Invitrogen

CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), eFluor™ 450, eBioscience™

8 Published Figures

11 References

View all (76) CD8b antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (8)

FIGURE: 1 / 9

CD8b Antibody (48-0083-82) in Flow

Staining of C57Bl/6 splenocytes with Anti-Mouse CD8a FITC (Product # 11-0081-82) and 0.125 µg of Rat IgG2b K Isotype Control eFluor® 450 (Product # 48-4031-82) (left) or 0.125 µg of Anti-Mouse CD8b eFluor® 450 (right). Total viable cells were used for analysis.

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Product Details

48-0083-82

Applications

Tested Dilution

Publications

Immunohistochemistry (IHC)

View 1 publication 1 publication

Immunohistochemistry (Frozen) (IHC (F))

View 2 publications 2 publications

Flow Cytometry (Flow)

0.25 µg/test

View 8 publications 8 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2b, kappa

Recommended Isotype Control

Rat IgG2b kappa Isotype Control (eB149/10H5), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBioH35-17.2 (H35-17.2)

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_11218504

Product Specific Information

Description: The eBioH35-17.2 monoclonal antibody reacts with the mouse CD8 beta molecule. The CD8 beta chain associates with the CD8 alpha chain to form the CD8 alpha/beta heterodimer expressed on the surface of a majority of thymocytes, and on peripheral cytotoxic alpha beta TCR T cells. CD8 binds to MHC class I and plays a role in T cell development and activation of mature T cells.

Applications Reported: This H35-17.2 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This H35-17.2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

The CD8B antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen, acting as a coreceptor, and the T-cell receptor on the T lymphocyte recognize antigens displayed by an antigen presenting cell (APC) in the context of class I MHC molecules. The functional coreceptor is either a homodimer composed of two alpha chains, or a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 beta chain isoforms. Multiple alternatively spliced transcript variants encoding distinct membrane associated or secreted isoforms have been described. A pseudogene, also located on chromosome 2, has been identified.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Heterogenous Populations of Tissue-Resident CD8⁺ T Cells Are Generated in Response to Infection and Malignancy.

Immunity

Milner JJ,Toma C,He Z,Kurd NS,Nguyen QP,McDonald B,Quezada L,Widjaja CE,Witherden DA,Crowl JT,Shaw LA,Yeo GW,Chang JT,Omilusik KD,Goldrath AW

48-0083 was used in Flow cytometry/Cell sorting to identify discrete lineages of intestinal antigen-specific CD8+ T cells, including a Blimp1hiId3lo tissue-resident effector cell population most prominent in the early phase of acute viral and bacterial infections and a molecularly distinct Blimp1loId3hi tissue-resident memory population that subsequently accumulated at later infection time points.

Tue May 19 00:00:00 EDT 2020

Cell-Type-Specific Responses to Interleukin-1 Control Microbial Invasion and Tumor-Elicited Inflammation in Colorectal Cancer.

Immunity

Dmitrieva-Posocco O,Dzutsev A,Posocco DF,Hou V,Yuan W,Thovarai V,Mufazalov IA,Gunzer M,Shilovskiy IP,Khaitov MR,Trinchieri G,Waisman A,Grivennikov SI

48-0083-82 was used in Flow Cytometry to examine the impact of IL-1 signaling, a key regulator of the IL-17 pathway, in different cell types within the CRC microenvironment.

Tue Jan 15 00:00:00 EST 2019

Peripheral administration of the soluble TNF inhibitor XPro1595 modifies brain immune cell profiles, decreases beta-amyloid plaque load, and rescues impaired long-term potentiation in 5xFAD mice.

Neurobiology of disease

MacPherson KP,Sompol P,Kannarkat GT,Chang J,Sniffen L,Wildner ME,Norris CM,Tansey MG

Published figure using CD8b monoclonal antibody (Product # 48-0083-82) in Flow Cytometry

Thu Jun 01 00:00:00 EDT 2017

Intratumoral modulation of the inducible co-stimulator ICOS by recombinant oncolytic virus promotes systemic anti-tumour immunity.

Nature communications

Zamarin D,Holmgaard RB,Ricca J,Plitt T,Palese P,Sharma P,Merghoub T,Wolchok JD,Allison JP

Published figure using CD8b monoclonal antibody (Product # 48-0083-82) in Flow Cytometry

Mon Feb 13 00:00:00 EST 2017

Multimodal analysis of ocular inflammation using the endotoxin-induced uveitis mouse model.

Disease models & mechanisms

Chu CJ,Gardner PJ,Copland DA,Liyanage SE,Gonzalez-Cordero A,Kleine Holthaus SM,Luhmann UF,Smith AJ,Ali RR,Dick AD

48-0083 was used in Immunohistochemistry to develop a comprehensive and efficient technique for assessing EIU severity that utilises ocular dissection, dissociation, and matched multimodal tissue analysis.

Fri Apr 01 00:00:00 EDT 2016

Inflammasome-independent role of AIM2 in suppressing colon tumorigenesis via DNA-PK and Akt.

Nature medicine

Wilson JE,Petrucelli AS,Chen L,Koblansky AA,Truax AD,Oyama Y,Rogers AB,Brickey WJ,Wang Y,Schneider M,Mühlbauer M,Chou WC,Barker BR,Jobin C,Allbritton NL,Ramsden DA,Davis BK,Ting JP

48-0083 was used in Flow cytometry/Cell sorting to show AIM2 regulates colon tumorigenesis by reducing activation of both DNA-PK and Akt, suggesting that Akt inhibitors could be used in treatment of certain cancers.

Sat Aug 01 00:00:00 EDT 2015

Metastasis is regulated via microRNA-200/ZEB1 axis control of tumour cell PD-L1 expression and intratumoral immunosuppression.

Nature communications

Chen L,Gibbons DL,Goswami S,Cortez MA,Ahn YH,Byers LA,Zhang X,Yi X,Dwyer D,Lin W,Diao L,Wang J,Roybal J,Patel M,Ungewiss C,Peng D,Antonia S,Mediavilla-Varela M,Robertson G,Suraokar M,Welsh JW,Erez B,Wistuba II,Chen L,Peng D,Wang S,Ullrich SE,Heymach JV,Kurie JM,Qin FX

Published figure using CD8b monoclonal antibody (Product # 48-0083-82) in Flow Cytometry

Tue Oct 28 00:00:00 EDT 2014

Deletion of Snai2 and Snai3 results in impaired physical development compounded by lymphocyte deficiency.

PloS one

Pioli PD,Dahlem TJ,Weis JJ,Weis JH

Published figure using CD8b monoclonal antibody (Product # 48-0083-82) in Flow Cytometry

Wed Feb 19 00:00:00 EST 2014

Expression of the chemokine binding protein M3 promotes marked changes in the accumulation of specific leukocytes subsets within the intestine.

Gastroenterology

Shang L,Thirunarayanan N,Viejo-Borbolla A,Martin AP,Bogunovic M,Marchesi F,Unkeless JC,Ho Y,Furtado GC,Alcami A,Merad M,Mayer L,Lira SA

Published figure using CD8b monoclonal antibody (Product # 48-0083-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2009

CD8alphaalpha T cells in lesions of Listeria monocytogenes-infected beta2m-deficient mice.

Microbial pathogenesis

Thoma-Uszynski S,Emoto M,Kaufmann SH

Fri Aug 01 00:00:00 EDT 1997

Depletion of T-cell subpopulations results in exacerbation of myocarditis and parasitism in experimental Chagas' disease.

Infection and immunity

Tarleton RL,Sun J,Zhang L,Postan M

Sun May 01 00:00:00 EDT 1994

Bioinformatics

Protein Aliases: CD8; CD8alpha; CD8b; CD8beta; Leu-2; Lymphocyte antigen 3; MAL; T-cell membrane glycoprotein Ly-3; T-cell surface glycoprotein CD8 beta chain; T-cell surface glycoprotein Lyt-3

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Gene Aliases: Cd8b; Cd8b1; Ly-3; Ly-C; Lyt-3; Lyt3

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UniProt ID: (Mouse) P10300

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Entrez Gene ID: (Mouse) 12526

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Function(s)

protein binding defense/immunity protein immunoglobulin receptor superfamily

Process(es)

immune system process

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD8b Monoclonal Antibody (eBioH35-17.2 (H35-17.2)), eFluor™ 450, eBioscience™

CD8b Antibody (48-0083-82) in Flow

Product Details

48-0083-82

Immunohistochemistry (IHC)

Immunohistochemistry (Frozen) (IHC (F))

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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