Invitrogen

T-bet Monoclonal Antibody (eBio4B10 (4B10)), Alexa Fluor™ 488, eBioscience™

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T-bet Antibody (53-5825-82) in Flow

Normal human peripheral blood cells were stained intracellularly, using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol, with CD8a Monoclonal Antibody, PE-Cyanine7 (Product # 25-0088-42) and 0.125 µg of Mouse IgG1 kappa Isotype Control, Alexa Fluor 488 (Product # 53-4714-80) (left) or 0.125 µg of T-bet Monoclonal Antibody, Alexa Fluor 488 (right). Cells in the lymphocyte gate were used for analysis.

T-bet Antibody in Immunocytochemistry (ICC/IF)

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Product Details

53-5825-82

Applications

Tested Dilution

Publications

Western Blot (WB)

View 1 publication 1 publication

Immunocytochemistry (ICC/IF)

View 1 publication 1 publication

Flow Cytometry (Flow)

0.25 µg/test

View 18 publications 18 publications

Product Specifications

Species Reactivity

Human, Mouse, Rhesus monkey

Published species

Not Applicable

Host/Isotype

Mouse / IgG1, kappa

Recommended Isotype Control

Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), Alexa Fluor™ 488, eBioscience™

Class

Monoclonal

Type

Antibody

Clone

eBio4B10 (4B10)

Conjugate

Alexa Fluor™ 488 Alexa Fluor™ 488 Alexa Fluor™ 488

Excitation/Emission Max

499/520 nm View spectra

Form

Liquid

Concentration

0.5 mg/mL

Purification

Affinity chromatography

Storage buffer

PBS, pH 7.2

Contains

0.09% sodium azide

Storage conditions

4° C, store in dark, DO NOT FREEZE!

Shipping conditions

Ambient (domestic); Wet ice (international)

RRID

AB_2815216

Product Specific Information

Description: The eBio4B10 monoclonal antibody reacts with mouse, rhesus moneky and human T-bet. T-bet is a Th1-specific T-box transcription factor critical to the development of the Th1 CD4+ T cell lineage. This is known based on the observations that T-Bet deficient mice have impaired Th1 cell development, and that ectopic expression of T-Bet results in development skewed to the Th1 lineage. T-Bet expression is induced by the Th1 cytokine IFN gamma, and T-Bet also regulates the expression of IFN gamma, likely, at least in part, through the modification of DNA accessibility and histone remodeling. In addition to IFN gamma, T-Bet is also known to regulate the expression of IL-12R beta and IL-2. Moreover, T-Bet plays a role in class-switch recombination in B-cells.

Applications Reported: This eBio4B10 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.

Applications Tested: This eBio4B10 antibody has been tested by intracellular staining followed by flow cytometric analysis of normal human peripheral blood cells using the Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00) and protocol. Please refer to "Staining Intracellular Antigens for Flow Cytometry, Protocol B: One step protocol for intracellular (nuclear) proteins" located at www.thermofisher.com/flowprotocols . This may be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 488 nm; Emission: 519 nm; Laser: Blue Laser.

Target Information

T-bet, also known as TBX21, is a T-cell specific transcription factor containing a single T-box DNA-binding domain. It controls the expression of the TH1 cytokine, interferon-gamma. Both Th1 and Th17 cells are crucial in immune regulation and autoimmune disease development. T-bet initiates Th1 lineage development from naive Th precursor cells and directs T cell differentiation to Th1 versus Th17. T-bet is cooperating with Stat4 (signal tranducer and activator of transcription 4) in programming chromatin architecture for Th1 gene expression. Genetic variations in T-bet are associated with susceptibility to aspirin-induced asthma and nasal polyps.

For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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Product References

Perinatal murine cytomegalovirus infection reshapes the transcriptional profile and functionality of NK cells.

Nature communications

Rožmanić C,Lisnić B,Pribanić Matešić M,Mihalić A,Hiršl L,Park E,Lesac Brizić A,Indenbirken D,Viduka I,Šantić M,Adler B,Yokoyama WM,Krmpotić A,Juranić Lisnić V,Jonjić S,Brizić I

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Thu Oct 12 00:00:00 EDT 2023

The Potential of Dendritic-Cell-Based Vaccines to Modulate Type 3 Innate Lymphoid Cell Populations.

International journal of molecular sciences

Chan L,Mehrani Y,Minott JA,Bridle BW,Karimi K

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Thu Jan 26 00:00:00 EST 2023

GM-CSF-activated human dendritic cells promote type 1 T follicular helper cell polarization in a CD40-dependent manner.

Journal of cell science

Korniotis S,Saichi M,Trichot C,Hoffmann C,Amblard E,Viguier A,Grondin S,Noel F,Mattoo H,Soumelis V

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Tue Nov 01 00:00:00 EDT 2022

Agr2-associated ER stress promotes adherent-invasive E. coli dysbiosis and triggers CD103⁺ dendritic cell IL-23-dependent ileocolitis.

Cell reports

Viladomiu M,Khounlotham M,Dogan B,Lima SF,Elsaadi A,Cardakli E,Castellanos JG,Ng C,Herzog J,Schoenborn AA,Ellermann M,Liu B,Zhang S,Gulati AS,Sartor RB,Simpson KW,Lipkin SM,Longman RS

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Tue Nov 15 00:00:00 EST 2022

ADAM12 is a costimulatory molecule that determines Th1 cell fate and mediates tissue inflammation.

Cellular & molecular immunology

Liu Y,Bockermann R,Hadi M,Safari I,Carrion B,Kveiborg M,Issazadeh-Navikas S

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Sun Aug 01 00:00:00 EDT 2021

Imbalance between T helper 1 and regulatory T cells plays a detrimental role in experimental Parkinson's disease in mice.

The Journal of international medical research

Li W,Luo Y,Xu H,Ma Q,Yao Q

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Thu Apr 01 00:00:00 EDT 2021

Francisella tularensis induces Th1 like MAIT cells conferring protection against systemic and local infection.

Nature communications

Zhao Z,Wang H,Shi M,Zhu T,Pediongco T,Lim XY,Meehan BS,Nelson AG,Fairlie DP,Mak JYW,Eckle SBG,de Lima Moreira M,Tumpach C,Bramhall M,Williams CG,Lee HJ,Haque A,Evrard M,Rossjohn J,McCluskey J,Corbett AJ,Chen Z

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Fri Jul 16 00:00:00 EDT 2021

Heterologous arenavirus vector prime-boost overrules self-tolerance for efficient tumor-specific CD8 T cell attack.

Cell reports. Medicine

Bonilla WV,Kirchhammer N,Marx AF,Kallert SM,Krzyzaniak MA,Lu M,Darbre S,Schmidt S,Raguz J,Berka U,Vincenti I,Pauzuolis M,Kerber R,Hoepner S,Günther S,Magnus C,Merkler D,Orlinger KK,Zippelius A,Pinschewer DD

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Tue Mar 16 00:00:00 EDT 2021

Targeting Phosphatidylserine Enhances the Anti-tumor Response to Tumor-Directed Radiation Therapy in a Preclinical Model of Melanoma.

Cell reports

Budhu S,Giese R,Gupta A,Fitzgerald K,Zappasodi R,Schad S,Hirschhorn D,Campesato LF,De Henau O,Gigoux M,Liu C,Mazo G,Deng L,Barker CA,Wolchok JD,Merghoub T

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Tue Jan 12 00:00:00 EST 2021

Culture, expansion, and flow-cytometry-based functional analysis of pteropid bat MR1-restricted unconventional T cells.

STAR protocols

Sia WR,Hey YY,Foo R,Wang LF,Leeansyah E

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Fri Jun 18 00:00:00 EDT 2021

Role of nuclear localization in the regulation and function of T-bet and Eomes in exhausted CD8 T cells.

Cell reports

McLane LM,Ngiow SF,Chen Z,Attanasio J,Manne S,Ruthel G,Wu JE,Staupe RP,Xu W,Amaravadi RK,Xu X,Karakousis GC,Mitchell TC,Schuchter LM,Huang AC,Freedman BD,Betts MR,Wherry EJ

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Immunocytochemistry

Tue May 11 00:00:00 EDT 2021

C-Reactive Protein Suppresses the Th17 Response Indirectly by Attenuating the Antigen Presentation Ability of Monocyte Derived Dendritic Cells in Experimental Autoimmune Encephalomyelitis.

Frontiers in immunology

Shen ZY,Zheng Y,Pecsok MK,Wang K,Li W,Gong MJ,Wu F,Zhang L

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Western Blot

Thu Jul 01 00:00:00 EDT 2021

Graded RhoA GTPase Expression in Treg Cells Distinguishes Tumor Immunity From Autoimmunity.

Frontiers in immunology

Kalim KW,Yang JQ,Modur V,Nguyen P,Li Y,Zheng Y,Guo F

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Tue Jan 11 00:00:00 EST 2022

MiR-1165-3p Suppresses Th2 Differentiation via Targeting IL-13 and PPM1A in a Mouse Model of Allergic Airway Inflammation.

Allergy, asthma & immunology research

Wang Z,Ji N,Chen Z,Sun Z,Wu C,Yu W,Hu F,Huang M,Zhang M

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Tue Sep 01 00:00:00 EDT 2020

CD19-CAR-T Cells Bearing a KIR/PD-1-Based Inhibitory CAR Eradicate CD19⁺HLA-C1^- Malignant B Cells While Sparing CD19⁺HLA-C1⁺ Healthy B Cells.

Cancers

Tao L,Farooq MA,Gao Y,Zhang L,Niu C,Ajmal I,Zhou Y,He C,Zhao G,Yao J,Liu M,Jiang W

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Sun Sep 13 00:00:00 EDT 2020

Programming Multifaceted Pulmonary T Cell Immunity by Combination Adjuvants.

Cell reports. Medicine

Marinaik CB,Kingstad-Bakke B,Lee W,Hatta M,Sonsalla M,Larsen A,Neldner B,Gasper DJ,Kedl RM,Kawaoka Y,Suresh M

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Tue Sep 22 00:00:00 EDT 2020

Th2 Biased Immunity With Altered B Cell Profiles in Circulation of Patients With Sporotrichosis Caused by Sporothrix globosa.

Frontiers in immunology

Zu J,Yao L,Song Y,Cui Y,Guan M,Chen R,Zhen Y,Li S

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Mon May 31 00:00:00 EDT 2021

Adjuvant-free nanofiber vaccine induces in situ lung dendritic cell activation and T_H17 responses.

Science advances

Si Y,Tian Q,Zhao F,Kelly SH,Shores LS,Camacho DF,Sperling AI,Andrade MS,Collier JH,Chong AS

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Sat Aug 01 00:00:00 EDT 2020

FCRL5⁺ Memory B Cells Exhibit Robust Recall Responses.

Cell reports

Kim CC,Baccarella AM,Bayat A,Pepper M,Fontana MF

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Tue Apr 30 00:00:00 EDT 2019

Single-Cell Profiling Defines Transcriptomic Signatures Specific to Tumor-Reactive versus Virus-Responsive CD4⁺ T Cells.

Cell reports

Magen A,Nie J,Ciucci T,Tamoutounour S,Zhao Y,Mehta M,Tran B,McGavern DB,Hannenhalli S,Bosselut R

Published figure using T-bet monoclonal antibody (Product # 53-5825-82) in Flow Cytometry

Tue Dec 03 00:00:00 EST 2019

Bioinformatics

Protein Aliases: HGNC:11599; T-box 21; T-box expressed in T cells; T-box protein 21; T-box transcription factor TBX21; T-cell-specific T-box transcription factor T-bet; Transcription factor TBLYM

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Gene Aliases: T-bet; T-PET; TBET; TBLYM; TBT1; TBX21

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UniProt ID: (Human) Q9UL17, (Mouse) Q9JKD8

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Entrez Gene ID: (Rhesus monkey) 694044, (Human) 30009, (Mouse) 57765

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Function(s)

transcription factor activity, sequence-specific DNA binding transcription regulatory region DNA binding DNA binding protein binding Rel homology transcription factor

Process(es)

transcription, DNA-templated regulation of transcription, DNA-templated multicellular organism development response to virus T cell differentiation positive regulation of transcription, DNA-templated positive regulation of isotype switching to IgG isotypes cellular response to organic substance lymphocyte migration regulation of immune response

Performance Guarantee

If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

Learn more

T-bet Monoclonal Antibody (eBio4B10 (4B10)), Alexa Fluor™ 488, eBioscience™

T-bet Antibody (53-5825-82) in Flow

Product Details

53-5825-82

Western Blot (WB)

Immunocytochemistry (ICC/IF)

Flow Cytometry (Flow)

Species Reactivity

Published species

Host/Isotype

Recommended Isotype Control

Class

Type

Clone

Conjugate

Excitation/Emission Max

Form

Concentration

Purification

Storage buffer

Contains

Storage conditions

Shipping conditions

RRID

Product Specific Information

Target Information

How to use the Panel Builder

Bioinformatics

Performance Guarantee

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