Invitrogen

CD40 Monoclonal Antibody (HM40-3), eBioscience™

FIGURE: 1 / 12

CD40 Antibody (14-0402-82) in Flow

Staining of BALB/c splenocytes with 0.5 µg Armenian Hamster IgG Isotype Control Purified (Product # 14-4888-81) (open histogram) or 0.5 µg Anti-Mouse/Rat CD40 Purified (filled histogram) followed by Anti-Armenian Hamster IgG FITC (Product # 11-4111). Total viable cells were used for analysis.

Product Image Gallery

Product Details

14-0402-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

Assay-Dependent

View 3 publications 3 publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 18 publications 18 publications

Functional Assay (Functional)

Assay-Dependent

View 9 publications 9 publications

In vitro Assay (IV)

View 5 publications 5 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse, Rat

Published species

Human, Mouse, Rat

Host/Isotype

Armenian hamster / IgM, kappa

Class

Monoclonal

Type

Antibody

Clone

HM40-3

Conjugate

Unconjugated Unconjugated Unconjugated

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_467228

Product Specific Information

Description: The HM40-3 monoclonal antibody reacts with mouse and rat CD40, a 45-50 kDa type I transmembrane glycoprotein. CD40 is a member of the TNFR family and is expressed by mouse B lymphocytes, follicular dendritic cells, thymic epithelium, and a subset of peripheral T cells. CD40 regulates B cell development/maturation by inducing Ig isotype switching and in combination with other signals such as IL-4, protects B cells from surface Ig-induced apoptosis and promotes proliferation. Interaction of CD40 with CD154 (gp39), its ligand on T cells, is important in T-B cell crosstalk and plays a role in costimulation and immune regulation.

Applications Reported: The HM40-3 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining. HM40-3 has also been reported in inhibition of binding of soluble CD154 to CD40 and in vitro activation of B cells. (Please use Functional Grade purified HM40-3, Product # 16-0402, in functional assays).

Applications Tested: The HM40-3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD40 antigen is a single chain glycoprotein that is known to be a member of the tumor necrosis factor/nerve growth factor superfamily and shows a significant homology to the Hodgkin's disease associated antigen, CD30. CD40 is present on all B cells except plasma cells and is also found on some epithelial cells, carcinomas and lymphoid dendritic cells. CD40 has been found to be essential in mediating a broad variety of immune and inflammatory responses including T cell-dependent immunoglobulin class switching, memory B cell development, and germinal center formation. AT-hook transcription factor AKNA is reported to coordinately regulate the expression of CD40, which may be important for homotypic cell interactions. Adaptor protein TNFR2 interacts with CD40 and serves as a mediator of the signal transduction. The interaction of CD40 and its ligand is found to be necessary for amyloid-beta-induced microglial activation, and thus is thought to be an early event in Alzheimer disease pathogenesis. Two alternatively spliced transcript variants of CD40 encoding distinct isoforms have been reported. Diseases associated with CD40 dysfunction include Type 3 Hyper-Igm immunodeficiency and CD40 ligand deficiency.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Carvacrol protects mice against LPS-induced sepsis and attenuates inflammatory response in macrophages by modulating the ERK1/2 pathway.

Scientific reports

Yan C,Kuang W,Jin L,Wang R,Niu L,Xie C,Ding J,Liao Y,Wang L,Wan H,Ma G

Published figure using CD40 monoclonal antibody (Product # 14-0402-82) in Flow Cytometry

Mon Aug 07 00:00:00 EDT 2023

Curcumin-dependent phenotypic transformation of microglia mediates resistance to pseudorabies-induced encephalitis.

Veterinary research

Feng L,Luo G,Li Y,Zhang C,Liu Y,Liu Y,Chen H,He D,Zhu Y,Gan L

Published figure using CD40 monoclonal antibody (Product # 14-0402-82) in Flow Cytometry

Tue Mar 14 00:00:00 EDT 2023

PP2Cδ Controls the Differentiation and Function of Dendritic Cells Through Regulating the NSD2/mTORC2/ACLY Pathway.

Frontiers in immunology

Lv N,Jin S,Liang Z,Wu X,Kang Y,Su L,Dong Y,Wang B,Ma T,Shi L

Published figure using CD40 monoclonal antibody (Product # 14-0402-82) in Flow Cytometry

Mon Feb 28 00:00:00 EST 2022

Microglia Demonstrate Local Mixed Inflammation and a Defined Morphological Shift in an APP/PS1 Mouse Model.

Journal of Alzheimer's disease : JAD

Holloway OG,King AE,Ziebell JM

14-0402 was used in Immunohistochemistry to indicate that pro- and anti-inflammatory factors of microglia occur in APP/PS1 mice.

Fri Sep 10 00:00:00 EDT 2021

REV7 is required for processing AID initiated DNA lesions in activated B cells.

Nature communications

Yang D,Sun Y,Chen J,Zhang Y,Fan S,Huang M,Xie X,Cai Y,Shang Y,Gui T,Sun L,Hu J,Dong J,Yeap LS,Wang X,Xiao W,Meng FL

Published figure using CD40 monoclonal antibody (Product # 14-0402-82) in In vitro Assay

Thu Jun 04 00:00:00 EDT 2020

REV7 is required for processing AID initiated DNA lesions in activated B cells.

Nature communications

Yang D,Sun Y,Chen J,Zhang Y,Fan S,Huang M,Xie X,Cai Y,Shang Y,Gui T,Sun L,Hu J,Dong J,Yeap LS,Wang X,Xiao W,Meng FL

Published figure using CD40 monoclonal antibody (Product # 14-0402-82) in In vitro Assay

Thu Jun 04 00:00:00 EDT 2020

Myeloid loss of Beclin 1 promotes PD-L1hi precursor B cell lymphoma development.

The Journal of clinical investigation

Tan P,He L,Xing C,Mao J,Yu X,Zhu M,Diao L,Han L,Zhou Y,You MJ,Wang HY,Wang RF

14-0402-82 was used in Flow Cytometry to identify myeloid Becn1 as a key regulator of cancer immunity and therapeutic target for pre-B cell lymphomas.

Mon Dec 02 00:00:00 EST 2019

TET enzymes control antibody production and shape the mutational landscape in germinal centre B cells.

The FEBS journal

Schoeler K,Aufschnaiter A,Messner S,Derudder E,Herzog S,Villunger A,Rajewsky K,Labi V

Published figure using CD40 monoclonal antibody (Product # 14-0402-82) in Flow Cytometry

Sun Sep 01 00:00:00 EDT 2019

cGAS/STING/TBK1/IRF3 Signaling Pathway Activates BMDCs Maturation Following Mycobacterium bovis Infection.

International journal of molecular sciences

Li Q,Liu C,Yue R,El-Ashram S,Wang J,He X,Zhao D,Zhou X,Xu L

Published figure using CD40 monoclonal antibody (Product # 14-0402-82) in Flow Cytometry

Tue Feb 19 00:00:00 EST 2019

CRISPR/Cas9 Screens Reveal Multiple Layers of B cell CD40 Regulation.

Cell reports

Jiang C,Trudeau SJ,Cheong TC,Guo R,Teng M,Wang LW,Wang Z,Pighi C,Gautier-Courteille C,Ma Y,Jiang S,Wang C,Zhao B,Paillard L,Doench JG,Chiarle R,Gewurz BE

14-0402-82 was used in Flow Cytometry to produce CRISPR/Cas9 genome-scale screens that allowed the identification of additional mechanisms by which CD40 is regulated in B cells.

Tue Jul 30 00:00:00 EDT 2019

Effect of immune tolerance induced by immature dendritic cells and CTLA4-Ig on systemic lupus erythematosus: An in vivo study.

Experimental and therapeutic medicine

Huang C,Zhang L,Ling F,Wen S,Luo Y,Liu H,Liu J,Zheng W,Liang M,Sun J,Lin YK

Published figure using CD40 monoclonal antibody (Product # 14-0402-82) in Flow Cytometry

Thu Mar 01 00:00:00 EST 2018

Systemic ST6Gal-1 Is a Pro-survival Factor for Murine Transitional B Cells.

Frontiers in immunology

Irons EE,Lau JTY

14-0402-82 was used in Functional assay to investigate the contributions of B cell intrinsic and extrinsic ST6Gal-1 to B cell development.

Fri Oct 25 00:00:00 EDT 2019

Essential role for the transcription factor Bhlhe41 in regulating the development, self-renewal and BCR repertoire of B-1a cells.

Nature immunology

Kreslavsky T,Vilagos B,Tagoh H,Poliakova DK,Schwickert TA,Wöhner M,Jaritz M,Weiss S,Taneja R,Rossner MJ,Busslinger M

14-0402 was used in Flow cytometry/Cell sorting to elucidate the role of the transcription factor Bhlhe41, with a lesser contribution by Bhlhe40, in controlling B-1a cell differentiation.

Sat Apr 01 00:00:00 EDT 2017

Environmental sensing by mature B cells is controlled by the transcription factors PU.1 and SpiB.

Nature communications

Willis SN,Tellier J,Liao Y,Trezise S,Light A,O'Donnell K,Garrett-Sinha LA,Shi W,Tarlinton DM,Nutt SL

Published figure using CD40 monoclonal antibody (Product # 14-0402-82) in Flow Cytometry

Fri Nov 10 00:00:00 EST 2017

Der f 31, a novel allergen from Dermatophagoides farinae, activates epithelial cells and enhances lung-resident group 2 innate lymphoid cells.

Scientific reports

Wang H,Lin J,Zeng L,Ouyang C,Ran P,Yang P,Liu Z

Published figure using CD40 monoclonal antibody (Product # 14-0402-82) in Flow Cytometry

Thu Aug 17 00:00:00 EDT 2017

Intrapancreatic injection of human bone marrow-derived mesenchymal stem/stromal cells alleviates hyperglycemia and modulates the macrophage state in streptozotocin-induced type 1 diabetic mice.

PloS one

Murai N,Ohtaki H,Watanabe J,Xu Z,Sasaki S,Yagura K,Shioda S,Nagasaka S,Honda K,Izumizaki M

14-0402 was used in Immunohistochemistry to evaluate the effects of intrapancreatic and intravenous injection of human BMMSCs in streptozotocin-induced type-1 diabetic mouse model.

Mon Nov 20 00:00:00 EST 2017

Blood Stem Cell Activity Is Arrested by Th1-Mediated Injury Preventing Engraftment following Nonmyeloablative Conditioning.

Journal of immunology (Baltimore, Md. : 1950)

Müller AM,Florek M,Kohrt HE,Küpper NJ,Filatenkov A,Linderman JA,Hadeiba H,Negrin RS,Shizuru JA

14-0402 was used in Flow cytometry/Cell sorting to shed new light on the effects of CD4+ T cells on HSC biology.

Tue Nov 15 00:00:00 EST 2016

Thiol peroxiredoxin, a novel allergen from Bombyx mori, modulates functions of macrophages and dendritic cells.

American journal of translational research

Wang H,Hu W,Liang Z,Zeng L,Li J,Yan H,Yang P,Liu Z,Wang L

Published figure using CD40 monoclonal antibody (Product # 14-0402-82) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

Transcription factor c-Rel plays a crucial role in driving anti-CD40-mediated innate colitis.

Mucosal immunology

Visekruna A,Linnerz T,Martinic V,Vachharajani N,Hartmann S,Harb H,Joeris T,Pfefferle PI,Hofer MJ,Steinhoff U

14-0402 was used in Flow cytometry/Cell sorting to investigate the role c-Rel plays in regulating innate lymphoid cells which induce anti-CD40-mediated innate colitis in mice.

Sun Mar 01 00:00:00 EST 2015

HP-1γ Controls High-Affinity Antibody Response to T-Dependent Antigens.

Frontiers in immunology

Ha N,Pham DH,Shahsafaei A,Naruse C,Asano M,Thai TH

14-0402 was used in in vitro experiments to provide in vivo evidence for the non-redundant role of HP-1γ in immunity.

Fri Jun 27 00:00:00 EDT 2014

Binding of AID to DNA does not correlate with mutator activity.

Journal of immunology (Baltimore, Md. : 1950)

Matthews AJ,Husain S,Chaudhuri J

14-0402 was used in Functional assays to show a lack of correlation between AID binding and its mutator activity, implying the presence of downstream effectors.

Tue Jul 01 00:00:00 EDT 2014

DNA vaccine encoding CD40 targeted to dendritic cells in situ prevents the development of Heymann nephritis in rats.

Kidney international

Wang Y,Wang YM,Wang Y,Zheng G,Zhang GY,Zhou JJ,Tan TK,Cao Q,Hu M,Watson D,Wu H,Zheng D,Wang C,Lahoud MH,Caminschi I,Harris DC,Alexander SI

14-0402 was used in in vitro experiments to assess the effects of a CD40 DNA vaccine on disease development in an experimental rat model of human autoimmune-mediated membranous nephritis.

Fri Feb 01 00:00:00 EST 2013

Generation of hypoallergenic neoglycoconjugates for dendritic cell targeted vaccination: a novel tool for specific immunotherapy.

Journal of controlled release : official journal of the Controlled Release Society

Weinberger EE,Himly M,Myschik J,Hauser M,Altmann F,Isakovic A,Scheiblhofer S,Thalhamer J,Weiss R

14-0402 was used in Flow cytometry/Cell sorting to investigate carbohydrate-mediated targeting of allergens to dermal antigen-presenting cells and its influence on immunogenicity and allergenicity.

Mon Jan 28 00:00:00 EST 2013

DNA damage activates a complex transcriptional response in murine lymphocytes that includes both physiological and cancer-predisposition programs.

BMC genomics

Innes CL,Hesse JE,Palii SS,Helmink BA,Holub AJ,Sleckman BP,Paules RS

Published figure using CD40 monoclonal antibody (Product # 14-0402-82) in Flow Cytometry

Tue Mar 12 00:00:00 EDT 2013

Synergy between PI3K signaling and MYC in Burkitt lymphomagenesis.

Cancer cell

Sander S,Calado DP,Srinivasan L,Köchert K,Zhang B,Rosolowski M,Rodig SJ,Holzmann K,Stilgenbauer S,Siebert R,Bullinger L,Rajewsky K

14-0402 was used in Immunohistochemistry on frozen tissues to find recurrent PI3K pathway activation in human Burkitt lymphoma indicates that deregulated c-MYC and PI3K activity cooperate in Burkitt lymphoma pathogenesis.

Tue Aug 14 00:00:00 EDT 2012

Essential role of EBF1 in the generation and function of distinct mature B cell types.

The Journal of experimental medicine

Vilagos B,Hoffmann M,Souabni A,Sun Q,Werner B,Medvedovic J,Bilic I,Minnich M,Axelsson E,Jaritz M,Busslinger M

14-0402 was used in Magnetic cell separation to study the role of the transcription factor EBF1 during B cell development using gain- and loss-of-function analyses.

Mon Apr 09 00:00:00 EDT 2012

Immunogenicity and protective capacity of a virosomal respiratory syncytial virus vaccine adjuvanted with monophosphoryl lipid A in mice.

PloS one

Kamphuis T,Meijerhof T,Stegmann T,Lederhofer J,Wilschut J,de Haan A

Published figure using CD40 monoclonal antibody (Product # 14-0402-82) in Flow Cytometry

Wed Sep 19 00:00:00 EDT 2012

Roles of the kinase TAK1 in TRAF6-dependent signaling by CD40 and its oncogenic viral mimic, LMP1.

PloS one

Arcipowski KM,Bishop GA

14-0402 was used in Functional assays to determine that TAK1 is required for JNK activation and interleukin-6 production mediated by CD40 and LMP1.

Mon Jan 14 00:00:00 EST 2013

Roles of the kinase TAK1 in TRAF6-dependent signaling by CD40 and its oncogenic viral mimic, LMP1.

PloS one

Arcipowski KM,Bishop GA

14-0402 was used in Functional assays to determine that TAK1 is required for JNK activation and interleukin-6 production mediated by CD40 and LMP1.

Mon Jan 14 00:00:00 EST 2013

Humanized c-Myc mouse.

PloS one

Lehmann FM,Feicht S,Helm F,Maurberger A,Ladinig C,Zimber-Strobl U,Kühn R,Mautner J,Gerbitz A,Bornkamm GW

14-0402-82 was used in Functional assay to develop mouse strains expressing the human proto-oncogene product c-MYC in tumour and normal cells.

Mon Jan 14 00:00:00 EST 2013

The splicing regulator PTBP2 interacts with the cytidine deaminase AID and promotes binding of AID to switch-region DNA.

Nature immunology

Nowak U,Matthews AJ,Zheng S,Chaudhuri J

14-0402 was used in in vitro experiments to elucidate the binding mechanism of the cytidine deaminase, AID, to DNA switch regions during class-switch recombination.

Tue Feb 01 00:00:00 EST 2011

CD19-independent instruction of murine marginal zone B-cell development by constitutive Notch2 signaling.

Blood

Hampel F,Ehrenberg S,Hojer C,Draeseke A,Marschall-Schröter G,Kühn R,Mack B,Gires O,Vahl CJ,Schmidt-Supprian M,Strobl LJ,Zimber-Strobl U

14-0402 was used in in vitro experiments to implicate that a constitutive Notch2 signal in transitional type 1 B cells is sufficient to drive MZ B-cell differentiation.

Thu Dec 08 00:00:00 EST 2011

B cells lacking the tumor suppressor TNFAIP3/A20 display impaired differentiation and hyperactivation and cause inflammation and autoimmunity in aged mice.

Blood

Chu Y,Vahl JC,Kumar D,Heger K,Bertossi A,Wójtowicz E,Soberon V,Schenten D,Mack B,Reutelshöfer M,Beyaert R,Amann K,van Loo G,Schmidt-Supprian M

14-0402-82 was used in Functional assay to examine the requirement of A20 for the normal differentiation of the marginal zone B and B1 cell subsets.

Thu Feb 17 00:00:00 EST 2011

Constitutive canonical NF-κB activation cooperates with disruption of BLIMP1 in the pathogenesis of activated B cell-like diffuse large cell lymphoma.

Cancer cell

Calado DP,Zhang B,Srinivasan L,Sasaki Y,Seagal J,Unitt C,Rodig S,Kutok J,Tarakhovsky A,Schmidt-Supprian M,Rajewsky K

14-0402 was used in Functional assays to study the constitutive activation of canonical NF-κB signalling with BLIMP1 disruption in the development of a lymphoma resembling activated B-cell(ABC)-diffuse large B-cell lymphoma(DLBL).

Tue Dec 14 00:00:00 EST 2010

Latent membrane protein 1, the EBV-encoded oncogenic mimic of CD40, accelerates autoimmunity in B6.Sle1 mice.

Journal of immunology (Baltimore, Md. : 1950)

Peters AL,Stunz LL,Meyerholz DK,Mohan C,Bishop GA

14-0402-82 was used in Functional assay to investigate whether LMP1-mediated activation signals cooperate with and/or amplify events that predispose individuals to the development of autoimmunity.

Fri Oct 01 00:00:00 EDT 2010

Critical role of microglial CD40 in the maintenance of mechanical hypersensitivity in a murine model of neuropathic pain.

European journal of immunology

Cao L,Palmer CD,Malon JT,De Leo JA

14-0402 was used in Immunohistochemistry to investigate the role of CD40 in the development of mechanical hypersensitivity after spinal nerve L5 transection in mice.

Tue Dec 01 00:00:00 EST 2009

Genomic instability resulting from Blm deficiency compromises development, maintenance, and function of the B cell lineage.

Journal of immunology (Baltimore, Md. : 1950)

Babbe H,McMenamin J,Hobeika E,Wang J,Rodig SJ,Reth M,Leder P

14-0402-82 was used in Functional assay to investigate the role of the RecQ family helicase, BLM, in B cell biology.

Thu Jan 01 00:00:00 EST 2009

CD40 ligand promotes Mac-1 expression, leukocyte recruitment, and neointima formation after vascular injury.

The American journal of pathology

Li G,Sanders JM,Bevard MH,Sun Z,Chumley JW,Galkina EV,Ley K,Sarembock IJ

14-0402 was used in Functional assays to study the function of CD40 ligand in neointima formation following vascular injury in an atherogenic animal model.

Tue Apr 01 00:00:00 EDT 2008

Initiation of protein O glycosylation by the polypeptide GalNAcT-1 in vascular biology and humoral immunity.

Molecular and cellular biology

Tenno M,Ohtsubo K,Hagen FK,Ditto D,Zarbock A,Schaerli P,von Andrian UH,Ley K,Le D,Tabak LA,Marth JD

14-0402 was used in Flow cytometry/Cell sorting to find that ppGalNAcT-1 plays key roles in cell and glycoprotein selective functions that modulate the haematopoietic system.

Sat Dec 01 00:00:00 EST 2007

Bioinformatics

Protein Aliases: B-cell surface antigen CD40; Bp50; CD antigen CD40; CD40; CD40 antigen, TNF receptor superfamily member 5; CD40L receptor; Immunoglobulin M; ImmunoglobulinM; MGC9013; sCD40; soluble CD40; T-cell differentiation antigen; Tumor necrosis factor receptor superfamily member 5; tumor necrosis factor receptor superfamily, member 5

View more View less

Gene Aliases: AI326936; Bp50; Cd40; GP39; HIGM1; IGM; IMD3; p50; T-BAM; Tnfrsf5; TRAP

View more View less

UniProt ID: (Mouse) Q99NE2

View more View less

Entrez Gene ID: (Rat) 171369, (Mouse) 21939

View more View less

Function(s)

antigen binding protein binding enzyme binding ubiquitin protein ligase binding transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD40 Monoclonal Antibody (HM40-3), eBioscience™

CD40 Antibody (14-0402-82) in Flow

Product Details

14-0402-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Functional Assay (Functional)

In vitro Assay (IV)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!