Invitrogen

CD57 Monoclonal Antibody (TB01 (TBO1)), Functional Grade, eBioscience™

6 Published Figures

12 References

View all (39) CD57 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Published Figures (6)

CD57 Antibody in Immunocytochemistry, Flow Cytometry (ICC/IF, Flow)

FIGURE: 1 / 6

CD57 Antibody (16-0577-85) in ICC/IF, Flow

Autophagy 2012 - Figure 6. Levels of gammaH2AX foci in senescent T cells with low autophagy. PBMCs from three healthy donors were bead sorted for CD8 + T cells and stained for CD57 gammaH2AX, LC3 and Lyso-ID and run on ImageStream. (A) Representative BDS overlay histogram for CD8 + CD57 + (red) for CD8 + CD57 - (green) depicts the low and high autophagy gates. (B) Bar graph showing proportion of CD8 + CD57 +/- lymphocytes w... View More

CD57 Antibody in Immunohistochemistry, Flow Cytometry (IHC, Flow)

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Product Details

16-0577-85

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

1 µg/test

View 10 publications 10 publications

Functional Assay (Functional)

Assay-Dependent

Control (Ctrl)

Assay-Dependent

Product Specifications

Species Reactivity

Human

Published species

Human

Host/Isotype

Mouse / IgM

Class

Monoclonal

Type

Antibody

Clone

TB01 (TBO1)

Conjugate

Functional Grade Functional Grade Functional Grade

Form

Liquid

Concentration

1 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

no preservative

Storage conditions

4° C

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1944388

Product Specific Information

Description: This TB01 monoclonal antibody reacts with human CD57 (also known as HNK-1 and Leu-7), a 110-kDa cell surface glycoprotein expressed on a subset of natural killer (NK) cells and NK T cells.

Applications Reported: This TB01 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This TB01 (TBO1) antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

Target Information

CD57, also known as HNK-1 and Leu-7, is an oligosaccharide antigenic determinant present on a variety of polypeptides, lipids, and chondroitin sulphate proteoglycans. Its function is poorly understood. CD57 is expressed on a subset of natural killer (NK) cells and NK T cells.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Aberrant newborn T cell and microbiota developmental trajectories predict respiratory compromise during infancy.

iScience

McDavid A,Laniewski N,Grier A,Gill AL,Kessler HA,Huyck H,Carbonell E,Holden-Wiltse J,Bandyopadhyay S,Carnahan J,Dylag AM,Topham DJ,Falsey AR,Caserta MT,Pryhuber GS,Gill SR,Scheible KM

Published figure using CD57 monoclonal antibody (Product # 16-0577-85) in Flow Cytometry

Fri Apr 15 00:00:00 EDT 2022

Phenotypic analysis of the unstimulated in vivo HIV CD4 T cell reservoir.

eLife

Neidleman J,Luo X,Frouard J,Xie G,Hsiao F,Ma T,Morcilla V,Lee A,Telwatte S,Thomas R,Tamaki W,Wheeler B,Hoh R,Somsouk M,Vohra P,Milush J,James KS,Archin NM,Hunt PW,Deeks SG,Yukl SA,Palmer S,Greene WC,Roan NR

Published figure using CD57 monoclonal antibody (Product # 16-0577-85) in Flow Cytometry

Tue Sep 29 00:00:00 EDT 2020

Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells.

Nature communications

Marquardt N,Kekäläinen E,Chen P,Lourda M,Wilson JN,Scharenberg M,Bergman P,Al-Ameri M,Hård J,Mold JE,Ljunggren HG,Michaëlsson J

16-0577 was used in Flow cytometry/Cell sorting to indicate that human lung trNK cells have distinct features, likely regulating their function in barrier immunity.

Mon Aug 26 00:00:00 EDT 2019

Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells.

Nature communications

Marquardt N,Kekäläinen E,Chen P,Lourda M,Wilson JN,Scharenberg M,Bergman P,Al-Ameri M,Hård J,Mold JE,Ljunggren HG,Michaëlsson J

16-0577 was used in Flow cytometry/Cell sorting to indicate that human lung trNK cells have distinct features, likely regulating their function in barrier immunity.

Mon Aug 26 00:00:00 EDT 2019

Tumor- and cytokine-primed human natural killer cells exhibit distinct phenotypic and transcriptional signatures.

PloS one

Sabry M,Zubiak A,Hood SP,Simmonds P,Arellano-Ballestero H,Cournoyer E,Mashar M,Pockley AG,Lowdell MW

16-0577-85 was used in Flow Cytometry to study the consequences of co-incubation with K562, CTV-1, Daudi RPMI-8226, and MCF-7 tumor cell lines on the phenotype, cytokine expression profile, and transcriptome of human natural killer cells.

Mon Feb 24 00:00:00 EST 2020

TCF1 expression marks self-renewing human CD8⁺ T cells.

Blood advances

Kratchmarov R,Magun AM,Reiner SL

16-0577 was used in Flow cytometry/Cell sorting to show analyses of TCF1 expression from patient specimens may become a useful biomarker for adaptive immune function in response to vaccination, infection, autoimmunity, and cancer.

Tue Jul 24 00:00:00 EDT 2018

Composition and dynamics of the uterine NK cell KIR repertoire in menstrual blood.

Mucosal immunology

Ivarsson MA,Stiglund N,Marquardt N,Westgren M,Gidlöf S,Björkström NK

16-0577 was used in Flow cytometry/Cell sorting to provide novel insight into KIR repertoire formation on human uterine NK cells.

Wed Mar 01 00:00:00 EST 2017

Mass Cytometric Analysis of HIV Entry, Replication, and Remodeling in Tissue CD4+ T Cells.

Cell reports

Cavrois M,Banerjee T,Mukherjee G,Raman N,Hussien R,Rodriguez BA,Vasquez J,Spitzer MH,Lazarus NH,Jones JJ,Ochsenbauer C,McCune JM,Butcher EC,Arvin AM,Sen N,Greene WC,Roan NR

Published figure using CD57 monoclonal antibody (Product # 16-0577-85) in Immunofluorescence

Tue Jul 25 00:00:00 EDT 2017

Mass Cytometric Analysis of HIV Entry, Replication, and Remodeling in Tissue CD4+ T Cells.

Cell reports

Cavrois M,Banerjee T,Mukherjee G,Raman N,Hussien R,Rodriguez BA,Vasquez J,Spitzer MH,Lazarus NH,Jones JJ,Ochsenbauer C,McCune JM,Butcher EC,Arvin AM,Sen N,Greene WC,Roan NR

Published figure using CD57 monoclonal antibody (Product # 16-0577-85) in Immunofluorescence

Tue Jul 25 00:00:00 EDT 2017

The Human NK Cell Response to Yellow Fever Virus 17D Is Primarily Governed by NK Cell Differentiation Independently of NK Cell Education.

Journal of immunology (Baltimore, Md. : 1950)

Marquardt N,Ivarsson MA,Blom K,Gonzalez VD,Braun M,Falconer K,Gustafsson R,Fogdell-Hahn A,Sandberg JK,Michaëlsson J

16-0577 was used in Flow cytometry/Cell sorting to study the temporal dynamics and regulation of natural killer cell responses in an acute viral infection.

Thu Oct 01 00:00:00 EDT 2015

Cutting edge: identification and characterization of human intrahepatic CD49a+ NK cells.

Journal of immunology (Baltimore, Md. : 1950)

Marquardt N,Béziat V,Nyström S,Hengst J,Ivarsson MA,Kekäläinen E,Johansson H,Mjösberg J,Westgren M,Lankisch TO,Wedemeyer H,Ellis EC,Ljunggren HG,Michaëlsson J,Björkström NK

16-0577-85 was used in Flow Cytometry to study the unique lineage of CD49a(+)DX5(-) NK cells, showing that they are readily detectable in the liver but not in hepatic venous or peripheral blood.

Sun Mar 15 00:00:00 EDT 2015

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

Published figure using CD57 monoclonal antibody (Product # 16-0577-85) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

Published figure using CD57 monoclonal antibody (Product # 16-0577-85) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Bioinformatics

Protein Aliases: Beta-1,3-glucuronyltransferase 1; Galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase 1; GlcAT-P; GlcUAT-P; Glucuronosyltransferase P; HNK-1; Leu-7; LEU7 antigen; UDP-GlcUA:glycoprotein beta-1,3-glucuronyltransferase

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Gene Aliases: B3GAT1; CD57; GLCATP; GLCUATP; HNK1; LEU7; NK-1; NK1

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UniProt ID: (Human) Q9P2W7

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Entrez Gene ID: (Human) 27087

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Function(s)

galactosylgalactosylxylosylprotein 3-beta-glucuronosyltransferase activity metal ion binding glycosyltransferase

Process(es)

carbohydrate metabolic process protein glycosylation glycosaminoglycan metabolic process chondroitin sulfate metabolic process chondroitin sulfate proteoglycan biosynthetic process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD57 Monoclonal Antibody (TB01 (TBO1)), Functional Grade, eBioscience™

CD57 Antibody (16-0577-85) in ICC/IF, Flow

Product Details

16-0577-85

Immunohistochemistry (IHC)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Functional Assay (Functional)

Control (Ctrl)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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