Invitrogen

CD14 Monoclonal Antibody (TuK4), TRI-COLOR™

Advanced Verification

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

8 Published Figures

13 References

View all (206) CD14 antibodies

Datasheet

Protocols

Questions & Answers

Datasheet

Protocols

Questions & Answers

FIGURE: 1 / 10

CD14 Antibody (MHCD1406) in Flow

Human peripheral blood monocytes stained using TRI-COLOR® of anti-human CD14 monoclonal antibody (clone Tuk4). The negative control profiles represent unstained cells.

Product Image Gallery

CD14 Antibody — Figure 2 EP induces differentiation of leukemia cell lines. (A) Representative morphology of HL60 cells treated for 72 hours without EP, with EP, or with TPO shown at 20x (left panel) and 63x (right panel). Cells treated with EP portray reorganization of the nuclear contents compared with untreated cells (red arrows) and cells treated with TPO. (B) The fold change of FACS MFI +- SD (n = 3) of CD11B expression (i) and CD14 (ii) in HL60 cells treated with the above conditions relative to untreated cells for 72 hours. * P < .05; ** P < .01; *** P < .001. FACS analysis of CD11B expression (iii) in untreated HL60 cells (blue line) versus cells treated with 5 mug/mL of EP (orange line) for 72 hours. FACS analysis of CD14 expression (iv) in untreated HL60 cells (green line) versus cells treated with 5 mug/mL of EP (orange line) for 72 hours. CD11B and CD14 are overexpressed with EP treatment. (C) Representative morphology of U937 cells and URE cells treated for 72 hours under the above conditions. U937 cells treated with EP demonstrate increased size with increased vacuoles in the cytoplasm. (D) The fold change of FACS MFI +- SD (n = 3) of CD11B expression in U937 cells and URE cells treated with or without EP for 72 hours. * P < .05; ** P < .01. {TM}

Product Details

MHCD1406

Applications

Tested Dilution

Publications

Flow Cytometry (Flow)

Assay-Dependent

View 11 publications 11 publications

Miscellaneous PubMed (Misc)

View 2 publications 2 publications

Product Specifications

Species Reactivity

Human

Published species

Dog, Human

Host/Isotype

Mouse / IgG2a

Class

Monoclonal

Type

Antibody

Clone

TuK4

Immunogen

Human CD14

Conjugate

TRI-COLOR™ TRI-COLOR™ TRI-COLOR™

Excitation/Emission Max

567/671 nm View spectra

Form

Liquid

Purification

purified

Storage buffer

PBS with 10% sucrose

Contains

0.1% sodium azide

Storage conditions

4° C, store in dark

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_10373566

Product Specific Information

The TRI-COLOR® conjugate is a tandem R-phycoerythrin (PE)-Cy5® conjugate that permits simultaneous multicolor labeling and detection of multiple targets with excitation by a single excitation source-the 488 nm line of the argon-ion laser.

Target Information

CD14 is a 55 kDa GPI-anchored glycoprotein that is constitutively expressed on the surface of mature monocytes, macrophages, and neutrophils. CD14 also serves as a multifunctional lipopolysaccharide receptor, and is released to the serum both as a secreted and enzymatically cleaved GPI-anchored form. CD14 binds lipopolysaccharide molecule in a reaction catalyzed by lipopolysaccharide-binding protein (LBP), an acute phase serum protein. The soluble sCD14 can discriminate slight structural differences between lipopolysaccharides and is important for neutralization of serum allochthonous lipopolysaccharides by reconstituted lipoprotein particles. Further, CD14 has been shown to bind apoptotic cells, and can affect allergic, inflammatory and infectious processes. Alternative splicing results in multiple transcript variants encoding the same CD14 isoform. Diseases associated with CD14 dysfunction include mycobacterium chelonae infection and Croup.

Analyte Specific Reagent

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Product References

A molecular cell atlas of the human lung from single-cell RNA sequencing.

Nature

Travaglini KJ,Nabhan AN,Penland L,Sinha R,Gillich A,Sit RV,Chang S,Conley SD,Mori Y,Seita J,Berry GJ,Shrager JB,Metzger RJ,Kuo CS,Neff N,Weissman IL,Quake SR,Krasnow MA

MHCD1406 was used in Flow cytometry/Cell sorting to create an extensive cell atlas of the human lung by droplet- and plate-based single-cell RNA sequencing of approximately 75,000 human cells across all lung tissue compartments and circulating blood, combined with a multi-pronged cell annotation approach.

Sun Nov 01 00:00:00 EDT 2020

Adjuvanted HIV-1 vaccine promotes antibody-dependent phagocytic responses and protects against heterologous SHIV challenge.

PLoS pathogens

Om K,Paquin-Proulx D,Montero M,Peachman K,Shen X,Wieczorek L,Beck Z,Weiner JA,Kim D,Li Y,Mdluli T,Shubin Z,Bryant C,Sharma V,Tokarev A,Dawson P,White Y,Appelbe O,Klatt NR,Tovanabutra S,Estes JD,Matyas GR,Ferrari G,Alving CR,Tomaras GD,Ackerman ME,Michael NL,Robb ML,Polonis V,Rolland M,Eller MA,Rao M,Bolton DL

Published figure using CD14 monoclonal antibody (Product # MHCD1406) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Adjuvanted HIV-1 vaccine promotes antibody-dependent phagocytic responses and protects against heterologous SHIV challenge.

PLoS pathogens

Published figure using CD14 monoclonal antibody (Product # MHCD1406) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

Antibodies to a Conserved Influenza Head Interface Epitope Protect by an IgG Subtype-Dependent Mechanism.

Cell

Watanabe A,McCarthy KR,Kuraoka M,Schmidt AG,Adachi Y,Onodera T,Tonouchi K,Caradonna TM,Bajic G,Song S,McGee CE,Sempowski GD,Feng F,Urick P,Kepler TB,Takahashi Y,Harrison SC,Kelsoe G

MHCD1406 was used in Flow cytometry/Cell sorting to characterize a previously unrecognized epitope of influenza hemagglutinin (HA) that is immunogenic in humans and conserved among influenza subtypes.

Thu May 16 00:00:00 EDT 2019

CBX7 Induces Self-Renewal of Human Normal and Malignant Hematopoietic Stem and Progenitor Cells by Canonical and Non-canonical Interactions.

Cell reports

Jung J,Buisman SC,Weersing E,Dethmers-Ausema A,Zwart E,Schepers H,Dekker MR,Lazare SS,Hammerl F,Skokova Y,Kooistra SM,Klauke K,Poot RA,Bystrykh LV,de Haan G

MHCD1406 was used in Flow cytometry/Cell sorting to demonstrate that, among all five CBX Polycomb proteins, only CBX7 possesses the ability to control self-renewal of human hematopoietic stem and progenitor cells.

Tue Feb 12 00:00:00 EST 2019

Ultra-High-Frequency Reprogramming of Individual Long-Term Hematopoietic Stem Cells Yields Low Somatic Variant Induced Pluripotent Stem Cells.

Cell reports

Wang K,Guzman AK,Yan Z,Zhang S,Hu MY,Hamaneh MB,Yu YK,Tolu S,Zhang J,Kanavy HE,Ye K,Bartholdy B,Bouhassira EE

MHCD1406 was used in Flow cytometry/Cell sorting to suggest LT-HSCs may become the preferred cell source for the production of clinical-grade iPSCs.

Tue Mar 05 00:00:00 EST 2019

IL1RAP potentiates multiple oncogenic signaling pathways in AML.

The Journal of experimental medicine

Mitchell K,Barreyro L,Todorova TI,Taylor SJ,Antony-Debré I,Narayanagari SR,Carvajal LA,Leite J,Piperdi Z,Pendurti G,Mantzaris I,Paietta E,Verma A,Gritsman K,Steidl U

MHCD1406 was used in Flow cytometry/Cell sorting to show that targeting of interleukin-1 receptor accessory protein via RNA interference, genetic deletion, or antibodies inhibits acute myeloid leukaemia pathogenesis in vitro and in vivo.

Mon Jun 04 00:00:00 EDT 2018

IL1RAP potentiates multiple oncogenic signaling pathways in AML.

The Journal of experimental medicine

Mitchell K,Barreyro L,Todorova TI,Taylor SJ,Antony-Debré I,Narayanagari SR,Carvajal LA,Leite J,Piperdi Z,Pendurti G,Mantzaris I,Paietta E,Verma A,Gritsman K,Steidl U

Mon Jun 04 00:00:00 EDT 2018

Eradication of Canine Diffuse Large B-Cell Lymphoma in a Murine Xenograft Model with CD47 Blockade and Anti-CD20.

Cancer immunology research

Weiskopf K,Anderson KL,Ito D,Schnorr PJ,Tomiyasu H,Ring AM,Bloink K,Efe J,Rue S,Lowery D,Barkal A,Prohaska S,McKenna KM,Cornax I,O'Brien TD,O'Sullivan MG,Weissman IL,Modiano JF

MHCD1406 was used in Flow cytometry/Cell sorting to demonstrate, using a mouse xenograft model, the effectiveness of combining CD47-blockade with anti-CD20 as treatment against canine lymphoma.

Thu Dec 01 00:00:00 EST 2016

Eltrombopag inhibits the proliferation of leukemia cells via reduction of intracellular iron and induction of differentiation.

Blood

Roth M,Will B,Simkin G,Narayanagari S,Barreyro L,Bartholdy B,Tamari R,Mitsiades CS,Verma A,Steidl U

MHCD1406 was used in flow cytometry to examine the effects of eltrombopag on leukemia cell proliferation and the mechanism of its antiproliferative effects.

Thu Jul 12 00:00:00 EDT 2012

Uncoupling of proliferation and cytokines from suppression within the CD4+CD25+Foxp3+ T-cell compartment in the 1st year of human type 1 diabetes.

Diabetes

Hughson A,Bromberg I,Johnson B,Quataert S,Jospe N,Fowell DJ

MHCD1406 was used in Flow cytometry/Cell sorting to determine the relative proportions of effector and regulatory T cells in the CD4+CD25+Foxp3+ T-cell compartment in varying type 1 diabetes disease states.

Mon Aug 01 00:00:00 EDT 2011

Cytokine signatures of innate and adaptive immunity in 17DD yellow fever vaccinated children and its association with the level of neutralizing antibody.

The Journal of infectious diseases

Luiza-Silva M,Campi-Azevedo AC,Batista MA,Martins MA,Avelar RS,da Silveira Lemos D,Bastos Camacho LA,de Menezes Martins R,de Lourdes de Sousa Maia M,Guedes Farias RH,da Silva Freire M,Galler R,Homma A,Leite Ribeiro JG,Campos Lemos JA,Auxiliadora-Martins M,Eloi-Santos SM,Teixeira-Carvalho A,Martins-Filho OA

MHCD1406 was used in flow cytometry to discuss the live attenuated yellow fever vaccines.

Thu Sep 15 00:00:00 EDT 2011

Abnormal cytokine production by bone marrow stromal cells of multiple myeloma patients in response to RPMI8226 myeloma cells.

Archivum immunologiae et therapiae experimentalis

Zdzisińska B,Bojarska-Junak A,Dmoszyńska A,Kandefer-Szerszeń M

MHCD1406 was used in Flow cytometry/Cell sorting to determine the source of the differences in expression of extracellular matrix compounds and cytokine production between bone marrow stromal cells of healthy and multiple myeloma patients.

Wed Sep 03 00:00:00 EDT 2008

The first alpha helix of interleukin (IL)-2 folds as a homotetramer, acts as an agonist of the IL-2 receptor beta chain, and induces lymphokine-activated killer cells.

The Journal of experimental medicine

Eckenberg R,Rose T,Moreau JL,Weil R,Gesbert F,Dubois S,Tello D,Bossus M,Gras H,Tartar A,Bertoglio J,Chouaïb S,Goldberg M,Jacques Y,Alzari PM,Thèze J

MHCD1406 was used in flow cytometry to characterize a chemically synthesized fragment of IL-2.

Mon Feb 07 00:00:00 EST 2000

Blister fluid T lymphocytes during toxic epidermal necrolysis are functional cytotoxic cells which express human natural killer (NK) inhibitory receptors.

Clinical and experimental immunology

Le Cleach L,Delaire S,Boumsell L,Bagot M,Bourgault-Villada I,Bensussan A,Roujeau JC

MHCD1406 was used in flow cytometry to characterize the cells present in the cutaneous blister fluid patients with toxic epidermal necrolysis.

Sat Jan 01 00:00:00 EST 2000

Bioinformatics

Protein Aliases: CD 14; CD14; cd14 monocyte; LPSR antibody; Monocyte differentiation antigen CD14; My23 antigen; Myeloid cell-specific leucine-rich glycoprotein; sCD14; soluble CD14

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Gene Aliases: CD14

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UniProt ID: (Human) P08571

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Entrez Gene ID: (Human) 929

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Function(s)

lipopolysaccharide binding opsonin receptor activity protein binding peptidoglycan receptor activity lipoteichoic acid binding transmembrane signal receptor

Process(es)

MyD88-dependent toll-like receptor signaling pathway MyD88-independent toll-like receptor signaling pathway receptor-mediated endocytosis phagocytosis apoptotic process inflammatory response cell surface receptor signaling pathway I-kappaB kinase/NF-kappaB signaling response to heat lipopolysaccharide-mediated signaling pathway response to magnesium ion positive regulation of type I interferon production positive regulation of interferon-gamma production positive regulation of tumor necrosis factor production negative regulation of MyD88-independent toll-like receptor signaling pathway toll-like receptor 4 signaling pathway response to tumor necrosis factor TRIF-dependent toll-like receptor signaling pathway toll-like receptor TLR1:TLR2 signaling pathway toll-like receptor TLR6:TLR2 signaling pathway innate immune response response to ethanol positive regulation of endocytosis positive regulation of cytokine secretion response to electrical stimulus necroptotic process cellular response to lipopolysaccharide cellular response to lipoteichoic acid cellular response to diacyl bacterial lipopeptide cellular response to triacyl bacterial lipopeptide apoptotic signaling pathway

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If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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CD14 Monoclonal Antibody (TuK4), TRI-COLOR™

CD14 Antibody (MHCD1406) in Flow

Product Details

MHCD1406

Flow Cytometry (Flow)

Miscellaneous PubMed (Misc)

Species Reactivity

Published species

Host/Isotype

Class

Type

Clone

Immunogen

Conjugate

Excitation/Emission Max

Form

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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