Vanquish Tandem LC Systems

Vanquish Tandem LC System

The Thermo Scientific Vanquish Tandem LC Systems increase sample throughput for any LC or LC-MS analysis. These systems increase throughput and maximize detector output by using a second pump and intelligent column switching to eliminate the time required for column equilibration in a gradient elution.

Highlights

Increased sample throughput

Eliminates the time required for column reconditioning as well as idle time for your mass spectrometer or LC detector by using a dedicated reconditioning pump and a second column. Users can increase throughput by an average of 40% to improve laboratory output and revenue generation.

Reduced column carryover

During reconditioning, advanced column wash programs can reduce column carryover without sacrificing throughput.

Simplified method setup

Effortlessly convert your original gradient method for the tandem LC workflow with Thermo Scientific Chromeleon Chromatography Data System (CDS) wizards. Simply enter your standard gradient and the CDS automatically calculates all required parameters with compliance-ready data handling, even for LC-MS instruments.

Versatility in analyte detection

A variety of detection options ensure you can collect the most information from your separation. All detector options offer excellent sensitivity, including diode array, fluorescence or label-free detection of non-chromophoric substances via charged aerosol detection.

Easy and reliable tool-free connections

The Vanquish Tandem LC Systems are fitted with Thermo Scientific Viper Fingertight Fittings, which support near-zero dead volume connections and enable sharper peaks and tool-free setup.

CDS software

The Vanquish Tandem LC Systems seamlessly integrate with our Thermo Scientific Chromeleon Chromatography Data System (CDS) software to deliver superior compliance tools, networking capabilities, automation and data processing.

What is tandem LC-MS?

The tandem LC-MS system incorporates a second pump and intelligent column switching to allow you to maximize detector usage and regain the time lost from column reconditioning.

The diagram below illustrates the time required to run the same set of assays on a traditional single-channel HPLC system compared with the tandem LC or LC-MS system, identifying where time savings are achieved.

Compared to single-channel LC, the column reconditioning step in a tandem LC-MS workflow can be performed simultaneously on a separate flow path to save time.

System specifications

The table below provides an overview of the specifications for a standard setup of the Vanquish Tandem LC Systems. Not all specifications are listed. Your specifications may differ based on the components of your system. Find the specification sheets for the system components in the Component specifications, below.

Category	Vanquish Tandem LC Systems
System pressure, maximum	700, 1000 or 1500 bar, depends on system configuration
Flow range, maximum	0–5, 0-8 or 0-10 mL/min, depends on pumps selected
Pump options (default gradient delay volume)	Binary (35, 200, or 400 µL) Quaternary (679 µL) Dual (679 µL) Delay volume depends on pump(s) selected
Biocompatible	Yes, depends on system configuration
pH range	2–12, chloride concentration up to 1 mol/L
System design	Modular
Touchscreen user interface display compatible (optional)	Yes, depends on system configuration
Solvent monitoring system compatible (optional)	Yes, depends on system configuration
Customizable gradient delay volume to simplify method transfer	Yes, depends on system configuration
SmartInject technology for maximum injection precision and improved column lifetime	Yes
Injection volume range	Standard: Up to 0.01-25 or 0.01-100 µL (Vanquish Flex/Vanquish Horizon) Optional: Up to 0.01-100 or 0.01-1000 µL (Vanquish Core) Depends on autosampler selected
Sample cooling	Yes, 4-40°C dependent on sampler selected
Sample capacity (standard) Based on 12 mm OD vials (≤1.5 mL) for comparison	4 racks of 54 vials (≤ 1.5 mL) each, other rack formats available + capacity of 12 × 22.5 mm OD vials (≤ 10 mL) in the carousel
Sampler extension available (optional)	Yes, with Vanquish Charger
Column compartment thermostatting	Yes, 5-120°C, depends on column compartment selected Still air and forced air
Column compartment pre-heater	Active and passive Depends on system configuration
Column compartment post-column cooler	Yes Depends on column compartment selected
Compatible detectors	Vanquish Charged Aerosol Detector Vanquish Diode Array Detector Vanquish Fluorescence Detector Vanquish Multiple Wavelength Detector Vanquish Variable Wavelength Detector Refractive Index Detector Mass Spectrometer

Component specifications

Standard Vanquish Tandem LC Systems are made up of the following components with detail available below.

	Vanquish Horizon Tandem UHPLC System	Vanquish Flex Tandem UHPLC System	Vanquish Core Tandem HPLC System
Pump Download Specification Sheet	2 Vanquish Binary Pump H	2 Vanquish Binary Pump F or 2 Vanquish Quaternary Pump F or 1 Vanquish Dual Pump F	2 Vanquish Binary Pump C/CN or 2 Vanquish Quaternary Pump C/CN or 1 Vanquish Dual Pump C/CN
Autosampler Download Specification Sheet	Vanquish Split Sampler HT	Vanquish Split Sampler FT	Vanquish Split Sampler C or Vanquish Split Sampler CT
Extended sample capacity (optional) Download Specification Sheet	Vanquish Charger  (optional)
Column compartment Download Specification Sheet	Vanquish Column Compartment H		Vanquish Column Compartment C
Virtually zero dead volume system connections Download Specification Sheet	Viper UHPLC Fittings Kits
Detectors	1 Detector Visit our HPLC and UHPLC Detectors page