Invitrogen

CD45.1 Monoclonal Antibody (A20), Biotin, eBioscience™

25 Published Figures

49 References

View all (40) CD45.1 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (25)

CD45.1 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 26

CD45.1 Antibody (13-0453-82) in Flow

Staining of SJL mouse splenocytes with 0.125 µg of Mouse IgG2a kappa Isotype Control Biotin (Product # 13-4724-85) (open histogram) or 0.125 µg of Anti-Mouse CD45.1 Biotin (filled histogram) followed by Streptavidin PE (Product # 12-4317-87). Cells in the lymphocyte gate were used for analysis.

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Product Details

13-0453-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 4 publications 4 publications

Flow Cytometry (Flow)

0.25 µg/test

View 44 publications 44 publications

Product Specifications

Species Reactivity

Mouse

Published species

Fish, Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

A20

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466453

Product Specific Information

Description: The A20 monoclonal antibody reacts with the mouse CD45 molecule, the leukocyte common antigen (LCA) in CD45.1-expressing mouse strains. The strains that express CD45.1 include SJL/J, DA, STS/A and RIII. CD45.1 is expressed by all leukocytes in these strains.

Applications Reported: The A20 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining of frozen tissue sections.

Applications Tested: The A20 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45 (LCA, leukocyte common antigen) is a receptor-type protein tyrosine phosphatase ubiquitously expressed in all nucleated hematopoietic cells, comprising approximately 10% of all surface proteins in lymphocytes. CD45 glycoprotein is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases. CD45 protein exists as multiple isoforms as a result of alternative splicing; these isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. These CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45RA is an isoform of the CD45 complex and has restricted expression between different subtypes of lymphoid cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Tet-mediated DNA demethylation regulates specification of hematopoietic stem and progenitor cells during mammalian embryogenesis.

Science advances

Ma L,Tang Q,Gao X,Lee J,Lei R,Suzuki M,Zheng D,Ito K,Frenette PS,Dawlaty MM

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Fri Mar 04 00:00:00 EST 2022

Smc3 dosage regulates B cell transit through germinal centers and restricts their malignant transformation.

Nature immunology

Rivas MA,Meydan C,Chin CR,Challman MF,Kim D,Bhinder B,Kloetgen A,Viny AD,Teater MR,McNally DR,Doane AS,Béguelin W,Fernández MTC,Shen H,Wang X,Levine RL,Chen Z,Tsirigos A,Elemento O,Mason CE,Melnick AM

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2021

Aged hematopoietic stem cells are refractory to bloodborne systemic rejuvenation interventions.

The Journal of experimental medicine

Ho TT,Dellorusso PV,Verovskaya EV,Bakker ST,Flach J,Smith LK,Ventura PB,Lansinger OM,Hérault A,Zhang SY,Kang YA,Mitchell CA,Villeda SA,Passegué E

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Mon Jul 05 00:00:00 EDT 2021

Differentiation of fetal hematopoietic stem cells requires ARID4B to restrict autocrine KITLG/KIT-Src signaling.

Cell reports

Young IC,Wu B,Andricovich J,Chuang ST,Li R,Tzatsos A,Wu RC,Wu MY

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Tue Nov 23 00:00:00 EST 2021

Development of a VRC01-class germline targeting immunogen derived from anti-idiotypic antibodies.

Cell reports

Seydoux E,Wan YH,Feng J,Wall A,Aljedani S,Homad LJ,MacCamy AJ,Weidle C,Gray MD,Brumage L,Taylor JJ,Pancera M,Stamatatos L,McGuire AT

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Tue May 04 00:00:00 EDT 2021

Ddb1 Is Essential for the Expansion of CD4⁺ Helper T Cells by Regulating Cell Cycle Progression and Cell Death.

Frontiers in immunology

Yang L,Chen W,Li L,Xiao Y,Fan S,Zhang Q,Xia T,Li M,Hong Y,Zhao T,Li Q,Liu WH,Xiao N

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Mon Dec 20 00:00:00 EST 2021

Deregulated Notch and Wnt signaling activates early-stage myeloid regeneration pathways in leukemia.

The Journal of experimental medicine

Kang YA,Pietras EM,Passegué E

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Mon Mar 02 00:00:00 EST 2020

RIPK3 and Caspase-1/11 Are Necessary for Optimal Antigen-Specific CD8 T Cell Response Elicited by Genetically Modified Listeria monocytogenes.

Frontiers in immunology

Rana A,de Almeida FC,Paico Montero HA,Gonzales Carazas MM,Bortoluci KR,Sad S,Amarante-Mendes GP

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Fri Mar 26 00:00:00 EDT 2021

Engineering monocyte/macrophage-specific glucocerebrosidase expression in human hematopoietic stem cells using genome editing.

Nature communications

Scharenberg SG,Poletto E,Lucot KL,Colella P,Sheikali A,Montine TJ,Porteus MH,Gomez-Ospina N

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Fri Jul 03 00:00:00 EDT 2020

Attenuation of TCR-induced transcription by Bach2 controls regulatory T cell differentiation and homeostasis.

Nature communications

Sidwell T,Liao Y,Garnham AL,Vasanthakumar A,Gloury R,Blume J,Teh PP,Chisanga D,Thelemann C,de Labastida Rivera F,Engwerda CR,Corcoran L,Kometani K,Kurosaki T,Smyth GK,Shi W,Kallies A

13-0453-82 was used in Flow Cytometry to show homeostasis of thymically-derived and peripherally-derived Treg cells is maintained through the balancing of TCR signaling induced transcriptional activity by Bach2.

Tue Jan 14 00:00:00 EST 2020

T_H17 cells require ongoing classic IL-6 receptor signaling to retain transcriptional and functional identity.

Science immunology

Harbour SN,DiToro DF,Witte SJ,Zindl CL,Gao M,Schoeb TR,Jones GW,Jones SA,Hatton RD,Weaver CT

13-0453-82 was used in Immunohistochemistry (Frozen) to find that IL-6 signaling is required for both induction and maintenance of mouse TH17 cells; IL-6Rα-deficient TH17 cells rapidly lost their TH17 phenotype and did not cause disease in two models of colitis.

Fri Jul 17 00:00:00 EDT 2020

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

Interleukin-21 Induces Short-Lived Effector CD8⁺ T Cells but Does Not Inhibit Their Exhaustion after Mycobacterium bovis BCG Infection in Mice.

Infection and immunity

Noguchi N,Nakamura R,Hatano S,Yamada H,Sun X,Ohara N,Yoshikai Y

Wed Aug 01 00:00:00 EDT 2018

The H3K4 methyltransferase Setd1b is essential for hematopoietic stem and progenitor cell homeostasis in mice.

eLife

Schmidt K,Zhang Q,Tasdogan A,Petzold A,Dahl A,Arneth BM,Slany R,Fehling HJ,Kranz A,Stewart AF,Anastassiadis K

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Tue Jun 19 00:00:00 EDT 2018

An immune cell spray (ICS) formulation allows for the delivery of functional monocyte/macrophages.

Scientific reports

Beneke V,Küster F,Neehus AL,Hesse C,Lopez-Rodriguez E,Haake K,Rafiei Hashtchin A,Schott JW,Walter D,Braun A,Wolkers WF,Ackermann M,Lachmann N

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Fri Nov 02 00:00:00 EDT 2018

Egr2 and 3 control adaptive immune responses by temporally uncoupling expansion from T cell differentiation.

The Journal of experimental medicine

Miao T,Symonds ALJ,Singh R,Symonds JD,Ogbe A,Omodho B,Zhu B,Li S,Wang P

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Mon Jun 05 00:00:00 EDT 2017

The Deubiquitinase OTULIN Is an Essential Negative Regulator of Inflammation and Autoimmunity.

Cell

Damgaard RB,Walker JA,Marco-Casanova P,Morgan NV,Titheradge HL,Elliott PR,McHale D,Maher ER,McKenzie ANJ,Komander D

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Thu Aug 25 00:00:00 EDT 2016

Murine iPSC-Derived Macrophages as a Tool for Disease Modeling of Hereditary Pulmonary Alveolar Proteinosis due to Csf2rb Deficiency.

Stem cell reports

Mucci A,Kunkiel J,Suzuki T,Brennig S,Glage S,Kühnel MP,Ackermann M,Happle C,Kuhn A,Schambach A,Trapnell BC,Hansen G,Moritz T,Lachmann N

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Tue Aug 09 00:00:00 EDT 2016

Neutrophil ageing is regulated by the microbiome.

Nature

Zhang D,Chen G,Manwani D,Mortha A,Xu C,Faith JJ,Burk RD,Kunisaki Y,Jang JE,Scheiermann C,Merad M,Frenette PS

13-0453 was used in Flow cytometry/Cell sorting to show that neutrophil pro-inflammatory activity correlates positively with their ageing whilst in circulation.

Thu Sep 24 00:00:00 EDT 2015

MHC-mismatched mixed chimerism augments thymic regulatory T-cell production and prevents relapse of EAE in mice.

Proceedings of the National Academy of Sciences of the United States of America

Wu L,Li N,Zhang M,Xue SL,Cassady K,Lin Q,Riggs AD,Zeng D

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Tue Dec 29 00:00:00 EST 2015

Alternatively activated macrophages promote pancreatic fibrosis in chronic pancreatitis.

Nature communications

Xue J,Sharma V,Hsieh MH,Chawla A,Murali R,Pandol SJ,Habtezion A

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Mon May 18 00:00:00 EDT 2015

Heme-mediated SPI-C induction promotes monocyte differentiation into iron-recycling macrophages.

Cell

Haldar M,Kohyama M,So AY,Kc W,Wu X,Briseño CG,Satpathy AT,Kretzer NM,Arase H,Rajasekaran NS,Wang L,Egawa T,Igarashi K,Baltimore D,Murphy TL,Murphy KM

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Thu Mar 13 00:00:00 EDT 2014

WASH is required for the differentiation commitment of hematopoietic stem cells in a c-Myc-dependent manner.

The Journal of experimental medicine

Xia P,Wang S,Huang G,Zhu P,Li M,Ye B,Du Y,Fan Z

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Mon Sep 22 00:00:00 EDT 2014

Lymph node stromal cells constrain immunity via MHC class II self-antigen presentation.

eLife

Baptista AP,Roozendaal R,Reijmers RM,Koning JJ,Unger WW,Greuter M,Keuning ED,Molenaar R,Goverse G,Sneeboer MM,den Haan JM,Boes M,Mebius RE

13-0453 was used in Flow cytometry/Cell sorting to investigate the role of MHC-II in lymph node stromal cells, showing that stromal cells constrain immunity through MHC-II expression.

Wed Nov 19 00:00:00 EST 2014

NK cell-extrinsic IL-18 signaling is required for efficient NK-cell activation by vaccinia virus.

European journal of immunology

Brandstadter JD,Huang X,Yang Y

13-0453 was used in Flow cytometry/Cell sorting to provide insights into the design of effective NK-cell-based therapies for viral infections and cancer.

Mon Sep 01 00:00:00 EDT 2014

Mutual expression of the transcription factors Runx3 and ThPOK regulates intestinal CD4⁺ T cell immunity.

Nature immunology

Reis BS,Rogoz A,Costa-Pinto FA,Taniuchi I,Mucida D

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2013

In vivo generation of transplantable human hematopoietic cells from induced pluripotent stem cells.

Blood

Amabile G,Welner RS,Nombela-Arrieta C,D'Alise AM,Di Ruscio A,Ebralidze AK,Kraytsberg Y,Ye M,Kocher O,Neuberg DS,Khrapko K,Silberstein LE,Tenen DG

13-0453 was used in Flow cytometry/Cell sorting to develop an in vivo system in which human induced pluripotent stem cells differentiate within teratomas to derive functional myeloid and lymphoid cells.

Thu Feb 21 00:00:00 EST 2013

Arrayed lentiviral barcoding for quantification analysis of hematopoietic dynamics.

Stem cells (Dayton, Ohio)

Grosselin J,Sii-Felice K,Payen E,Chretien S,Tronik-Le Roux D,Leboulch P

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Tue Oct 01 00:00:00 EDT 2013

Bcl11a controls Flt3 expression in early hematopoietic progenitors and is required for pDC development in vivo.

PloS one

Wu X,Satpathy AT,Kc W,Liu P,Murphy TL,Murphy KM

13-0453 was used in Flow cytometry/Cell sorting to investigate the role of Bcl11a in the development of dendritic cells, showing that it controls Flt3 expression in early hematopoietic progenitors and is required for pDC development.

Fri Jan 10 00:00:00 EST 2014

Plasmacytoid dendritic cells transport peripheral antigens to the thymus to promote central tolerance.

Immunity

Hadeiba H,Lahl K,Edalati A,Oderup C,Habtezion A,Pachynski R,Nguyen L,Ghodsi A,Adler S,Butcher EC

13-0453 was used in Immunohistochemistry on frozen tissues to explore the mechanisms underlying the contribution to immune tolerance conducted by peripheral dendritic cells.

Fri Mar 23 00:00:00 EDT 2012

Thymus-autonomous T cell development in the absence of progenitor import.

The Journal of experimental medicine

Martins VC,Ruggiero E,Schlenner SM,Madan V,Schmidt M,Fink PJ,von Kalle C,Rodewald HR

13-0453 was used in Flow cytometry/Cell sorting to characterise the previously unrecognised capacity of the thymus to sustain T cell development during bone marrow progenitor deprivation.

Mon Jul 30 00:00:00 EDT 2012

T cell apoptosis and induction of Foxp3+ regulatory T cells underlie the therapeutic efficacy of CD4 blockade in experimental autoimmune encephalomyelitis.

Journal of immunology (Baltimore, Md. : 1950)

Duarte J,Carrié N,Oliveira VG,Almeida C,Agua-Doce A,Rodrigues L,Simas JP,Mars LT,Graca L

13-0453 was used in Flow cytometry/Cell sorting to investigate the mechanism underlying effective MS prevention, showing that apoptosis of T cells and induction of Foxp3+ Tregs underlie efficacy of CD4 blockade in EAE.

Wed Aug 15 00:00:00 EDT 2012

Direct reduction of antigen receptor expression in polyclonal B cell populations developing in vivo results in light chain receptor editing.

Journal of immunology (Baltimore, Md. : 1950)

Shen S,Manser T

13-0453 was used in Flow cytometry/Cell sorting to test the role of BCR expression plays in receptor editing in an polyclonal, in vivo population of B cells.

Sun Jan 01 00:00:00 EST 2012

In vitro Ag Cross-presentation and in vivo Ag Cross-presentation by Dendritic Cells in the Mouse.

Bio-protocol

Ghosh M,Shapiro LH

Published figure using CD45.1 monoclonal antibody (Product # 13-0453-82) in Flow Cytometry

Thu Dec 20 00:00:00 EST 2012

IL-6 induced by Staphylococcus aureus infection prevents the induction of skin allograft acceptance in mice.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Ahmed EB,Wang T,Daniels M,Alegre ML,Chong AS

13-0453 was used in Flow cytometry/Cell sorting to reveal that infection with S. aureus, but not P. aeruginosa, at the time of transplantation prohibits successful skin allograft acceptance by inducing inflammation and IL-6 production.

Sun May 01 00:00:00 EDT 2011

Epigenetic changes at Il12rb2 and Tbx21 in relation to plasticity behavior of Th17 cells.

Journal of immunology (Baltimore, Md. : 1950)

Bending D,Newland S,Krejcí A,Phillips JM,Bray S,Cooke A

13-0453 was used in Flow cytometry/Cell sorting to examine the epigenetic mechanisms underpinning murine Th17 plasticity in vivo.

Tue Mar 15 00:00:00 EDT 2011

Nonhematopoietic antigen blocks memory programming of alloreactive CD8+ T cells and drives their eventual exhaustion in mouse models of bone marrow transplantation.

The Journal of clinical investigation

Flutter B,Edwards N,Fallah-Arani F,Henderson S,Chai JG,Sivakumaran S,Ghorashian S,Bennett CL,Freeman GJ,Sykes M,Chakraverty R

13-0453 was used in Flow cytometry/Cell sorting to explore the role of recipient antigen expression by nonhaematopoietic cells in the failure to sustain effective cytotoxic T lymphocyte immunity following blood transplantation.

Mon Nov 01 00:00:00 EDT 2010

Germinal center T follicular helper cell IL-4 production is dependent on signaling lymphocytic activation molecule receptor (CD150).

Journal of immunology (Baltimore, Md. : 1950)

Yusuf I,Kageyama R,Monticelli L,Johnston RJ,Ditoro D,Hansen K,Barnett B,Crotty S

13-0453 was used in Immunofluorescence to study the roles of SAP adaptors and SLAM family receptors in the development and function of T follicular helper cells.

Thu Jul 01 00:00:00 EDT 2010

Intermediate-term hematopoietic stem cells with extended but time-limited reconstitution potential.

Cell stem cell

Benveniste P,Frelin C,Janmohamed S,Barbara M,Herrington R,Hyam D,Iscove NN

13-0453 was used in Flow cytometry/Cell sorting to investigate the presence of "intermediate-term" multipotent hematopoietic stem cells, revealing that these cells are able to sustain clones of differentiating cells for only 4-6 weeks.

Fri Jan 08 00:00:00 EST 2010

Tracking epitope-specific T cells.

Nature protocols

Moon JJ,Chu HH,Hataye J,Pagán AJ,Pepper M,McLachlan JB,Zell T,Jenkins MK

13-0453 was used in Flow cytometry/Cell sorting to track epitope-specific T cells.

Tue Jun 30 00:00:00 EDT 2009

Engagement of TLR2 does not reverse the suppressor function of mouse regulatory T cells, but promotes their survival.

Journal of immunology (Baltimore, Md. : 1950)

Chen Q,Davidson TS,Huter EN,Shevach EM

13-0453 was used in Flow cytometry/Cell sorting to investigate the role of TLRs in adaptive immunity and in regulatory T cells.

Thu Oct 01 00:00:00 EDT 2009

Combined anti-CD40 conditioning and well-timed immunization prolongs CD8+ T cell accumulation and control of established brain tumors.

Journal of immunotherapy (Hagerstown, Md. : 1997)

Ryan CM,Staveley-O'Carroll K,Schell TD

13-0453 was used in Flow cytometry/Cell sorting to investigate the augmentation of CD8+ T-cell-mediated adoptive immunotherapy by targeting a weakly immunogenic epitope.

Mon Jul 13 00:00:00 EDT 2009

miR-150, a microRNA expressed in mature B and T cells, blocks early B cell development when expressed prematurely.

Proceedings of the National Academy of Sciences of the United States of America

Zhou B,Wang S,Mayr C,Bartel DP,Lodish HF

13-0453 was used in Immunofluorescence to study how miR-150 can down-regulate mRNAs important for B cell function, and how it can block further B cell development when expressed prematurely.

Tue Apr 24 00:00:00 EDT 2007

miR-150, a microRNA expressed in mature B and T cells, blocks early B cell development when expressed prematurely.

Proceedings of the National Academy of Sciences of the United States of America

Zhou B,Wang S,Mayr C,Bartel DP,Lodish HF

13-0453 was used in Immunofluorescence to study how miR-150 can down-regulate mRNAs important for B cell function, and how it can block further B cell development when expressed prematurely.

Tue Apr 24 00:00:00 EDT 2007

Hematopoiesis and stem cell renewal in long-term bone marrow cultures containing catalase.

Blood

Gupta R,Karpatkin S,Basch RS

13-0453 was used in Flow cytometry/Cell sorting to investigate the control of haematopoiesis by peroxide-sensitive regulatory mechanisms.

Wed Mar 01 00:00:00 EST 2006

Hematopoiesis and stem cell renewal in long-term bone marrow cultures containing catalase.

Blood

Gupta R,Karpatkin S,Basch RS

13-0453 was used in Flow cytometry/Cell sorting to investigate the control of haematopoiesis by peroxide-sensitive regulatory mechanisms.

Wed Mar 01 00:00:00 EST 2006

Enhanced engraftment of hematopoietic stem/progenitor cells by the transient inhibition of an adaptor protein, Lnk.

Blood

Takizawa H,Kubo-Akashi C,Nobuhisa I,Kwon SM,Iseki M,Taga T,Takatsu K,Takaki S

13-0453 was used in Flow cytometry/Cell sorting to investigate the potential of Lnk for augmented engraftment of HSCs.

Sat Apr 01 00:00:00 EST 2006

Endomucin, a CD34-like sialomucin, marks hematopoietic stem cells throughout development.

The Journal of experimental medicine

Matsubara A,Iwama A,Yamazaki S,Furuta C,Hirasawa R,Morita Y,Osawa M,Motohashi T,Eto K,Ema H,Kitamura T,Vestweber D,Nakauchi H

13-0453 was used in Flow cytometry/Cell sorting to identify for the first time Endomucin as a cell-surface molecule specific to hematopoietic stem cells (HSCs).

Mon Dec 05 00:00:00 EST 2005

Essential roles of CD8+CD122+ regulatory T cells in the maintenance of T cell homeostasis.

The Journal of experimental medicine

Rifa'i M,Kawamoto Y,Nakashima I,Suzuki H

13-0453 was used in Flow cytometry/Cell sorting to investigate the effect of transfer of CD8+CD122+ cells into CD122-deficient neonates, showing that CD8+CD122+ regulatory T cells are essential for T cell homeostasis.

Mon Nov 01 00:00:00 EST 2004

Bioinformatics

Protein Aliases: CD45; CD45 antigen; GP180; L-CA; Leukocyte common antigen; Ly-5; LY5; Lymphocyte antigen 5; lymphocyte common antigen; Receptor-type tyrosine-protein phosphatase C; RT7; T200; T200 glycoprotein

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Gene Aliases: B220; Cd45; CD45R; L-CA; loc; Ly-5; Lyt-4; Ptprc; T200

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UniProt ID: (Mouse) P06800

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Entrez Gene ID: (Mouse) 19264

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Function(s)

phosphoprotein phosphatase activity protein tyrosine phosphatase activity protein binding heparin binding hydrolase activity phosphatase activity protein kinase binding heparan sulfate proteoglycan binding protein phosphatase protein modifying enzyme

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD45.1 Monoclonal Antibody (A20), Biotin, eBioscience™

CD45.1 Antibody (13-0453-82) in Flow

Product Details

13-0453-82

Immunohistochemistry (Frozen) (IHC (F))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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