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Invitrogen
TLR4/MD-2 Complex Monoclonal Antibody (MTS510), Super Bright™ 436, eBioscience™
FIGURE: 1 / 7
TLR4/MD-2 Complex Antibody (62-9924-82) in Flow
Swiss Webster mouse bone marrow-derived dendritic cells were stained with 0.25 µg of Rat IgG2a kappa Isotype Control, Super Bright 436 (Product # 62-4321-82) (blue histogram) or 0.25 µg of TLR4/MD-2 Monoclonal Antibody, Super Bright 436 (purple histogram). Total viable cells were used for analysis, as determined by Fixable Viability Dye eFluor 780 (Product # 65-0865-18).
Product Details
62-9924-82
Applications
Tested Dilution
Publications
Product Specifications
Species Reactivity
Mouse
Published species
Not Applicable
Host/Isotype
Rat
/ IgG2a, kappa
Recommended Isotype Control
Class
Monoclonal
Type
Antibody
Clone
MTS510
Conjugate
Excitation/Emission Max
413/431 nm
View spectra
Form
Liquid
Concentration
0.2 mg/mL
Purification
Affinity chromatography
Storage buffer
PBS, pH 7.2, with BSA
Contains
0.09% sodium azide
Storage conditions
4° C, store in dark, DO NOT FREEZE!
Shipping conditions
Ambient (domestic); Wet ice (international)
RRID
AB_2762765
Product Specific Information
Description: The MTS510 monoclonal antibody reacts with the mouse Toll-like receptor 4 (TLR4)/MD-2 complex. At least ten members of the Toll family have been identified. This family of type I transmembrane proteins is characterized by an extracellular domain with leucine-rich repeats and a cytoplasmic domain with homology to the type I IL-1 receptor. Two of these receptors, TLR2 and TLR4, are pattern recognition receptors and signaling molecules in response to bacterial lipoproteins and have been implicated in innate immunity and inflammation. TLR4 physically associates with MD-2, and together with CD14, this complex is responsible for LPS recognition and signaling. In the mouse, TLR4 is expressed by thioglycolate-elicited peritoneal macrophages. Incubation of peritoneal macrophages with LPS results in down regulation of surface TLR4/MD-2. The TLR4 gene is defective in C3H/HeJ and C57BL/10ScCr mice, both of which have been well characterized as hyporesponders to LPS.
The MTS510 monoclonal antibody co-immunoprecipitates MD-2 (~30 kDa) and TLR4 (~100 kDa), and preferentially reacts with TLR4 that is associated with MD-2. In comparison, binding of the UT41 monoclonal antibody occurs with and without formation of the TLR4/MD-2 complex. Please contact eBioscience Technical Support for further information.
Applications Reported: This MTS510 antibody has been reported for use in flow cytometric analysis.
Applications Tested: This MTS510 antibody has been tested by flow cytometric analysis of mouse bone marrow derived dendritic cells. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Super Bright 436 can be excited with the violet laser line (405 nm) and emits at 436 nm. We recommend using a 450/50 bandpass filter, or equivalent. Please make sure that your instrument is capable of detecting this fluorochrome.
When using two or more Super Bright dye-conjugated antibodies in a staining panel, it is recommended to use Super Bright Complete Staining Buffer (Product # SB-4401) to minimize any non-specific polymer interactions. Please refer to the datasheet for Super Bright Staining Buffer for more information.
Excitation: 405 nm; Emission: 436 nm; Laser: Violet Laser
Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
Target Information
MD-2 is a member of the Toll/interleukin-1 receptor (TIR) family, a group of proteins that include the Toll-like receptors (TLRs). TLRs are signaling molecules that recognize different pathogen-associated molecular patterns (PAMPs) and serve as an important link between the innate and adaptive immune responses. TLR4, the major signaling receptor for lipopolysaccharide (LPS), requires the binding of MD-2 to its extracellular region for maximal response to LPS. The specificity of this response is determined by the species of MD-2; e.g., human MD-2 transfected into mouse cells can cause mouse TLR4 to react to LPS analogs that are normally antagonistic to human but not mouse TLR4.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
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Bioinformatics
Protein Aliases: ARMD10; CD284; ESOP-1; lipopolysaccharide response; Ly-96; Lymphocyte antigen 96; myeloid differentiation factor-2; myeloid differentiation protein-2; Protein MD-2; TLR-4; TOLL; Toll-like receptor 4
Gene Aliases: ESOP-1; Esop1; Lps; Ly87; Ly96; MD-2; MD2; Ran/M1; Rasl2-8; Tlr4
UniProt ID: (Mouse) Q9JHF9, (Mouse) Q9QUK6
Entrez Gene ID: (Mouse) 17087, (Mouse) 21898
pattern recognition receptor signaling pathway
immune system process
inflammatory response
lipopolysaccharide-mediated signaling pathway
positive regulation of lipopolysaccharide-mediated signaling pathway
response to lipopolysaccharide
detection of lipopolysaccharide
innate immune response
activation of MAPK activity
microglial cell activation
activation of innate immune response
toll-like receptor signaling pathway
B cell proliferation involved in immune response
nitric oxide production involved in inflammatory response
regulation of dendritic cell cytokine production
MyD88-dependent toll-like receptor signaling pathway
innate immune response-activating signal transduction
leukotriene metabolic process
response to oxidative stress
signal transduction
activation of NF-kappaB-inducing kinase activity
I-kappaB phosphorylation
response to bacterium
positive regulation of platelet activation
positive regulation of B cell proliferation
interferon-gamma production
negative regulation of interferon-gamma production
negative regulation of interleukin-17 production
negative regulation of interleukin-23 production
negative regulation of interleukin-6 production
negative regulation of tumor necrosis factor production
positive regulation of chemokine production
positive regulation of interferon-alpha production
positive regulation of interferon-beta production
positive regulation of interferon-gamma production
positive regulation of interleukin-1 production
positive regulation of interleukin-10 production
positive regulation of interleukin-12 production
positive regulation of interleukin-6 production
positive regulation of interleukin-8 production
positive regulation of tumor necrosis factor production
positive regulation of stress-activated MAPK cascade
toll-like receptor 4 signaling pathway
macrophage activation
positive regulation of NF-kappaB import into nucleus
positive regulation of tumor necrosis factor biosynthetic process
positive regulation of apoptotic process
positive regulation of I-kappaB kinase/NF-kappaB signaling
positive regulation of DNA binding
positive regulation of interleukin-12 biosynthetic process
positive regulation of MHC class II biosynthetic process
positive regulation of interferon-beta biosynthetic process
positive regulation of interleukin-1 biosynthetic process
positive regulation of interleukin-13 biosynthetic process
positive regulation of interleukin-6 biosynthetic process
positive regulation of interleukin-8 biosynthetic process
positive regulation of nitric oxide biosynthetic process
response to ethanol
mast cell activation
positive regulation of transcription from RNA polymerase II promoter
positive regulation of JNK cascade
positive regulation of lymphocyte proliferation
regulation of cytokine secretion
regulation of inflammatory response
positive regulation of inflammatory response
positive regulation of peptidyl-tyrosine phosphorylation
defense response to Gram-negative bacterium
positive regulation of sequence-specific DNA binding transcription factor activity
positive regulation of NF-kappaB transcription factor activity
positive regulation of nitric-oxide synthase biosynthetic process
regulation of sensory perception of pain
intestinal epithelial structure maintenance
positive regulation of macrophage cytokine production
negative regulation of ERK1 and ERK2 cascade
positive regulation of ERK1 and ERK2 cascade
positive regulation of nucleotide-binding oligomerization domain containing 1 signaling pathway
positive regulation of nucleotide-binding oligomerization domain containing 2 signaling pathway
response to fatty acid
cellular response to lipopolysaccharide
cellular response to lipoteichoic acid
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