Invitrogen

FOXP3 Monoclonal Antibody (PCH101), PerCP-Cyanine5.5, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

42 Published Figures

56 References

View all (163) FOXP3 antibodies

Datasheet

Protocols

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Datasheet

Protocols

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FOXP3 Antibody (45-4776-42) in Flow

Surface staining of normal human peripheral blood cells with Anti-Human CD4 APC (Product # 17-0048-42) followed by intracellular staining with Anti-Human Foxp3 PerCP-Cyanine5-5 using the Foxp3/Transcription Factor Staining Buffers (Product # 00-5523-00). Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

FOXP3 Antibody — Intracellular staining of human peripheral blood cells. As expected based on known relative expression patterns, Foxp3 clone PCH101 stains a subset of the CD4+ T cells and does not stain the CD8+ T cells. Details: Normal human peripheral blood cells were surface stained with CD3 (clone UCHT1), CD4 (clone RPA-T4, top), and CD8 (clone OKT8, bottom), followed by intracellular staining with Rat IgG2a kappa Isotype Control (left) or Foxp3 (clone PCH101, right) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD3+ gate were used for analysis. {RE}

Product Details

45-4776-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Flow Cytometry (Flow)

5 µL (0.125 µg)/test

View 55 publications 55 publications

Product Specifications

Species Reactivity

Chimpanzee, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey

Published species

Human

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), PerCP-Cyanine5.5, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

PCH101

Conjugate

PerCP-Cyanine5.5 PerCP-Cyanine5.5 PerCP-Cyanine5.5

Excitation/Emission Max

489/679 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10854725

Product Specific Information

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells.

The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability.

PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, Product # 11-0048-42 , or RPA-T4, Product # 11-0049-42 , depending on the species) and CD25 (BC96, Product # 17-0259-42).

Applications Reported: This PCH101 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This PCH101 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Please refer to Best Protocols for Staining Protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

Excitation: 488 nm; Emission: 695 nm; Laser: Blue Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

In chronic lymphocytic leukemia, activation of the thrombopoietin receptor promotes T-cell inhibitory properties, contributing to immunosuppression.

European journal of haematology

Ringelstein-Harlev S,Fanadka M,Horowitz NA,Bettman NP,Katz T

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Preventive effects of Mycobacterium tuberculosis DNA vaccines on the mouse model with latent tuberculosis infection.

Frontiers in immunology

Liang Y,Li X,Yang Y,Xiao L,Liang Y,Mi J,Xue Y,Gong W,Wang L,Wang J,Zhang J,Shi Y,Peng B,Chen X,Zhao W,Wu X

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Fri Mar 03 00:00:00 EST 2023

AHR/TET2/NT5E axis downregulation is associated with the risk of systemic lupus erythematosus and its progression.

Immunology

Cheng HH,Hung-Ke L,Sheu ML,Lee CY,Tsai YC,Lai DW

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2023

Low-density lipoprotein balances T cell metabolism and enhances response to anti-PD-1 blockade in a HCT116 spheroid model.

Frontiers in oncology

Babl N,Hofbauer J,Matos C,Voll F,Menevse AN,Rechenmacher M,Mair R,Beckhove P,Herr W,Siska PJ,Renner K,Kreutz M,Schnell A

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Tue Feb 14 00:00:00 EST 2023

Aiolos represses CD4⁺ T cell cytotoxic programming via reciprocal regulation of T_FH transcription factors and IL-2 sensitivity.

Nature communications

Read KA,Jones DM,Pokhrel S,Hales EDS,Varkey A,Tuazon JA,Eisele CD,Abdouni O,Saadey A,Leonard MR,Warren RT,Powell MD,Boss JM,Hemann EA,Yount JS,Xin G,Ghoneim HE,Lio CJ,Freud AG,Collins PL,Oestreich KJ

45-4776-42 was used in Flow Cytometry to identify Aiolos as a pivotal regulator of CD4-CTL and TFH programming and highlight its potential as a target for manipulating CD4+ T cell responses.

Fri Mar 24 00:00:00 EDT 2023

FABP4 facilitates inflammasome activation to induce the Treg/Th17 imbalance in preeclampsia via forming a positive feedback with IL-17A.

Molecular therapy. Nucleic acids

Chang GP,Yang XL,Liu W,Lin S,Yang SL,Zhao MY

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Fri Jun 04 00:00:00 EDT 2021

Guidelines for the use of flow cytometry and cell sorting in immunological studies (third edition).

European journal of immunology

Cossarizza A,Chang HD,Radbruch A,Abrignani S,Addo R,Akdis M,Andrä I,Andreata F,Annunziato F,Arranz E,Bacher P,Bari S,Barnaba V,Barros-Martins J,Baumjohann D,Beccaria CG,Bernardo D,Boardman DA,Borger J,Böttcher C,Brockmann L,Burns M,Busch DH,Cameron G,Cammarata I,Cassotta A,Chang Y,Chirdo FG,Christakou E,Čičin-Šain L,Cook L,Corbett AJ,Cornelis R,Cosmi L,Davey MS,De Biasi S,De Simone G,Del Zotto G,Delacher M,Di Rosa F,Di Santo J,Diefenbach A,Dong J,Dörner T,Dress RJ,Dutertre CA,Eckle SBG,Eede P,Evrard M,Falk CS,Feuerer M,Fillatreau S,Fiz-Lopez A,Follo M,Foulds GA,Fröbel J,Gagliani N,Galletti G,Gangaev A,Garbi N,Garrote JA,Geginat J,Gherardin NA,Gibellini L,Ginhoux F,Godfrey DI,Gruarin P,Haftmann C,Hansmann L,Harpur CM,Hayday AC,Heine G,Hernández DC,Herrmann M,Hoelsken O,Huang Q,Huber S,Huber JE,Huehn J,Hundemer M,Hwang WYK,Iannacone M,Ivison SM,Jäck HM,Jani PK,Keller B,Kessler N,Ketelaars S,Knop L,Knopf J,Koay HF,Kobow K,Kriegsmann K,Kristyanto H,Krueger A,Kuehne JF,Kunze-Schumacher H,Kvistborg P,Kwok I,Latorre D,Lenz D,Levings MK,Lino AC,Liotta F,Long HM,Lugli E,MacDonald KN,Maggi L,Maini MK,Mair F,Manta C,Manz RA,Mashreghi MF,Mazzoni A,McCluskey J,Mei HE,Melchers F,Melzer S,Mielenz D,Monin L,Moretta L,Multhoff G,Muñoz LE,Muñoz-Ruiz M,Muscate F,Natalini A,Neumann K,Ng LG,Niedobitek A,Niemz J,Almeida LN,Notarbartolo S,Ostendorf L,Pallett LJ,Patel AA,Percin GI,Peruzzi G,Pinti M,Pockley AG,Pracht K,Prinz I,Pujol-Autonell I,Pulvirenti N,Quatrini L,Quinn KM,Radbruch H,Rhys H,Rodrigo MB,Romagnani C,Saggau C,Sakaguchi S,Sallusto F,Sanderink L,Sandrock I,Schauer C,Scheffold A,Scherer HU,Schiemann M,Schildberg FA,Schober K,Schoen J,Schuh W,Schüler T,Schulz AR,Schulz S,Schulze J,Simonetti S,Singh J,Sitnik KM,Stark R,Starossom S,Stehle C,Szelinski F,Tan L,Tarnok A,Tornack J,Tree TIM,van Beek JJP,van de Veen W,van Gisbergen K,Vasco C,Verheyden NA,von Borstel A,Ward-Hartstonge KA,Warnatz K,Waskow C,Wiedemann A,Wilharm A,Wing J,Wirz O,Wittner J,Yang JHM,Yang J

45-4776-42 was used in Flow cytometry/Cell sorting to provide the key aspects to consider when performing flow cytometry experiments.

Wed Dec 01 00:00:00 EST 2021

Soluble fibrinogen‑like protein 2 levels are decreased in patients with ischemic heart failure and associated with cardiac function.

Molecular medicine reports

You Y,Huang S,Liu H,Fan C,Liu K,Wang Z

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Ex vivo expansion of dysfunctional regulatory T lymphocytes restores suppressive function in Parkinson's disease.

NPJ Parkinson's disease

Thome AD,Atassi F,Wang J,Faridar A,Zhao W,Thonhoff JR,Beers DR,Lai EC,Appel SH

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Thu May 13 00:00:00 EDT 2021

Metabolic Disturbance and Th17/Treg Imbalance Are Associated With Progression of Gingivitis.

Frontiers in immunology

Wang W,Wang X,Lu S,Lv H,Zhao T,Xie G,Du Y,Fan Y,Xu L

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Wed Oct 20 00:00:00 EDT 2021

SARS-CoV-2 infection paralyzes cytotoxic and metabolic functions of the immune cells.

Heliyon

Singh Y,Trautwein C,Fendel R,Krickeberg N,Berezhnoy G,Bissinger R,Ossowski S,Salker MS,Casadei N,Riess O

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Tue Jun 01 00:00:00 EDT 2021

SIRPγ-CD47 Interaction Positively Regulates the Activation of Human T Cells in Situation of Chronic Stimulation.

Frontiers in immunology

Dehmani S,Nerrière-Daguin V,Néel M,Elain-Duret N,Heslan JM,Belarif L,Mary C,Thepenier V,Biteau K,Poirier N,Blancho G,Haspot F

45-4776-42 was used in flow cytometry to show that T-cell interaction with CD47 is of importance notably in chronic stimulation.

Thu Feb 10 00:00:00 EST 2022

Viable bacterial colonization is highly limited in the human intestine in utero.

Nature medicine

Rackaityte E,Halkias J,Fukui EM,Mendoza VF,Hayzelden C,Crawford ED,Fujimura KE,Burt TD,Lynch SV

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Wed Apr 01 00:00:00 EDT 2020

HDAC Inhibitor, CG-745, Enhances the Anti-Cancer Effect of Anti-PD-1 Immune Checkpoint Inhibitor by Modulation of the Immune Microenvironment.

Journal of Cancer

Kim YD,Park SM,Ha HC,Lee AR,Won H,Cha H,Cho S,Cho JM

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Mon Sep 28 00:00:00 EDT 2020

Human Tumor-Infiltrating MAIT Cells Display Hallmarks of Bacterial Antigen Recognition in Colorectal Cancer.

Cell reports. Medicine

Li S,Simoni Y,Becht E,Loh CY,Li N,Lachance D,Koo SL,Lim TP,Tan EKW,Mathew R,Nguyen A,Golovato J,Berkson JD,Prlic M,Lee B,Minot SS,Nagarajan N,Dey N,Tan DSW,Tan IB,Newell EW

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Tue Jun 23 00:00:00 EDT 2020

Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis.

JCI insight

Henderson LA,Hoyt KJ,Lee PY,Rao DA,Jonsson AH,Nguyen JP,Rutherford K,Julé AM,Charbonnier LM,Case S,Chang MH,Cohen EM,Dedeoglu F,Fuhlbrigge RC,Halyabar O,Hazen MM,Janssen E,Kim S,Lo J,Lo MS,Meidan E,Son MBF,Sundel RP,Stoll ML,Nusbaum C,Lederer JA,Chatila TA,Nigrovic PA

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Thu Mar 26 00:00:00 EDT 2020

T-Lymphocyte Subset Distribution and Activity in Patients With Glaucoma.

Investigative ophthalmology & visual science

Yang X,Zeng Q,Göktas E,Gopal K,Al-Aswad L,Blumberg DM,Cioffi GA,Liebmann JM,Tezel G

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Fri Mar 01 00:00:00 EST 2019

Double negative T cells mediate Lag3-dependent antigen-specific protection in allergic asthma.

Nature communications

Tian D,Yang L,Wang S,Zhu Y,Shi W,Zhang C,Jin H,Tian Y,Xu H,Sun G,Liu K,Zhang Z,Zhang D

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Wed Sep 18 00:00:00 EDT 2019

Defective Localization With Impaired Tumor Cytotoxicity Contributes to the Immune Escape of NK Cells in Pancreatic Cancer Patients.

Frontiers in immunology

Lim SA,Kim J,Jeon S,Shin MH,Kwon J,Kim TJ,Im K,Han Y,Kwon W,Kim SW,Yee C,Kim SJ,Jang JY,Lee KM

45-4776-42 was used in Flow Cytometry to investigate NK cell defects within pancreatic ductal adenocarcinoma.

Wed Aug 12 00:00:00 EDT 2020

Fc Effector Function Contributes to the Activity of Human Anti-CTLA-4 Antibodies.

Cancer cell

Arce Vargas F,Furness AJS,Litchfield K,Joshi K,Rosenthal R,Ghorani E,Solomon I,Lesko MH,Ruef N,Roddie C,Henry JY,Spain L,Ben Aissa A,Georgiou A,Wong YNS,Smith M,Strauss D,Hayes A,Nicol D,O'Brien T,Mårtensson L,Ljungars A,Teige I,Frendéus B,Pule M,Marafioti T,Gore M,Larkin J,Turajlic S,Swanton C,Peggs KS,Quezada SA

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Mon Apr 09 00:00:00 EDT 2018

Effect of TGF-β1 on blood CD4⁺CD25^high regulatory T cell proliferation and Foxp3 expression during non-small cell lung cancer blood metastasis.

Experimental and therapeutic medicine

Hu Y,Qi W,Sun L,Zhou H,Zhou B,Yang Z

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Wed Aug 01 00:00:00 EDT 2018

IL-6 receptor blockade corrects defects of XIAP-deficient regulatory T cells.

Nature communications

Hsieh WC,Hsu TS,Chang YJ,Lai MZ

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Wed Jan 31 00:00:00 EST 2018

Challenging PD-L1 expressing cytotoxic T cells as a predictor for response to immunotherapy in melanoma.

Nature communications

Brochez L,Meireson A,Chevolet I,Sundahl N,Ost P,Kruse V

45-4776 was used in Flow cytometry/Cell sorting to study the association of PD-L1-expressing cytotoxic T cells with an altered immune climate and a negative impact on disease outcome in melanoma patients independent of treatment.

Thu Jul 26 00:00:00 EDT 2018

Blocking the recruitment of naive CD4⁺ T cells reverses immunosuppression in breast cancer.

Cell research

Su S,Liao J,Liu J,Huang D,He C,Chen F,Yang L,Wu W,Chen J,Lin L,Zeng Y,Ouyang N,Cui X,Yao H,Su F,Huang JD,Lieberman J,Liu Q,Song E

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Sat Apr 01 00:00:00 EDT 2017

Regulatory T cell subsets in patients with medulloblastoma at diagnosis and during standard irradiation and chemotherapy (PBTC N-11).

Cancer immunology, immunotherapy : CII

Gururangan S,Reap E,Schmittling R,Kocak M,Reynolds R,Grant G,Onar-Thomas A,Baxter P,Pollack IF,Phillips P,Boyett J,Fouladi M,Mitchell D

45-4776 was used in Flow cytometry/Cell sorting to demonstrate that patients with MBL and PFT exhibit overall reduced CD4+ T cell counts at diagnosis but not an elevated proportion of Tregs.

Fri Dec 01 00:00:00 EST 2017

Follicular Regulatory T Cells Are Highly Permissive to R5-Tropic HIV-1.

Journal of virology

Miller SM,Miles B,Guo K,Folkvord J,Meditz AL,McCarter MD,Levy DN,MaWhinney S,Santiago ML,Connick E

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Fri Sep 01 00:00:00 EDT 2017

Alteration of Th17 and Foxp3⁺ regulatory T cells in patients with unexplained recurrent spontaneous abortion before and after the therapy of hCG combined with immunoglobulin.

Experimental and therapeutic medicine

Sha J,Liu F,Zhai J,Liu X,Zhang Q,Zhang B

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Tue Aug 01 00:00:00 EDT 2017

HDAC inhibition potentiates immunotherapy in triple negative breast cancer.

Oncotarget

Terranova-Barberio M,Thomas S,Ali N,Pawlowska N,Park J,Krings G,Rosenblum MD,Budillon A,Munster PN

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Tue Dec 26 00:00:00 EST 2017

Serial immunomonitoring of cancer patients receiving combined antagonistic anti-CD40 and chemotherapy reveals consistent and cyclical modulation of T cell and dendritic cell parameters.

BMC cancer

McDonnell AM,Cook A,Robinson BWS,Lake RA,Nowak AK

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Thu Jun 15 00:00:00 EDT 2017

Oxygen Sensing by T Cells Establishes an Immunologically Tolerant Metastatic Niche.

Cell

Clever D,Roychoudhuri R,Constantinides MG,Askenase MH,Sukumar M,Klebanoff CA,Eil RL,Hickman HD,Yu Z,Pan JH,Palmer DC,Phan AT,Goulding J,Gattinoni L,Goldrath AW,Belkaid Y,Restifo NP

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Thu Aug 25 00:00:00 EDT 2016

Pulmonary sarcoidosis is associated with high-level inducible co-stimulator (ICOS) expression on lung regulatory T cells--possible implications for the ICOS/ICOS-ligand axis in disease course and resolution.

Clinical and experimental immunology

Sakthivel P,Grunewald J,Eklund A,Bruder D,Wahlström J

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Human Head and Neck Squamous Cell Carcinoma-Associated Semaphorin 4D Induces Expansion of Myeloid-Derived Suppressor Cells.

Journal of immunology (Baltimore, Md. : 1950)

Younis RH,Han KL,Webb TJ

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Mon Feb 01 00:00:00 EST 2016

Development of Type 2, But Not Type 1, Leprosy Reactions is Associated with a Severe Reduction of Circulating and In situ Regulatory T-Cells.

The American journal of tropical medicine and hygiene

Vieira AP,Trindade MÂ,Pagliari C,Avancini J,Sakai-Valente NY,Duarte AJ,Benard G

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Fri Apr 01 00:00:00 EDT 2016

Equilibrium of Treg/Th17 cells of peripheral blood in syphilitic patients with sero-resistance.

Experimental and therapeutic medicine

Zhao J,Ma J,Zhang X,Li Q,Yang X

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Wed Jun 01 00:00:00 EDT 2016

Glycolysis controls the induction of human regulatory T cells by modulating the expression of FOXP3 exon 2 splicing variants.

Nature immunology

De Rosa V,Galgani M,Porcellini A,Colamatteo A,Santopaolo M,Zuchegna C,Romano A,De Simone S,Procaccini C,La Rocca C,Carrieri PB,Maniscalco GT,Salvetti M,Buscarinu MC,Franzese A,Mozzillo E,La Cava A,Matarese G

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Sun Nov 01 00:00:00 EDT 2015

CTLA-4⁺ Regulatory T Cells Increased in Cetuximab-Treated Head and Neck Cancer Patients Suppress NK Cell Cytotoxicity and Correlate with Poor Prognosis.

Cancer research

Jie HB,Schuler PJ,Lee SC,Srivastava RM,Argiris A,Ferrone S,Whiteside TL,Ferris RL

45-4776 was used in Flow cytometry/Cell sorting to demonstrate that differences in Treg-mediated suppression contribute to the clinical response to cetuximab treatment.

Mon Jun 01 00:00:00 EDT 2015

Statins increase the frequency of circulating CD4+ FOXP3+ regulatory T cells in healthy individuals.

Journal of immunology research

Rodríguez-Perea AL,Montoya CJ,Olek S,Chougnet CA,Velilla PA

45-4776 was used in Flow cytometry/Cell sorting to investigate the effect of statins on Tregs in the absence of inflammation, showing that they increase the frequency of CD4(+)FOXP3(+) Tregs.

Tue Dec 01 00:00:00 EST 2015

Characterization of the in vivo immune network of IDO, tryptophan metabolism, PD-L1, and CTLA-4 in circulating immune cells in melanoma.

Oncoimmunology

Chevolet I,Speeckaert R,Schreuer M,Neyns B,Krysko O,Bachert C,Hennart B,Allorge D,van Geel N,Van Gele M,Brochez L

45-4776 was used in Flow cytometry/Cell sorting to demonstrate that the expression of IDO, PD-L1, and CTLA-4 in the peripheral blood of melanoma patients is strongly interconnected.

Sun Mar 01 00:00:00 EST 2015

Clinical significance of plasmacytoid dendritic cells and myeloid-derived suppressor cells in melanoma.

Journal of translational medicine

Chevolet I,Speeckaert R,Schreuer M,Neyns B,Krysko O,Bachert C,Van Gele M,van Geel N,Brochez L

45-4776 was used in Flow cytometry/Cell sorting to investigate multiple immune cell types in a series of blood samples from melanoma patients with different stages of the disease.

Fri Jan 16 00:00:00 EST 2015

Antigen-specific expansion of human regulatory T cells as a major tolerance mechanism against mucosal fungi.

Mucosal immunology

Bacher P,Kniemeyer O,Schönbrunn A,Sawitzki B,Assenmacher M,Rietschel E,Steinbach A,Cornely OA,Brakhage AA,Thiel A,Scheffold A

45-4776 was used in Flow cytometry/Cell sorting to compare the conventional vs. regulatory CD4(+) T-cell response directed against Aspergillus fumigatus and Candida albicans.

Tue Jul 01 00:00:00 EDT 2014

Delta-like 1-mediated Notch signaling enhances the in vitro conversion of human memory CD4 T cells into FOXP3-expressing regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Mota C,Nunes-Silva V,Pires AR,Matoso P,Victorino RM,Sousa AE,Caramalho I

45-4776 was used in Flow cytometry/Cell sorting to investigate novel strategies to generate in vitro-induced Tregs.

Mon Dec 15 00:00:00 EST 2014

Identification of immunogenic antigens from Aspergillus fumigatus by direct multiparameter characterization of specific conventional and regulatory CD4+ T cells.

Journal of immunology (Baltimore, Md. : 1950)

Bacher P,Kniemeyer O,Teutschbein J,Thön M,Vödisch M,Wartenberg D,Scharf DH,Koester-Eiserfunke N,Schütte M,Dübel S,Assenmacher M,Brakhage AA,Scheffold A

45-4776 was used in Flow cytometry/Cell sorting to characterise A. fumigatus-specific CD4(+) T cells for rapid identification of physiological T cell targets in healthy donors.

Wed Oct 01 00:00:00 EDT 2014

Human T cells upregulate CD69 after coculture with xenogeneic genetically-modified pig mesenchymal stromal cells.

Cellular immunology

Li J,Andreyev O,Chen M,Marco M,Iwase H,Long C,Ayares D,Shen Z,Cooper DK,Ezzelarab MB

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Mon Jan 20 00:00:00 EST 2014

Impaired function of regulatory T cells in cord blood of children of allergic mothers.

Clinical and experimental immunology

Hrdý J,Kocourková I,Prokešová L

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Mon Oct 01 00:00:00 EDT 2012

OMIP-006: phenotypic subset analysis of human T regulatory cells via polychromatic flow cytometry.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Murdoch DM,Staats JS,Weinhold KJ

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Sun Apr 01 00:00:00 EDT 2012

Follicular regulatory T cells expressing Foxp3 and Bcl-6 suppress germinal center reactions.

Nature medicine

Chung Y,Tanaka S,Chu F,Nurieva RI,Martinez GJ,Rawal S,Wang YH,Lim H,Reynolds JM,Zhou XH,Fan HM,Liu ZM,Neelapu SS,Dong C

45-4776 was used in in vitro experiments to identify the Bcl6+CXCR5+Foxp3+ T cell as a distinct effector Treg subset specialised for controlling germinal center reactions in mice and humans.

Sun Jul 24 00:00:00 EDT 2011

Gut derived lactic acid bacteria induce strain specific CD4(+) T cell responses in human PBMC.

Clinical nutrition (Edinburgh, Scotland)

de Roock S,van Elk M,Hoekstra MO,Prakken BJ,Rijkers GT,de Kleer IM

45-4776 was used in Flow cytometry/Cell sorting to investigate the immune influencing capacity of 19 bacterial strains of commonly used species using a high throughput method, select potential strains for future clinical trials.

Thu Dec 01 00:00:00 EST 2011

Angiopoietin 2 stimulates TIE2-expressing monocytes to suppress T cell activation and to promote regulatory T cell expansion.

Journal of immunology (Baltimore, Md. : 1950)

Coffelt SB,Chen YY,Muthana M,Welford AF,Tal AO,Scholz A,Plate KH,Reiss Y,Murdoch C,De Palma M,Lewis CE

45-4776 was used in Flow cytometry/Cell sorting to suggest that ANGPT2-stimulated TIE2-expressing monocytes/macrophages represent a novel, potent immunosuppressive force in tumours.

Fri Apr 01 00:00:00 EDT 2011

Engagement of TLR2 reverses the suppressor function of conjunctiva CD4+CD25+ regulatory T cells and promotes herpes simplex virus epitope-specific CD4+CD25- effector T cell responses.

Investigative ophthalmology & visual science

Dasgupta G,Chentoufi AA,You S,Falatoonzadeh P,Urbano LA,Akhtarmalik A,Nguyen K,Ablabutyan L,Nesburn AB,BenMohamed L

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Tue May 17 00:00:00 EDT 2011

CD40 signalling induces IL-10-producing, tolerogenic dendritic cells.

Experimental dermatology

Tuettenberg A,Fondel S,Steinbrink K,Enk AH,Jonuleit H

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Fri Jan 01 00:00:00 EST 2010

Safety and T cell modulating effects of high dose vitamin D3 supplementation in multiple sclerosis.

PloS one

Smolders J,Peelen E,Thewissen M,Cohen Tervaert JW,Menheere P,Hupperts R,Damoiseaux J

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Mon Dec 13 00:00:00 EST 2010

Fc receptor-like 3 protein expressed on IL-2 nonresponsive subset of human regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Nagata S,Ise T,Pastan I

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Mon Jun 15 00:00:00 EDT 2009

IL-2 administration increases CD4+ CD25(hi) Foxp3+ regulatory T cells in cancer patients.

Blood

Ahmadzadeh M,Rosenberg SA

Published figure using FOXP3 monoclonal antibody (Product # 45-4776-42) in Flow Cytometry

Wed Mar 15 00:00:00 EST 2006

Mucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART.

Blood

Epple HJ,Loddenkemper C,Kunkel D,Tröger H,Maul J,Moos V,Berg E,Ullrich R,Schulzke JD,Stein H,Duchmann R,Zeitz M,Schneider T

Wed Nov 01 00:00:00 EST 2006

Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Hillsamer P,Banham AH,Kim CH

Sat Oct 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; regulatory protein Foxp3; RP23-54C14.1; Scurfin

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Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; XPID

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UniProt ID: (Human) Q9BZS1, (Cynomolgus monkey) Q6U8D7

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Entrez Gene ID: (Human) 50943, (Cynomolgus monkey) 102120882, (Rhesus monkey) 574303

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NF-kappaB binding NFAT protein binding

Process(es)

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FOXP3 Monoclonal Antibody (PCH101), PerCP-Cyanine5.5, eBioscience™

FOXP3 Antibody (45-4776-42) in Flow

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45-4776-42

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Flow Cytometry (Flow)

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