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Invitrogen
TER-119 Monoclonal Antibody (TER-119), Alexa Fluor™ 660, eBioscience™
FIGURE: 1 / 1
TER-119 Antibody (606-5921-82) in Flow
C57BL/6 mouse bone marrow cells were stained with CD45 Monoclonal Antibody, eFluor 450 (Product # 48-0451-82) and 0.25 µg of Rat IgG2b kappa Isotype Control, Alexa Fluor 660 (Product # 606-4031-81) (left) or 0.25 µg of TER-119 Monoclonal Antibody, Alexa Fluor 660 (right). Total cells were used for analysis. This data was collected on a 5-laser Cytek Aurora full spectral cytometer.
Product Details
606-5921-82
Product Specifications
Species Reactivity
Mouse
Host/Isotype
Rat
/ IgG2b, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
TER-119
Conjugate
Excitation/Emission Max
663/691 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2896292
Product Specific Information
Description: The TER-119 monoclonal antibody reacts with mouse erythroid cells from early proerythroblast to mature erythrocyte stages. The TER-119 antigen is present in yolk sac, fetal and newborn liver, but is not expressed by cells carrying BFU-E and CFU-E activities. Several erythroleukemia cell lines tested so far are negative for expression of TER-119 antigen even after dimethylsulfoxide stimulation. Biochemical and molecular analysis of the TER-119 antigen indicate that this molecule is associated with the surface glycophorin A, but is not a typical glycophorin.
Applications Reported: This TER-119 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This TER-119 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This may be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Alexa Fluor 660 emits at 690 nm and is intended for use on spectral cytometers where it may be multiplexed with APC, Alexa Fluor 647 and Alexa Fluor 700.
Excitation: 662 nm; Emission: 690 nm; Laser: Red Laser
Target Information
TER-119 is a lineage marker for erythroid cells from early proerythroblast to mature erythrocyte stages in adult blood, spleen, and bone marrow. It is also present in yolk sac, and fetal and newborn liver. The TER-119 antigen is not expressed by cells of earlier erythroid development at BFU-e (blast-forming unit erythroid) stage or CFU-e (colony-forming unit erythroid) stage.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: Ly-76; Ly76; TER119
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