Invitrogen

CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), Functional Grade, eBioscience™

15 Published Figures

25 References

View all (173) ICAM-1 (CD54) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (15)

CD54 (ICAM-1) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 16

CD54 (ICAM-1) Antibody (16-0541-81) in Flow

Staining of mouse splenocytes with Anti-Mouse CD54 (ICAM-1) FITC (left) or PE (right). Autofluorescence is shown via open histogram. Total viable cells were used for analysis.

CD54 (ICAM-1) Antibody in Immunocytochemistry (ICC/IF)

CD54 (ICAM-1) Antibody in Western Blot (WB)

CD54 (ICAM-1) Antibody in Neutralization (Neu)

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Product Details

16-0541-81

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunohistochemistry (IHC)

View 2 publications 2 publications

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.5 µg/test

View 13 publications 13 publications

Neutralization (Neu)

Assay-Dependent

View 5 publications 5 publications

Functional Assay (Functional)

Assay-Dependent

View 1 publication 1 publication

Inhibition Assays (IA)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), Functional Grade, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

YN1/1.7.4

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_468978

Product Specific Information

Description: The YN1/1.7.4 monoclonal antibody reacts with mouse CD54 (InterCellular Adhesion Molecule-1, ICAM-1), a 90-110 kDa transmembrane glycoprotein expressed by monocytes, lymphocytes, dendritic cells, and endothelial cells. Expression of CD54 is upregulated on activated lymphocytes. Interaction of CD54 with its ligands CD11a and CD11b is important in the inflammatory response. The YN1/1.7.4 monoclonal antibody recognizes a different epitope than the eBioKAT-1 monclonal antibody.

Applications Reported: The YN1/1.7.4 antibody has been reported for use in flow cytometric analysis. YN1/1.7.4 has also been reported in blocking of CD54 in functional studies.

Applications Tested: The YN1/1.7.4 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

ICAM-1 (CD54) is an 85-110 kDa single-chain type 1 integral membrane glycoprotein with an extracellular domain of five immunoglobulin superfamily repeats, a transmembrane region and a cytoplasmic domain. ICAM-1 has 7 potential N-linked glycosylation sites and shares considerable amino acid sequence homology with ICAM-3 (CD50) and ICAM-2 (CD102). ICAM-1 binds to integrins of type CD11a/CD18 (leukocyte adhesion molecule, LFA-1), or CD11b/CD18 (Mac-1) and is exploited by Rhinovirus as a receptor. ICAM-1 is expressed by activated endothelial cells and detected on epithelial cells, fibroblasts, chondrocytes, B lymphocytes, T lymphocytes (low), monocytes, macrophages, dendritic cells and neutrophils, with lower levels that increase upon inflammation. ICAM-1 is also detected in some carcinoma and melanoma cells. Soluble ICAM-1 is detectable in the plasma and is elevated in patients with various inflammatory syndromes.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

A Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Assay Identifies Nilotinib as an Inhibitor of Inflammation in Acute Myeloid Leukemia.

Journal of medicinal chemistry

Marín-Rubio JL,Peltier-Heap RE,Dueñas ME,Heunis T,Dannoura A,Inns J,Scott J,Simpson AJ,Blair HJ,Heidenreich O,Allan JM,Watt JE,Martin MP,Saxty B,Trost M

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0541-81) in Flow Cytometry

Thu Sep 22 00:00:00 EDT 2022

Macrophage ICAM-1 functions as a regulator of phagocytosis in LPS induced endotoxemia.

Inflammation research : official journal of the European Histamine Research Society ... [et al.]

Zhong H,Lin H,Pang Q,Zhuang J,Liu X,Li X,Liu J,Tang J

16-0541 was used in Neutralization experiments to demonstrate that ICAM-1-mediated macrophage phagocytosis depends on TLR4-mediated ROS production.

Mon Feb 01 00:00:00 EST 2021

ICAM-1 and ICAM-2 Are Differentially Expressed and Up-Regulated on Inflamed Pulmonary Epithelium, but Neither ICAM-2 nor LFA-1: ICAM-1 Are Required for Neutrophil Migration Into the Airways In Vivo.

Frontiers in immunology

Chong DLW,Rebeyrol C,José RJ,Williams AE,Brown JS,Scotton CJ,Porter JC

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0541-81) in Immunohistochemistry

Mon Oct 25 00:00:00 EDT 2021

Conserved regulation of RNA processing in somatic cell reprogramming.

BMC genomics

Kanitz A,Syed AP,Kaji K,Zavolan M

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0541-81) in Flow Cytometry

Thu Jan 31 00:00:00 EST 2019

VCAM-1 Density and Tumor Perfusion Predict T-cell Infiltration and Treatment Response in Preclinical Models.

Neoplasia (New York, N.Y.)

Riegler J,Gill H,Ogasawara A,Hedehus M,Javinal V,Oeh J,Ferl GZ,Marik J,Williams S,Sampath D,Schartner J,Carano RAD

16-0541 was used in Flow cytometry/Cell sorting to test if non-invasive imaging of tumor perfusion and vascular inflammation may be able to explain differences in T-cell infiltration in pre-clinical tumor models, relevant for treatment outcomes.

Tue Oct 01 00:00:00 EDT 2019

PAI1 mediates fibroblast-mast cell interactions in skin fibrosis.

The Journal of clinical investigation

Pincha N,Hajam EY,Badarinath K,Batta SPR,Masudi T,Dey R,Andreasen P,Kawakami T,Samuel R,George R,Danda D,Jacob PM,Jamora C

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0541-81) in Neutralization

Tue May 01 00:00:00 EDT 2018

Low-Dose Radiotherapy Has No Harmful Effects on Key Cells of Healthy Non-Inflamed Joints.

International journal of molecular sciences

Deloch L,Rückert M,Fietkau R,Frey B,Gaipl US

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0541-81) in Flow Cytometry

Tue Oct 16 00:00:00 EDT 2018

Fine-Tuning Mybl2 Is Required for Proper Mesenchymal-to-Epithelial Transition during Somatic Reprogramming.

Cell reports

Ward C,Volpe G,Cauchy P,Ptasinska A,Almaghrabi R,Blakemore D,Nafria M,Kestner D,Frampton J,Murphy G,Buganim Y,Kaji K,García P

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0541-81) in Flow Cytometry

Tue Aug 07 00:00:00 EDT 2018

Constitutively Active SMAD2/3 Are Broad-Scope Potentiators of Transcription-Factor-Mediated Cellular Reprogramming.

Cell stem cell

Ruetz T,Pfisterer U,Di Stefano B,Ashmore J,Beniazza M,Tian TV,Kaemena DF,Tosti L,Tan W,Manning JR,Chantzoura E,Ottosson DR,Collombet S,Johnsson A,Cohen E,Yusa K,Linnarsson S,Graf T,Parmar M,Kaji K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0541-81) in Flow Cytometry

Thu Dec 07 00:00:00 EST 2017

Fibroblastic reticular cells of the lymphoid tissues modulate T cell activation threshold during homeostasis via hyperactive cyclooxygenase-2/prostaglandin E₂ axis.

Scientific reports

Yu M,Guo G,Zhang X,Li L,Yang W,Bollag R,Cui Y

16-0541 was used in Flow cytometry/Cell sorting to identify an intrinsic mechanism of fibroblastic reticular cells shared between mice and humans for suppressing T cell sensitivity to activation via PGE2.

Tue Jun 13 00:00:00 EDT 2017

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

16-0541 was used in Blocking experiments to studying Treg cell engagement with the lymphotoxin beta receptor in transendothelial migration.

Tue Jun 21 00:00:00 EDT 2016

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

16-0541 was used in Blocking experiments to studying Treg cell engagement with the lymphotoxin beta receptor in transendothelial migration.

Tue Jun 21 00:00:00 EDT 2016

Treg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration.

Nature communications

Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

16-0541 was used in Blocking experiments to studying Treg cell engagement with the lymphotoxin beta receptor in transendothelial migration.

Tue Jun 21 00:00:00 EDT 2016

Palmitoyl acyltransferase DHHC21 mediates endothelial dysfunction in systemic inflammatory response syndrome.

Nature communications

Beard RS,Yang X,Meegan JE,Overstreet JW,Yang CG,Elliott JA,Reynolds JJ,Cha BJ,Pivetti CD,Mitchell DA,Wu MH,Deschenes RJ,Yuan SY

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0541-81) in Immunofluorescence

Thu Sep 22 00:00:00 EDT 2016

Palmitoyl acyltransferase DHHC21 mediates endothelial dysfunction in systemic inflammatory response syndrome.

Nature communications

Beard RS,Yang X,Meegan JE,Overstreet JW,Yang CG,Elliott JA,Reynolds JJ,Cha BJ,Pivetti CD,Mitchell DA,Wu MH,Deschenes RJ,Yuan SY

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0541-81) in Immunofluorescence

Thu Sep 22 00:00:00 EDT 2016

Ectonucleotidase activity and immunosuppression in astrocyte-CD4 T cell bidirectional signaling.

Oncotarget

Filipello F,Pozzi D,Proietti M,Romagnani A,Mazzitelli S,Matteoli M,Verderio C,Grassi F

16-0541 was used in Flow cytometry/Cell sorting to investigate how TCR-independent interactions promote an immunosuppressive program in T cells to control possible brain injury by deregulated T cell activation during neuroinflammation.

Tue Feb 02 00:00:00 EST 2016

Intercellular adhesion molecule-1 expression by skeletal muscle cells augments myogenesis.

Experimental cell research

Goh Q,Dearth CL,Corbett JT,Pierre P,Chadee DN,Pizza FX

16-0541 was used in Neutralization experiments to reveal mechanisms through which skeletal muscle cell expression of ICAM-1 augments the regenerative and hypertrophic processes of myogenesis.

Sun Feb 15 00:00:00 EST 2015

Reprogramming Roadblocks Are System Dependent.

Stem cell reports

Chantzoura E,Skylaki S,Menendez S,Kim SI,Johnsson A,Linnarsson S,Woltjen K,Chambers I,Kaji K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0541-81) in Flow Cytometry

Tue Sep 08 00:00:00 EDT 2015

Natural killer T cells are essential for the development of contact hypersensitivity in BALB/c mice.

The Journal of investigative dermatology

Shimizuhira C,Otsuka A,Honda T,Kitoh A,Egawa G,Nakajima S,Nakashima C,Watarai H,Miyachi Y,Kabashima K

16-0541 was used in Flow cytometry/Cell sorting to report the pivotal contribution of dendritic and natural killer T cell interactions to hypersensitivity.

Sat Nov 01 00:00:00 EDT 2014

Dietary gluten increases natural killer cell cytotoxicity and cytokine secretion.

European journal of immunology

Larsen J,Dall M,Antvorskov JC,Weile C,Engkilde K,Josefsen K,Buschard K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0541-81) in Flow Cytometry

Wed Oct 01 00:00:00 EDT 2014

High-resolution analysis with novel cell-surface markers identifies routes to iPS cells.

Nature

O'Malley J,Skylaki S,Iwabuchi KA,Chantzoura E,Ruetz T,Johnsson A,Tomlinson SR,Linnarsson S,Kaji K

Published figure using CD54 (ICAM-1) monoclonal antibody (Product # 16-0541-81) in Flow Cytometry

Thu Jul 04 00:00:00 EDT 2013

ICAM-1-dependent homotypic aggregates regulate CD8 T cell effector function and differentiation during T cell activation.

Journal of immunology (Baltimore, Md. : 1950)

Zumwalde NA,Domae E,Mescher MF,Shimizu Y

16-0541 was used in Flow cytometry/Cell sorting to demonstrate that during CD8 T cell activation, the T cell clustering mechanism allows for continued proliferation of T cells, and regulates CD8 T cell effector function and differentiation.

Tue Oct 01 00:00:00 EDT 2013

Lung-derived mesenchymal stromal cell post-transplantation survival, persistence, paracrine expression, and repair of elastase-injured lung.

Stem cells and development

Hoffman AM,Paxson JA,Mazan MR,Davis AM,Tyagi S,Murthy S,Ingenito EP

16-0541 was used in Blocking experiments to examine how lung-derived MSCs differ from syngeneic bone marrow MSCs in vitro and after transplantation in a murine model of emphysema.

Sat Oct 01 00:00:00 EDT 2011

Crosstalk between CXCR4/stromal derived factor-1 and VLA-4/VCAM-1 pathways regulates neutrophil retention in the bone marrow.

Journal of immunology (Baltimore, Md. : 1950)

Petty JM,Lenox CC,Weiss DJ,Poynter ME,Suratt BT

16-0541 was used in Neutralization experiments to show that alpha(4)beta(1) integrin is expressed on murine marrow neutrophils, decreases with maturation and its blockade leads to the release of marrow neutrophils.

Thu Jan 01 00:00:00 EST 2009

Targeted delivery of oligodeoxynucleotides to mouse lung endothelial cells in vitro and in vivo.

Molecular therapy : the journal of the American Society of Gene Therapy

Wilson A,Zhou W,Champion HC,Alber S,Tang ZL,Kennel S,Watkins S,Huang L,Pitt B,Li S

16-0541 was used in Immunohistochemistry-immunofluorescence to develop a novel lipidic vector that is highly efficient in targeted delivery of oligodeoxynucleotides to pulmonary endothelium.

Thu Sep 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: CD54; Human rhinovirus receptor; ICAM-1; Intercellular adhesion molecule 1; Ly 47; MALA-2; MALA2; MyD10; sCD54; soluble CD54; Surface antigen of activated B cells

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Gene Aliases: CD54; Icam-1; Icam1; Ly-47; MALA-2

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UniProt ID: (Mouse) P13597

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Entrez Gene ID: (Mouse) 15894

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Function(s)

integrin binding protein complex binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD54 (ICAM-1) Monoclonal Antibody (YN1/1.7.4), Functional Grade, eBioscience™

CD54 (ICAM-1) Antibody (16-0541-81) in Flow

Product Details

16-0541-81

Western Blot (WB)

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Neutralization (Neu)

Functional Assay (Functional)

Inhibition Assays (IA)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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