Invitrogen

CD14 Monoclonal Antibody (TuK4), FITC

2 Published Figures

14 References

View all (206) CD14 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 3

CD14 Antibody (MHCD1401) in Flow

Human peripheral blood monocytes stained using FITC of anti-human CD14 monoclonal antibody (clone Tuk4). The negative control profiles represent unstained cells.

Product Image Gallery

Product Details

MHCD1401

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-Dependent

View 8 publications 8 publications

Miscellaneous PubMed (Misc)

View 4 publications 4 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey, Sheep

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

TuK4

Immunogen

Human CD14

Conjugate

FITC FITC FITC

Excitation/Emission Max

498/517 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373108

Product Specific Information

Commonly used, FITC conjugates provide relatively high absorptivity, excellent fluorescence quantum yield, and good water solubility.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Development of a skin- and neuro-attenuated live vaccine for varicella.

Nature communications

Wang W,Pan D,Fu W,Ye X,Han J,Yang L,Jia J,Liu J,Zhu R,Zhang Y,Liu C,Ye J,Selariu A,Que Y,Zhao Q,Wu T,Li Y,Zhang J,Cheng T,Zhu H,Xia N

MHCD1401 was used in Flow Cytometry to design a live-attenuated varicella vaccine candidate, v7D.

Fri Feb 11 00:00:00 EST 2022

Development of a skin- and neuro-attenuated live vaccine for varicella.

Nature communications

Wang W,Pan D,Fu W,Ye X,Han J,Yang L,Jia J,Liu J,Zhu R,Zhang Y,Liu C,Ye J,Selariu A,Que Y,Zhao Q,Wu T,Li Y,Zhang J,Cheng T,Zhu H,Xia N

MHCD1401 was used in Flow Cytometry to design a live-attenuated varicella vaccine candidate, v7D.

Fri Feb 11 00:00:00 EST 2022

Mesenchymal stem cells negatively regulate CD4⁺ T cell activation in patients with primary Sjögren syndrome through the miRNA‑125b and miRNA‑155 TCR pathway.

Molecular medicine reports

Gong B,Zheng L,Lu Z,Huang J,Pu J,Pan S,Zhang M,Liu J,Tang J

MHCD1401 was used in Flow cytometry/Cell sorting to indicate that the expression levels of miRNA‚125b‚5p and miRNA‚155 in TCR‚activated CD4+ T cells from patients with pSS may provide insight regarding autoimmune diseases and offer a novel target for prospective treatment.

Fri Jan 01 00:00:00 EST 2021

Human Tumor-Associated Macrophage and Monocyte Transcriptional Landscapes Reveal Cancer-Specific Reprogramming, Biomarkers, and Therapeutic Targets.

Cancer cell

Cassetta L,Fragkogianni S,Sims AH,Swierczak A,Forrester LM,Zhang H,Soong DYH,Cotechini T,Anur P,Lin EY,Fidanza A,Lopez-Yrigoyen M,Millar MR,Urman A,Ai Z,Spellman PT,Hwang ES,Dixon JM,Wiechmann L,Coussens LM,Smith HO,Pollard JW

MHCD1401 was used in Flow cytometry/Cell sorting to show that monocyte subpopulation distribution and transcriptomes are significantly altered by the presence of endometrial and breast cancer.

Mon Apr 15 00:00:00 EDT 2019

Biomechanical Stability and Osteogenesis in a Tibial Bone Defect Treated by Autologous Ovine Cord Blood Cells-A Pilot Study.

Molecules (Basel, Switzerland)

Herten M,Zilkens C,Thorey F,Tassemeier T,Lensing-Höhn S,Fischer JC,Sager M,Krauspe R,Jäger M

MHCD1401 was used in Flow Cytometry to elucidate the impact of autologous umbilical cord blood cells on bone regeneration and biomechanical stability in an ovine tibial bone defect.

Tue Jan 15 00:00:00 EST 2019

Alterations in the properties of the cell membrane due to glycosphingolipid accumulation in a model of Gaucher disease.

Scientific reports

Batta G,Soltész L,Kovács T,Bozó T,Mészár Z,Kellermayer M,Szöllősi J,Nagy P

Tue Jan 09 00:00:00 EST 2018

CD14 and IL18 gene polymorphisms associated with colorectal cancer subsite risks among atomic bomb survivors.

Human genome variation

Hu Y,Yoshida K,Cologne JB,Maki M,Morishita Y,Sasaki K,Hayashi I,Ohishi W,Hida A,Kyoizumi S,Kusunoki Y,Tokunaga K,Nakachi K,Hayashi T

MHCD1401 was used in Immunofluorescence to suggest the involvement of a CD14-mediated inflammatory response and an IL18-mediated response among A-bomb survivors in distal colorectal cancer development.

Fri Apr 15 00:00:00 EDT 2016

CD14 and IL18 gene polymorphisms associated with colorectal cancer subsite risks among atomic bomb survivors.

Human genome variation

Hu Y,Yoshida K,Cologne JB,Maki M,Morishita Y,Sasaki K,Hayashi I,Ohishi W,Hida A,Kyoizumi S,Kusunoki Y,Tokunaga K,Nakachi K,Hayashi T

Fri Apr 15 00:00:00 EDT 2016

Regulation of osteoarthritis-associated key mediators by TNFα and IL-10: effects of IL-10 overexpression in human synovial fibroblasts and a synovial cell line.

Cell and tissue research

Mrosewski I,Jork N,Gorte K,Conrad C,Wiegand E,Kohl B,Ertel W,John T,Oberholzer A,Kaps C,Schulze-Tanzil G

MHCD1401 was used in immunocytochemistry to investigate the effect of TNF and IL-10 on synovial fibroblasts

Tue Jul 01 00:00:00 EDT 2014

Transmission of HIV-1 by primary human uterine epithelial cells and stromal fibroblasts.

The Journal of infectious diseases

Asin SN,Fanger MW,Wildt-Perinic D,Ware PL,Wira CR,Howell AL

MHCD1401 was used in Flow cytometry/Cell sorting to characterise the life-cycle events of human immunodeficiency virus type 1 in primary cultures of human uterine epithelial cells and stromal fibroblasts.

Thu Jul 15 00:00:00 EDT 2004

Susceptibility of immature and mature Langerhans cell-type dendritic cells to infection and immunomodulation by human cytomegalovirus.

Journal of virology

Hertel L,Lacaille VG,Strobl H,Mellins ED,Mocarski ES

MHCD1401 was used in flow cytometry to report that CD34(+) progenitor cell-derived Langerhans cells-type DCs exhibit a differentiation state-dependent susceptibility to CMV infection.

Tue Jul 01 00:00:00 EDT 2003

Flow cytometry in the bone marrow staging of mature B-cell neoplasms.

Cytometry. Part B, Clinical cytometry

Stacchini A,Demurtas A,Godio L,Martini G,Antinoro V,Palestro G

MHCD1401 was used in flow cytometry to ask if flow cytometry can be used for the detection of bone marrow involvement in the different types of B-cell malignant neoplasms.

Tue Jul 01 00:00:00 EDT 2003

Latent cytomegalovirus down-regulates major histocompatibility complex class II expression on myeloid progenitors.

Blood

Slobedman B,Mocarski ES,Arvin AM,Mellins ED,Abendroth A

MHCD1401 was used in Flow Cytometry to study the mechanism involving the modulation of major histocompatibility complex class I and II proteins.

Tue Oct 15 00:00:00 EDT 2002

Dependence of bacterial protein adhesins on toll-like receptors for proinflammatory cytokine induction.

Clinical and diagnostic laboratory immunology

Hajishengallis G,Martin M,Sojar HT,Sharma A,Schifferle RE,DeNardin E,Russell MW,Genco RJ

MHCD1401 was used in flow cytometry to determine of proinflammatory cytokine responses induced by certain bacterial protein adhesins are dependent on TLRs.

Fri Mar 01 00:00:00 EST 2002

Cytokine expression, natural killer cell activation, and phenotypic changes in lymphoid cells from rhesus macaques during acute infection with pathogenic simian immunodeficiency virus.

Journal of virology

Giavedoni LD,Velasquillo MC,Parodi LM,Hubbard GB,Hodara VL

MHCD1401 was used in flow cytometry to examine the innate and adaptive immune responses of rhesus macaques infected with SIVmac251.

Tue Feb 01 00:00:00 EST 2000

Bioinformatics

Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

View more View less

Gene Aliases: CD14

View more View less

UniProt ID: (Human) P08571

View more View less

Entrez Gene ID: (Human) 929

View more View less

Function(s)

lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding transmembrane signal receptor

Process(es)

MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway receptor-mediated endocytosis phagocytosis apoptotic process inflammatory response cell surface receptor signaling pathway I-kappaB kinase/NF-kappaB signaling response to heat lipopolysaccharide-mediated signaling pathway response to magnesium ion positive regulation of type I interferon production positive regulation of interferon-gamma production positive regulation of tumor necrosis factor production negative regulation of MyD88-independent toll-like receptor signaling pathway toll-like receptor 4 signaling pathway response to tumor necrosis factor TRIF-dependent toll-like receptor signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway innate immune response response to ethanol positive regulation of endocytosis positive regulation of cytokine secretion response to electrical stimulus necroptotic process cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD14 Monoclonal Antibody (TuK4), FITC

CD14 Antibody (MHCD1401) in Flow

Product Details

MHCD1401

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!