Invitrogen

EOMES Monoclonal Antibody (WD1928), PE-eFluor™ 610, eBioscience™

FIGURE: 1 / 17

EOMES Antibody (61-4877-42) in Flow

Normal human peripheral blood cells were surface stained with Anti-Human CD56 (NCAM) PerCP-eFluor® 710 (Product # 46-0567-42), then intracellularly stained with Mouse IgG1 K Isotype Control PE-eFluor® 610 (Product # 61-4714-82) (left) or Anti-Human EOMES PE-eFluor® 610 (right) using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Cells in the lymphocyte gate were used for analysis.

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Product Details

61-4877-42

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 25 publications 25 publications

Product Specifications

Species Reactivity

Human, Pig

Published species

Human, Mammal, Mouse

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-eFluor™ 610, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

WD1928

Conjugate

PE-eFluor™ 610 PE-eFluor™ 610 PE-eFluor™ 610

Excitation/Emission Max

565/606 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2574616

Product Specific Information

Description: This WD1928 antibody recognizes Eomesodermin (Eomes), also known as T-box brain 2 (TBR2). Eomes is a T-box transcription factor that is highly homologous to T-bet, which is essential during trophoblast development and gastrulation in most vertebrates. In the immune system, Eomes controls the differentiation of effector and memory CD8+ T cells, as well as natural killer (NK) cells. Expression of Eomes in these cells correlates with high expression of CD122, the common beta-chain of the IL-2R and IL-15R.

Applications Reported: This WD1928 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This WD1928 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Refer to Best Protocols for Staining Protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

TBR2 is a member of a conserved protein family that shares a common DNA-binding domain, the T-box. T-box genes encode transcription factors involved in the regulation of developmental processes. A similiar protein disrupted in mice is shown to be essential during trophoblast development and gastrulation. TBR2 plays a role in brain development and is required for the specification and the proliferation of the intermediate progenitor cells and their progeny in the cerebral cortex.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

A single-cell map of dynamic chromatin landscapes of immune cells in renal cell carcinoma.

Nature cancer

Kourtis N,Wang Q,Wang B,Oswald E,Adler C,Cherravuru S,Malahias E,Zhang L,Golubov J,Wei Q,Lemus S,Ni M,Ding Y,Wei Y,Atwal GS,Thurston G,Macdonald LE,Murphy AJ,Dhanik A,Sleeman MA,Tykodi SS,Skokos D

Published figure using EOMES monoclonal antibody (Product # 61-4877-42) in Flow Cytometry

Fri Jul 01 00:00:00 EDT 2022

Transcriptional profiling of human Vδ1 T cells reveals a pathogen-driven adaptive differentiation program.

Cell reports

McMurray JL,von Borstel A,Taher TE,Syrimi E,Taylor GS,Sharif M,Rossjohn J,Remmerswaal EBM,Bemelman FJ,Vieira Braga FA,Chen X,Teichmann SA,Mohammed F,Berry AA,Lyke KE,Williamson KC,Stubbington MJT,Davey MS,Willcox CR,Willcox BE

Published figure using EOMES monoclonal antibody (Product # 61-4877-42) in Flow Cytometry

Tue May 24 00:00:00 EDT 2022

Transcriptional Pattern Analysis of Virus-Specific CD8+ T Cells in Hepatitis C Infection: Increased Expression of TOX and Eomesodermin During and After Persistent Antigen Recognition.

Frontiers in immunology

Wildner NH,Walker A,Brauneck F,Ditt V,Peine S,Huber S,Haag F,Beisel C,Timm J,Schulze Zur Wiesch J

61-4877-42 was used in Flow cytometry/Cell sorting to define on-target T cells as T cells against epitopes without escape mutations and off-target T cells as those against a historical" antigen mutated in the autologous sequence."

Fri Jun 24 00:00:00 EDT 2022

Comparison of dynamic changes in the peripheral CD8⁺ T cells function and differentiation in ESCC patients treated with radiotherapy combined with anti-PD-1 antibody or concurrent chemoradiotherapy.

Frontiers in immunology

Wei H,Li Y,Guo Z,Ma X,Li Y,Wei X,Han D,Zhang T,Chen X,Yan C,Zhou J,Pang Q,Wang P,Zhang W

61-4877-42 was used in Flow cytometry/Cell sorting to identify the dynamic changes of systematic immune status of patients undergoing treatment. The two treatments had similar activation effects on peripheral CD8+ T cells with different PD-1 properties but had different effects on their differentiation status.

Thu Dec 15 00:00:00 EST 2022

Culture, expansion, and flow-cytometry-based functional analysis of pteropid bat MR1-restricted unconventional T cells.

STAR protocols

Sia WR,Hey YY,Foo R,Wang LF,Leeansyah E

61-4877 was used in Flow cytometry/Cell sorting to examine pteropid MR1T cell functionality, including proliferative capacity, cytotoxicity, and cytokine production.

Fri Jun 18 00:00:00 EDT 2021

Culture, expansion, and flow-cytometry-based functional analysis of pteropid bat MR1-restricted unconventional T cells.

STAR protocols

Sia WR,Hey YY,Foo R,Wang LF,Leeansyah E

61-4877 was used in Flow cytometry/Cell sorting to examine pteropid MR1T cell functionality, including proliferative capacity, cytotoxicity, and cytokine production.

Fri Jun 18 00:00:00 EDT 2021

Norovirus-Specific CD8⁺ T Cell Responses in Human Blood and Tissues.

Cellular and molecular gastroenterology and hepatology

Pattekar A,Mayer LS,Lau CW,Liu C,Palko O,Bewtra M,Consortium H,Lindesmith LC,Brewer-Jensen PD,Baric RS,Betts MR,Naji A,Wherry EJ,Tomov VT

Published figure using EOMES monoclonal antibody (Product # 61-4877-42) in Flow Cytometry

Tue Mar 08 00:00:00 EST 2022

Homeostatic Function and Inflammatory Activation of Ileal CD8⁺ Tissue-Resident T Cells Is Dependent on Mucosal Location.

Cellular and molecular gastroenterology and hepatology

Lutter L,Roosenboom B,Brand EC,Ter Linde JJ,Oldenburg B,van Lochem EG,Horjus Talabur Horje CS,van Wijk F

Published figure using EOMES monoclonal antibody (Product # 61-4877-42) in Flow Cytometry

Mon Mar 28 00:00:00 EDT 2022

Cell-type-specific 3D epigenomes in the developing human cortex.

Nature

Song M,Pebworth MP,Yang X,Abnousi A,Fan C,Wen J,Rosen JD,Choudhary MNK,Cui X,Jones IR,Bergenholtz S,Eze UC,Juric I,Li B,Maliskova L,Lee J,Liu W,Pollen AA,Li Y,Wang T,Hu M,Kriegstein AR,Shen Y

Published figure using EOMES monoclonal antibody (Product # 61-4877-42) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2020

Developmental Relationships of Four Exhausted CD8⁺ T Cell Subsets Reveals Underlying Transcriptional and Epigenetic Landscape Control Mechanisms.

Immunity

Beltra JC,Manne S,Abdel-Hakeem MS,Kurachi M,Giles JR,Chen Z,Casella V,Ngiow SF,Khan O,Huang YJ,Yan P,Nzingha K,Xu W,Amaravadi RK,Xu X,Karakousis GC,Mitchell TC,Schuchter LM,Huang AC,Wherry EJ

61-4877-42 was used in Flow cytometry/Cell sorting to reveal a four-stage developmental hierarchy for Tex cells and define the molecular, transcriptional, and epigenetic mechanisms that could provide opportunities to improve cancer immunotherapy.

Tue May 19 00:00:00 EDT 2020

Complement opsonization of HIV affects primary infection of human colorectal mucosa and subsequent activation of T cells.

eLife

Bhattacharya P,Ellegård R,Khalid M,Svanberg C,Govender M,Keita ÅV,Söderholm JD,Myrelid P,Shankar EM,Nyström S,Larsson M

Published figure using EOMES monoclonal antibody (Product # 61-4877-42) in Flow Cytometry

Wed Sep 02 00:00:00 EDT 2020

The CD38/NAD/SIRTUIN1/EZH2 Axis Mitigates Cytotoxic CD8 T Cell Function and Identifies Patients with SLE Prone to Infections.

Cell reports

Katsuyama E,Suarez-Fueyo A,Bradley SJ,Mizui M,Marin AV,Mulki L,Krishfield S,Malavasi F,Yoon J,Sui SJH,Kyttaris VC,Tsokos GC

Published figure using EOMES monoclonal antibody (Product # 61-4877-42) in Flow Cytometry

Tue Jan 07 00:00:00 EST 2020

CD19-CAR-T Cells Bearing a KIR/PD-1-Based Inhibitory CAR Eradicate CD19⁺HLA-C1^- Malignant B Cells While Sparing CD19⁺HLA-C1⁺ Healthy B Cells.

Cancers

Tao L,Farooq MA,Gao Y,Zhang L,Niu C,Ajmal I,Zhou Y,He C,Zhao G,Yao J,Liu M,Jiang W

Published figure using EOMES monoclonal antibody (Product # 61-4877-42) in Flow Cytometry

Sun Sep 13 00:00:00 EDT 2020

C reactive protein impairs adaptive immunity in immune cells of patients with melanoma.

Journal for immunotherapy of cancer

Yoshida T,Ichikawa J,Giuroiu I,Laino AS,Hao Y,Krogsgaard M,Vassallo M,Woods DM,Stephen Hodi F,Weber J

Published figure using EOMES monoclonal antibody (Product # 61-4877-42) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

Umbilical cord blood-derived ILC1-like cells constitute a novel precursor for mature KIR⁺NKG2A^- NK cells.

eLife

Bennstein SB,Weinhold S,Manser AR,Scherenschlich N,Noll A,Raba K,Kögler G,Walter L,Uhrberg M

61-4877 was used in Flow cytometry/Cell sorting to support a model in which circulating fetal ILC1-like NKPs travel to secondary lymphoid tissues to initiate the formation of diversified NK cell repertoires after birth.

Mon Jul 13 00:00:00 EDT 2020

High CD26 and Low CD94 Expression Identifies an IL-23 Responsive Vδ2⁺ T Cell Subset with a MAIT Cell-like Transcriptional Profile.

Cell reports

Wragg KM,Tan HX,Kristensen AB,Nguyen-Robertson CV,Kelleher AD,Parsons MS,Wheatley AK,Berzins SP,Pellicci DG,Kent SJ,Juno JA

61-4877-42 was used in Flow Cytometry to demonstrate if CD26 and CD94 define transcriptionally, phenotypically, and functionally distinct Vδ2+ T cell subsets.

Tue Jun 16 00:00:00 EDT 2020

The TLR9 ligand CpG ODN 2006 is a poor adjuvant for the induction of de novo CD8⁺ T-cell responses in vitro.

Scientific reports

Papagno L,Kuse N,Lissina A,Gostick E,Price DA,Appay V,Nicoli F

Published figure using EOMES monoclonal antibody (Product # 61-4877-42) in Flow Cytometry

Wed Jul 15 00:00:00 EDT 2020

The TLR9 ligand CpG ODN 2006 is a poor adjuvant for the induction of de novo CD8⁺ T-cell responses in vitro.

Scientific reports

Papagno L,Kuse N,Lissina A,Gostick E,Price DA,Appay V,Nicoli F

Published figure using EOMES monoclonal antibody (Product # 61-4877-42) in Flow Cytometry

Wed Jul 15 00:00:00 EDT 2020

Unique transcriptional and protein-expression signature in human lung tissue-resident NK cells.

Nature communications

Marquardt N,Kekäläinen E,Chen P,Lourda M,Wilson JN,Scharenberg M,Bergman P,Al-Ameri M,Hård J,Mold JE,Ljunggren HG,Michaëlsson J

Published figure using EOMES monoclonal antibody (Product # 61-4877-42) in Flow Cytometry

Mon Aug 26 00:00:00 EDT 2019

Tissue-resident Eomes⁺ NK cells are the major innate lymphoid cell population in human infant intestine.

Nature communications

Sagebiel AF,Steinert F,Lunemann S,Körner C,Schreurs RRCE,Altfeld M,Perez D,Reinshagen K,Bunders MJ

Published figure using EOMES monoclonal antibody (Product # 61-4877-42) in Flow Cytometry

Thu Feb 28 00:00:00 EST 2019

The Obese Liver Environment Mediates Conversion of NK Cells to a Less Cytotoxic ILC1-Like Phenotype.

Frontiers in immunology

Cuff AO,Sillito F,Dertschnig S,Hall A,Luong TV,Chakraverty R,Male V

Published figure using EOMES monoclonal antibody (Product # 61-4877-42) in Flow Cytometry

Wed Oct 21 00:00:00 EDT 2020

Detection of HIV-1-specific gastrointestinal tissue resident CD8⁺ T-cells in chronic infection.

Mucosal immunology

Kiniry BE,Li S,Ganesh A,Hunt PW,Somsouk M,Skinner PJ,Deeks SG,Shacklett BL

61-4877 was used in Flow cytometry/Cell sorting to suggest a large fraction of CD8+ T-cells housed in the human rectosigmoid mucosa are tissue-resident and that TRM contribute to the anti-HIV-1 immune response.

Tue May 01 00:00:00 EDT 2018

PD1 is expressed on exhausted T cells as well as virus specific memory CD8+ T cells in the bone marrow of myeloma patients.

Oncotarget

Sponaas AM,Yang R,Rustad EH,Standal T,Thoresen AS,Dao Vo C,Waage A,Slørdahl TS,Børset M,Sundan A

61-4877 was used in Flow cytometry to study the expression of PD-1 on exhausted T cells as well as virus specific memory CD8+ T cells in the bone marrow of myeloma patients.

Fri Aug 10 00:00:00 EDT 2018

Predominance of weakly cytotoxic, T-bet^LowEomes^Neg CD8⁺ T-cells in human gastrointestinal mucosa: implications for HIV infection.

Mucosal immunology

Kiniry BE,Ganesh A,Critchfield JW,Hunt PW,Hecht FM,Somsouk M,Deeks SG,Shacklett BL

61-4877 was used in Flow cytometry/Cell sorting to report on the gastrointestinal mucosa site during HIV infection and suggest that rectal CD8+ T-cells are primarily non-cytotoxic, and phenotypically shaped by the tissue microenvironment.

Sat Jul 01 00:00:00 EDT 2017

Eomesodermin and T-bet mark developmentally distinct human natural killer cells.

JCI insight

Collins A,Rothman N,Liu K,Reiner SL

61-4877 was used in Flow cytometry/Cell sorting to demonstrate the expression pattern of signature NK cell transcription factors during developmental maturation.

Thu Mar 09 00:00:00 EST 2017

Eomeshi NK Cells in Human Liver Are Long-Lived and Do Not Recirculate but Can Be Replenished from the Circulation.

Journal of immunology (Baltimore, Md. : 1950)

Cuff AO,Robertson FP,Stegmann KA,Pallett LJ,Maini MK,Davidson BR,Male V

61-4877 was used in Flow cytometry/Cell sorting to study the molecular signal that characterises an Eomeshi population of natural killer cells in the human liver that is not present in the blood.

Thu Dec 01 00:00:00 EST 2016

Simultaneous, Single-Cell Measurement of Messenger RNA, Cell Surface Proteins, and Intracellular Proteins.

Current protocols in cytometry

Soh KT,Tario JD,Colligan S,Maguire O,Pan D,Minderman H,Wallace PK

61-4877 was used in Flow cytometry/Cell sorting to develop a protocol for the simultaneous, single-cell measurement of mRNA, cell surface proteins, and intracellular proteins.

Wed Jan 06 00:00:00 EST 2016

Bioinformatics

Protein Aliases: Eomesodermin homolog; T-box brain protein 2; T-brain-2

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Gene Aliases: EOMES; TBR2

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UniProt ID: (Human) O95936

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Entrez Gene ID: (Human) 8320, (Pig) 100622125

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Function(s)

RNA polymerase II regulatory region sequence-specific DNA binding RNA polymerase II activating transcription factor binding transcriptional repressor activity, RNA polymerase II transcription factor binding DNA binding chromatin binding transcription factor activity, sequence-specific DNA binding sequence-specific DNA binding Rel homology transcription factor

Process(es)

negative regulation of transcription from RNA polymerase II promoter endoderm formation mesoderm formation endodermal cell fate specification trophectodermal cell differentiation adaptive immune response CD8-positive, alpha-beta T cell differentiation involved in immune response transcription, DNA-templated brain development cardioblast differentiation stem cell population maintenance olfactory bulb development cerebral cortex regionalization cerebral cortex neuron differentiation interferon-gamma production skeletal muscle cell differentiation positive regulation of cell differentiation regulation of neuron differentiation positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter cell differentiation involved in embryonic placenta development mesodermal to mesenchymal transition involved in gastrulation

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EOMES Monoclonal Antibody (WD1928), PE-eFluor™ 610, eBioscience™

EOMES Antibody (61-4877-42) in Flow

Product Details

61-4877-42

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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