Invitrogen

IL-12/IL-23 p40 Monoclonal Antibody (C17.8), Biotin, eBioscience™

IL-12/IL-23 p40 Antibody in Flow Cytometry (Flow)

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IL-12/IL-23 p40 Antibody (13-7123-81) in Flow

Nature communications 2018 - Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 13-7123-81) in Flow Cytometry

IL-12/IL-23 p40 Antibody in Neutralization (Neu)

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Product Details

13-7123-81

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

View 3 publications 3 publications

ELISA (ELISA)

0.5-2 µg/mL

View 9 publications 9 publications

Neutralization (Neu)

View 1 publication 1 publication

Functional Assay (Functional)

- -

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), Biotin, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

C17.8

Conjugate

Biotin Biotin Biotin

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_466928

Product Specific Information

Description: The C17.8 antibody reacts with the p40 subunit of IL-12 and IL-23. The C17.8 antibody has been found to neutralize IL-12 and IL-23 bioactivity. For specific neutralization of IL-23 only, please see clone G23-8 (anti-mouse IL-23 p19). IL-12 is a 70 kDa heterodimeric cytokine composed of two covalently linked chains, p40 and p35, and is secreted by activated monocytes, macrophages, and dendritic cells. IL-23 is a heterodimer composed of the disulfide-linked p19 protein and p40 protein, expressed by activated dendritic cells.

Applications Reported: The C17.8 antibody has been reported for use in IP, Western, ELISA, neutralization, and intracellular staining for flow cytometric analysis.

Applications Tested: The biotinylated C17.8 antibody has been tested as the detection antibody in three different sandwich ELISAs, for measurement of mouse IL-12 p70, mouse IL-23, or mouse IL-12 p40 total.

For mouse IL-12 p70 ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in sandwich ELISA for analysis of mouse IL-12 p70, in combination with the purified C18.2 antibody (14-7122) for capture and recombinant mouse IL-12 p70 (39-8121) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2,000 pg/mL - 15 pg/mL should be included in each ELISA plate. Super AquaBlue or ABTS are suitable substrates.

For mouse IL-23 ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse IL-23 protein levels, in combination with the purified G23-8 antibody (14-7232) for capture and recombinant mouse IL-23 (39-8231) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.25 - 1 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 4,000 pg/mL - 30 pg/mL should be included in each ELISA plate. Note: TMB (00-4201), rather than ABTS, should be used as the substrate.

For mouse IL-12 p40 Total ELISA: The biotinylated C17.8 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse total p40 (e.g., p40 monomer, p40 dimer, p40/p35, and p40/p19), in combination with the purified C15.6 antibody (14-7125) for capture and recombinant mouse IL-12 p40 (39-8122), IL-12 p70 (39-8121) or mouse IL-23 (39-8231) as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2,000 pg/mL - 15 pg/mL should be included in each ELISA plate. Super AquaBlue or ABTS are suitable substrates.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

IL-12 is an interleukin produced by dendritic cells, neutrophils, macrophages and human lymphoblastoid cells as a result of antigenic stimulation. IL-12 is a heterodimeric cytokine encoded by IL-12A (p35) and IL-12B (p40). IL-23 is a heterodimeric cytokine that shares a subunit with IL-12 (IL-12B) along with an IL-23A (IL-23p19) subunit. Functionally, IL-12 increases the expression of vascular endothelial adhesion molecule-1 on the endothelium, directs leukocytes towards tumors and facilitates the ischemic-hemorrhagic necrosis of the tumor tissue. In comparison, IL-23 plays an important role in stimulating memory T-cells, and is required for the induction, expansion, maintenance and downstream effector functions of Th17 cells, which play a vital role in upregulating neutrophil chemokines and various pro-inflammatory cytokines.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

Product References

Requirements for the differentiation of innate T-bet^high memory-phenotype CD4⁺ T lymphocytes under steady state.

Nature communications

Kawabe T,Yi J,Kawajiri A,Hilligan K,Fang D,Ishii N,Yamane H,Zhu J,Jankovic D,Kim KS,Trinchieri G,Sher A

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 13-7123-81) in Flow Cytometry

Mon Jul 06 00:00:00 EDT 2020

Microbiota Sensing by Mincle-Syk Axis in Dendritic Cells Regulates Interleukin-17 and -22 Production and Promotes Intestinal Barrier Integrity.

Immunity

Martínez-López M,Iborra S,Conde-Garrosa R,Mastrangelo A,Danne C,Mann ER,Reid DM,Gaboriau-Routhiau V,Chaparro M,Lorenzo MP,Minnerup L,Saz-Leal P,Slack E,Kemp B,Gisbert JP,Dzionek A,Robinson MJ,Rupérez FJ,Cerf-Bensussan N,Brown GD,Bernardo D,LeibundGut-Landmann S,Sancho D

13-7123-81 was used in ELISA to conclude that sensing of commensals by Mincle and Syk signaling in CD11c+ cells reinforces intestinal immune barrier and promotes host-microbiota mutualism, preventing systemic inflammation.

Tue Feb 19 00:00:00 EST 2019

Absence of GdX/UBL4A Protects against Inflammatory Diseases by Regulating NF-кB Signaling in Macrophages and Dendritic Cells.

Theranostics

Liu C,Zhou Y,Li M,Wang Y,Yang L,Yang S,Feng Y,Wang Y,Wang Y,Ren F,Li J,Dong Z,Chin YE,Fu X,Wu L,Chang Z

13-7123 was used in an ELISA assay to investigate whether ubiquitin-like protein 4A has a role during the innate immune response.

Fri Jan 31 00:00:00 EST 2020

p38α has an important role in antigen cross-presentation by dendritic cells.

Cellular & molecular immunology

Zhou Y,Wu J,Liu C,Guo X,Zhu X,Yao Y,Jiao Y,He P,Han J,Wu L

13-7123 was used in an ELISA assay to study whether the p38 signalling pathway affects the important antigen presentation function of dendritic cells.

Thu Mar 01 00:00:00 EST 2018

Chloroquine modulates antitumor immune response by resetting tumor-associated macrophages toward M1 phenotype.

Nature communications

Chen D,Xie J,Fiskesund R,Dong W,Liang X,Lv J,Jin X,Liu J,Mo S,Zhang T,Cheng F,Zhou Y,Zhang H,Tang K,Ma J,Liu Y,Huang B

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 13-7123-81) in Flow Cytometry

Wed Feb 28 00:00:00 EST 2018

Analysis of the Trichuris suis excretory/secretory proteins as a function of life cycle stage and their immunomodulatory properties.

Scientific reports

Leroux LP,Nasr M,Valanparambil R,Tam M,Rosa BA,Siciliani E,Hill DE,Zarlenga DS,Jaramillo M,Weinstock JV,Geary TG,Stevenson MM,Urban JF,Mitreva M,Jardim A

13-7123 was used in an ELISA assay to analyse the Trichuris suis excretory/secretory proteins as a function of life cycle stage and their immunomodulatory properties.

Mon Oct 29 00:00:00 EDT 2018

CD1b-autoreactive T cells recognize phospholipid antigens and contribute to antitumor immunity against a CD1b⁺ T cell lymphoma.

Oncoimmunology

Bagchi S,Li S,Wang CR

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 13-7123-81) in Neutralization

Tue Jan 12 00:00:00 EST 2021

CX(3)CR1(+) macrophages support IL-22 production by innate lymphoid cells during infection with Citrobacter rodentium.

Mucosal immunology

Manta C,Heupel E,Radulovic K,Rossini V,Garbi N,Riedel CU,Niess JH

Published figure using IL-12/IL-23 p40 monoclonal antibody (Product # 13-7123-81) in Flow Cytometry

Tue Jan 01 00:00:00 EST 2013

Intestinal bacteria induce TSLP to promote mutualistic T-cell responses.

Mucosal immunology

Mosconi I,Geuking MB,Zaiss MM,Massacand JC,Aschwanden C,Kwong Chung CK,McCoy KD,Harris NL

13-7123 was used in an ELISA assay to show that steady-state thymic stromal lymphopoietin expression requires intestinal bacteria and has an important role in limiting the expansion T helper cells in the colon.

Fri Nov 01 00:00:00 EDT 2013

Nrf2 is essential for cholesterol crystal-induced inflammasome activation and exacerbation of atherosclerosis.

European journal of immunology

Freigang S,Ampenberger F,Spohn G,Heer S,Shamshiev AT,Kisielow J,Hersberger M,Yamamoto M,Bachmann MF,Kopf M

13-7123 was used in an ELISA assay to identify NF-E2-related 2 as an essential positive regulator of inflammasome activation and IL-1-mediated vascular inflammation.

Fri Jul 01 00:00:00 EDT 2011

IL-28 supplants requirement for T(reg) cells in protein sigma1-mediated protection against murine experimental autoimmune encephalomyelitis (EAE).

PloS one

Rynda A,Maddaloni M,Ochoa-Repáraz J,Callis G,Pascual DW

13-7123 was used in an ELISA assay to demonstrate the potential for IL-28 as an alternative therapy for EAE.

Thu Jan 14 00:00:00 EST 2010

Macrophage-mediated but gamma interferon-independent innate immune responses control the primary wave of Plasmodium yoelii parasitemia.

Infection and immunity

Couper KN,Blount DG,Hafalla JC,van Rooijen N,de Souza JB,Riley EM

13-7123 was used in an ELISA assay to discern the factors that control the primary wave of plasmodium yoelii parasitaemia.

Sat Dec 01 00:00:00 EST 2007

Host cell Ca2+ and protein kinase C regulate innate recognition of Toxoplasma gondii.

Journal of cell science

Masek KS,Fiore J,Leitges M,Yan SF,Freedman BD,Hunter CA

13-7123 was used in an ELISA assay to investigate the role of Ca2+ in Toxoplasma gondii infection.

Wed Nov 01 00:00:00 EST 2006

Bioinformatics

Protein Aliases: CLMF p35; CLMF p40; Cytotoxic lymphocyte maturation factor 35 kDa subunit; Cytotoxic lymphocyte maturation factor 40 kDa subunit; il 12; IL 12/IL 23p40; il 23; IL 23 p40; IL-12 subunit p35; IL-12 subunit p40; IL-12A; IL-12B; IL-23 subunit alpha; IL-23-A; IL-23p19; IL12 p40; IL23; il23; IL23 p41; ILN; Interleukin; Interleukin 12; Interleukin-12 subunit alpha; Interleukin-12 subunit beta; Interleukin-23 subunit alpha; Interleukin-23 subunit p19; Interleukin12; RP23-388G23.1

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Gene Aliases: Il-12a; Il-12b; IL-12p35; Il-12p40; IL-23; Il12a; Il12b; Il12p40; Il23a; Ll12a; p19; p35; p40

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UniProt ID: (Mouse) P43431, (Mouse) P43432, (Mouse) Q9EQ14

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Entrez Gene ID: (Mouse) 16159, (Mouse) 16160, (Mouse) 83430

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Function(s)

cytokine activity protein binding interleukin-23 receptor binding cytokine interleukin superfamily intercellular signal molecule transmembrane signal receptor

Process(es)

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IL-12/IL-23 p40 Monoclonal Antibody (C17.8), Biotin, eBioscience™

IL-12/IL-23 p40 Antibody (13-7123-81) in Flow

Product Details

13-7123-81

Flow Cytometry (Flow)

ELISA (ELISA)

Neutralization (Neu)

Functional Assay (Functional)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

Bioinformatics

Performance Guarantee

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