Invitrogen

IL-12/IL-23 p40 Monoclonal Antibody (C17.8), Biotin, eBioscience™

Tech Support

Datasheet

Protocols

Questions & Answers

Tech Support

Published Figures (5)

IL-12/IL-23 p40 Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 5

IL-12/IL-23 p40 Antibody (13-7123-81) in Flow

Nature communications 2018 - Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 13-7123-81) in Flow Cytometry

IL-12/IL-23 p40 Antibody in Neutralization (Neu)

Product Image Gallery

Product Details

13-7123-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

View 3 publications 3 publications

ELISA (ELISA)

0.5-2 µg/mL

View 9 publications 9 publications

Neutralization (Neu)

View 1 publication 1 publication

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

C17.8

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466928

Product Specific Information

Description: The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells.

Applications Reported: The C17.8 antibody has been reported for use in IP, Western, ELISA, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested: The biotinylated C17.8 antibody has been tested as the detection antibody in three different sandwich ELISAs, for measurement of mouse IL-12 p70, mouse IL-23, or mouse IL-12 p40 total.

For mouse IL-12 p70 ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in sandwich ELISA for analysis of mouse IL-12 p70, in combination with the purified C18.2 antibody (14-7122) for capture and recombinant mouse IL-12 p70 (39-8121) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2,000 pg/mL - 15 pg/mL should be included in each ELISA plate. Super AquaBlue or ABTS are suitable substrates.

For mouse IL-23 ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-23 protein levels, in combination with the purified G23-8 antibody (14-7232) for capture and recombinant mouse IL-23 (39-8231) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.25 - 1 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4,000 pg/mL - 30 pg/mL should be included in each ELISA plate. Note: TMB (00-4201), rather than ABTS, should be used as the substrate.

For mouse IL-12 p40 Total ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse total p40 (e.g., p40 monomer, p40 dimer, p40/p35, and p40/p19), in combination with the purified C15.6 antibody (14-7125) for capture and recombinant mouse IL-12 p40 (39-8122), IL-12 p70 (39-8121) or mouse IL-23 (39-8231) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2,000 pg/mL - 15 pg/mL should be included in each ELISA plate. Super AquaBlue or ABTS are suitable substrates.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Requirements for the differentiation of innate T-bet^high memory-phenotype CD4⁺ T lymphocytes under steady state.

Nature communications

Kawabe T,Yi J,Kawajiri A,Hilligan K,Fang D,Ishii N,Yamane H,Zhu J,Jankovic D,Kim KS,Trinchieri G,Sher A

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 13-7123-81) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Microbiota Sensing by Mincle-Syk Axis in Dendritic Cells Regulates Interleukin-17 and -22 Production and Promotes Intestinal Barrier Integrity.

Immunity

Martínez-López M,Iborra S,Conde-Garrosa R,Mastrangelo A,Danne C,Mann ER,Reid DM,Gaboriau-Routhiau V,Chaparro M,Lorenzo MP,Minnerup L,Saz-Leal P,Slack E,Kemp B,Gisbert JP,Dzionek A,Robinson MJ,Rupérez FJ,Cerf-Bensussan N,Brown GD,Bernardo D,LeibundGut-Landmann S,Sancho D

13-7123-81 was used in ELISA to conclude that sensing of commensals by Mincle and Syk signaling in CD11c+ cells reinforces intestinal immune barrier and promotes host-microbiota mutualism, preventing systemic inflammation.

Tue Feb 19 00:00:00 EST 2019

Absence of GdX/UBL4A Protects against Inflammatory Diseases by Regulating NF-кB Signaling in Macrophages and Dendritic Cells.

Theranostics

Liu C,Zhou Y,Li M,Wang Y,Yang L,Yang S,Feng Y,Wang Y,Wang Y,Ren F,Li J,Dong Z,Chin YE,Fu X,Wu L,Chang Z

13-7123 was used in an ELISA assay to investigate whether ubiquitin-like protein 4A has a role during the innate immune response.

Fri Jan 31 00:00:00 EST 2020

p38α has an important role in antigen cross-presentation by dendritic cells.

Cellular & molecular immunology

Zhou Y,Wu J,Liu C,Guo X,Zhu X,Yao Y,Jiao Y,He P,Han J,Wu L

13-7123 was used in an ELISA assay to study whether the p38 signalling pathway affects the important antigen presentation function of dendritic cells.

Thu Mar 01 00:00:00 EST 2018

Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype.

Nature communications

Chen D,Xie J,Fiskesund R,Dong W,Liang X,Lv J,Jin X,Liu J,Mo S,Zhang T,Cheng F,Zhou Y,Zhang H,Tang K,Ma J,Liu Y,Huang B

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 13-7123-81) in Flow Cytometry

Wed Feb 28 00:00:00 EST 2018

Analysis of the Trichuris suis excretory/secretory proteins as a function of life cycle stage and their immunomodulatory properties.

Scientific reports

Leroux LP,Nasr M,Valanparambil R,Tam M,Rosa BA,Siciliani E,Hill DE,Zarlenga DS,Jaramillo M,Weinstock JV,Geary TG,Stevenson MM,Urban JF,Mitreva M,Jardim A

13-7123 was used in an ELISA assay to analyse the Trichuris suis excretory/secretory proteins as a function of life cycle stage and their immunomodulatory properties.

Mon Oct 29 00:00:00 EDT 2018

CD1b-autoreactive T cells recognize phospholipid antigens and contribute to antitumor immunity against a CD1b⁺ T cell lymphoma.

Oncoimmunology

Bagchi S,Li S,Wang CR

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 13-7123-81) in Neutralization

Tue Jan 12 00:00:00 EST 2021

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 13-7123-81) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

Intestinal bacteria induce TSLP to promote mutualistic T-cell responses.

Mucosal immunology

Mosconi I,Geuking MB,Zaiss MM,Massacand JC,Aschwanden C,Kwong Chung CK,McCoy KD,Harris NL

13-7123 was used in an ELISA assay to show that steady-state thymic stromal lymphopoietin expression requires intestinal bacteria and has an important role in limiting the expansion T helper cells in the colon.

Fri Nov 01 00:00:00 EDT 2013

Nrf2 is essential for cholesterol crystal-induced inflammasome activation and exacerbation of atherosclerosis.

European journal of immunology

Freigang S,Ampenberger F,Spohn G,Heer S,Shamshiev AT,Kisielow J,Hersberger M,Yamamoto M,Bachmann MF,Kopf M

13-7123 was used in an ELISA assay to identify NF-E2-related 2 as an essential positive regulator of inflammasome activation and IL-1-mediated vascular inflammation.

Fri Jul 01 00:00:00 EDT 2011

IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE).

PloS one

Rynda A,Maddaloni M,Ochoa-Repáraz J,Callis G,Pascual DW

13-7123 was used in an ELISA assay to demonstrate the potential for IL-28 as an alternative therapy for EAE.

Thu Jan 14 00:00:00 EST 2010

Macrophage-mediated but gamma interferon-independent innate immune responses control the primary wave of Plasmodium yoelii parasitemia.

Infection and immunity

Couper KN,Blount DG,Hafalla JC,van Rooijen N,de Souza JB,Riley EM

13-7123 was used in an ELISA assay to discern the factors that control the primary wave of plasmodium yoelii parasitaemia.

Sat Dec 01 00:00:00 EST 2007

Host cell Ca2+ and protein kinase C regulate innate recognition of Toxoplasma gondii.

Journal of cell science

Masek KS,Fiore J,Leitges M,Yan SF,Freedman BD,Hunter CA

13-7123 was used in an ELISA assay to investigate the role of Ca2+ in Toxoplasma gondii infection.

Wed Nov 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CLMF p35; CLMF p40; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; IL-12 subunit p35; IL-12 subunit p40; IL-12A; IL-12B; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12 p40; IL23; il23; IL23 p41; ILN; Interleukin; Interleukin 12; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; Interleukin12; RP23-388G23.1

View more View less

Gene Aliases: Il-12a; Il-12b; IL-12p35; Il-12p40; IL-23; Il12a; Il12b; Il12p40; Il23a; Ll12a; p19; p35; p40

View more View less

UniProt ID: (Mouse) P43431, (Mouse) P43432, (Mouse) Q9EQ14

View more View less

Entrez Gene ID: (Mouse) 16159, (Mouse) 16160, (Mouse) 83430

View more View less

Function(s)

cytokine activity protein binding interleukin-23 receptor binding cytokine interleukin superfamily intercellular signal molecule transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

IL-12/IL-23 p40 Monoclonal Antibody (C17.8), Biotin, eBioscience™

IL-12/IL-23 p40 Antibody (13-7123-81) in Flow

Product Details

13-7123-81

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!