

Moon S,Park Y,Hyeon S,Kim YM,Kim JH,Kim H,Park S,Lee KJ,Koo BK,Ha SJ,Lee SW

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Apr 05 00:00:00 EDT 2021

Romidepsin (FK228) regulates the expression of the immune checkpoint ligand PD-L1 and suppresses cellular immune functions in colon cancer.

Cancer immunology, immunotherapy : CII

Shi Y,Fu Y,Zhang X,Zhao G,Yao Y,Guo Y,Ma G,Bai S,Li H

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2021

Potential therapeutic effects of interleukin-35 on the differentiation of naïve T cells into Helios⁺Foxp3⁺ Tregs in clinical and experimental acute respiratory distress syndrome.

Molecular immunology

Wang C,Xie K,Li K,Lin S,Xu F

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Feline adipose tissue-derived mesenchymal stem cells pretreated with IFN-γ enhance immunomodulatory effects through the PGE₂ pathway.

Journal of veterinary science

Park SG,An JH,Li Q,Chae HK,Park SM,Lee JH,Ahn JO,Song WJ,Youn HY

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Mar 01 00:00:00 EST 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Intratumoral expression of interleukin 23 variants using oncolytic vaccinia virus elicit potent antitumor effects on multiple tumor models via tumor microenvironment modulation.

Theranostics

Chen L,Chen H,Ye J,Ge Y,Wang H,Dai E,Ren J,Liu W,Ma C,Ju S,Guo ZS,Liu Z,Bartlett DL

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Aug 04 00:00:00 EDT 2021

Antitumor efficacy of a neoantigen cancer vaccine delivered by electroporation is influenced by microbiota composition.

Oncoimmunology

Lione L,Salvatori E,Petrazzuolo A,Massacci A,Maggio R,Confroti A,Compagnone M,Aurisicchio L,Ciliberto G,Palombo F

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Mar 23 00:00:00 EDT 2021

Evaluation of DNA-Launched Virus-Like Particle Vaccines in an Immune Competent Mouse Model of Chikungunya Virus Infection.

Vaccines

Rayner JO,Kim JH,Roberts RW,Wood RR,Fouty B,Solodushko V

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Apr 02 00:00:00 EDT 2021

Gut Microbiota Dysbiosis Induced by Intracerebral Hemorrhage Aggravates Neuroinflammation in Mice.

Frontiers in microbiology

Yu X,Zhou G,Shao B,Zhou H,Xu C,Yan F,Wang L,Chen G,Li J,Fu X

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed May 26 00:00:00 EDT 2021

Near-infrared photoimmunotherapy targeting human-EGFR in a mouse tumor model simulating current and future clinical trials.

EBioMedicine

Okada R,Furusawa A,Vermeer DW,Inagaki F,Wakiyama H,Kato T,Nagaya T,Choyke PL,Spanos WC,Allen CT,Kobayashi H

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Sat May 01 00:00:00 EDT 2021

Activation of Bone Marrow Adaptive Immunity in Type 2 Diabetes: Rescue by Co-stimulation Modulator Abatacept.

Frontiers in immunology

Santopaolo M,Sullivan N,Thomas AC,Alvino VV,Nicholson LB,Gu Y,Spinetti G,Kallikourdis M,Blom A,Madeddu P

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Jul 02 00:00:00 EDT 2021

Engineered macrophages as near-infrared light activated drug vectors for chemo-photodynamic therapy of primary and bone metastatic breast cancer.

Nature communications

Huang Y,Guan Z,Dai X,Shen Y,Wei Q,Ren L,Jiang J,Xiao Z,Jiang Y,Liu D,Huang Z,Xu X,Luo Y,Zhao C

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Histological, behavioural and flow cytometric datasets relating to acute ischaemic stroke in young, aged and ApoE^-/- mice in the presence and absence of immunomodulation with fingolimod.

Data in brief

Diaz Diaz AC,Malone K,Shearer JA,Moore AC,Waeber C

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Ddb1 Is Essential for the Expansion of CD4⁺ Helper T Cells by Regulating Cell Cycle Progression and Cell Death.

Frontiers in immunology

Yang L,Chen W,Li L,Xiao Y,Fan S,Zhang Q,Xia T,Li M,Hong Y,Zhao T,Li Q,Liu WH,Xiao N

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Dec 20 00:00:00 EST 2021

Local Sustained GM-CSF Delivery by Genetically Engineered Encapsulated Cells Enhanced Both Cellular and Humoral SARS-CoV-2 Spike-Specific Immune Response in an Experimental Murine Spike DNA Vaccination Model.

Vaccines

Vernet R,Charrier E,Cosset E,Fièvre S,Tomasello U,Grogg J,Mach N

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon May 10 00:00:00 EDT 2021

Sequencing, cloning, and antigen binding analysis of monoclonal antibodies isolated from single mouse B cells.

STAR protocols

Viant C,Escolano A,Chen ST,Nussenzweig MC

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Jun 18 00:00:00 EDT 2021

Small-molecule antagonist of VLA-4 (GW559090) attenuated neuro-inflammation by targeting Th17 cell trafficking across the blood-retinal barrier in experimental autoimmune uveitis.

Journal of neuroinflammation

Chen YH,Eskandarpour M,Zhang X,Galatowicz G,Greenwood J,Lightman S,Calder V

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Feb 18 00:00:00 EST 2021

Analysis of T cells in mouse lymphoid tissue and blood with flow cytometry.

STAR protocols

Skordos I,Demeyer A,Beyaert R

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Mar 19 00:00:00 EDT 2021

STING activation normalizes the intraperitoneal vascular-immune microenvironment and suppresses peritoneal carcinomatosis of colon cancer.

Journal for immunotherapy of cancer

Lee SJ,Yang H,Kim WR,Lee YS,Lee WS,Kong SJ,Lee HJ,Kim JH,Cheon J,Kang B,Chon HJ,Kim C

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

Construction, characterization, and immunization of nanoparticles that display a diverse array of influenza HA trimers.

PloS one

Cohen AA,Yang Z,Gnanapragasam PNP,Ou S,Dam KA,Wang H,Bjorkman PJ

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Oct 15 00:00:00 EDT 2021

Protein arginine methyltransferase 5 promotes cholesterol biosynthesis-mediated Th17 responses and autoimmunity.

The Journal of clinical investigation

Webb LM,Sengupta S,Edell C,Piedra-Quintero ZL,Amici SA,Miranda JN,Bevins M,Kennemer A,Laliotis G,Tsichlis PN,Guerau-de-Arellano M

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Methnaridine is an orally bioavailable, fast-killing and long-acting antimalarial agent that cures Plasmodium infections in mice.

British journal of pharmacology

Wang W,Yao J,Chen Z,Sun Y,Shi Y,Wei Y,Zhou H,Yu Y,Li S,Duan L

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

DKK2 blockage-mediated immunotherapy enhances anti-angiogenic therapy of Kras mutated colorectal cancer.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Hu J,Wang Z,Chen Z,Li A,Sun J,Zheng M,Wu J,Shen T,Qiao J,Li L,Li B,Wu D,Xiao Q

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Increased Tim-3 expression on TILs during treatment with the Anchored GM-CSF vaccine and anti-PD-1 antibodies is inversely correlated with response in prostate cancer.

Journal of Cancer

Zhang X,Chen H,Li G,Zhou X,Shi Y,Zou F,Chen Y,Gao J,Yang S,Wu S,Long Z

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Sep 30 00:00:00 EDT 2020

Comparison of rapamycin and methylprednisolone for treating inflammatory muscle disease in a murine model of experimental autoimmune myositis.

Experimental and therapeutic medicine

Kang J,Feng D,Yang F,Tian X,Han W,Jia H

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2020

Effects of CD4⁺ T lymphocytes from ovariectomized mice on bone marrow mesenchymal stem cell proliferation and osteogenic differentiation.

Experimental and therapeutic medicine

Shao BY,Wang L,Yu Y,Chen L,Gan N,Huang WM

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

The lysophospholipase D enzyme Gdpd3 is required to maintain chronic myelogenous leukaemia stem cells.

Nature communications

Naka K,Ochiai R,Matsubara E,Kondo C,Yang KM,Hoshii T,Araki M,Araki K,Sotomaru Y,Sasaki K,Mitani K,Kim DW,Ooshima A,Kim SJ

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2020

Oncolytic vaccinia virus delivering tethered IL-12 enhances antitumor effects with improved safety.

Journal for immunotherapy of cancer

Ge Y,Wang H,Ren J,Liu W,Chen L,Chen H,Ye J,Dai E,Ma C,Ju S,Guo ZS,Liu Z,Bartlett DL

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Sun Mar 01 00:00:00 EST 2020

A novel antibody targeting TIM-3 resulting in receptor internalization for cancer immunotherapy.

Antibody therapeutics

Kuang Z,Li L,Zhang P,Chen B,Wu M,Ni H,Yi S,Zou J,Liu J

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2020

Mechanisms of interleukin 4 mediated increase in efficacy of vaccines against opioid use disorders.

NPJ vaccines

Crouse B,Robinson C,Huseby Kelcher A,Laudenbach M,Abrahante JE,Pravetoni M

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Oct 29 00:00:00 EDT 2020

CD226 Deletion Reduces Type 1 Diabetes in the NOD Mouse by Impairing Thymocyte Development and Peripheral T Cell Activation.

Frontiers in immunology

Shapiro MR,Yeh WI,Longfield JR,Gallagher J,Infante CM,Wellford S,Posgai AL,Atkinson MA,Campbell-Thompson M,Lieberman SM,Serreze DV,Geurts AM,Chen YG,Brusko TM

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed May 26 00:00:00 EDT 2021

Distinct actions of the fermented beverage kefir on host behaviour, immunity and microbiome gut-brain modules in the mouse.

Microbiome

van de Wouw M,Walsh AM,Crispie F,van Leuven L,Lyte JM,Boehme M,Clarke G,Dinan TG,Cotter PD,Cryan JF

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon May 18 00:00:00 EDT 2020

Senolytics prevent mt-DNA-induced inflammation and promote the survival of aged organs following transplantation.

Nature communications

Iske J,Seyda M,Heinbokel T,Maenosono R,Minami K,Nian Y,Quante M,Falk CS,Azuma H,Martin F,Passos JF,Niemann CU,Tchkonia T,Kirkland JL,Elkhal A,Tullius SG

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Aug 27 00:00:00 EDT 2020

Loss of testosterone impairs anti-tumor neutrophil function.

Nature communications

Markman JL,Porritt RA,Wakita D,Lane ME,Martinon D,Noval Rivas M,Luu M,Posadas EM,Crother TR,Arditi M

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Mar 31 00:00:00 EDT 2020

A Commercial Probiotic Induces Tolerogenic and Reduces Pathogenic Responses in Experimental Autoimmune Encephalomyelitis.

Cells

Calvo-Barreiro L,Eixarch H,Ponce-Alonso M,Castillo M,Lebrón-Galán R,Mestre L,Guaza C,Clemente D,Del Campo R,Montalban X,Espejo C

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Apr 07 00:00:00 EDT 2020

Axenic Caenorhabditis elegans antigen protects against development of type-1 diabetes in NOD mice.

Journal of translational autoimmunity

Jackson-Thompson BM,Torrero M,Mitre BK,Long J,Packiam M,Mitre E

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Harnessing Hematopoietic Stem Cell Low Intracellular Calcium Improves Their Maintenance In Vitro.

Cell stem cell

Luchsinger LL,Strikoudis A,Danzl NM,Bush EC,Finlayson MO,Satwani P,Sykes M,Yazawa M,Snoeck HW

35-0042 was used in Flow cytometry/Cell sorting to reveal a physiological feature of HSCs that can be harnessed to improve their maintenance in vitro.

Thu Aug 01 00:00:00 EDT 2019

Clonal copy-number mosaicism in autoreactive T lymphocytes in diabetic NOD mice.

Genome research

Alriyami M,Marchand L,Li Q,Du X,Olivier M,Polychronakos C

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Sun Dec 01 00:00:00 EST 2019

A unique mutator phenotype reveals complementary oncogenic lesions leading to acute leukemia.

JCI insight

Yin M,Baslan T,Walker RL,Zhu YJ,Freeland A,Matsukawa T,Sridharan S,Nussenzweig A,Pruitt SC,Lowe SW,Meltzer PS,Aplan PD

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Dec 05 00:00:00 EST 2019

Mutation of an L-Type Calcium Channel Gene Leads to T Lymphocyte Dysfunction.

Frontiers in immunology

Fenninger F,Han J,Stanwood SR,Nohara LL,Arora H,Choi KB,Munro L,Pfeifer CG,Shanina I,Horwitz MS,Jefferies WA

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Oct 29 00:00:00 EDT 2020

Systemic inhibition of tissue-nonspecific alkaline phosphatase alters the brain-immune axis in experimental sepsis.

Scientific reports

Brichacek AL,Benkovic SA,Chakraborty S,Nwafor DC,Wang W,Jun S,Dakhlallah D,Geldenhuys WJ,Pinkerton AB,Millán JL,Brown CM

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Dec 11 00:00:00 EST 2019

Chloroquine modulates inflammatory autoimmune responses through Nurr1 in autoimmune diseases.

Scientific reports

Park TY,Jang Y,Kim W,Shin J,Toh HT,Kim CH,Yoon HS,Leblanc P,Kim KS

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Oct 29 00:00:00 EDT 2019

Ufbp1 promotes plasma cell development and ER expansion by modulating distinct branches of UPR.

Nature communications

Zhu H,Bhatt B,Sivaprakasam S,Cai Y,Liu S,Kodeboyina SK,Patel N,Savage NM,Sharma A,Kaufman RJ,Li H,Singh N

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Mar 06 00:00:00 EST 2019

Mitophagy in Intestinal Epithelial Cells Triggers Adaptive Immunity during Tumorigenesis.

Cell

Ziegler PK,Bollrath J,Pallangyo CK,Matsutani T,Canli Ö,De Oliveira T,Diamanti MA,Müller N,Gamrekelashvili J,Putoczki T,Horst D,Mankan AK,Öner MG,Müller S,Müller-Höcker J,Kirchner T,Slotta-Huspenina J,Taketo MM,Reinheckel T,Dröse S,Larner AC,Wels WS,Ernst M,Greten TF,Arkan MC,Korn T,Wirth D,Greten FR

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Jun 28 00:00:00 EDT 2018

T-Cell-Intrinsic Receptor Interacting Protein 2 Regulates Pathogenic T Helper 17 Cell Differentiation.

Immunity

Shimada K,Porritt RA,Markman JL,O'Rourke JG,Wakita D,Noval Rivas M,Ogawa C,Kozhaya L,Martins GA,Unutmaz D,Baloh RH,Crother TR,Chen S,Arditi M

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Nov 20 00:00:00 EST 2018

TCF-1 and HEB cooperate to establish the epigenetic and transcription profiles of CD4⁺CD8⁺ thymocytes.

Nature immunology

Emmanuel AO,Arnovitz S,Haghi L,Mathur PS,Mondal S,Quandt J,Okoreeh MK,Maienschein-Cline M,Khazaie K,Dose M,Gounari F

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Macrophage-specific MHCII expression is regulated by a remote Ciita enhancer controlled by NFAT5.

The Journal of experimental medicine

Buxadé M,Huerga Encabo H,Riera-Borrull M,Quintana-Gallardo L,López-Cotarelo P,Tellechea M,Martínez-Martínez S,Redondo JM,Martín-Caballero J,Flores JM,Bosch E,Rodríguez-Fernández JL,Aramburu J,López-Rodríguez C

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Nov 05 00:00:00 EST 2018

Effector CD4 T cells with progenitor potential mediate chronic intestinal inflammation.

The Journal of experimental medicine

Shin B,Kress RL,Kramer PA,Darley-Usmar VM,Bellis SL,Harrington LE

35-0042 was used in Flow cytometry/Cell sorting to indicate that the chronicity of T cell-mediated inflammation is perpetuated by specific effector CD4 T cells with stem-like properties.

Mon Jul 02 00:00:00 EDT 2018

Bryostatin-1 alleviates experimental multiple sclerosis.

Proceedings of the National Academy of Sciences of the United States of America

Kornberg MD,Smith MD,Shirazi HA,Calabresi PA,Snyder SH,Kim PM

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Feb 27 00:00:00 EST 2018

Quantitative Methods for Measuring Repair Rates and Innate-Immune Cell Responses in Wounded Mouse Skin.

Frontiers in immunology

Li Z,Gothard E,Coles MC,Ambler CA

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Mar 27 00:00:00 EDT 2019

Protease-activated receptor-2 signaling through β-arrestin-2 mediates Alternaria alkaline serine protease-induced airway inflammation.

American journal of physiology. Lung cellular and molecular physiology

Yee MC,Nichols HL,Polley D,Saifeddine M,Pal K,Lee K,Wilson EH,Daines MO,Hollenberg MD,Boitano S,DeFea KA

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Sat Dec 01 00:00:00 EST 2018

Granulocyte-macrophage colony-stimulating factor neuroprotective activities in Alzheimer's disease mice.

Journal of neuroimmunology

Kiyota T,Machhi J,Lu Y,Dyavarshetty B,Nemati M,Yokoyama I,Mosley RL,Gendelman HE

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Jun 15 00:00:00 EDT 2018

Immunological effects of a low-dose cytarabine, aclarubicin and granulocyte-colony stimulating factor priming regimen on a mouse leukemia model.

Oncology letters

Chen J,Yang N,Liu H,Yao H,Wang J,Yang Y,Zhang W

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Sat Sep 01 00:00:00 EDT 2018

Protective function of Bu Shen Huo Xue formula on the immunity of B6AF1 mice with experimental autoimmune premature ovarian failure.

Experimental and therapeutic medicine

Wang P,Lu Y,Chen S,Chen Y,Hu C,Zuo Y

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2018

Runx-dependent and silencer-independent repression of a maturation enhancer in the Cd4 gene.

Nature communications

Kojo S,Yasmin N,Muroi S,Tenno M,Taniuchi I

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Sep 05 00:00:00 EDT 2018

Modifying the cancer-immune set point using vaccinia virus expressing re-designed interleukin-2.

Nature communications

Liu Z,Ge Y,Wang H,Ma C,Feist M,Ju S,Guo ZS,Bartlett DL

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Nov 08 00:00:00 EST 2018

Rap1 deficiency-provoked paracrine dysfunction impairs immunosuppressive potency of mesenchymal stem cells in allograft rejection of heart transplantation.

Cell death & disease

Ding Y,Liang X,Zhang Y,Yi L,Shum HC,Chen Q,Chan BP,Fan H,Liu Z,Tergaonkar V,Qi Z,Tse HF,Lian Q

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Mar 07 00:00:00 EST 2018

Ubiquitination of tumor suppressor PML regulates prometastatic and immunosuppressive tumor microenvironment.

The Journal of clinical investigation

Wang YT,Chen J,Chang CW,Jen J,Huang TY,Chen CM,Shen R,Liang SY,Cheng IC,Yang SC,Lai WW,Cheng KH,Hsieh TS,Lai MZ,Cheng HC,Wang YC,Chen RH

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Excessive expression of miR-27 impairs Treg-mediated immunological tolerance.

The Journal of clinical investigation

Cruz LO,Hashemifar SS,Wu CJ,Cho S,Nguyen DT,Lin LL,Khan AA,Lu LF

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Feb 01 00:00:00 EST 2017

IL-36 Signaling Facilitates Activation of the NLRP3 Inflammasome and IL-23/IL-17 Axis in Renal Inflammation and Fibrosis.

Journal of the American Society of Nephrology : JASN

Chi HH,Hua KF,Lin YC,Chu CL,Hsieh CY,Hsu YJ,Ka SM,Tsai YL,Liu FC,Chen A

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Sat Jul 01 00:00:00 EDT 2017

IL-2/Anti-IL-2 Complex Attenuates Inflammation and BBB Disruption in Mice Subjected to Traumatic Brain Injury.

Frontiers in neurology

Gao W,Li F,Zhou Z,Xu X,Wu Y,Zhou S,Yin D,Sun D,Xiong J,Jiang R,Zhang J

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Discrimination of the heterogeneity of bone marrow‑derived dendritic cells.

Molecular medicine reports

Wang J,Dai X,Hsu C,Ming C,He Y,Zhang J,Wei L,Zhou P,Wang CY,Yang J,Gong N

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Nov 01 00:00:00 EDT 2017

Immunological effects of vaccines combined with granulocyte colony-stimulating factor on a murine WEHI-3 leukemia model.

Oncology letters

Chen J,Zhang M,Zhou F,Wang J,Niu B,Zhang W

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Effect of follicular helper T cells on the pathogenesis of asthma.

Experimental and therapeutic medicine

Ma X,Sun Z,Zhai P,Yu W,Wang T,Li F,Ding J

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

Batf3-dependent CD8α⁺ Dendritic Cells Aggravates Atherosclerosis via Th1 Cell Induction and Enhanced CCL5 Expression in Plaque Macrophages.

EBioMedicine

Li Y,Liu X,Duan W,Tian H,Zhu G,He H,Yao S,Yi S,Song W,Tang H

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Isolation of cell type-specific apoptotic bodies by fluorescence-activated cell sorting.

Scientific reports

Atkin-Smith GK,Paone S,Zanker DJ,Duan M,Phan TK,Chen W,Hulett MD,Poon IK

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Jan 06 00:00:00 EST 2017

IL-7-dependent STAT1 activation limits homeostatic CD4+ T cell expansion.

JCI insight

Le Saout C,Luckey MA,Villarino AV,Smith M,Hasley RB,Myers TG,Imamichi H,Park JH,O'Shea JJ,Lane HC,Catalfamo M

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Nov 16 00:00:00 EST 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Extrafollicular CD4⁺ T-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.

Nature communications

Deng R,Hurtz C,Song Q,Yue C,Xiao G,Yu H,Wu X,Muschen M,Forman S,Martin PJ,Zeng D

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Oct 17 00:00:00 EDT 2017

Suppressive IL-17A⁺Foxp3⁺ and ex-Th17 IL-17A^negFoxp3⁺ T_reg cells are a source of tumour-associated T_reg cells.

Nature communications

Downs-Canner S,Berkey S,Delgoffe GM,Edwards RP,Curiel T,Odunsi K,Bartlett DL,Obermajer N

35-0042 was used in Flow cytometry/Cell sorting to demonstrate the tumour-associated conversion of Th17 cells to Treg cells and identify transdifferentiation-associated markers.

Tue Mar 14 00:00:00 EDT 2017

CD69 controls the uptake of L-tryptophan through LAT1-CD98 and AhR-dependent secretion of IL-22 in psoriasis.

Nature immunology

Cibrian D,Saiz ML,de la Fuente H,Sánchez-Díaz R,Moreno-Gonzalo O,Jorge I,Ferrarini A,Vázquez J,Punzón C,Fresno M,Vicente-Manzanares M,Daudén E,Fernández-Salguero PM,Martín P,Sánchez-Madrid F

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Oligodendrocyte death results in immune-mediated CNS demyelination.

Nature neuroscience

Traka M,Podojil JR,McCarthy DP,Miller SD,Popko B

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2016

STAT3/5-Dependent IL9 Overexpression Contributes to Neoplastic Cell Survival in Mycosis Fungoides.

Clinical cancer research : an official journal of the American Association for Cancer Research

Vieyra-Garcia PA,Wei T,Naym DG,Fredholm S,Fink-Puches R,Cerroni L,Odum N,O'Malley JT,Gniadecki R,Wolf P

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2016

Genomic and immunologic factors associated with viral pathogenesis in a lethal EV71 infected neonatal mouse model.

Molecular medicine reports

Yue Y,Li P,Song N,Li B,Li Z,Guo Y,Zhang W,Wei MQ,Gai Z,Meng H,Wang J,Qin L

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Sun May 01 00:00:00 EDT 2016

Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells.

Nature communications

Baptista MA,Keszei M,Oliveira M,Sunahara KK,Andersson J,Dahlberg CI,Worth AJ,Liedén A,Kuo IC,Wallin RP,Snapper SB,Eidsmo L,Scheynius A,Karlsson MC,Bouma G,Burns SO,Forsell MN,Thrasher AJ,Nylén S,Westerberg LS

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Jul 18 00:00:00 EDT 2016

Affinity and dose of TCR engagement yield proportional enhancer and gene activity in CD4+ T cells.

eLife

Allison KA,Sajti E,Collier JG,Gosselin D,Troutman TD,Stone EL,Hedrick SM,Glass CK

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Jul 04 00:00:00 EDT 2016

Exploring the induction of preproinsulin-specific Foxp3(+) CD4(+) Treg cells that inhibit CD8(+) T cell-mediated autoimmune diabetes by DNA vaccination.

Scientific reports

Stifter K,Schuster C,Schlosser M,Boehm BO,Schirmbeck R

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Jul 11 00:00:00 EDT 2016

Sex bias in CNS autoimmune disease mediated by androgen control of autoimmune regulator.

Nature communications

Zhu ML,Bakhru P,Conley B,Nelson JS,Free M,Martin A,Starmer J,Wilson EM,Su MA

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Apr 13 00:00:00 EDT 2016

Irradiation of necrotic cancer cells, employed for pulsing dendritic cells (DCs), potentiates DC vaccine-induced antitumor immunity against high-grade glioma.

Oncoimmunology

Vandenberk L,Garg AD,Verschuere T,Koks C,Belmans J,Beullens M,Agostinis P,De Vleeschouwer S,Van Gool SW

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Atypical chemokine receptor 1 deficiency reduces atherogenesis in ApoE-knockout mice.

Cardiovascular research

Wan W,Liu Q,Lionakis MS,Marino AP,Anderson SA,Swamydas M,Murphy PM

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Jun 01 00:00:00 EDT 2015

Single-Dose CpG Immunization Protects Against a Heterosubtypic Challenge and Generates Antigen-Specific Memory T Cells.

Frontiers in immunology

Vogel AJ,Brown DM

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Jul 10 00:00:00 EDT 2015

IκBNS regulates murine Th17 differentiation during gut inflammation and infection.

Journal of immunology (Baltimore, Md. : 1950)

Annemann M,Wang Z,Plaza-Sirvent C,Glauben R,Schuster M,Ewald Sander F,Mamareli P,Kühl AA,Siegmund B,Lochner M,Schmitz I

35-0042 was used in Flow cytometry/Cell sorting to study the role of nuclear IκBNS in murine Th17 cell generation upon inflammation and infection.

Sun Mar 15 00:00:00 EDT 2015

A tumor lysate is an effective vaccine antigen for the stimulation of CD4(+) T-cell function and subsequent induction of antitumor immunity mediated by CD8(+) T cells.

Cancer biology & therapy

Kawahara M,Takaku H

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Sep 12 00:00:00 EDT 2016

Immunogenicity and protective efficacy of DMT liposome-adjuvanted tuberculosis subunit CTT3H vaccine.

Human vaccines & immunotherapeutics

Teng X,Tian M,Li J,Tan S,Yuan X,Yu Q,Jing Y,Zhang Z,Yue T,Zhou L,Fan X

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Mar 11 00:00:00 EST 2016

Enhanced T-cell activation and differentiation in lymphocytes from transgenic mice expressing ubiquitination-resistant 2KR LAT molecules.

Gene therapy

Rodriguez-Peña AB,Gomez-Rodriguez J,Kortum RL,Palmer DC,Yu Z,Guittard GC,Wohlfert EA,Silver PB,Misplon JA,Sommers CL,Feigenbaum L,Epstein SL,Caspi RR,Belkaid Y,Restifo NP,Samelson LE,Balagopalan L

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2015

Recombinant adeno-vaccine expressing enterovirus 71-like particles against hand, foot, and mouth disease.

PLoS neglected tropical diseases

Tsou YL,Lin YW,Shao HY,Yu SL,Wu SR,Lin HY,Liu CC,Huang C,Chong P,Chow YH

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2015

Regulation of NKT cell-mediated immune responses to tumours and liver inflammation by mitochondrial PGAM5-Drp1 signalling.

Nature communications

Kang YJ,Bang BR,Han KH,Hong L,Shim EJ,Ma J,Lerner RA,Otsuka M

35-0042 was used in Flow cytometry/Cell sorting to determine the involvement of RIPK3 and RIPK3-PGAM5-Drp1/NFAT signalling in NKT cell-mediated immune responses.

Fri Sep 18 00:00:00 EDT 2015

dNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis.

Nature communications

Lim S,Kim WJ,Kim YH,Lee S,Koo JH,Lee JA,Yoon H,Kim DH,Park HJ,Kim HM,Lee HG,Yun Kim J,Lee JU,Hun Shin J,Kyun Kim L,Doh J,Kim H,Lee SK,Bothwell ALM,Suh M,Choi JM

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Sep 15 00:00:00 EDT 2015

Near-infrared photoactivatable control of Ca(2+) signaling and optogenetic immunomodulation.

eLife

He L,Zhang Y,Ma G,Tan P,Li Z,Zang S,Wu X,Jing J,Fang S,Zhou L,Wang Y,Huang Y,Hogan PG,Han G,Zhou Y

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Dec 08 00:00:00 EST 2015

TLR2 engagement on CD4(+) T cells enhances effector functions and protective responses to Mycobacterium tuberculosis.

European journal of immunology

Reba SM,Li Q,Onwuzulike S,Ding X,Karim AF,Hernandez Y,Fulton SA,Harding CV,Lancioni CL,Nagy N,Rodriguez ME,Wearsch PA,Rojas RE

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu May 01 00:00:00 EDT 2014

T cell-derived IL-4/IL-13 protects mice against fatal Schistosoma mansoni infection independently of basophils.

Journal of immunology (Baltimore, Md. : 1950)

Schwartz C,Oeser K,Prazeres da Costa C,Layland LE,Voehringer D

35-0042 was used in Flow cytometry/Cell sorting to determine the contribution of cytokines and basophils for protective immunity against S. mansoni egg-induced pathology.

Wed Oct 01 00:00:00 EDT 2014

Leukocyte population dynamics and detection of IL-9 as a major cytokine at the mouse fetal-maternal interface.

PloS one

Habbeddine M,Verbeke P,Karaz S,Bobé P,Kanellopoulos-Langevin C

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Jun 10 00:00:00 EDT 2015

The efficacy of tumor debulking surgery is improved by adjuvant immunotherapy using imiquimod and anti-CD40.

BMC cancer

Khong A,Cleaver AL,Fahmi Alatas M,Wylie BC,Connor T,Fisher SA,Broomfield S,Lesterhuis WJ,Currie AJ,Lake RA,Robinson BW

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Wed Dec 17 00:00:00 EST 2014

P2X7 receptor modulates inflammatory and functional pulmonary changes induced by silica.

PloS one

Monção-Ribeiro LC,Faffe DS,Santana PT,Vieira FS,da Graça CL,Marques-da-Silva C,Machado MN,Caruso-Neves C,Zin WA,Borojevic R,Takiya CM,Coutinho-Silva R

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Sep 17 00:00:00 EDT 2015

Genetic deletion of chemokine receptor Ccr7 exacerbates atherogenesis in ApoE-deficient mice.

Cardiovascular research

Wan W,Lionakis MS,Liu Q,Roffê E,Murphy PM

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

Intrinsic T- and B-cell defects impair T-cell-dependent antibody responses in mice lacking the actin-bundling protein L-plastin.

European journal of immunology

Todd EM,Deady LE,Morley SC

35-0042 was used in Flow cytometry/Cell sorting to study the role of L-plastin in the modulation of both T- and B-cell immune responses.

Mon Jul 01 00:00:00 EDT 2013

Nod2 activates NF-kB in CD4+ T cells but its expression is dispensable for T cell-induced colitis.

PloS one

Zanello G,Goethel A,Forster K,Geddes K,Philpott DJ,Croitoru K

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Thu Jul 31 00:00:00 EDT 2014

Characterization of two distinct lymphoproliferative diseases caused by ectopic expression of the Notch ligand DLL4 on T cells.

PloS one

Xiong H,Maraver A,Latkowski JA,Henderson T,Schlessinger K,Ding Y,Shen J,Tadokoro CE,Lafaille JJ

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Mon Feb 23 00:00:00 EST 2015

Evaluation of Lassa virus vaccine immunogenicity in a CBA/J-ML29 mouse model.

Vaccine

Goicochea MA,Zapata JC,Bryant J,Davis H,Salvato MS,Lukashevich IS

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2012

The stalk domain and the glycosylation status of the activating natural killer cell receptor NKp30 are important for ligand binding.

The Journal of biological chemistry

Hartmann J,Tran TV,Kaudeer J,Oberle K,Herrmann J,Quagliano I,Abel T,Cohnen A,Gatterdam V,Jacobs A,Wollscheid B,Tampé R,Watzl C,Diefenbach A,Koch J

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Sep 07 00:00:00 EDT 2012

SH2D2A modulates T cell mediated protection to a B cell derived tumor in transgenic mice.

PloS one

Berge T,Grønningsæter IH,Lorvik KB,Abrahamsen G,Granum S,Sundvold-Gjerstad V,Corthay A,Bogen B,Spurkland A

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Tue Aug 26 00:00:00 EDT 2014

Nuclear export of the NF-κB inhibitor IκBα is required for proper B cell and secondary lymphoid tissue formation.

Immunity

Wuerzberger-Davis SM,Chen Y,Yang DT,Kearns JD,Bates PW,Lynch C,Ladell NC,Yu M,Podd A,Zeng H,Huang TT,Wen R,Hoffmann A,Wang D,Miyamoto S

Published figure using CD4 monoclonal antibody (Product # 35-0042-82) in Flow Cytometry

Fri Feb 25 00:00:00 EST 2011

Gamma interferon produced by antigen-specific CD4+ T cells regulates the mucosal immune responses to Citrobacter rodentium infection.

Infection and immunity

Shiomi H,Masuda A,Nishiumi S,Nishida M,Takagawa T,Shiomi Y,Kutsumi H,Blumberg RS,Azuma T,Yoshida M

35-0042 was used in Flow cytometry/Cell sorting to show that the IFN-gamma produced by antigen-specific CD4(+) T cells plays an important role in the defence against C. rodentium.

Tue Jun 01 00:00:00 EDT 2010

In vivo expansion of T reg cells with IL-2-mAb complexes: induction of resistance to EAE and long-term acceptance of islet allografts without immunosuppression.

The Journal of experimental medicine

Webster KE,Walters S,Kohler RE,Mrkvan T,Boyman O,Surh CD,Grey ST,Sprent J

35-0042 was used in Flow cytometry/Cell sorting to study the role of T reg cells in the induction of resistance to EAE and long term acceptance of islet allografts.

Mon Apr 13 00:00:00 EDT 2009

Sequential phases in the development of Aire-expressing medullary thymic epithelial cells involve distinct cellular input.

European journal of immunology

White AJ,Withers DR,Parnell SM,Scott HS,Finke D,Lane PJ,Jenkinson EJ,Anderson G

35-0042 was used in Flow cytometry/Cell sorting to analyse the cellular and molecular requirements for the initial appearance of Aire+ medullary thymic epithelial cells in the embryonic thymus, in addition to their persistence in the adult thymus.

Tue Apr 01 00:00:00 EDT 2008

Bioinformatics

Protein Aliases: CD4; CD4 antigen p55; cd4a; fCD4; Leu-3; T-cell differentiation antigen L3T4; T-cell surface antigen T4/Leu-3; T-cell surface glycoprotein CD4; T-cell surface glycoprotein CD4 precursor (T-cell surface antigen T4/Leu-3) (T-cell differentiation antigen L3T4)

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Gene Aliases: Cd4; L3T4; Ly-4

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UniProt ID: (Mouse) P06332

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Entrez Gene ID: (Mouse) 12504

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Function(s)

glycoprotein binding protein binding zinc ion binding coreceptor activity enzyme binding protein kinase binding protein homodimerization activity

Process(es)

cytokine production immune system process induction by virus of host cell-cell fusion immune response cell adhesion cell surface receptor signaling pathway T cell differentiation maintenance of protein location in cell T cell activation T cell selection protein palmitoleylation positive regulation of protein kinase activity positive regulation of peptidyl-tyrosine phosphorylation defense response to Gram-negative bacterium positive regulation of calcium-mediated signaling regulation of T cell activation positive regulation of T cell activation

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CD4 Monoclonal Antibody (RM4-5), PE-Cyanine5.5, eBioscience™

CD4 Antibody (35-0042-82) in Flow

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35-0042-82

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