Invitrogen

MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), APC-eFluor™ 780, eBioscience™

17 Published Figures

18 References

View all (18) MHC Class I (H-2Kb) antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (17)

MHC Class I (H-2Kb) Antibody in Flow Cytometry (Flow)

FIGURE: 1 / 18

MHC Class I (H-2Kb) Antibody (47-5958-82) in Flow

BALB/c (left) or C57BL/6 (right) mouse splenocytes were stained with 0.125 µg of Mouse IgG2a kappa Isotype Control, APC-eFluor 780 (Product # 47-4724-81) (blue histogram) or 0.125 µg of MHC Class I (H-2Kb) Monoclonal Antibody, APC-eFluor 780 (purple histogram). Cells in the lymphocyte gate were used for analysis.

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Product Details

47-5958-82

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

0.25 µg/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Mouse

Published species

Mouse

Host/Isotype

Mouse / IgG2a, kappa

Recommended Isotype Control

Mouse IgG2a kappa Isotype Control (eBM2a), APC-eFluor™ 780, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

AF6-88.5.5.3

Conjugate

APC-eFluor™ 780 APC-eFluor™ 780 APC-eFluor™ 780

Excitation/Emission Max

756/785 nm View spectra

Form

Liquid

Concentration

0.2 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2815170

Product Specific Information

Description: This AF6-88.5.5.3 monoclonal antibody reacts with the H-2Kb MHC class I alloantigen. H-2Kb is involved in antigen presentation to T cells expressing CD3/TCR and CD8. Reactivity to other haplotypes (e.g. d, f, j, k, p, q, r, s, u, and v) has not been observed.

Applications Reported: This AF6-88.5.5.3 antibody has been reported for use in flow cytometric analysis.

Applications Tested: This AF6-88.5.5.3 antibody has been tested by flow cytometric analysis of mouse splenocytes. This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633-647 nm). Please make sure that your instrument is capable of detecting this fluorochrome.

Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.

Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222-49) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333-57) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.

Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.

Target Information

This protein is an MHC class I alloantigen, which is involved in antigen presentation to T cells expressing CD3/TCR and CD8.

HLA and MHC antibodies play a significant role in Immunopeptidomics, facilitating the identification and characterization of neoantigens through high-performance liquid chromatography coupled to tandem Mass Spectrometry.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

High-Dose Acetaminophen with N-acetylcysteine Rescue Inhibits M2 Polarization of Tumor-Associated Macrophages.

Cancers

Bryan A,Pingali P,Joslyn M,Li H,Bernas T,Koblinski J,Landry J,Lee WS,Patel B,Neuwelt A

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Thu Sep 28 00:00:00 EDT 2023

CD8α+ dendritic cells potentiate antitumor and immune activities against murine ovarian cancers.

Scientific reports

Lee SW,Lee H,Lee KW,Kim MJ,Kang SW,Lee YJ,Kim H,Kim YM

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Tue Jan 03 00:00:00 EST 2023

Senescence Rewires Microenvironment Sensing to Facilitate Antitumor Immunity.

Cancer discovery

Chen HA,Ho YJ,Mezzadra R,Adrover JM,Smolkin R,Zhu C,Woess K,Bernstein N,Schmitt G,Fong L,Luan W,Wuest A,Tian S,Li X,Broderick C,Hendrickson RC,Egeblad M,Chen Z,Alonso-Curbelo D,Lowe SW

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Mon Feb 06 00:00:00 EST 2023

Genetic deletion of HVEM in a leukemia B cell line promotes a preferential increase of PD-1^- stem cell-like T cells over PD-1⁺ T cells curbing tumor progression.

Frontiers in immunology

Del Rio ML,de Juan CY,Roncador G,Caleiras E,Álvarez-Esteban R,Pérez-Simón JA,Rodriguez-Barbosa JI

47-5958-82 was used in Flow cytometry/Cell sorting to highlight the role of co-inhibitory signals delivered by Herpes Virus Entry Mediator (HVEM, TNFRSF14) upon engagement of BTLA on T cells and NK cells, placing HVEM/BTLA interaction in the spotlight as a novel immune checkpoint for the reinforcement of the anti-tumor responses in malignancies of hematopoietic origin.

Thu Aug 10 00:00:00 EDT 2023

Specific cannabinoids revive adaptive immunity by reversing immune evasion mechanisms in metastatic tumours.

Frontiers in immunology

Dada S,Ellis SLS,Wood C,Nohara LL,Dreier C,Garcia NH,Saranchova I,Munro L,Pfeifer CG,Eyford BA,Kari S,Garrovillas E,Caspani G,Al Haddad E,Gray PW,Morova T,Lack NA,Andersen RJ,Tjoelker L,Jefferies WA

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Mon Mar 20 00:00:00 EDT 2023

Mesenchymal stem cell exosome-derived miR-223 alleviates acute graft-versus-host disease via reducing the migration of donor T cells.

Stem cell research & therapy

Liu W,Zhou N,Liu Y,Zhang W,Li X,Wang Y,Zheng R,Zhang Y

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Fri Feb 26 00:00:00 EST 2021

Intravenous injection of the oncolytic virus M1 awakens antitumor T cells and overcomes resistance to checkpoint blockade.

Cell death & disease

Liu Y,Cai J,Liu W,Lin Y,Guo L,Liu X,Qin Z,Xu C,Zhang Y,Su X,Deng K,Yan G,Liang J

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Sat Dec 12 00:00:00 EST 2020

Bendamustine with total body irradiation conditioning yields tolerant T-cells while preserving T-cell-dependent graft-versus-leukemia.

Oncoimmunology

Stokes J,Hoffman EA,Molina MS,Kummet N,Simpson RJ,Zeng Y,Katsanis E

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Wed Jul 14 00:00:00 EDT 2021

CXCL14 suppresses human papillomavirus-associated head and neck cancer through antigen-specific CD8⁺ T-cell responses by upregulating MHC-I expression.

Oncogene

Westrich JA,Vermeer DW,Silva A,Bonney S,Berger JN,Cicchini L,Greer RO,Song JI,Raben D,Slansky JE,Lee JH,Spanos WC,Pyeon D

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Fri Nov 01 00:00:00 EDT 2019

CD1d highly expressed on DCs reduces lung tumor burden by enhancing antitumor immunity.

Oncology reports

Li Y,Zhao C,Liu J,Lu Z,Lu M,Gu J,Liu R

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Wed May 01 00:00:00 EDT 2019

Tumor-intrinsic response to IFNγ shapes the tumor microenvironment and anti-PD-1 response in NSCLC.

Life science alliance

Bullock BL,Kimball AK,Poczobutt JM,Neuwelt AJ,Li HY,Johnson AM,Kwak JW,Kleczko EK,Kaspar RE,Wagner EK,Hopp K,Schenk EL,Weiser-Evans MC,Clambey ET,Nemenoff RA

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Sat Jun 01 00:00:00 EDT 2019

Platelet-derived β2m regulates age related monocyte/macrophage functions.

Aging

Hilt ZT,Ture SK,Mohan A,Arne A,Morrell CN

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Wed Dec 18 00:00:00 EST 2019

Critical role for Sec22b-dependent antigen cross-presentation in antitumor immunity.

The Journal of experimental medicine

Alloatti A,Rookhuizen DC,Joannas L,Carpier JM,Iborra S,Magalhaes JG,Yatim N,Kozik P,Sancho D,Albert ML,Amigorena S

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Mon Aug 07 00:00:00 EDT 2017

Sec61 blockade by mycolactone inhibits antigen cross-presentation independently of endosome-to-cytosol export.

Proceedings of the National Academy of Sciences of the United States of America

Grotzke JE,Kozik P,Morel JD,Impens F,Pietrosemoli N,Cresswell P,Amigorena S,Demangel C

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Tue Jul 18 00:00:00 EDT 2017

Deletion of Wiskott-Aldrich syndrome protein triggers Rac2 activity and increased cross-presentation by dendritic cells.

Nature communications

Baptista MA,Keszei M,Oliveira M,Sunahara KK,Andersson J,Dahlberg CI,Worth AJ,Liedén A,Kuo IC,Wallin RP,Snapper SB,Eidsmo L,Scheynius A,Karlsson MC,Bouma G,Burns SO,Forsell MN,Thrasher AJ,Nylén S,Westerberg LS

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Mon Jul 18 00:00:00 EDT 2016

Favorable alteration of tumor microenvironment by immunomodulatory cytokines for efficient T-cell therapy in solid tumors.

PloS one

Tähtinen S,Kaikkonen S,Merisalo-Soikkeli M,Grönberg-Vähä-Koskela S,Kanerva A,Parviainen S,Vähä-Koskela M,Hemminki A

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Thu Apr 28 00:00:00 EDT 2016

Anti-angiogenic efficacy of 5'-triphosphate siRNA combining VEGF silencing and RIG-I activation in NSCLCs.

Oncotarget

Yuan D,Xia M,Meng G,Xu C,Song Y,Wei J

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Tue Oct 06 00:00:00 EDT 2015

Oxidized lipids block antigen cross-presentation by dendritic cells in cancer.

Journal of immunology (Baltimore, Md. : 1950)

Ramakrishnan R,Tyurin VA,Veglia F,Condamine T,Amoscato A,Mohammadyani D,Johnson JJ,Zhang LM,Klein-Seetharaman J,Celis E,Kagan VE,Gabrilovich DI

Published figure using MHC Class I (H-2Kb) monoclonal antibody (Product # 47-5958-82) in Flow Cytometry

Sat Mar 15 00:00:00 EDT 2014

Bioinformatics

Protein Aliases: class I major histocompatibility antigen H-2Kd; H-2 class I histocompatibility antigen, K-B alpha chain; H-2 class I histocompatibility antigen, K-D alpha chain; H-2 class I histocompatibility antigen, K-K alpha chain; H-2 class I histocompatibility antigen, K-Q alpha chain; H-2 class I histocompatibility antigen, K-W28 alpha chain; H-2K(B); H-2K(K); H-2K(Q); MHC class I antigen; MHC class I H2-K-b-alpha-2 cell surface glycoprotein; MHC class I heavy chain H2-K; MHC H2-K transplantation antigen

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Gene Aliases: H-2K; H-2K(d); H2-K; H2-K1; K-f

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UniProt ID: (Mouse) P01901

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Entrez Gene ID: (Mouse) 14972

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Function(s)

receptor binding protein binding beta-2-microglobulin binding peptide binding peptide antigen binding T cell receptor binding poly(A) RNA binding TAP binding protein heterodimerization activity major histocompatibility complex protein

Process(es)

positive regulation of T cell mediated cytotoxicity immune system process antigen processing and presentation of peptide antigen via MHC class I antigen processing and presentation of endogenous peptide antigen via MHC class I via ER pathway, TAP-dependent immune response antigen processing and presentation antigen processing and presentation of exogenous peptide antigen via MHC class I defense response to bacterium inner ear development

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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MHC Class I (H-2Kb) Monoclonal Antibody (AF6-88.5.5.3), APC-eFluor™ 780, eBioscience™

MHC Class I (H-2Kb) Antibody (47-5958-82) in Flow

Product Details

47-5958-82

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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