Invitrogen

CD95 (APO-1/Fas) Monoclonal Antibody (15A7), APC, eBioscience™

CD95 (APO-1/Fas) Antibody in Flow Cytometry (Flow)

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CD95 (APO-1/Fas) Antibody (17-0951-82) in Flow

Staining of BALB/c thymocytes with 1 µg of Mouse IgG1 K Isotype Control APC (Product # 17-4714-81) (blue histogram) or 1 µg of Anti-Mouse CD95 (APO-1/Fas) APC (purple histogram). Total viable cells were used for analysis.

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Product Details

17-0951-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

1 µg/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Mouse

Published species

Human, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), APC, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

15A7

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10717405

Product Specific Information

Description: The 15A7 monoclonal antibody reacts with mouse CD95 (Fas, Apo-1), a 40-50 kDa member of the TNFR superfamily. CD95 is expressed by a broad range of hematopoietic and non-hematopoietic cells including monocytes, neutrophils, activated lymphocytes and fibroblasts. Interaction of CD95 on mature lymphocytes with its ligand (FasL) induces apoptosis and is thought to be important in peripheral tolerance. 15A7 reacts with mouse CD95 in BALB/c strain and a bit more weakly in C57Bl/6 strain. 15A7 does not induce apoptosis of Fas-expressing cells.

Applications Reported: This 15A7 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This 15A7 antibody has been tested by flow cytometric analysis of mouse thymocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FAS (Fas, CD95, APO-1) is a 46 kDa transmembrane glycoprotein that functions as a cell death receptor of the TNFR (tumor necrosis factor receptor) superfamily. FAS also exists in a soluble form that weight approximately 26kDa. Stimulation of FAS results in aggregation of its intracellular FAS-associated death domains (FADD), formation of the death-inducing signaling complex (DISC) and activation of caspases. The auto-proteolytic processing of the caspases in the complex trigger a downstream cascade that leads to apoptosis. FAS has been shown to activate NF-kappaB, MAPK3/ERK1, MAPK8/JNK, and is involved in transducing the proliferating signals in normal diploid fibroblast and T cells. At least eight alternatively spliced transcript variants encoding seven distinct isoforms have been described. The isoforms lacking the transmembrane domain may negatively regulate the apoptosis mediated by the full-length isoform. The Fas/Fas ligand system has been shown to play a role in a number of human diseases such as AIDS, hepatitis and cancer. It is also assumed that induction of apoptosis through FAS ligand pathway is predominantly involved in anti-viral immune responses.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

The histone H2B ubiquitination regulator Wac is essential for plasma cell differentiation.

FEBS letters

Li Y,Ruan GX,Chen W,Huang H,Zhang R,Wang J,Ouyang Y,Zhu Z,Meng L,Wang R,Huo J,Xu S,Ou X

17-0951-82 was used in Flow Cytometry to demonstrate an essential role of Wac-mediated ubH2B in PC differentiation and shed light on the epigenetic mechanisms underlying this process.

Sat Jul 01 00:00:00 EDT 2023

CD4⁺ CTLs Act as a Key Effector Population for Allograft Rejection of MSCs in a Donor MHC-II Dependent Manner in Injured Liver.

Aging and disease

Shen S,Li Y,Jin M,Fan D,Pan R,Lin A,Chen Y,Xiang L,Zhao RC,Shao J

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 17-0951-82) in Flow Cytometry

Thu Dec 01 00:00:00 EST 2022

Elimination of receptor binding by influenza hemagglutinin improves vaccine-induced immunity.

NPJ vaccines

Hendin HE,Lavoie PO,Gravett JM,Pillet S,Saxena P,Landry N,D'Aoust MA,Ward BJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 17-0951-82) in Flow Cytometry

Mon Apr 11 00:00:00 EDT 2022

Characterization of the spectrum of trivalent VAV1-mutation-driven tumours using a gene-edited mouse model.

Molecular oncology

Robles-Valero J,Fernández-Nevado L,Cuadrado M,Lorenzo-Martín LF,Fernández-Pisonero I,Abad A,Redín E,Montuenga L,Martín-Zanca D,Bigas A,Mallo M,Dosil M,Bustelo XR

17-0951-82 was used in Flow cytometry/Cell sorting to indicate that VAV1 mutations play critical, although highly cell-type-specific, roles in tumourigenesis.

Sat Oct 01 00:00:00 EDT 2022

The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity.

Cellular & molecular immunology

Huang X,Hao S,Liu J,Huang Y,Liu M,Xiao C,Wang Y,Pei S,Yu T,Xu J,Wang H,Dai D,Su X,Xiao Y

17-0951-82 was used in Flow Cytometry to elucidate the molecular mechanisms regulating T follicular helper cells.

Thu Apr 01 00:00:00 EDT 2021

The ubiquitin ligase Peli1 inhibits ICOS and thereby Tfh-mediated immunity.

Cellular & molecular immunology

Huang X,Hao S,Liu J,Huang Y,Liu M,Xiao C,Wang Y,Pei S,Yu T,Xu J,Wang H,Dai D,Su X,Xiao Y

17-0951-82 was used in Flow Cytometry to elucidate the molecular mechanisms regulating T follicular helper cells.

Thu Apr 01 00:00:00 EDT 2021

Berberine modulates hyper-inflammation in mouse macrophages stimulated with polyinosinic-polycytidylic acid via calcium-CHOP/STAT pathway.

Scientific reports

Kim HJ,Kim YJ,Park W

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 17-0951-82) in Flow Cytometry

Fri May 28 00:00:00 EDT 2021

CCL9/CCR1 induces myeloid‑derived suppressor cell recruitment to the spleen in a murine H22 orthotopic hepatoma model.

Oncology reports

Li B,Zhang S,Huang N,Chen H,Wang P,Yang J,Li Z

17-0951-82 was used in Flow Cytometry to indicate that CCL9 secreted by splenic macrophages induces a C-C motif chemokine receptor 1-dependent accumulation of myeloid-derived suppressor cells in the spleen in a murine H22 hepatoma model.

Tue Jan 01 00:00:00 EST 2019

Initiation of Antiviral B Cell Immunity Relies on Innate Signals from Spatially Positioned NKT Cells.

Cell

Gaya M,Barral P,Burbage M,Aggarwal S,Montaner B,Warren Navia A,Aid M,Tsui C,Maldonado P,Nair U,Ghneim K,Fallon PG,Sekaly RP,Barouch DH,Shalek AK,Bruckbauer A,Strid J,Batista FD

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 17-0951-82) in Flow Cytometry

Thu Jan 25 00:00:00 EST 2018

Transforming Growth Factor-β and Interleukin-10 Synergistically Regulate Humoral Immunity via Modulating Metabolic Signals.

Frontiers in immunology

Komai T,Inoue M,Okamura T,Morita K,Iwasaki Y,Sumitomo S,Shoda H,Yamamoto K,Fujio K

17-0951 was used in Flow cytometry/Cell sorting to study the requirement of a combination of TGF-Β and IL-10 to suppress B cell activation induced by toll-like receptor stimulation.

Wed Nov 20 00:00:00 EST 2019

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 17-0951-82) in Flow Cytometry

Mon Mar 19 00:00:00 EDT 2018

γδ T cells control humoral immune response by inducing T follicular helper cell differentiation.

Nature communications

Rezende RM,Lanser AJ,Rubino S,Kuhn C,Skillin N,Moreira TG,Liu S,Gabriely G,David BA,Menezes GB,Weiner HL

17-0951 was used in Flow cytometry/Cell sorting to demonstrate that complete Freund's adjuvant immunisation induces a subpopulation of CXCR5-expressing γδ T cells in the draining lymph nodes.

Wed Aug 08 00:00:00 EDT 2018

Vaccine-induced Th17 cells are established as resident memory cells in the lung and promote local IgA responses.

Mucosal immunology

Christensen D,Mortensen R,Rosenkrands I,Dietrich J,Andersen P

17-0951 was used in Flow cytometry/Cell sorting to demonstrate that subcutaneous priming with a recombinant antigen in a T helper (Th)17-inducing adjuvant followed by airway boosting promotes high and sustained levels of IgA in the lungs.

Sun Jan 01 00:00:00 EST 2017

Armored CAR T cells enhance antitumor efficacy and overcome the tumor microenvironment.

Scientific reports

Yeku OO,Purdon TJ,Koneru M,Spriggs D,Brentjens RJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 17-0951-82) in Flow Cytometry

Tue Sep 05 00:00:00 EDT 2017

YY1 plays an essential role at all stages of B-cell differentiation.

Proceedings of the National Academy of Sciences of the United States of America

Kleiman E,Jia H,Loguercio S,Su AI,Feeney AJ

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 17-0951-82) in Flow Cytometry

Tue Jul 05 00:00:00 EDT 2016

YY1 Regulates the Germinal Center Reaction by Inhibiting Apoptosis.

Journal of immunology (Baltimore, Md. : 1950)

Trabucco SE,Gerstein RM,Zhang H

17-0951 was used in Flow cytometry/Cell sorting to understand the role of transcription factor YY1 in the regulation of the germinal centre reaction in mice.

Thu Sep 01 00:00:00 EDT 2016

Helios Controls a Limited Subset of Regulatory T Cell Functions.

Journal of immunology (Baltimore, Md. : 1950)

Sebastian M,Lopez-Ocasio M,Metidji A,Rieder SA,Shevach EM,Thornton AM

17-0951 was used in Flow cytometry/Cell sorting to determine the beneficial effects of the transcription factor, Helios, for Treg maintenance, differentiation, and survival.

Fri Jan 01 00:00:00 EST 2016

The histone lysine methyltransferase KMT2D sustains a gene expression program that represses B cell lymphoma development.

Nature medicine

Ortega-Molina A,Boss IW,Canela A,Pan H,Jiang Y,Zhao C,Jiang M,Hu D,Agirre X,Niesvizky I,Lee JE,Chen HT,Ennishi D,Scott DW,Mottok A,Hother C,Liu S,Cao XJ,Tam W,Shaknovich R,Garcia BA,Gascoyne RD,Ge K,Shilatifard A,Elemento O,Nussenzweig A,Melnick AM,Wendel HG

17-0951 was used in Flow cytometry/Cell sorting to show that KMT2D mutations may promote malignant outgrowth by perturbing the expression of genes that control B cell-activating pathways.

Thu Oct 01 00:00:00 EDT 2015

Suppression of Th2 and Tfh immune reactions by Nr4a receptors in mature T reg cells.

The Journal of experimental medicine

Sekiya T,Kondo T,Shichita T,Morita R,Ichinose H,Yoshimura A

17-0951 was used in Flow cytometry/Cell sorting to demonstrate a crucial role for Nr4a factors in controlling a genetic program that is indispensable for Treg cell maintenance and function.

Mon Sep 21 00:00:00 EDT 2015

Substituting threonine 187 with alanine in p27Kip1 prevents pituitary tumorigenesis by two-hit loss of Rb1 and enhances humoral immunity in old age.

The Journal of biological chemistry

Zhao H,Bauzon F,Bi E,Yu JJ,Fu H,Lu Z,Cui J,Jeon H,Zang X,Ye BH,Zhu L

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 17-0951-82) in Flow Cytometry

Fri Feb 27 00:00:00 EST 2015

Reactive oxygen species deficiency induces autoimmunity with type 1 interferon signature.

Antioxidants & redox signaling

Kelkka T,Kienhöfer D,Hoffmann M,Linja M,Wing K,Sareila O,Hultqvist M,Laajala E,Chen Z,Vasconcelos J,Neves E,Guedes M,Marques L,Krönke G,Helminen M,Kainulainen L,Olofsson P,Jalkanen S,Lahesmaa R,Souto-Carneiro MM,Holmdahl R

17-0951 was used in Flow cytometry/Cell sorting to explore the connection between reactive oxygen species deficiencies and increased susceptibility to autoimmunity.

Mon Dec 01 00:00:00 EST 2014

Up-regulation of Fas (CD95) expression in tumour cells in vivo.

Immunology

Peshes-Yaloz N,Rosen D,Sondel PM,Krammer PH,Berke G

Published figure using CD95 (APO-1/Fas) monoclonal antibody (Product # 17-0951-82) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2007

Bioinformatics

Protein Aliases: Apo-1 antigen; Apo-1 Fas; Apo1 antigen; Apoptosis-mediating surface antigen FAS; CD95; Fas (TNF receptor superfamily member); Fas antigen; FASLG receptor; lymphoproliferation; OTTHUMP00000020045; OTTHUMP00000020046; OTTHUMP00000020051; OTTHUMP00000059646; Tumor necrosis factor receptor superfamily member 6

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Gene Aliases: AI196731; APO1; APT1; CD95; Fas; lpr; TNFR6; Tnfrsf6

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UniProt ID: (Mouse) P25446

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Entrez Gene ID: (Mouse) 14102

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Function(s)

protease binding transmembrane signaling receptor activity tumor necrosis factor-activated receptor activity protein binding kinase binding protein complex binding identical protein binding transmembrane signal receptor

Process(es)

immunoglobulin production renal system process apoptotic process activation-induced cell death of T cells inflammatory cell apoptotic process obsolete transformed cell apoptotic process immune response signal transduction extrinsic apoptotic signaling pathway via death domain receptors response to toxic substance gene expression regulation of gene expression positive regulation of cell death B cell mediated immunity dendrite regeneration positive regulation of protein homooligomerization tumor necrosis factor-mediated signaling pathway regulation of cell proliferation regulation of apoptotic process T cell homeostasis positive regulation of apoptotic process negative regulation of apoptotic process positive regulation of cysteine-type endopeptidase activity involved in apoptotic process negative thymic T cell selection regulation of lymphocyte differentiation regulation of myeloid cell differentiation spleen development negative regulation of B cell activation protein homooligomerization response to glucocorticoid neuron apoptotic process cellular response to lithium ion cellular response to hyperoxia positive regulation of release of cytochrome c from mitochondria motor neuron apoptotic process apoptotic signaling pathway extrinsic apoptotic signaling pathway extrinsic apoptotic signaling pathway in absence of ligand hepatocyte apoptotic process necroptotic signaling pathway positive regulation of extrinsic apoptotic signaling pathway in absence of ligand

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CD95 (APO-1/Fas) Monoclonal Antibody (15A7), APC, eBioscience™

CD95 (APO-1/Fas) Antibody (17-0951-82) in Flow

Product Details

17-0951-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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