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Apoptosis Information
Many different approaches are used in the study of programmed cell death, or apoptosis. Cell death cascades are complex and dynamic, underscoring the importance of a multi-parametric approach for accurate assessment. This page features application notes, webinars and other useful educational resources to help you navigate the complex world of apoptosis research.
Related content
Apoptosis features
On-demand webinar
The meaning of life at the cellular level: detecting apoptosis with fluorescence
In this webinar, Thermo Fisher Scientific scientists will demonstrate several solutions using fluorescent probes to study key components of the apoptotic machinery.
Signaling pathways
Apoptosis pathways
Find information about the numerous and complex signaling pathways involved in the process of apoptosis including pathway schematics and overviews.
BioProbes articles
Journal club: measurement and characterization of apoptosis by flow cytometry
This journal club provides a review of a recently published methods chapter in Current Protocols by Telford and coauthors that offers detailed protocols for several methods to measure and characterize apoptosis using multiparametric flow cytometry. The article provides basic theory discussions, detailed reagent listings, step-by-step procedures, and guidance on analysis methods, while also identifying critical items to consider when performing these assays.
Multiplexable Click-iT Plus TUNEL Assays for in situ apoptosis detection
Here we describe the limitations of conventional TUNEL assays and introduce the Click-iT Plus TUNEL Assays for In Situ Apoptosis Detection. Click-iT Plus technology enables you to perform specific and sensitive TUNEL assays that can be multiplexed with other fluorescence-based cell function assays, including those that incorporate fluorescent proteins and a variety of dyes.
Apoptosis learning resources
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| Type | Title | Categories |
|---|---|---|
| Application note (2011) | Apoptosis detection: Apoptosis assays for the Attune Acoustic Focusing Cytometer | Alexa Fluor, apoptosis, Attune/Attune NxT, cell structure-mitochondria, cell structure-plasma membrane, flow cytometer/flow cytometry, fluorescent dyes |
| Application note (2013) | Multiplexed mitosis and apoptosis analysis | antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, particles |
| Application note (2014) | Elucidating the mechanism of cell death through the use of high content analysis | apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis |
| Application note (2015) | Fluorescent apoptosis evaluation on the Countess II FL Automated Cell Counter | apoptosis, automated cell counter, cell counting, Countess, EVOS, fluorescent dyes, viability |
| BioProbes articles (Issues 50– present day) | BioProbes Journal of Cell Biology Application | cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification |
| Molecular Probes Handbook | Assays for apoptosis and autophagy—Section 15.5 | apoptosis, autophagy, fluorescent dyes, fluorescence microscopy/fluorescence imaging, flow cytometer/flow cytometry |
| Protocol | Pharmacological induction of apoptosis with camptothecin | cell vitality, flow cytometry, high content analysis, imaging, microplate, apoptosis |
| Scientific poster (2006) | Utility of 405 nm-excitable dyes in high content screening using an Acumen Explorer Microplate Cytometer | Acumen Explorer, apoptosis, cell cycle, cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, violet laser-excited reagents |
| Scientific poster (2008) | Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents | Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis |
| Scientific poster (2008) | Novel Click-IT TUNEL Assay for detection of cell death | Alexa Fluor, antibodies, apoptosis, Click-iT, cytotoxicity, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, immunocytochemistry (ICC), viability |
| Scientific poster (2009) | A non-radioactive method for detecting nascent protein synthesis by high-content imaging utilizing click chemistry | Alexa Fluor, apoptosis, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, protein detection |
| Scientific poster (2009) | A simple, fast and quantitative single-step dead-cell indicator for flow cytometry | apoptosis, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability |
| Scientific poster (2009) | Dead cell stains in flow cytometry: A comprehensive analysis | apoptosis, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, viability |
| Scientific poster (2009) | Click chemistry-based assays for fluorescence microscopy and high content imaging | Alexa Fluor, apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid detection, protein detection, viability |
| Scientific poster (2010) | Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry | apoptosis, autophagy, caspase substrates, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress |
| Scientific poster (2010) | Acoustic focusing cytometry: Sensitivity and throughput | apoptosis, Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, phagocytosis |
| Scientific poster (2010) | Simultaneous analysis of cell death mechanisms and oxidative stress using live cell fluorescence microscopy | apoptosis, ArrayScan, BacMam technology, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader, oxidative stress |
| Scientific poster (2011) | Simultaneous analysis of cell death mechanisms and oxidative stress using high content imaging | Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2011) | Validation of high content imaging and analysis assays for cell health and cytotoxicity | antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, cell health, cell proliferation, CellLight, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, pH detection |
| Scientific poster (2011) | The next generation of cell-based imaging assays for apoptosis, autophagy and oxidative stress from Molecular Probes | Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2013) | Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health | apoptosis, ArrayScan, caspase substrates, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2015) | Colorimetric dual labeled EdU/BrdU technology demonstrates contextual information and dynamics of proliferation in tissue | apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC) |
| Scientific poster (2015) | Visualizing the life and death of cells: novel probes and platforms | apoptosis, ArrayScan, autophagy, cell death, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis |
| Scientific poster (2016) | Click colorimetric EdU proliferation and TUNEL: Click chemistry for brightfield microscopy | apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC) |
| Video | CellEvent Caspase 3/7 reagent and apoptosis detection HeLa cells were loaded with 50 nM TMRM (red) followed by 5 μM CellEvent Caspase 3/7 Substrate (green). Cells were then treated with 0.5 μM staurosporine to induce apoptosis. TMRM detects the mitochondrial membrane potential and the signal disappears as the apoptosis onsets. CellEvent detects apoptosis, with green signal. | apoptosis, cell health, CellEvent, fluorescence microscopy/fluorescence imaging, live-cell imaging |
| Video | Click-iT Plus TUNEL apoptosis assays The Click-iT Plus TUNEL assay detects apoptotic cells in tissue and cultured cell samples through the use of a small, highly specific labeling moiety and a bright fluorescent dye. After incorporation of the labeling moiety into DNA fragments, detection is achieved through a catalyzed "click" reaction using conditions mild enough to preserve the fluorescent signal from GFP or RFP. | apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging |
| Video | Time-lapse of cell migration and apoptosis during angiogenesis Angiogenesis pseudo-tube formation and apoptosis shown with time-lapse imaging. The time-lapse images were captured and assembled using an EVOS FL Auto Imaging System equipped with the EVOS Onstage Incubator. | angiogenesis, apoptosis, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator |
| Webinar | The meaning of life at the cellular level: detecting apoptosis with fluorescence In this webinar, Thermo Fisher Scientific scientists will demonstrate several solutions using fluorescent probes to study key components of the apoptotic machinery. The presentation includes: An overview of the features of apoptosis Key parameters that can be measured to assay apoptosis A comprehensive guide to available labeling and detection technologies for apoptosis research Tips and tricks to best implement those technologies | apoptosis, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent proteins |
| Webinar | An introduction to flow cytometric analysis, Part 2: Cell viability and apoptosis analysis In this free webinar, we will discuss flow cytometric analysis of apoptosis and identification of dead cells. Changes introduced by apoptosis can be tested with numerous assays measuring Membrane structure, Mitochondrial function, Metabolism, Caspase activity, Membrane integrity, and DNA fragmentation. We will also discuss dead cell identification using traditional impermeant nucleic acid dyes. | apoptosis, cell cycle, cell proliferation, flow cytometer/flow cytometry, multicolor flow cytometry, viability |
| Webinar | An introduction to flow cytometric analysis, Part 1: Cell proliferation analysis In this 2-part series, we will give an overview of tools and techniques using Invitrogen reagents for flow cytometric analysis of cell proliferation, viability, vitality, and apoptosis. | apoptosis, cell cycle, cell proliferation, flow cytometer/flow cytometry, multicolor flow cytometry, viability |
| Application note (2017) | Flow cytometry analysis of dose response for apoptosis induction | apoptosis, Attune/Attune NxT, cancer, flow cytometer/flow cytometry, Autosampler, cell toxicity, CellEvent Caspase-3/7 Green |
| Protocols | Annexin V staining protocol for flow cytometry (Invitrogen eBioscience reagents) | Apoptosis, flow cytometry |
| Scientific poster (2018) | Fluorescent hypoxia sensor for flow cytometry | Apoptosis, Attune/Attune NxT, brightfield microscopy, EVOS, flow cytometry, hypoxia, immunology |
| Scientific poster (2013) | Monitor caspase 3/7 activity without cell fixation: A novel apoptosis reagent | Alexa Fluor, apoptosis, Attune, Attune NxT, caspase substrates, cells, fluorescent dyes, multicolor flow cytometry, flow cytometry |
| Scientific poster (2006) | Novel violet-excited reagents for detection of viability and vitality | Alexa Fluor, cell health, CellTrace, flow cytometry, fluorescent dyes, viability, violet laser-excited reagents |
| CYToMORE lecture video (2016) | Novel reagent solutions for flow cytometry Presented by Jolene Bradford, Thermo Fisher Scientific, USA | Attune NxT, flow cytometry assays |
Resources
BioProbes Journal of Cell Biology Applications—Stay up-to-date with highlights of the latest breakthroughs and get information about new technologies and products.
Tools
Fluorescence SpectraViewer—This is an online tool for the visualization of the excitation and emission of fluorescent reagents. This tool allows for checking spectral compatibility for multiple fluorophores.
Flow Cytometry Panel Builder—Design your flow cytometry panel with this online tool for a simplified, customizable experience to fit your needs.
For Research Use Only. Not for use in diagnostic procedures.