Invitrogen

CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), FITC, eBioscience™

8 Published Figures

18 References

View all (76) CD8b antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

FIGURE: 1 / 9

CD8b Antibody (11-0083-82) in Flow

Staining of BALB/c splenocytes with Anti-Mouse CD8a APC (Product # 17-0081-82) and 0.125 µg of Rat IgG2b kappa Isotype Control FITC (Product # 11-4031-82) (left) or 0.125 µg of Anti-Mouse CD8b FITC (right). Cells in the lymphocyte gate were used for analysis.

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Product Details

11-0083-82

Applications

Tested Dilution

Publications

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Flow Cytometry (Flow)

0.25 µg/test

View 16 publications 16 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), FITC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioH35-17.2 (H35-17.2)

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_657764

Product Specific Information

Description: The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.

Applications Reported: This eBioH35-17.2 (H35-17.2) antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eBioH35-17.2 (H35-17.2) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

IL-18BP mediates the balance between protective and pathological immune responses to Toxoplasma gondii.

Cell reports

Clark JT,Weizman OE,Aldridge DL,Shallberg LA,Eberhard J,Lanzar Z,Wasche D,Huck JD,Zhou T,Ring AM,Hunter CA

11-0083-82 was used in Flow cytometry/Cell sorting to investigate the role of interleukin-18 (IL-18) and its binding protein (IL-18BP) in controlling Toxoplasma gondii infection.

Tue Mar 28 00:00:00 EDT 2023

Neurokinin-1 Receptor Signaling Is Required for Efficient Ca²⁺ Flux in T-Cell-Receptor-Activated T Cells.

Cell reports

Morelli AE,Sumpter TL,Rojas-Canales DM,Bandyopadhyay M,Chen Z,Tkacheva O,Shufesky WJ,Wallace CT,Watkins SC,Berger A,Paige CJ,Falo LD,Larregina AT

11-0083 was used in Flow cytometry/Cell sorting to demonstrate an effect of the NK1R in T cells that is relevant for immunotherapies based on pro-inflammatory neuropeptides and its receptors.

Tue Mar 10 00:00:00 EDT 2020

Pharmacological reactivation of MYC-dependent apoptosis induces susceptibility to anti-PD-1 immunotherapy.

Nature communications

Haikala HM,Anttila JM,Marques E,Raatikainen T,Ilander M,Hakanen H,Ala-Hongisto H,Savelius M,Balboa D,Von Eyss B,Eskelinen V,Munne P,Nieminen AI,Otonkoski T,Schüler J,Laajala TD,Aittokallio T,Sihto H,Mattson J,Heikkilä P,Leidenius M,Joensuu H,Mustjoki S,Kovanen P,Eilers M,Leverson JD,Klefström J

11-0083-82 was used in Flow Cytometry to demonstrate that pharmacological reactivation of MYC-dependent apoptosis is a powerful antitumor strategy involving both tumor cell depletion and immunosurveillance.

Wed Feb 06 00:00:00 EST 2019

Adenoviral production of interleukin-2 at the tumor site removes the need for systemic postconditioning in adoptive cell therapy.

International journal of cancer

Santos JM,Havunen R,Siurala M,Cervera-Carrascon V,Tähtinen S,Sorsa S,Anttila M,Karell P,Kanerva A,Hemminki A

11-0083-82 was used in Flow Cytometry to study the efficacy and safety of intratumorally administered IL-2 coding adenoviruses in combination with lymphocyte therapy in Syrian hamsters bearing pancreatic tumours.

Sun Oct 01 00:00:00 EDT 2017

Peripheral administration of the soluble TNF inhibitor XPro1595 modifies brain immune cell profiles, decreases beta-amyloid plaque load, and rescues impaired long-term potentiation in 5xFAD mice.

Neurobiology of disease

MacPherson KP,Sompol P,Kannarkat GT,Chang J,Sniffen L,Wildner ME,Norris CM,Tansey MG

Published figure using CD8b monoclonal antibody (Product # 11-0083-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.

Nature communications

Zamarin D,Holmgaard RB,Ricca J,Plitt T,Palese P,Sharma P,Merghoub T,Wolchok JD,Allison JP

Published figure using CD8b monoclonal antibody (Product # 11-0083-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2017

Hydrogel dual delivered celecoxib and anti-PD-1 synergistically improve antitumor immunity.

Oncoimmunology

Li Y,Fang M,Zhang J,Wang J,Song Y,Shi J,Li W,Wu G,Ren J,Wang Z,Zou W,Wang L

11-0083 was used in Flow cytometry/Cell sorting to assess an alginate-hydrogel-mediated, combinatorial therapy of celecoxib and PD-1 mAb as a potential valuable regimen for treating human cancer.

Mon Feb 01 00:00:00 EST 2016

Tumor sialylation impedes T cell mediated anti-tumor responses while promoting tumor associated-regulatory T cells.

Oncotarget

Perdicchio M,Cornelissen LA,Streng-Ouwehand I,Engels S,Verstege MI,Boon L,Geerts D,van Kooyk Y,Unger WW

11-0083 was used in Flow cytometry/Cell sorting to study the influence of B16 melanoma hypersialylation on tumour growth.

Tue Feb 23 00:00:00 EST 2016

Favorable alteration of tumor microenvironment by immunomodulatory cytokines for efficient T-cell therapy in solid tumors.

PloS one

Tähtinen S,Kaikkonen S,Merisalo-Soikkeli M,Grönberg-Vähä-Koskela S,Kanerva A,Parviainen S,Vähä-Koskela M,Hemminki A

11-0083 was used in Flow cytometry/Cell sorting to alter the tumor microenvironment using immunomodulatory cytokines for efficient T-cell therapy in solid tumors.

Thu Apr 28 00:00:00 EDT 2016

Granulocyte/Macrophage Colony-stimulating Factor-dependent Dendritic Cells Restrain Lean Adipose Tissue Expansion.

The Journal of biological chemistry

Pamir N,Liu NC,Irwin A,Becker L,Peng Y,Ronsein GE,Bornfeldt KE,Duffield JS,Heinecke JW

11-0083 was used in Flow cytometry/Cell sorting to investigate dendritic cells and show cells that are dependent on GM-CSF restrain lean adipose tissue expansion.

Fri Jun 05 00:00:00 EDT 2015

Peptidylarginine deiminase 4 contributes to tumor necrosis factor α-induced inflammatory arthritis.

Arthritis & rheumatology (Hoboken, N.J.)

Shelef MA,Sokolove J,Lahey LJ,Wagner CA,Sackmann EK,Warner TF,Wang Y,Beebe DJ,Robinson WH,Huttenlocher A

11-0083 was used in Flow cytometry/Cell sorting to investigate whether TNFα and PAD4 may synergistically exacerbate autoantibody production and inflammatory arthritis, showing that PAD4 contributes to TNFα-induced inflammatory arthritis.

Sun Jun 01 00:00:00 EDT 2014

Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression.

Nature communications

Chen L,Gibbons DL,Goswami S,Cortez MA,Ahn YH,Byers LA,Zhang X,Yi X,Dwyer D,Lin W,Diao L,Wang J,Roybal J,Patel M,Ungewiss C,Peng D,Antonia S,Mediavilla-Varela M,Robertson G,Suraokar M,Welsh JW,Erez B,Wistuba II,Chen L,Peng D,Wang S,Ullrich SE,Heymach JV,Kurie JM,Qin FX

Published figure using CD8b monoclonal antibody (Product # 11-0083-82) in Flow Cytometry

Tue Oct 28 00:00:00 EDT 2014

Cutting edge: Heterosubtypic influenza infection antagonizes elicitation of immunological reactivity to hemagglutinin.

Journal of immunology (Baltimore, Md. : 1950)

Nayak JL,Alam S,Sant AJ

11-0083 was used in Flow cytometry/Cell sorting to evaluate the effect of competition between memory and naive lymphocytes on CD4 T cell and B cell response specificity using a murine model of sequential influenza infection.

Thu Aug 01 00:00:00 EDT 2013

Deletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency.

PloS one

Pioli PD,Dahlem TJ,Weis JJ,Weis JH

Published figure using CD8b monoclonal antibody (Product # 11-0083-82) in Flow Cytometry

Wed Feb 19 00:00:00 EST 2014

Lymphoma-like T cell infiltration in liver is associated with increased copy number of dominant negative form of TGFβ receptor II.

PloS one

Zhang W,Tsuda M,Yang GX,Tsuneyama K,He XS,Ansari AA,Ridgway WM,Coppel RL,Lian ZX,Leung PS,Gershwin ME

11-0083 was used in Flow cytometry/Cell sorting to further define the processes underlying the development of lymphoma-like T cell infiltration.

Tue Jun 25 00:00:00 EDT 2013

Expression of the chemokine binding protein M3 promotes marked changes in the accumulation of specific leukocytes subsets within the intestine.

Gastroenterology

Shang L,Thirunarayanan N,Viejo-Borbolla A,Martin AP,Bogunovic M,Marchesi F,Unkeless JC,Ho Y,Furtado GC,Alcami A,Merad M,Mayer L,Lira SA

Published figure using CD8b monoclonal antibody (Product # 11-0083-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2009

CD8alphaalpha T cells in lesions of Listeria monocytogenes-infected beta2m-deficient mice.

Microbial pathogenesis

Thoma-Uszynski S,Emoto M,Kaufmann SH

Fri Aug 01 00:00:00 EDT 1997

Depletion of T-cell subpopulations results in exacerbation of myocarditis and parasitism in experimental Chagas' disease.

Infection and immunity

Tarleton RL,Sun J,Zhang L,Postan M

Sun May 01 00:00:00 EDT 1994

Bioinformatics

Protein Aliases: CD8; CD8alpha; CD8b; CD8beta; Leu-2; Lymphocyte antigen 3; MAL; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-3

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Gene Aliases: Cd8b; Cd8b1; Ly-3; Ly-C; Lyt-3; Lyt3

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UniProt ID: (Mouse) P10300

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Entrez Gene ID: (Mouse) 12526

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Function(s)

protein binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

immune system process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), FITC, eBioscience™

CD8b Antibody (11-0083-82) in Flow

Product Details

11-0083-82

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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