Invitrogen

CD45RO Monoclonal Antibody (UCHL1), APC, eBioscience™

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This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

3 Published Figures

12 References

View all (42) CD45RO antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

CD45RO Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

CD45RO Antibody (17-0457-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD45RA PE (Product # 12-0458-42) and Mouse IgG2a kappa Isotype Control APC (Product # 17-4724-81) (left) or Anti-Human CD45RO APC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

17-0457-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.03 µg)/test

View 12 publications 12 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rhesus monkey

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

UCHL1

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1907398

Product Specific Information

Description: The UCHL1 monoclonal antibody reacts with human CD45RO, a 180 kDa isoform of CD45. CD45RO is expressed by most thymocytes, activated memory T cells, granulocytes and monocytes. CD22 is a ligand for CD45RO. Expression of CD45RO and CD45RA is used commonly to discriminate subsets of peripheral T cells.

Applications Reported: This UCHL1 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This UCHL1 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

CD45RO (UCHL1, ubiquitin carboxyl terminal esterase L1) is the shortest isoform of a receptor-type protein tyrosine phosphatase, CD45 glycoprotein. CD45 is crucial in lymphocyte development and antigen signaling, serving as an important regulator of Src-family kinases, promotes cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis. CD45 isoforms differ in their extracellular domains, whereas they share identical transmembrane and cytoplasmic domains. CD45 isoforms differ in their ability to translocate into the glycosphingolipid-enriched membrane domains and their expression depends on cell type and physiological state of the cell. CD45RO is expressed on macrophages, CD8+ T cells, activated T cells and myeloma cells. Besides the role in immunoreceptor signaling, CD45 is important in promoting cell survival by modulating integrin-mediated signal transduction pathway and is also involved in DNA fragmentation during apoptosis.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

WT1 Pulsed Human CD141+ Dendritic Cell Vaccine Has High Potential in Solid Tumor-Targeted Immunotherapy.

International journal of molecular sciences

Cho SY,Jeong SM,Jeon YJ,Yang SJ,Hwang JE,Yoo BM,Kim HS

17-0457-42 was used in Flow cytometry/Cell sorting to develop a DC vaccine named CellgramDC-WT1 (CDW).

Thu Jan 12 00:00:00 EST 2023

Serum Amyloid A Proteins Induce Pathogenic Th17 Cells and Promote Inflammatory Disease.

Cell

Lee JY,Hall JA,Kroehling L,Wu L,Najar T,Nguyen HH,Lin WY,Yeung ST,Silva HM,Li D,Hine A,Loke P,Hudesman D,Martin JC,Kenigsberg E,Merad M,Khanna KM,Littman DR

17-0457 was used in Flow cytometry/Cell sorting to suggest that serum amyloid A proteins direct a pathogenic pro-inflammatory Th17 cell differentiation program and promote Th17-mediated inflammatory diseases.

Thu Jan 09 00:00:00 EST 2020

Longitudinal analysis of subtype C envelope tropism for memory CD4⁺ T cell subsets over the first 3 years of untreated HIV-1 infection.

Retrovirology

Gartner MJ,Gorry PR,Tumpach C,Zhou J,Dantanarayana A,Chang JJ,Angelovich TA,Ellenberg P,Laumaea AE,Nonyane M,Moore PL,Lewin SR,Churchill MJ,Flynn JK,Roche M

Published figure using CD45RO monoclonal antibody (Product # 17-0457-42) in Flow Cytometry

Thu Aug 06 00:00:00 EDT 2020

Development of CAR-T cell therapy for B-ALL using a point-of-care approach.

Oncoimmunology

de Macedo Abdo L,Barros LRC,Saldanha Viegas M,Vieira Codeço Marques L,de Sousa Ferreira P,Chicaybam L,Bonamino MH

Published figure using CD45RO monoclonal antibody (Product # 17-0457-42) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD45RO monoclonal antibody (Product # 17-0457-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Functional expression of CD137 (4-1BB) on T helper follicular cells.

Oncoimmunology

Alfaro C,Echeveste JI,Rodriguez-Ruiz ME,Solorzano JL,Perez-Gracia JL,Idoate MA,Lopez-Picazo JM,Sanchez-Paulete AR,Labiano S,Rouzaut A,Oñate C,Aznar A,Lozano MD,Melero I

Published figure using CD45RO monoclonal antibody (Product # 17-0457-42) in Flow Cytometry

Tue Dec 01 00:00:00 EST 2015

Expression of the memory marker CD45RO on helper T cells in macaques.

PloS one

Valentine M,Song K,Maresh GA,Mack H,Huaman MC,Polacino P,Ho O,Cristillo A,Kyung Chung H,Hu SL,Pincus SH

17-0457 was used in Binding experiments to identify and eliminate subsets of T cells known to house a latent reservoir of HIV.

Tue Apr 08 00:00:00 EDT 2014

Expression of the memory marker CD45RO on helper T cells in macaques.

PloS one

Valentine M,Song K,Maresh GA,Mack H,Huaman MC,Polacino P,Ho O,Cristillo A,Kyung Chung H,Hu SL,Pincus SH

17-0457 was used in Binding experiments to identify and eliminate subsets of T cells known to house a latent reservoir of HIV.

Tue Apr 08 00:00:00 EDT 2014

Galectin-9 activates and expands human T-helper 1 cells.

PloS one

Gooden MJ,Wiersma VR,Samplonius DF,Gerssen J,van Ginkel RJ,Nijman HW,Hirashima M,Niki T,Eggleton P,Helfrich W,Bremer E

17-0457 was used in Flow cytometry/Cell sorting to demonstrate that galectin-9 activates resting T-cells and promotes their transition to a T-helper 1 cell phenotype.

Fri Jan 10 00:00:00 EST 2014

RUNX transcription factor-mediated association of Cd4 and Cd8 enables coordinate gene regulation.

Immunity

Collins A,Hewitt SL,Chaumeil J,Sellars M,Micsinai M,Allinne J,Parisi F,Nora EP,Bolland DJ,Corcoran AE,Kluger Y,Bosselut R,Ellmeier W,Chong MM,Littman DR,Skok JA

17-0457 was used in Flow cytometry/Cell sorting to study how communication between Cd4 and Cd8 appears to enable long-range epigenetic regulation to ensure that expression of one excludes the other in mature CD4 or CD8 single-positive cells.

Fri Mar 25 00:00:00 EDT 2011

RUNX transcription factor-mediated association of Cd4 and Cd8 enables coordinate gene regulation.

Immunity

Collins A,Hewitt SL,Chaumeil J,Sellars M,Micsinai M,Allinne J,Parisi F,Nora EP,Bolland DJ,Corcoran AE,Kluger Y,Bosselut R,Ellmeier W,Chong MM,Littman DR,Skok JA

Fri Mar 25 00:00:00 EDT 2011

Differential expression of CD300a/c on human TH1 and TH17 cells.

BMC immunology

Simhadri VR,Mariano JL,Zhou Q,DeBell KE,Borrego F

17-0457 was used in Flow cytometry/Cell sorting to conclude that the CD300a/c receptors are differentially expressed on human TH1 and TH17 cells, and that their ligation is capable of modulating TCR mediated signals.

Wed Nov 02 00:00:00 EDT 2011

Human Th1 cells that express CD300a are polyfunctional and after stimulation up-regulate the T-box transcription factor eomesodermin.

PloS one

Narayanan S,Silva R,Peruzzi G,Alvarez Y,Simhadri VR,Debell K,Coligan JE,Borrego F

17-0457 was used in Flow cytometry/Cell sorting to investigate a subset within the CD4 T cell population defined by expression of the immunomodulatory receptor CD300a.

Thu May 13 00:00:00 EDT 2010

Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Nagata S,Ise T,Pastan I

17-0457 was used in Flow cytometry/Cell sorting to study the effects of Foxp3 and IL-2 on FCRL3-/+ nTreg cell subsets.

Mon Jun 15 00:00:00 EDT 2009

Bioinformatics

Protein Aliases: CD45; CD45 antigen; L-CA; Leukocyte common antigen; protein tyrosine phosphatase, receptor type, C; protein tyrosine phosphatase, receptor type, c polypeptide; Receptor-type tyrosine-protein phosphatase C; T200; T200 glycoprotein; T200 leukocyte common antigen

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Gene Aliases: B220; CD45; CD45R; GP180; L-CA; LCA; LY5; PTPRC; T200

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UniProt ID: (Human) P08575

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Entrez Gene ID: (Human) 5788

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Function(s)

protein tyrosine phosphatase activity transmembrane receptor protein tyrosine phosphatase activity protein binding protein kinase binding protein phosphatase protein modifying enzyme

Process(es)

negative regulation of T cell mediated cytotoxicity negative regulation of cytokine-mediated signaling pathway hematopoietic progenitor cell differentiation immunoglobulin biosynthetic process negative regulation of protein kinase activity protein dephosphorylation negative regulation of cell adhesion involved in substrate-bound cell migration cell surface receptor signaling pathway dephosphorylation T cell differentiation positive regulation of B cell proliferation peptidyl-tyrosine dephosphorylation B cell proliferation positive regulation of T cell proliferation cell cycle phase transition positive regulation of protein kinase activity bone marrow development stem cell development T cell receptor signaling pathway B cell receptor signaling pathway positive regulation of antigen receptor-mediated signaling pathway release of sequestered calcium ion into cytosol defense response to virus regulation of cell cycle positive regulation of hematopoietic stem cell migration positive regulation of stem cell proliferation

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CD45RO Monoclonal Antibody (UCHL1), APC, eBioscience™

CD45RO Antibody (17-0457-42) in Flow

Product Details

17-0457-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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