Invitrogen

CD14 Monoclonal Antibody (TuK4), Pacific Blue™

6 Published Figures

20 References

View all (206) CD14 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)
Published Figures (6)

FIGURE: 1 / 7

CD14 Antibody (MHCD1428) in Flow

Human peripheral blood monocytes stained using Pacific Blue Pacific Blue-H fluorochrome conjugates of anti-human CD14 monoclonal antibody (clone TuK4). The negative control profiles represent unstained cells.

Product Image Gallery

Product Details

MHCD1428

Applications

Tested Dilution

Publications

Immunocytochemistry (ICC/IF)

View 2 publications 2 publications

Flow Cytometry (Flow)

Assay-Dependent

View 15 publications 15 publications

Miscellaneous PubMed (Misc)

View 3 publications 3 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Rhesus monkey

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

TuK4

Immunogen

Human CD14

Conjugate

Pacific Blue™ Pacific Blue™ Pacific Blue™

Excitation/Emission Max

404/455 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373537

Product Specific Information

Our Pacific Blue™ dye is optimally excited by the violet laser and conjugates of this dye are strongly fluorescent even at neutral pH. Pacific Blue™ and Pacific Orange™ dye conjugates can be simultaneously excited at 405 nm and emit at 455 nm and 551 nm, respectively, facilitating two-color analysis.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

IFN-α with dasatinib broadens the immune repertoire in patients with chronic-phase chronic myeloid leukemia.

The Journal of clinical investigation

Huuhtanen J,Ilander M,Yadav B,Dufva OM,Lähteenmäki H,Kasanen T,Klievink J,Olsson-Strömberg U,Stentoft J,Richter J,Koskenvesa P,Höglund M,Söderlund S,Dreimane A,Porkka K,Gedde-Dahl T,Gjertsen BT,Stenke L,Myhr-Eriksson K,Markevärn B,Lübking A,Dimitrijevic A,Udby L,Bjerrum OW,Hjorth-Hansen H,Mustjoki S

MHCD1428 was used in Immuno-assay to support the combination of IFN-α with TKI therapy, as IFN-α broadens the immune repertoire and restores immunological function.

Thu Sep 01 00:00:00 EDT 2022

ASXL1 mutations are associated with distinct epigenomic alterations that lead to sensitivity to venetoclax and azacytidine.

Blood cancer journal

Rahmani NE,Ramachandra N,Sahu S,Gitego N,Lopez A,Pradhan K,Bhagat TD,Gordon-Mitchell S,Pena BR,Kazemi M,Rao K,Giricz O,Maqbool SB,Olea R,Zhao Y,Zhang J,Dolatshad H,Tittrea V,Tatwavedi D,Singh S,Lee J,Sun T,Steidl U,Shastri A,Inoue D,Abdel-Wahab O,Pellagatti A,Gavathiotis E,Boultwood J,Verma A

Published figure using CD14 monoclonal antibody (Product # MHCD1428) in Flow Cytometry

Tue Sep 21 00:00:00 EDT 2021

GAD-alum immunotherapy in type 1 diabetes expands bifunctional Th1/Th2 autoreactive CD4 T cells.

Diabetologia

Arif S,Gomez-Tourino I,Kamra Y,Pujol-Autonell I,Hanton E,Tree T,Melandri D,Hull C,Wherrett DK,Beam C,Roep BO,Lorenc A,Peakman M

MHCD1428 was used in Immunocytochemistry-immunoflourescence to show that GAD-alum immunisation activates and propagates GAD-specific CD4 T cells with a distinctive bifunctional phenotype, the functional analysis of which might be important in understanding therapeutic responses.

Mon Jun 01 00:00:00 EDT 2020

GAD-alum immunotherapy in type 1 diabetes expands bifunctional Th1/Th2 autoreactive CD4 T cells.

Diabetologia

Arif S,Gomez-Tourino I,Kamra Y,Pujol-Autonell I,Hanton E,Tree T,Melandri D,Hull C,Wherrett DK,Beam C,Roep BO,Lorenc A,Peakman M

Mon Jun 01 00:00:00 EDT 2020

Principles Governing Establishment versus Collapse of HIV-1 Cellular Spread.

Cell host & microbe

Hataye JM,Casazza JP,Best K,Liang CJ,Immonen TT,Ambrozak DR,Darko S,Henry AR,Laboune F,Maldarelli F,Douek DC,Hengartner NW,Yamamoto T,Keele BF,Perelson AS,Koup RA

MHCD1428 was used in Flow Cytometry to explore the mechanism of exponential viral spreading of HIV-1 between cells.

Wed Dec 11 00:00:00 EST 2019

Over-expression of PD-1 Does Not Predict Leukemic Relapse after Allogeneic Stem Cell Transplantation.

Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation

Jain P,Tian X,Cordes S,Chen J,Cantilena CR,Bradley C,Panjwani R,Chinian F,Keyvanfar K,Battiwalla M,Muranski P,Barrett AJ,Ito S

MHCD1428 was used in Flow cytometry/Cell sorting to analyse the relationship of leukaemia relapse with T-cell marker kinetics.

Fri Feb 01 00:00:00 EST 2019

Genetic Inactivation of CD33 in Hematopoietic Stem Cells to Enable CAR T Cell Immunotherapy for Acute Myeloid Leukemia.

Cell

Kim MY,Yu KR,Kenderian SS,Ruella M,Chen S,Shin TH,Aljanahi AA,Schreeder D,Klichinsky M,Shestova O,Kozlowski MS,Cummins KD,Shan X,Shestov M,Bagg A,Morrissette JJD,Sekhri P,Lazzarotto CR,Calvo KR,Kuhns DB,Donahue RE,Behbehani GK,Tsai SQ,Dunbar CE,Gill S

MHCD1428 was used in Flow cytometry/Cell sorting to illuminate a novel approach to antigen-specific immunotherapy by genetically engineering the host to avoid on-target, off-tumor toxicity.

Thu May 31 00:00:00 EDT 2018

Ten weeks of high-intensity interval walk training is associated with reduced disease activity and improved innate immune function in older adults with rheumatoid arthritis: a pilot study.

Arthritis research & therapy

Bartlett DB,Willis LH,Slentz CA,Hoselton A,Kelly L,Huebner JL,Kraus VB,Moss J,Muehlbauer MJ,Spielmann G,Kraus WE,Lord JM,Huffman KM

MHCD1428 was used in Flow cytometry/Cell sorting to determine whether 10 weeks of a walking-based HIIT program would be associated with health improvements as measured by disease activity and aerobic fitness.

Thu Jun 14 00:00:00 EDT 2018

Barcoding of Macaque Hematopoietic Stem and Progenitor Cells: A Robust Platform to Assess Vector Genotoxicity.

Molecular therapy. Methods & clinical development

Yabe IM,Truitt LL,Espinoza DA,Wu C,Koelle S,Panch S,Corat MAF,Winkler T,Yu KR,Hong SG,Bonifacino A,Krouse A,Metzger M,Donahue RE,Dunbar CE

MHCD1428 was used in Flow cytometry/Cell sorting to investigate the impact of different vector designs on the clonal behaviour of haematopoietic stem and progenitor cells during in vivo expansion.

Fri Dec 14 00:00:00 EST 2018

Effect of Antiretroviral Therapy on the Memory and Activation Profiles of B Cells in HIV-Infected African Women.

Journal of immunology (Baltimore, Md. : 1950)

Tanko RF,Soares AP,Müller TL,Garrett NJ,Samsunder N,Abdool Karim Q,Abdool Karim SS,Riou C,Burgers WA

MHCD1428 was used in Flow cytometry/Cell sorting to study the effect of antiretroviral therapy on B cells in HIV infection.

Wed Feb 01 00:00:00 EST 2017

T cell receptor β-chains display abnormal shortening and repertoire sharing in type 1 diabetes.

Nature communications

Gomez-Tourino I,Kamra Y,Baptista R,Lorenc A,Peakman M

MHCD1428 was used in Flow cytometry/Cell sorting to suggest that short CDR3s increase the potential for self-recognition, conferring heightened risk of autoimmune disease.

Mon Nov 27 00:00:00 EST 2017

Myocardial ischemia and reperfusion leads to transient CD8 immune deficiency and accelerated immunosenescence in CMV-seropositive patients.

Circulation research

Hoffmann J,Shmeleva EV,Boag SE,Fiser K,Bagnall A,Murali S,Dimmick I,Pircher H,Martin-Ruiz C,Egred M,Keavney B,von Zglinicki T,Das R,Todryk S,Spyridopoulos I

MHCD1428 was used in flow cytometry to study the incidence of coronary heart disease in cytomegalovirus-seropositive individuals.

Fri Jan 02 00:00:00 EST 2015

Immunization with Ty21a live oral typhoid vaccine elicits crossreactive multifunctional CD8+ T-cell responses against Salmonella enterica serovar Typhi, S. Paratyphi A, and S. Paratyphi B in humans.

Mucosal immunology

Wahid R,Fresnay S,Levine MM,Sztein MB

MHCD1428 was used in Immunocytochemistry-immunoflourescence to suggest that Ty21a elicits multifunctional CMI responses against Salmonella that could be critical in clearing the infection.

Sun Nov 01 00:00:00 EDT 2015

Atorvastatin induces T cell proliferation by a telomerase reverse transcriptase (TERT) mediated mechanism.

Atherosclerosis

Bennaceur K,Atwill M,Al Zhrany N,Hoffmann J,Keavney B,Breault D,Richardson G,von Zglinicki T,Saretzki G,Spyridopoulos I

MHCD1428 was used in flow cytometry and immunocytochemistry to study the effect of atorvastatin on telomerase activity in peripheral blood mononuclear cells and T-lymphocytes

Wed Oct 01 00:00:00 EDT 2014

Enlarged memory T-cell pool and enhanced Th1-type responses in chronic myeloid leukemia patients who have successfully discontinued IFN-α monotherapy.

PloS one

Ilander M,Kreutzman A,Rohon P,Melo T,Faber E,Porkka K,Vakkila J,Mustjoki S

MHCD1428 was used in flow cytometry to examine the function of NK- and T-cells in order to understand how chronic myeloid leukemia patients maintain the treatment response after IFN-α discontinuation.

Wed Oct 15 00:00:00 EDT 2014

Unexpected heterogeneity of multifunctional T cells in response to superantigen stimulation in humans.

Clinical immunology (Orlando, Fla.)

McArthur MA,Sztein MB

MHCD1428 was used in Flow cytometry/Cell sorting to investigate the mechanisms by which Staphylococcal enterotoxin B and other superantigens cause toxic shock syndrome.

Fri Feb 01 00:00:00 EST 2013

Quantitative and qualitative differences in the T cell response to HIV in uninfected Ugandans exposed or unexposed to HIV-infected partners.

Journal of virology

Pala P,Serwanga J,Watera C,Ritchie AJ,Moodie Z,Wang M,Goonetilleke N,Birabwa E,Hughes P,Senkaali D,Nakiboneka R,Grosskurth H,Haynes B,McMichael A,Kaleebu P

MHCD1428 was used in flow cytometry to characterize T cell responses of HIV-exposed, yet persistently uninfected individuals.

Thu Aug 01 00:00:00 EDT 2013

Monocytic AML cells inactivate antileukemic lymphocytes: role of NADPH oxidase/gp91(phox) expression and the PARP-1/PAR pathway of apoptosis.

Blood

Aurelius J,Thorén FB,Akhiani AA,Brune M,Palmqvist L,Hansson M,Hellstrand K,Martner A

MHCD1428 was used in Flow cytometry/Cell sorting to suggest a novel mechanism by which monocytic AML cells evade cell-mediated immunity.

Thu Jun 14 00:00:00 EDT 2012

A novel method for autophagy detection in primary cells: impaired levels of macroautophagy in immunosenescent T cells.

Autophagy

Phadwal K,Alegre-Abarrategui J,Watson AS,Pike L,Anbalagan S,Hammond EM,Wade-Martins R,McMichael A,Klenerman P,Simon AK

MHCD1428 was used in flow cytometry to develop and validate a novel system for high throughput quantitation of autophagy in primary human leukocytes.

Sun Apr 01 00:00:00 EDT 2012

Heterogeneity of multifunctional IL-17A producing S. Typhi-specific CD8+ T cells in volunteers following Ty21a typhoid immunization.

PloS one

McArthur MA,Sztein MB

MHCD1428 was used in flow cytometry to explore the multifunctional IL-17A responses against S. Typhi antigens in T memory subsets.

Tue Oct 02 00:00:00 EDT 2012

Anti-CD8 antibodies can trigger CD8+ T cell effector function in the absence of TCR engagement and improve peptide-MHCI tetramer staining.

Journal of immunology (Baltimore, Md. : 1950)

Clement M,Ladell K,Ekeruche-Makinde J,Miles JJ,Edwards ES,Dolton G,Williams T,Schauenburg AJ,Cole DK,Lauder SN,Gallimore AM,Godkin AJ,Burrows SR,Price DA,Sewell AK,Wooldridge L

MHCD1428 was used in flow cytometry to test if anti-CD8 Abs induce effector function.

Fri Jul 15 00:00:00 EDT 2011

Bioinformatics

Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

View more View less

Gene Aliases: CD14

View more View less

UniProt ID: (Human) P08571

View more View less

Entrez Gene ID: (Human) 929

View more View less

Function(s)

lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding transmembrane signal receptor

Process(es)

MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway receptor-mediated endocytosis phagocytosis apoptotic process inflammatory response cell surface receptor signaling pathway I-kappaB kinase/NF-kappaB signaling response to heat lipopolysaccharide-mediated signaling pathway response to magnesium ion positive regulation of type I interferon production positive regulation of interferon-gamma production positive regulation of tumor necrosis factor production negative regulation of MyD88-independent toll-like receptor signaling pathway toll-like receptor 4 signaling pathway response to tumor necrosis factor TRIF-dependent toll-like receptor signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway innate immune response response to ethanol positive regulation of endocytosis positive regulation of cytokine secretion response to electrical stimulus necroptotic process cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide apoptotic signaling pathway

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

CD14 Monoclonal Antibody (TuK4), Pacific Blue™

CD14 Antibody (MHCD1428) in Flow

Product Details

MHCD1428

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

We're here to help

Your items have has been added!