Invitrogen

IgM Monoclonal Antibody (eB121-15F9), eFluor™ 450, eBioscience™

7 Published Figures

12 References

View all (30) Mouse IgM antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (7)

FIGURE: 1 / 8

IgM Antibody (48-5890-82) in Flow

Staining of C57Bl/6 bone marrow cells with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.25 µg of Rat IgG2a kappa Isotype Control eFluor® 450 (Product # 48-4321-82) (left) or 0.25 µg of Anti-Mouse IgM eFluor® 450 (right). Total viable cells were used for analysis.

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Product Details

48-5890-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.5 µg/test

View 12 publications 12 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Rat / IgG2a, kappa

Recommended Isotype Control

Rat IgG2a kappa Isotype Control (eBR2a), eFluor™ 450, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eB121-15F9

Conjugate

eFluor™ 450 eFluor™ 450 eFluor™ 450

Excitation/Emission Max

405/445 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10671539

Product Specific Information

Description: The eB121-15F9 monoclonal antibody reacts with the mu heavy chain of mouse IgM. It does not react with other classes of mouse immunoglobulin including IgD, IgG or IgA. IgM is expressed intracellularly, during early stages of B lymphopoiesis, and then on the surface of more mature B cells in the bone marrow and peripheral B cells. Fluorochrome conjugated eB121-15F9 can be used as a detection secondary for mouse IgM.

Applications Reported: This eB121-15F9 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This eB121-15F9 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.

Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.

Filtration: 0.2 µm post-manufacturing filtered.

Target Information

Mouse IgM is an immunoglobulin M antibody that is produced by B cells in response to an antigen. It is the first antibody produced during an immune response and is the largest immunoglobulin in the serum. Mouse IgM is composed of five heavy chains and five light chains, and it is involved in complement activation and agglutination of antigens. It plays a crucial role in the innate immune response and is important for neutralizing pathogens before the adaptive immune response is fully activated. Mouse IgM is widely used in research and diagnostic applications, including ELISA, Western blotting, and immunohistochemistry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

NDRG1 is induced by antigen-receptor signaling but dispensable for B and T cell self-tolerance.

Communications biology

Hodgson R,Xu X,Anzilotti C,Deobagkar-Lele M,Crockford TL,Kepple JD,Cawthorne E,Bhandari A,Cebrian-Serrano A,Wilcock MJ,Davies B,Cornall RJ,Bull KR

48-5890-82 was used in Flow cytometry/Cell sorting to find that B cell tolerance is maintained, and NDRG1 does not play a role in downstream responses during re-stimulation of in vivo antigen-experienced CD4+ T cells, demonstrating that NDGR1 is functionally redundant for lymphocyte anergy.

Thu Nov 10 00:00:00 EST 2022

CPHEN-011: Comprehensive phenotyping of murine lung resident lymphocytes after recovery from pneumococcal pneumonia.

Cytometry. Part A : the journal of the International Society for Analytical Cytology

Shenoy AT,Lyon De Ana C,Barker KA,Arafa EI,Martin IMC,Mizgerd JP,Belkina AC

48-5890-82 was used in Flow Cytometry to identify unforeseen heterogeneity in lung resident adaptive immune landscape which includes unexpected subsets of TRM and BRM cells.

Tue Nov 01 00:00:00 EDT 2022

Reciprocal regulation of RIG-I and XRCC4 connects DNA repair with RIG-I immune signaling.

Nature communications

Guo G,Gao M,Gao X,Zhu B,Huang J,Tu X,Kim W,Zhao F,Zhou Q,Zhu S,Wu Z,Yan Y,Zhang Y,Zeng X,Zhu Q,Yin P,Luo K,Sun J,Deng M,Lou Z

Published figure using IgM monoclonal antibody (Product # 48-5890-82) in Flow Cytometry

Mon Apr 12 00:00:00 EDT 2021

Mutations in topoisomerase IIβ result in a B cell immunodeficiency.

Nature communications

Broderick L,Yost S,Li D,McGeough MD,Booshehri LM,Guaderrama M,Brydges SD,Kucharova K,Patel NC,Harr M,Hakonarson H,Zackai E,Cowell IG,Austin CA,Hügle B,Gebauer C,Zhang J,Xu X,Wang J,Croker BA,Frazer KA,Putnam CD,Hoffman HM

48-5890-82 was used in Flow Cytometry to identify mutations in type II topoisomerase as a driver of defective proliferation and survival of B2 cells, leading to a B cell immunodeficiency.

Tue Aug 13 00:00:00 EDT 2019

Indoleamine 2,3-Dioxygenase 1, Increased in Human Gastric Pre-Neoplasia, Promotes Inflammation and Metaplasia in Mice and Is Associated With Type II Hypersensitivity/Autoimmunity.

Gastroenterology

El-Zaatari M,Bass AJ,Bowlby R,Zhang M,Syu LJ,Yang Y,Grasberger H,Shreiner A,Tan B,Bishu S,Leung WK,Todisco A,Kamada N,Cascalho M,Dlugosz AA,Kao JY

Published figure using IgM monoclonal antibody (Product # 48-5890-82) in Flow Cytometry

Mon Jan 01 00:00:00 EST 2018

Peli1 negatively regulates noncanonical NF-κB signaling to restrain systemic lupus erythematosus.

Nature communications

Liu J,Huang X,Hao S,Wang Y,Liu M,Xu J,Zhang X,Yu T,Gan S,Dai D,Luo X,Lu Q,Mao C,Zhang Y,Shen N,Li B,Huang M,Zhu X,Jin J,Cheng X,Sun SC,Xiao Y

48-5890-82 was used in Flow Cytometry to establish Peli1 as a negative regulator of the noncanonical NF-κB pathway in the context of restraining the pathogenesis of lupus-like disease.

Mon Mar 19 00:00:00 EDT 2018

Eosinophils are required to suppress Th2 responses in Peyer's patches during intestinal infection by nematodes.

Mucosal immunology

Strandmark J,Steinfelder S,Berek C,Kühl AA,Rausch S,Hartmann S

48-5890 was used in Flow cytometry/Cell sorting to study whether eosinophils affect the local T-cell and antibody response in the gut-associated lymphoid tissue during enteric infections.

Mon May 01 00:00:00 EDT 2017

lncRNA requirements for mouse acute myeloid leukemia and normal differentiation.

eLife

Delás MJ,Sabin LR,Dolzhenko E,Knott SR,Munera Maravilla E,Jackson BT,Wild SA,Kovacevic T,Stork EM,Zhou M,Erard N,Lee E,Kelley DR,Roth M,Barbosa IA,Zuber J,Rinn JL,Smith AD,Hannon GJ

48-5890 was used in Flow cytometry/Cell sorting to assess lncRNA function via an in vivo RNAi screen in a model of acute myeloid leukemia.

Wed Sep 06 00:00:00 EDT 2017

Survival of Igα-Deficient Mature B Cells Requires BAFF-R Function.

Journal of immunology (Baltimore, Md. : 1950)

Levit-Zerdoun E,Becker M,Pohlmeyer R,Wilhelm I,Maity PC,Rajewsky K,Reth M,Hobeika E

48-5890 was used in Flow cytometry/Cell sorting to assess the importance of the expression of a functional B-cell receptor for B cell maintenance.

Tue Mar 01 00:00:00 EST 2016

NFATc1 supports imiquimod-induced skin inflammation by suppressing IL-10 synthesis in B cells.

Nature communications

Alrefai H,Muhammad K,Rudolf R,Pham DA,Klein-Hessling S,Patra AK,Avots A,Bukur V,Sahin U,Tenzer S,Goebeler M,Kerstan A,Serfling E

Published figure using IgM monoclonal antibody (Product # 48-5890-82) in Flow Cytometry

Wed May 25 00:00:00 EDT 2016

PP4 is essential for germinal center formation and class switch recombination in mice.

PloS one

Chen MY,Chen YP,Wu MS,Yu GY,Lin WJ,Tan TH,Su YW

48-5890 was used in Flow cytometry/Cell sorting to elucidate the role of the phosphatase PP4 in mature B cells.

Tue Dec 15 00:00:00 EST 2015

The histone methyltransferase MMSET regulates class switch recombination.

Journal of immunology (Baltimore, Md. : 1950)

Pei H,Wu X,Liu T,Yu K,Jelinek DF,Lou Z

Published figure using IgM monoclonal antibody (Product # 48-5890-82) in Flow Cytometry

Tue Jan 15 00:00:00 EST 2013

Bioinformatics

Gene Aliases: AI326478; Igh-6; Igh-M; Igh6; Igm; muH

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Entrez Gene ID: (Mouse) 16019

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Function(s)

immunoglobulin

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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IgM Monoclonal Antibody (eB121-15F9), eFluor™ 450, eBioscience™

IgM Antibody (48-5890-82) in Flow

Product Details

48-5890-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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