Invitrogen

CD8 Monoclonal Antibody (3B5), APC

1 Published Figure

16 References

View all (532) CD8 antibodies

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FIGURE: 1 / 2

CD8 Antibody (MHCD0805) in Flow

Human peripheral blood lymphocytes were stained using APC of anti-human CD8 monoclonal antibody (clone 3B5). The negative control profiles represent unstained cells.

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Product Details

MHCD0805

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 10 publications 10 publications

Functional Assay (Functional)

View 1 publication 1 publication

Miscellaneous PubMed (Misc)

View 5 publications 5 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Non-human primate

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

3B5

Immunogen

Human CD8

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with BSA, sucrose

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10392701

Product Specific Information

Allophycocyanin (APC) is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients.

This antibody recognizes the alpha chain alone as well as the alpha/beta heterodimer.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

Analyte Specific Reagent

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Product References

Biochemical and functional characterization of mutant KRAS epitopes validates this oncoprotein for immunological targeting.

Nature communications

Bear AS,Blanchard T,Cesare J,Ford MJ,Richman LP,Xu C,Baroja ML,McCuaig S,Costeas C,Gabunia K,Scholler J,Posey AD,O'Hara MH,Smole A,Powell DJ,Garcia BA,Vonderheide RH,Linette GP,Carreno BM

MHCD0805 was used in Flow Cytometry to characterize HLA class I-restricted mutated KRAS epitopes in cancer.

Fri Jul 16 00:00:00 EDT 2021

High-Throughput Generation of Bipod (Fab × scFv) Bispecific Antibodies Exploits Differential Chain Expression and Affinity Capture.

Scientific reports

Nesspor TC,Kinealy K,Mazzanti N,Diem MD,Boye K,Hoffman H,Springer C,Sprenkle J,Powers G,Jiang H,La Porte SL,Ganesan R,Singh S,Zwolak A

MHCD0805 was used in Functional assays to describe a high-throughput method for generation of Bipods based on protein A and CH1 domain affinity capture.

Tue May 05 00:00:00 EDT 2020

High-Throughput Generation of Bipod (Fab × scFv) Bispecific Antibodies Exploits Differential Chain Expression and Affinity Capture.

Scientific reports

Nesspor TC,Kinealy K,Mazzanti N,Diem MD,Boye K,Hoffman H,Springer C,Sprenkle J,Powers G,Jiang H,La Porte SL,Ganesan R,Singh S,Zwolak A

MHCD0805 was used in Functional assays to describe a high-throughput method for generation of Bipods based on protein A and CH1 domain affinity capture.

Tue May 05 00:00:00 EDT 2020

Methodology for in vitro Assessment of Human T Cell Activation and Blockade.

Bio-protocol

Podojil JR,Ifergan I,Chiang MY,Meeks JJ,Miller SD

MHCD0805 was used in Flow Cytometry to assess in vitro functionality and mechanism for action in human T cells.

Fri Jun 05 00:00:00 EDT 2020

Human CD4⁺ T Cells Specific for Merkel Cell Polyomavirus Localize to Merkel Cell Carcinomas and Target a Required Oncogenic Domain.

Cancer immunology research

Longino NV,Yang J,Iyer JG,Ibrani D,Chow IT,Laing KJ,Campbell VL,Paulson KG,Kulikauskas RM,Church CD,James EA,Nghiem P,Kwok WW,Koelle DM

MHCD0805 was used in Flow cytometry/Cell sorting to report the identification of CD4+ T-cell responses against six MCPyV epitopes, one of which included a conserved, essential viral oncogenic domain that binds/disables the cellular retinoblastoma (Rb) tumor suppressor.

Tue Oct 01 00:00:00 EDT 2019

Immunological Differences between Lymphocytic and Collagenous Colitis.

Journal of Crohn's & colitis

Carrasco A,Esteve M,Salas A,Pedrosa E,Rosinach M,Aceituno M,Zabana Y,Fernández-Bañares F

MHCD0805 was used in flow cytometry to determine mucosal characteristics of lymphocytic and collagenous colitis

Thu Sep 01 00:00:00 EDT 2016

Regional Specialisation of T Cell Subsets and Apoptosis in the Human Gut Mucosa: Differences Between Ileum and Colon in Healthy Intestine and Inflammatory Bowel Diseases.

Journal of Crohn's & colitis

Carrasco A,Fernández-Bañares F,Pedrosa E,Salas A,Loras C,Rosinach M,Aceituno M,Andújar X,Forné M,Zabana Y,Esteve M

MHCD0805 was used in flow cytometry to compare the immune compartmentalization between ileum and colon in healthy and inflamed mucosa

Thu Sep 01 00:00:00 EDT 2016

IL-12-secreting CD19-targeted cord blood-derived T cells for the immunotherapy of B-cell acute lymphoblastic leukemia.

Leukemia

Pegram HJ,Purdon TJ,van Leeuwen DG,Curran KJ,Giralt SA,Barker JN,Brentjens RJ

MHCD0805 was used in flow cytometry to developed a strategy to expand umbilical cord blood T cells and test their effects after transplantation

Sun Feb 01 00:00:00 EST 2015

Differential Immune Profiles in Two Pandemic Influenza A(H1N1)pdm09 Virus Waves at Pandemic Epicenter.

Archives of medical research

Arriaga-Pizano L,Ferat-Osorio E,Rodríguez-Abrego G,Mancilla-Herrera I,Domínguez-Cerezo E,Valero-Pacheco N,Pérez-Toledo M,Lozano-Patiño F,Laredo-Sánchez F,Malagón-Rangel J,Nellen-Hummel H,González-Bonilla C,Arteaga-Troncoso G,Cérbulo-Vázquez A,Pastelin-Palacios R,Klenerman P,Isibasi A,López-Macías C

MHCD0805 was used in Flow cytometry/Cell sorting to show that during the 2009 influenza pandemic a T cell activation phenotype is observed in a wave-dependent fashion.

Sun Nov 01 00:00:00 EDT 2015

T cell interleukin-15 surface expression in chimpanzees infected with human immunodeficiency virus.

Cellular immunology

Rodriguez AR,Hodara V,Murthy K,Morrow L,Sanchez M,Bienvenu AE,Murthy KK

MHCD0805 was used in flow cytometry to examine the role of IL-15 in HIV-infected chimpanzees

Thu May 29 00:00:00 EDT 2014

Human neonatal naive CD4+ T cells have enhanced activation-dependent signaling regulated by the microRNA miR-181a.

Journal of immunology (Baltimore, Md. : 1950)

Palin AC,Ramachandran V,Acharya S,Lewis DB

MHCD0805 was used in flow cytometry to test if impaired human neonatal CD4(+) T cell immunity is due to reduced signaling by naive CD4(+) T cells following engagement of the αβ-TCR/CD3 complex and CD28.

Fri Mar 15 00:00:00 EDT 2013

CD8+ lymphocytes do not mediate protection against acute superinfection 20 days after vaccination with a live attenuated simian immunodeficiency virus.

Journal of virology

Stebbings R,Berry N,Waldmann H,Bird P,Hale G,Stott J,North D,Hull R,Hall J,Lines J,Brown S,D'Arcy N,Davis L,Elsley W,Edwards C,Ferguson D,Allen J,Almond N

MHCD0805 was used in Flow cytometry/Cell sorting to study whether CD8+ cytotoxic T lymphocytes mediate protection against acute superinfection.

Sat Oct 01 00:00:00 EDT 2005

Phenotypic and molecular characterization of CD103+ CD4+ T cells in bronchoalveolar lavage from patients with interstitial lung diseases.

Cytometry. Part B, Clinical cytometry

Braun RK,Foerster M,Grahmann PR,Haefner D,Workalemahu G,Kroegel C

MHCD0805 was used in flow cytometry to test if CD103 has a regulatory role in the microenvironment of the epithelial cell layer.

Tue Jul 01 00:00:00 EDT 2003

Reduced cell surface expression of CCR5 in CCR5Delta 32 heterozygotes is mediated by gene dosage, rather than by receptor sequestration.

The Journal of biological chemistry

Venkatesan S,Petrovic A,Van Ryk DI,Locati M,Weissman D,Murphy PM

MHCD0805 was used in flow cytometry to determine how CCR5 expression is reduced in cells from ccr5Delta32 heterozygotes.

Fri Jan 18 00:00:00 EST 2002

A membrane-proximal basic domain and cysteine cluster in the C-terminal tail of CCR5 constitute a bipartite motif critical for cell surface expression.

The Journal of biological chemistry

Venkatesan S,Petrovic A,Locati M,Kim YO,Weissman D,Murphy PM

MHCD0805 was used in flow cytometry to characterize CCR5 truncations.

Fri Oct 26 00:00:00 EDT 2001

Quantification of CD4, CCR5, and CXCR4 levels on lymphocyte subsets, dendritic cells, and differentially conditioned monocyte-derived macrophages.

Proceedings of the National Academy of Sciences of the United States of America

Lee B,Sharron M,Montaner LJ,Weissman D,Doms RW

MHCD0805 was used in flow cytometry to determine the number of CD4, CCR5, and CXCR4 antibody-binding sites on various T cells and macrophages.

Tue Apr 27 00:00:00 EDT 1999

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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Function(s)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

T cell mediated immunity immune response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway antigen processing and presentation T cell activation cytotoxic T cell differentiation regulation of immune response positive regulation of calcium-mediated signaling defense response to virus adaptive immune response regulation of defense response to virus by virus

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8 Monoclonal Antibody (3B5), APC

CD8 Antibody (MHCD0805) in Flow

Product Details

MHCD0805

Flow Cytometry (Flow)

Functional Assay (Functional)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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