Invitrogen

FOXP3 Monoclonal Antibody (PCH101), eFluor™ 450, eBioscience™

Advanced Verification

This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.

24 References

View all (163) FOXP3 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Advanced Verification (1)

FIGURE: 1 / 2

FOXP3 Antibody (48-4776-42) in Flow

Surface staining of normal human peripheral blood cells with Anti-Human CD4 PE (Product # 12-0049-42) (left) or Anti-Human CD25 APC (Product # 17-0259-42) (right) followed by fixation and permeabilization with the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and intracellular staining with Anti-Human Foxp3 eFluor® 450. Cells in the lymphocyte gate were used for analysis.

Product Image Gallery

FOXP3 Antibody — Intracellular staining of human peripheral blood cells. As expected based on known relative expression patterns, Foxp3 clone PCH101 stains a subset of the CD4+ T cells and does not stain the CD8+ T cells. Details: Normal human peripheral blood cells were surface stained with CD3 (clone UCHT1), CD4 (clone RPA-T4, top), and CD8 (clone OKT8, bottom), followed by intracellular staining with Rat IgG2a kappa Isotype Control (left) or Foxp3 (clone PCH101, right) using the Foxp3/Transcription Factor Staining Buffer Set and protocol. Lymphocytes in the CD3+ gate were used for analysis. {RE}

Product Details

48-4776-42

Applications

Tested Dilution

Publications

Immunohistochemistry (Paraffin) (IHC (P))

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

5 µL (0.5 µg)/test

View 21 publications 21 publications

Product Specifications

Species Reactivity

Chimpanzee, Cynomolgus monkey, Human, Non-human primate, Rhesus monkey

Published species

Human

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

PCH101

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

5 µL/Test

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2, with 0.2% BSA

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_1834364

Product Specific Information

Description: eBioscience offers a panel of monoclonal antibodies to different epitopes of human Foxp3, providing useful tools for investigating the complete expression pattern of Foxp3 at the protein level, and discerning the precise subsets of Foxp3^+ cells.

The PCH101 antibody reacts with the amino terminus of human foxp3 protein also known as FORKHEAD BOX P3, SCURFIN, and JM2; cross reactivity of this antibody to other proteins has not been determined. Foxp3, a 49-55 kDa protein, is a member of the forkhead/winged-helix family of transcriptional regulators, and was identified as the gene defective in 'scurfy' (sf) mice. Constitutive high expression of Foxp3 mRNA has been shown in CD4+CD25+ regulatory T cells (Treg cells), and ectopic expression of foxp3 in CD4+CD25- cells imparts a Treg phenotype in these cells.

Intracellular staining of human peripheral blood mononuclear cells (PBMCs) with PCH101 antibody using the anti-human Foxp3 Staining Set and protocol reveals approximately 0.5-4% of lymphocytes staining, with the majority of staining occurring in the CD25^bright population. This is subject to donor variability.

PCH101 crossreacts with rhesus, chimpanzee and cynomolgus. We recommend the use of CD4 (OKT4, Product # 11-0048-42 , or RPA-T4, Product # 11-0049-42 , depending on the species) and CD25 (BC96, Product # 17-0259-42).

Applications Reported: This PCH101 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This PCH101 antibody has been pre-titrated and tested by intracellular staining and by flow cytometric analysis using the Foxp3/Transcription Factor Staining Buffer Set () and protocol. Please see Best Protocols Section (Staining intracellular Antigens for Flow Cytometry) for staining protocol (refer to Protocol B: One-step protocol for intracellular (nuclear) proteins). This antibody can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

FOXP3 (Forkhead box protein 3) is a member of the forkhead/winged-helix family of transcriptional regulators, highly conserved across mammals, and essential for normal immune homeostasis. FOXP3 is 381 amino acids long, stably and constitutively expressed at a high level in CD25 + CD4 positive regulatory T cells, a low level in CD4-positive/CD25-negative cells, and is absent in CD4-negative/CD8-positive T cells. FOXP3 may be a master regulatory gene, and a more specific marker of regulatory T cells. Defects in the gene encoding FOXP3 protein cause the scurfy phenotype in mice. In humans FOXP3 defects play a role in IPEX syndrome (immune dysfunction, polyendocrinopathy, enteropathy, X-linked syndrome), also known as X-linked autoimmunity-allergic dysregulation (XLAAD) syndrome. Transcript variants of FOXP3 encoding different isoforms have been identified. In human breast and colon cancer cells, expression of FOXP3 is regulated by p53 in response to the DNA damage.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Neoantigen-specific CD4⁺ T cells in human melanoma have diverse differentiation states and correlate with CD8⁺ T cell, macrophage, and B cell function.

Cancer cell

Veatch JR,Lee SM,Shasha C,Singhi N,Szeto JL,Moshiri AS,Kim TS,Smythe K,Kong P,Fitzgibbon M,Jesernig B,Bhatia S,Tykodi SS,Hall ET,Byrd DR,Thompson JA,Pillarisetty VG,Duhen T,McGarry Houghton A,Newell E,Gottardo R,Riddell SR

48-4776-42 was used in Immunocytochemistry to identify phenotypes and functional correlates of tumor-specific CD4+ T cells in melanoma and suggest the possibility of using such cells to modify the tumor microenvironment.

Mon Apr 11 00:00:00 EDT 2022

Tissue-resident-like CD4+ T cells secreting IL-17 control Mycobacterium tuberculosis in the human lung.

The Journal of clinical investigation

Ogongo P,Tezera LB,Ardain A,Nhamoyebonde S,Ramsuran D,Singh A,Ng'oepe A,Karim F,Naidoo T,Khan K,Dullabh KJ,Fehlings M,Lee BH,Nardin A,Lindestam Arlehamn CS,Sette A,Behar SM,Steyn AJ,Madansein R,Kløverpris HN,Elkington PT,Leslie A

48-4776-42 was used in Flow Cytometry to examine M. tuberculosis-specific lung tissue-resident memory T cells from humans.

Mon May 17 00:00:00 EDT 2021

People critically ill with COVID-19 exhibit peripheral immune profiles predictive of mortality and reflective of SARS-CoV-2 lung viral burden.

Cell reports. Medicine

Cillo AR,Somasundaram A,Shan F,Cardello C,Workman CJ,Kitsios GD,Ruffin AT,Kunning S,Lampenfeld C,Onkar S,Grebinoski S,Deshmukh G,Methe B,Liu C,Nambulli S,Andrews LP,Duprex WP,Joglekar AV,Benos PV,Ray P,Ray A,McVerry BJ,Zhang Y,Lee JS,Das J,Singh H,Morris A,Bruno TC,Vignali DAA

48-4776-42 was used in Flow cytometry/Cell sorting to present high-dimensional profiling of blood and respiratory samples from people with severe COVID-19 to examine the association between cell-linked molecular features and mortality outcomes.

Tue Dec 21 00:00:00 EST 2021

Immune Landscape of Viral- and Carcinogen-Driven Head and Neck Cancer.

Immunity

Cillo AR,Kürten CHL,Tabib T,Qi Z,Onkar S,Wang T,Liu A,Duvvuri U,Kim S,Soose RJ,Oesterreich S,Chen W,Lafyatis R,Bruno TC,Ferris RL,Vignali DAA

48-4776 was used in Flow cytometry/Cell sorting to assess the transcriptional profiles of 131,224 single cells from peripheral and intra-tumoral immune populations from patients with HPV- and HPV+ HNSCC and healthy donors.

Tue Jan 14 00:00:00 EST 2020

A regulatory T cell Notch4-GDF15 axis licenses tissue inflammation in asthma.

Nature immunology

Harb H,Stephen-Victor E,Crestani E,Benamar M,Massoud A,Cui Y,Charbonnier LM,Arbag S,Baris S,Cunnigham A,Leyva-Castillo JM,Geha RS,Mousavi AJ,Guennewig B,Schmitz-Abe K,Sioutas C,Phipatanakul W,Chatila TA

48-4776 was used in Flow cytometry/Cell sorting to find that interleukin-6- and STAT3 transcription factor-dependent upregulation of Notch4 receptor on lung tissue regulatory T (Treg) cells is necessary for allergens and particulate matter pollutants to promote airway inflammation.

Sun Nov 01 00:00:00 EDT 2020

Clusters of Tolerogenic B Cells Feature in the Dynamic Immunological Landscape of the Pregnant Uterus.

Cell reports

Benner M,Feyaerts D,García CC,Inci N,López SC,Fasse E,Shadmanfar W,van der Heijden OWH,Gorris MAJ,Joosten I,Ferwerda G,van der Molen RG

48-4776-42 was used in Flow Cytometry to suggest a role for B cells in healthy pregnancy.

Tue Sep 29 00:00:00 EDT 2020

Th17 reprogramming of T cells in systemic juvenile idiopathic arthritis.

JCI insight

Henderson LA,Hoyt KJ,Lee PY,Rao DA,Jonsson AH,Nguyen JP,Rutherford K,Julé AM,Charbonnier LM,Case S,Chang MH,Cohen EM,Dedeoglu F,Fuhlbrigge RC,Halyabar O,Hazen MM,Janssen E,Kim S,Lo J,Lo MS,Meidan E,Son MBF,Sundel RP,Stoll ML,Nusbaum C,Lederer JA,Chatila TA,Nigrovic PA

48-4776 was used in Flow cytometry/Cell sorting to show how systemic juvenile idiopathic arthritis (sJIA) transitions from acute to chronic through the evolving Th17 polarization that begins in Tregs and progresses to T effector cells (Teffs).

Thu Mar 26 00:00:00 EDT 2020

50-Gy Stereotactic Body Radiation Therapy to the Dominant Intraprostatic Nodule: Results From a Phase 1a/b Trial.

International journal of radiation oncology, biology, physics

Herrera FG,Valerio M,Berthold D,Tawadros T,Meuwly JY,Vallet V,Baumgartner P,Thierry AC,De Bari B,Jichlinski P,Kandalaft L,Coukos G,Harari A,Bourhis J

48-4776-42 was used in Flow Cytometry to show stereotactic irradiation induced antigen-specific immune responses in a subset of patients.

Fri Feb 01 00:00:00 EST 2019

Niraparib activates interferon signaling and potentiates anti-PD-1 antibody efficacy in tumor models.

Scientific reports

Wang Z,Sun K,Xiao Y,Feng B,Mikule K,Ma X,Feng N,Vellano CP,Federico L,Marszalek JR,Mills GB,Hanke J,Ramaswamy S,Wang J

48-4776 was used in Flow cytometry/Cell sorting to investigate the therapeutic potential of combining niraparib with anti-P-1 immune checkpoint inhibitors in preclinical tumour models.

Tue Feb 12 00:00:00 EST 2019

Regulatory T cells use arginase 2 to enhance their metabolic fitness in tissues.

JCI insight

Lowe MM,Boothby I,Clancy S,Ahn RS,Liao W,Nguyen DN,Schumann K,Marson A,Mahuron KM,Kingsbury GA,Liu Z,Munoz Sandoval P,Rodriguez RS,Pauli ML,Taravati K,Arron ST,Neuhaus IM,Harris HW,Kim EA,Shin US,Krummel MF,Daud A,Scharschmidt TC,Rosenblum MD

48-4776 was used in Flow cytometry/Cell sorting to compare healthy skin Tregs with those in peripheral blood, inflamed psoriatic skin, and metastatic melanoma.

Thu Dec 19 00:00:00 EST 2019

Non-conventional Inhibitory CD4⁺Foxp3^-PD-1^hi T Cells as a Biomarker of Immune Checkpoint Blockade Activity.

Cancer cell

Zappasodi R,Budhu S,Hellmann MD,Postow MA,Senbabaoglu Y,Manne S,Gasmi B,Liu C,Zhong H,Li Y,Huang AC,Hirschhorn-Cymerman D,Panageas KS,Wherry EJ,Merghoub T,Wolchok JD

48-4776-42 was used in Flow Cytometry to provide evidence that mouse and human circulating and intra-tumor 4PD1hi inhibit T cell functions in a PD-1/PD-L1 dependent fashion and resemble follicular helper T cell (TFH)-like cells.

Mon Jun 11 00:00:00 EDT 2018

An Autocrine TNFα-Tumor Necrosis Factor Receptor 2 Loop Promotes Epigenetic Effects Inducing Human Treg Stability In Vitro.

Frontiers in immunology

Urbano PCM,Koenen HJPM,Joosten I,He X

48-4776 was used in Flow Cytometry to explore the underlying mechanisms of rapamycin and tumour necrosis factor receptor 2 agonist-mediated regulatory T cell stability.

Tue May 28 00:00:00 EDT 2019

DNA Methyltransferase Inhibition Promotes Th1 Polarization in Human CD4⁺CD25^high FOXP3⁺ Regulatory T Cells but Does Not Affect Their Suppressive Capacity.

Journal of immunology research

Landman S,Cruijsen M,Urbano PCM,Huls G,van Erp PEJ,van Rijssen E,Joosten I,Koenen HJPM

48-4776 was used in Flow cytometry/Cell sorting to examine the in vitro effect of derivative 5-aza-2'-deoxycytidine on the stability and function of FACS-sorted human naturally occurring CD4+CD25high FOXP3+ regulatory T cells.

Thu Oct 11 00:00:00 EDT 2018

RNA-seq and flow-cytometry of conventional, scalp, and palmoplantar psoriasis reveal shared and distinct molecular pathways.

Scientific reports

Ahn R,Yan D,Chang HW,Lee K,Bhattarai S,Huang ZM,Nakamura M,Singh R,Afifi L,Taravati K,Munoz-Sandoval P,Pauli M,Rosenblum MD,Liao W

Fri Jul 27 00:00:00 EDT 2018

Intradermal injection of low dose human regulatory T cells inhibits skin inflammation in a humanized mouse model.

Scientific reports

Landman S,de Oliveira VL,van Erp PEJ,Fasse E,Bauland SCG,Joosten I,Koenen HJPM

48-4776 was used in Flow cytometry/Cell sorting to evaluate if local delivery of low numbers of human regulatory T cells by intradermal injection prevents skin inflammation in the humanised mouse huPBL-SCID-huSkin allograft model.

Tue Jul 03 00:00:00 EDT 2018

PEGylated IL-10 (Pegilodecakin) Induces Systemic Immune Activation, CD8⁺ T Cell Invigoration and Polyclonal T Cell Expansion in Cancer Patients.

Cancer cell

Naing A,Infante JR,Papadopoulos KP,Chan IH,Shen C,Ratti NP,Rojo B,Autio KA,Wong DJ,Patel MR,Ott PA,Falchook GS,Pant S,Hung A,Pekarek KL,Wu V,Adamow M,McCauley S,Mumm JB,Wong P,Van Vlasselaer P,Leveque J,Tannir NM,Oft M

48-4776-42 was used in Flow cytometry/Cell sorting to report that pegilodecakin induces hallmarks of CD8+ T cell immunity in cancer patients, including elevation of interferon-γ and GranzymeB, expansion and activation of intratumoral CD8+ T cells, and proliferation and expansion of LAG-3+ PD-1+ CD8+ T cells.

Mon Nov 12 00:00:00 EST 2018

Phenotypic Complexity of the Human Regulatory T Cell Compartment Revealed by Mass Cytometry.

Journal of immunology (Baltimore, Md. : 1950)

Mason GM,Lowe K,Melchiotti R,Ellis R,de Rinaldis E,Peakman M,Heck S,Lombardi G,Tree TI

48-4776 was used in Flow cytometry/Cell sorting to examine the complexity of human Tregs using an extensive panel of surface markers associated with Treg function and phenotype.

Tue Sep 01 00:00:00 EDT 2015

Genetic analysis of an allergic rhinitis cohort reveals an intercellular epistasis between FAM134B and CD39.

BMC medical genetics

Melchiotti R,Puan KJ,Andiappan AK,Poh TY,Starke M,Zhuang L,Petsch K,Lai TS,Chew FT,Larbi A,Wang de Y,Poidinger M,Rotzschke O

48-4776 was used in Flow cytometry/Cell sorting to identify variants affecting CD39 expression on regulatory T cells, and evaluate their association with allergic rhinitis.

Fri Jun 27 00:00:00 EDT 2014

A functional variant in FCRL3 is associated with higher Fc receptor-like 3 expression on T cell subsets and rheumatoid arthritis disease activity.

Arthritis and rheumatism

Bajpai UD,Swainson LA,Mold JE,Graf JD,Imboden JB,McCune JM

48-4776 was used in Flow cytometry/Cell sorting to indicate that Fc receptor-like 3 (FCRL3) expression, associated with the FCRL3-169C allele, may be a biomarker for rheumatoid arthritis disease activity.

Wed Aug 01 00:00:00 EDT 2012

Expression of the autoimmune susceptibility gene FcRL3 on human regulatory T cells is associated with dysfunction and high levels of programmed cell death-1.

Journal of immunology (Baltimore, Md. : 1950)

Swainson LA,Mold JE,Bajpai UD,McCune JM

48-4776 was used in Flow cytometry/Cell sorting to suggest that FcRL3 expression is associated with T(reg) dysfunction that may, in turn, contribute to the loss of self-tolerance and the development of autoimmunity.

Thu Apr 01 00:00:00 EDT 2010

Protection against bronchiolitis obliterans syndrome is associated with allograft CCR7+ CD45RA- T regulatory cells.

PloS one

Gregson AL,Hoji A,Palchevskiy V,Hu S,Weigt SS,Liao E,Derhovanessian A,Saggar R,Song S,Elashoff R,Yang OO,Belperio JA

48-4776 was used in Flow cytometry/Cell sorting to hypothesize that frequencies of Treg in bronchoalveolar lavage fluid after lung transplantation would predict subsequent development of bronchiolitis obliterans syndrome.

Tue Jun 29 00:00:00 EDT 2010

Protection against bronchiolitis obliterans syndrome is associated with allograft CCR7+ CD45RA- T regulatory cells.

PloS one

Gregson AL,Hoji A,Palchevskiy V,Hu S,Weigt SS,Liao E,Derhovanessian A,Saggar R,Song S,Elashoff R,Yang OO,Belperio JA

Tue Jun 29 00:00:00 EDT 2010

Mucosal but not peripheral FOXP3+ regulatory T cells are highly increased in untreated HIV infection and normalize after suppressive HAART.

Blood

Epple HJ,Loddenkemper C,Kunkel D,Tröger H,Maul J,Moos V,Berg E,Ullrich R,Schulzke JD,Stein H,Duchmann R,Zeitz M,Schneider T

Wed Nov 01 00:00:00 EST 2006

Cutting edge: direct suppression of B cells by CD4+ CD25+ regulatory T cells.

Journal of immunology (Baltimore, Md. : 1950)

Lim HW,Hillsamer P,Banham AH,Kim CH

Sat Oct 01 00:00:00 EDT 2005

Bioinformatics

Protein Aliases: Forkhead box protein P3; FOXP3delta7; immune dysregulation, polyendocrinopathy, enteropathy, X-linked; immunodeficiency, polyendocrinopathy, enteropathy, X-linked; MGC141961; MGC141963; regulatory protein Foxp3; RP23-54C14.1; Scurfin

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Gene Aliases: AIID; DIETER; FOXP3; IPEX; JM2; PIDX; XPID

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UniProt ID: (Human) Q9BZS1, (Cynomolgus monkey) Q6U8D7

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Entrez Gene ID: (Human) 50943, (Cynomolgus monkey) 102120882, (Rhesus monkey) 574303

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Function(s)

RNA polymerase II transcription factor activity, sequence-specific DNA binding core promoter binding transcription factor activity, sequence-specific DNA binding transcription factor activity, RNA polymerase II distal enhancer sequence-specific binding transcription corepressor activity protein binding histone acetyltransferase binding protein homodimerization activity histone deacetylase binding sequence-specific DNA binding metal ion binding NF-kappaB binding NFAT protein binding

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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FOXP3 Monoclonal Antibody (PCH101), eFluor™ 450, eBioscience™

FOXP3 Antibody (48-4776-42) in Flow

Product Details

48-4776-42

Immunohistochemistry (Paraffin) (IHC (P))

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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