Invitrogen

CD14 Monoclonal Antibody (TuK4), APC

7 References

View all (206) CD14 antibodies

Datasheet

Protocols

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Datasheet

Protocols

Questions & Answers

Antibody Testing Data (1)

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CD14 Antibody (MHCD1405) in Flow

Human peripheral blood monocytes stained using APC of anti-human CD14 monoclonal antibody (clone Tuk4). The negative control profiles represent unstained cells.

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Product Details

MHCD1405

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 7 publications 7 publications

Product Specifications

Species Reactivity

Human

Published species

Human, Mouse

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

TuK4

Immunogen

Human CD14

Conjugate

APC APC APC

Excitation/Emission Max

651/660 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with sucrose, 4mg/mL BSA

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10372796

Product Specific Information

Allophycocyanin (APC) is a stable and highly soluble phycobiliprotein that provides maximal absorbance and fluorescence without susceptibility to internal or external fluorescence quenching, thus providing exceptional quantum yields and molar extinction coefficients.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

Analyte Specific Reagent

How to use the Panel Builder

Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.

Product References

Characterization of INCB086550: A Potent and Novel Small-Molecule PD-L1 Inhibitor.

Cancer discovery

Koblish HK,Wu L,Wang LS,Liu PCC,Wynn R,Rios-Doria J,Spitz S,Liu H,Volgina A,Zolotarjova N,Kapilashrami K,Behshad E,Covington M,Yang YO,Li J,Diamond S,Soloviev M,O'Hayer K,Rubin S,Kanellopoulou C,Yang G,Rupar M,DiMatteo D,Lin L,Stevens C,Zhang Y,Thekkat P,Geschwindt R,Marando C,Yeleswaram S,Jackson J,Scherle P,Huber R,Yao W,Hollis G

MHCD1405 was used in Flow Cytometry to identify and characterise INCB086550, a novel, oral, small-molecule programmed cell death protein 1 (PD-1) inhibitor.

Thu Jun 02 00:00:00 EDT 2022

BRAF^V600E-induced senescence drives Langerhans cell histiocytosis pathophysiology.

Nature medicine

Bigenwald C,Le Berichel J,Wilk CM,Chakraborty R,Chen ST,Tabachnikova A,Mancusi R,Abhyankar H,Casanova-Acebes M,Laface I,Akturk G,Jobson J,Karoulia Z,Martin JC,Grout J,Rafiei A,Lin H,Manz MG,Baccarini A,Poulikakos PI,Brown BD,Gnjatic S,Lujambio A,McClain KL,Picarsic J,Allen CE,Merad M

MHCD1405 was used in Flow cytometry/Cell sorting to identify senescent cells as a new target for the treatment of Langerhans cell histiocytosis.

Sat May 01 00:00:00 EDT 2021

BRAF^V600E-induced senescence drives Langerhans cell histiocytosis pathophysiology.

Nature medicine

MHCD1405 was used in Flow cytometry/Cell sorting to identify senescent cells as a new target for the treatment of Langerhans cell histiocytosis.

Sat May 01 00:00:00 EDT 2021

A Framework to Identify Antigen-Expanded T Cell Receptor Clusters Within Complex Repertoires.

Frontiers in immunology

Ceglia V,Kelley EJ,Boyle AS,Zurawski S,Mead HL,Harms CE,Blanck JP,Flamar AL,Kirschman JH,Ogongo P,Ernst JD,Levy Y,Zurawski G,Altin JA

MHCD1405 was used in Flow cytometry/Cell sorting to show that clonotypes within each CET respond to the same epitope, and that protein antigens stimulate multiple CETs reactive to constituent peptides.

Fri Feb 04 00:00:00 EST 2022

AllergoOncology: Generating a canine anticancer IgE against the epidermal growth factor receptor.

The Journal of allergy and clinical immunology

Fazekas-Singer J,Singer J,Ilieva KM,Matz M,Herrmann I,Spillner E,Karagiannis SN,Jensen-Jarolim E

MHCD1405 was used in Flow cytometry/Cell sorting to evaluate the cytotoxic killing potency of can225IgE-l by MPT-1 canine mast cells against A431 and measured specific tumor cell killing.

Sat Sep 01 00:00:00 EDT 2018

Characterization of porous, dexamethasone-releasing polyurethane coatings for glucose sensors.

Acta biomaterialia

Vallejo-Heligon SG,Klitzman B,Reichert WM

MHCD1405 was used in Flow cytometry/Cell sorting to characterise porous, dexamethasone-releasing polyurethane coatings for glucose sensors.

Sat Nov 01 00:00:00 EDT 2014

A microfabricated electrical differential counter for the selective enumeration of CD4+ T lymphocytes.

Lab on a chip

Watkins NN,Sridhar S,Cheng X,Chen GD,Toner M,Rodriguez W,Bashir R

MHCD1405 was used in Flow cytometry/Cell sorting to develop a microfabricated biochip that enumerates CD4+ T lymphocytes from leukocyte populations, with potential applications in quantitative HIV/AIDS diagnostics.

Thu Apr 21 00:00:00 EDT 2011

Inhibition of the Bcl-xL deamidation pathway in myeloproliferative disorders.

The New England journal of medicine

Zhao R,Follows GA,Beer PA,Scott LM,Huntly BJ,Green AR,Alexander DR

MHCD1405 was used in Flow cytometry/Cell sorting to study how BCR-ABL and mutant JAK2 inhibit the Bcl-x(L) deamidation pathway and the apoptotic response to DNA damage in primary cells from patients with CML or polycythemia vera.

Thu Dec 25 00:00:00 EST 2008

Bioinformatics

Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

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Gene Aliases: CD14

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UniProt ID: (Human) P08571

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Entrez Gene ID: (Human) 929

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Function(s)

lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding transmembrane signal receptor

Process(es)

MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway receptor-mediated endocytosis phagocytosis apoptotic process inflammatory response cell surface receptor signaling pathway I-kappaB kinase/NF-kappaB signaling response to heat lipopolysaccharide-mediated signaling pathway response to magnesium ion positive regulation of type I interferon production positive regulation of interferon-gamma production positive regulation of tumor necrosis factor production negative regulation of MyD88-independent toll-like receptor signaling pathway toll-like receptor 4 signaling pathway response to tumor necrosis factor TRIF-dependent toll-like receptor signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway innate immune response response to ethanol positive regulation of endocytosis positive regulation of cytokine secretion response to electrical stimulus necroptotic process cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide apoptotic signaling pathway

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD14 Monoclonal Antibody (TuK4), APC

CD14 Antibody (MHCD1405) in Flow

Product Details

MHCD1405

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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