Invitrogen

CD8a Monoclonal Antibody (RPA-T8), APC-eFluor™ 780, eBioscience™

FIGURE: 1 / 41

CD8a Antibody (47-0088-42) in Flow

Staining of normal human peripheral blood cells with Anti-Human CD19 PE (Product # 12-0199-80) and Mouse IgG1 K Isotype Control APC-eFluor® 780 (Product # 47-4714-82) (left) or Anti-Human CD8a APC-eFluor® 780 (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

47-0088-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 35 publications 35 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse, Rat

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

RPA-T8

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1272046

Product Specific Information

Description: The RPA-T8 monoclonal antibody reacts with the human CD8a molecule, an approximately 32-34 kDa cell surface receptor expressed either as a heterodimer with the CD8 beta chain (CD8 alpha/beta) or as a homodimer (CD8 alpha/alpha). A majority of thymocytes and a subpopulation of mature T cells and NK cells express CD8a. CD8 binds to MHC class I and through its association with protein tyrosine kinase p56lck plays a role in T-cell development and activation of mature T cells.

Applications Reported: This RPA-T8 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This RPA-T8 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Cluster of differentiation 8 (CD8), a type I transmembrane glycoprotein of the immunoglobulin family of receptors, plays an integral role in signal transduction, and T cell differentiation and activation. CD8 is predominantly expressed on T cells as a disulfide-linked heterodimer of CD8alpha and CD8beta, where it functions as a co-receptor, along with T cell receptor (TCR), for major histocompatibilty complex class I (MHC-I) molecules; whereas its counterpart, CD4, acts as a co-receptor for MHC-II molecules. CD8 exists on the cell surface, where the CD8alpha chain is essential for binding to MHC-I. CD8 is also expressed on a subset of T cells, NK cells, monocytes and dendritic cells as disulfide-linked homodimers of CD8alpha. Ligation of MHC-I/peptide complexes presented by antigen-presenting cells (APCs), triggers the recruitment of lymphocyte-specific protein tyrosine kinase (Lck), which leads to lymphokine production, motility and cytotoxic T lymphocyte (CTL) activation. Once activated, CTLs play a crucial role in the clearance of pathogens and tumor cells. Differentiation of naive CD8+ T cells into CTLs is strongly enhanced by IL-2, IL-12 and TGF-beta1.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Inactivated whole-virion vaccine BBV152/Covaxin elicits robust cellular immune memory to SARS-CoV-2 and variants of concern.

Nature microbiology

Vikkurthi R,Ansari A,Pai AR,Jha SN,Sachan S,Pandit S,Nikam B,Kalia A,Jit BP,Parray HA,Singh S,Kshetrapal P,Wadhwa N,Shrivastava T,Coshic P,Kumar S,Sharma P,Sharma N,Taneja J,Pandey AK,Sharma A,Thiruvengadam R,Grifoni A,Weiskopf D,Sette A,Bhatnagar S,Gupta N

47-0088-42 was used in Flow cytometry/Cell sorting to show that inactivated vaccine BBV152 induces robust immune memory to SARS-CoV-2 and variants of concern that persists for at least 6 months after vaccination.

Fri Jul 01 00:00:00 EDT 2022

Monitoring the Sialome on Human Immune Cells.

Methods in molecular biology (Clifton, N.J.)

O'Farrell LK,Fraser AD,Davey GP

47-0088-42 was used in Flow cytometry/Cell sorting to indicate that regulating the sialome content on the surface of activated immune cells may be monitored during immunomodulatory responses and anti-inflammatory therapies.

Mon Dec 20 00:00:00 EST 2021

Severe T cell hyporeactivity in ventilated COVID-19 patients correlates with prolonged virus persistence and poor outcomes.

Nature communications

Renner K,Schwittay T,Chaabane S,Gottschling J,Müller C,Tiefenböck C,Salewski JN,Winter F,Buchtler S,Balam S,Malfertheiner MV,Lubnow M,Lunz D,Graf B,Hitzenbichler F,Hanses F,Poeck H,Kreutz M,Orsó E,Burkhardt R,Niedermair T,Brochhausen C,Gessner A,Salzberger B,Mack M

47-0088-42 was used in Flow Cytometry to develop a score to predict fatal outcomes and identify patients that may benefit from strategies to overcome T cell hyporeactivity.

Fri May 21 00:00:00 EDT 2021

Severe T cell hyporeactivity in ventilated COVID-19 patients correlates with prolonged virus persistence and poor outcomes.

Nature communications

47-0088-42 was used in Flow Cytometry to develop a score to predict fatal outcomes and identify patients that may benefit from strategies to overcome T cell hyporeactivity.

Fri May 21 00:00:00 EDT 2021

Multipeptide stimulated PBMCs generate T_EM/T_CM for adoptive cell therapy in multiple myeloma.

Oncotarget

Vardam-Kaur T,Pathangey LB,McCormick DJ,Bergsagel PL,Cohen PA,Gendler SJ

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Tue Sep 28 00:00:00 EDT 2021

Human endoglin-CD3 bispecific T cell engager antibody induces anti-tumor effect in vivo.

Theranostics

Zhong L,Shi W,Gan L,Liu X,Huo Y,Wu P,Zhang Z,Wu T,Peng H,Huang Y,Zhao Y,Yuan Y,Deng Z,Tang H

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Wed Jul 28 00:00:00 EDT 2021

Bispecific T-Cell Engaging Antibodies Against MUC16 Demonstrate Efficacy Against Ovarian Cancer in Monotherapy and in Combination With PD-1 and VEGF Inhibition.

Frontiers in immunology

Yeku OO,Rao TD,Laster I,Kononenko A,Purdon TJ,Wang P,Cui Z,Liu H,Brentjens RJ,Spriggs D

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Thu Oct 21 00:00:00 EDT 2021

Preventing ATP Degradation by ASO-Mediated Knockdown of CD39 and CD73 Results in A2aR-Independent Rescue of T Cell Proliferation.

Molecular therapy. Nucleic acids

Festag J,Thelemann T,Schell M,Raith S,Michel S,Jaschinski F,Klar R

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Fri Sep 04 00:00:00 EDT 2020

A Cross-Reactive Small Protein Binding Domain Provides a Model to Study Off-Tumor CAR-T Cell Toxicity.

Molecular therapy oncolytics

Hammill JA,Kwiecien JM,Dvorkin-Gheva A,Lau VWC,Baker C,Wu Y,Bezverbnaya K,Aarts C,Heslen CW,Denisova GF,Derocher H,Milne K,Nelson BH,Bramson JL

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Fri Jun 26 00:00:00 EDT 2020

Immunological Properties of Neural Crest Cells Derived from Human Induced Pluripotent Stem Cells.

Stem cells and development

Fujii S,Yoshida S,Inagaki E,Hatou S,Tsubota K,Takahashi M,Shimmura S,Sugita S

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2019

Absolute lymphocyte count and human adenovirus-specific T-cell immune restoration of human adenovirus infection after kidney transplantation.

Journal of medical virology

Bruminhent J,Apiwattanakul N,Hongeng S,Kantachuvesiri S,Watcharananan SP

47-0088-42 was used in Flow cytometry/Cell sorting to suggest a trend of ALC and HAdV-specific T-cell immune restoration in KT recipients who achieve successful HAdV clearance.

Thu Aug 01 00:00:00 EDT 2019

LIGHT-HVEM Signaling in Innate Lymphoid Cell Subsets Protects Against Enteric Bacterial Infection.

Cell host & microbe

Seo GY,Shui JW,Takahashi D,Song C,Wang Q,Kim K,Mikulski Z,Chandra S,Giles DA,Zahner S,Kim PH,Cheroutre H,Colonna M,Kronenberg M

47-0088-42 was used in Flow cytometry/Cell sorting to investigate if signaling through herpes virus entry mediator (HVEM) in Innate lymphoid cells 3 is important for host defense against oral infection with the bacterial pathogen Yersinia enterocolitica.

Wed Aug 08 00:00:00 EDT 2018

Effect of IL-7 Therapy on Phospho-Ribosomal Protein S6 and TRAF1 Expression in HIV-Specific CD8 T Cells in Patients Receiving Antiretroviral Therapy.

Journal of immunology (Baltimore, Md. : 1950)

Wang C,Edilova MI,Wagar LE,Mujib S,Singer M,Bernard NF,Croughs T,Lederman MM,Sereti I,Fischl MA,Kremmer E,Ostrowski M,Routy JP,Watts TH

47-0088 was used in Flow cytometry/Cell sorting to show that IL-7 therapy in humans increases the number of circulating HIV-specific CD8 T cells.

Mon Jan 15 00:00:00 EST 2018

The Genetic Polymorphisms of NLRP3 Inflammasome Associated with T Helper Cells in Patients with Multiple Myeloma.

Journal of immunology research

Zhao X,Hua M,Yan S,Yu J,Han F,Zhong C,Wang R,Zhang C,Hou M,Ma D

47-0088 was used in Flow cytometry/Cell sorting to investigate the role of the NLRP3 inflammasome in multiple myeloma.

Fri Nov 09 00:00:00 EST 2018

Activation-induced Markers Detect Vaccine-Specific CD4⁺ T Cell Responses Not Measured by Assays Conventionally Used in Clinical Trials.

Vaccines

Bowyer G,Rampling T,Powlson J,Morter R,Wright D,Hill AVS,Ewer KJ

47-0088 was used in Flow cytometry/Cell sorting to compare the use of markers for their sensitivity, specificity and associations with other measures of vaccine immunogenicity and demonstrate these as an additional method of reporting the T cell response to vaccination.

Tue Jul 31 00:00:00 EDT 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Safety and Immunogenicity of Malaria Vectored Vaccines Given with Routine Expanded Program on Immunization Vaccines in Gambian Infants and Neonates: A Randomized Controlled Trial.

Frontiers in immunology

Mensah VA,Roetynck S,Kanteh EK,Bowyer G,Ndaw A,Oko F,Bliss CM,Jagne YJ,Cortese R,Nicosia A,Roberts R,D'Alessio F,Leroy O,Faye B,Kampmann B,Cisse B,Bojang K,Gerry S,Viebig NK,Lawrie AM,Clarke E,Imoukhuede EB,Ewer KJ,Hill AVS,Afolabi MO

Mon Mar 14 00:00:00 EDT 2022

Detection of Retinal Pigment Epithelium-Specific Antibody in iPSC-Derived Retinal Pigment Epithelium Transplantation Models.

Stem cell reports

Sugita S,Makabe K,Fujii S,Iwasaki Y,Kamao H,Shiina T,Ogasawara K,Takahashi M

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Tue Nov 14 00:00:00 EST 2017

Early changes within the lymphocyte population are associated with the development of multiple organ dysfunction syndrome in trauma patients.

Critical care (London, England)

Manson J,Cole E,De'Ath HD,Vulliamy P,Meier U,Pennington D,Brohi K

47-0088 was used in Flow cytometry/Cell sorting to study how early changes within the lymphocyte population are associated with the development of multiple organ dysfunction syndrome in trauma patients.

Tue Jun 07 00:00:00 EDT 2016

CD57(+) CD4 T Cells Underlie Belatacept-Resistant Allograft Rejection.

American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons

Espinosa J,Herr F,Tharp G,Bosinger S,Song M,Farris AB,George R,Cheeseman J,Stempora L,Townsend R,Durrbach A,Kirk AD

47-0088 was used in Flow cytometry/Cell sorting to implicate CD57(+) CD4 T cells in clinical costimulation blockade-resistant rejection.

Fri Apr 01 00:00:00 EDT 2016

Follicular Regulatory CD8 T Cells Impair the Germinal Center Response in SIV and Ex Vivo HIV Infection.

PLoS pathogens

Miles B,Miller SM,Folkvord JM,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Sat Oct 01 00:00:00 EDT 2016

Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.

Journal of immunology (Baltimore, Md. : 1950)

Younis RH,Han KL,Webb TJ

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Umbilical Cord-Derived Mesenchymal Stem Cells Suppress Autophagy of T Cells in Patients with Systemic Lupus Erythematosus via Transfer of Mitochondria.

Stem cells international

Chen J,Wang Q,Feng X,Zhang Z,Geng L,Xu T,Wang D,Sun L

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Thu Oct 01 00:00:00 EDT 2020

CD28 family of receptors on T cells in chronic HBV infection: Expression characteristics, clinical significance and correlations with PD-1 blockade.

Molecular medicine reports

Tang ZS,Hao YH,Zhang EJ,Xu CL,Zhou Y,Zheng X,Yang DL

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Mon Aug 01 00:00:00 EDT 2016

Lack of T Cell Response to iPSC-Derived Retinal Pigment Epithelial Cells from HLA Homozygous Donors.

Stem cell reports

Sugita S,Iwasaki Y,Makabe K,Kimura T,Futagami T,Suegami S,Takahashi M

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Tue Oct 11 00:00:00 EDT 2016

CD103+ intraepithelial T cells in high-grade serous ovarian cancer are phenotypically diverse TCRαβ+ CD8αβ+ T cells that can be targeted for cancer immunotherapy.

Oncotarget

Komdeur FL,Wouters MC,Workel HH,Tijans AM,Terwindt AL,Brunekreeft KL,Plat A,Klip HG,Eggink FA,Leffers N,Helfrich W,Samplonius DF,Bremer E,Wisman GB,Daemen T,Duiker EW,Hollema H,Nijman HW,de Bruyn M

47-0088 was used in Flow cytometry/Cell sorting to determine whether CD103+ tumour-infiltrating lymphocytes are induced as part of an ongoing anti-high-grade serous epithelial ovarian cancer immune response.

Tue Nov 15 00:00:00 EST 2016

Liposomal Glutathione Supplementation Restores TH1 Cytokine Response to Mycobacterium tuberculosis Infection in HIV-Infected Individuals.

Journal of interferon & cytokine research : the official journal of the International Society for Interferon and Cytokine Research

Ly J,Lagman M,Saing T,Singh MK,Tudela EV,Morris D,Anderson J,Daliva J,Ochoa C,Patel N,Pearce D,Venketaraman V

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2015

Follicular regulatory T cells impair follicular T helper cells in HIV and SIV infection.

Nature communications

Miles B,Miller SM,Folkvord JM,Kimball A,Chamanian M,Meditz AL,Arends T,McCarter MD,Levy DN,Rakasz EG,Skinner PJ,Connick E

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Tue Oct 20 00:00:00 EDT 2015

RSV-specific airway resident memory CD8+ T cells and differential disease severity after experimental human infection.

Nature communications

Jozwik A,Habibi MS,Paras A,Zhu J,Guvenel A,Dhariwal J,Almond M,Wong EHC,Sykes A,Maybeno M,Del Rosario J,Trujillo-Torralbo MB,Mallia P,Sidney J,Peters B,Kon OM,Sette A,Johnston SL,Openshaw PJ,Chiu C

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Mon Dec 21 00:00:00 EST 2015

Investigating the causes for decreased levels of glutathione in individuals with type II diabetes.

PloS one

Lagman M,Ly J,Saing T,Kaur Singh M,Vera Tudela E,Morris D,Chi PT,Ochoa C,Sathananthan A,Venketaraman V

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Wed Dec 30 00:00:00 EST 2015

Immune response to JC virus T antigen in patients with and without colorectal neoplasia.

Gut microbes

Butcher LD,Garcia M,Arnold M,Ueno H,Goel A,Boland CR

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Tue Jul 01 00:00:00 EDT 2014

CD4+CD25(high), CD8+CD28- cells and thyroid autoantibodies in breast cancer patients.

Central-European journal of immunology

Biylgi O,Karagöz B,Türken O,Gültepe M,Özgün A,Tunçel T,Emirzeoğlu L,Çelik S,Müftüoğlu T,Gökhan Kandemir E

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Thu Jul 09 00:00:00 EDT 2015

Ex vivo analysis of resident hepatic pro-inflammatory CD1d-reactive T cells and hepatocyte surface CD1d expression in hepatitis C.

Journal of viral hepatitis

Yanagisawa K,Yue S,van der Vliet HJ,Wang R,Alatrakchi N,Golden-Mason L,Schuppan D,Koziel MJ,Rosen HR,Exley MA

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Thu Aug 01 00:00:00 EDT 2013

Upregulation of programmed death-1 on T cells and programmed death ligand-1 on monocytes in septic shock patients.

Critical care (London, England)

Zhang Y,Li J,Lou J,Zhou Y,Bo L,Zhu J,Zhu K,Wan X,Cai Z,Deng X

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Wed Dec 07 00:00:00 EST 2011

Sepsis-induced human lymphocyte apoptosis and cytokine production in "humanized" mice.

Journal of leukocyte biology

Unsinger J,McDonough JS,Shultz LD,Ferguson TA,Hotchkiss RS

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2009

B cells and monocytes from patients with active multiple sclerosis exhibit increased surface expression of both HERV-H Env and HERV-W Env, accompanied by increased seroreactivity.

Retrovirology

Brudek T,Christensen T,Aagaard L,Petersen T,Hansen HJ,Møller-Larsen A

Published figure using CD8a monoclonal antibody (Product # 47-0088-42) in Flow Cytometry

Mon Nov 16 00:00:00 EST 2009

Bioinformatics

Protein Aliases: CD8 antigen, alpha polypeptide (p32); CD8 antigen, beta polypeptide 1 (p37); CD8a; CD8alpha; CD8b; CD8beta; fCD8; Leu-2; leu-2a; Leu2 T-lymphocyte antigen; OKT8 T-cell antigen; T cell co-receptor; T lymphocyte surface glycoprotein beta chain; T-cell antigen Leu2; T-cell surface glycoprotein CD8 alpha chain; T-cell surface glycoprotein CD8 beta chain; T-lymphocyte differentiation antigen T8/Leu-2; T8 T-cell antigen

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Gene Aliases: CD8; CD8A; CD8B; CD8B1; LEU2; LY3; LYT3; MAL; p32; P37

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UniProt ID: (Human) P01732, (Human) P10966

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Entrez Gene ID: (Human) 925, (Human) 926

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Function(s)

protein binding coreceptor activity protein kinase binding MHC class I protein binding protein homodimerization activity defense/immunity protein immunoglobulin receptor superfamily

Process(es)

T cell mediated immunity immune response cell surface receptor signaling pathway transmembrane receptor protein tyrosine kinase signaling pathway antigen processing and presentation T cell activation cytotoxic T cell differentiation regulation of immune response positive regulation of calcium-mediated signaling defense response to virus adaptive immune response regulation of defense response to virus by virus

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8a Monoclonal Antibody (RPA-T8), APC-eFluor™ 780, eBioscience™

CD8a Antibody (47-0088-42) in Flow

Product Details

47-0088-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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