CellInsight CX7 LZR Pro HCS Platform

Laser-based fluorescence as well as brightfield illumination and confocal capabilities

Catalog numbers: HCSDCX7LZRPRO

 

The Thermo Scientific CellInsight CX7 LZR Pro platform seamlessly integrates advanced screening technologies to deliver superior performance for lightning-fast discovery across the life sciences. Designed to meet the challenging needs of emerging life science models, the CX7 LZR Pro incorporates both laser-based fluorescence illumination and integrated Nipkow spinning-disc confocal technology for publication quality imaging of a broad range of samples from simple monolayers to thick spheroid samples. 

 

Dual mode software- and laser-based autofocusing methods contribute to the platform’s diverse capabilities to investigate simple to complex models. On-the-fly phenotyping enables parallel image capture and analysis for multiplexed cytometry measurements in real time. The Thermo Scientific Amira software option enables 3-dimensional analysis of the most complex research models from 3D morphological tracing in neurons to co-localizations of immune and cancer cells within tumors to enable next-generation translational research. 

 

Browse the features, specifications, videos, sample data, and reagent selection guide sections below. 

Features

In addition to the common features of all high-content screening platforms, the CellInsight CX7 LZR Pro platform offers:

Next-generation sCMOS camera

The CX7 LZR Pro platform has been upgraded with a sCMOS camera, offering greater than 95% peak quantum efficiency and low background 1.0e-ready noise. This quantum efficiency further accelerates high-throughput performance thanks to reduced exposure times across all wavelengths. Boost your experiment’s assay window, with up to 65,536 shades of grey detection while not sacrificing background noise thanks to the -20˚C cooling.

 

Introducing Olympus X-line objectives to the CX7 Pro

The CX7 LZR Pro platform now offers Olympus X-line objectives to further improve the instrument's imaging quality and assay performance. Publication-quality imaging is now standard using the new CX7 LZR Pro platform.  

 

Laser-based illumination 

Seven independent lasers aligned with multiplexable fluorescent cellular labeling for the best performance in speed and image quality.

Laser illumination provides flatter and brighter images with low background.

Laser light engine

Illuminates the field evenly resulting in the best image quality appropriate for quantitation.

Up to 6X increase in 3D confocal screening times compared to LED illumination.

 

Short exposure times and laser autofocus capabilities

Speeds up the acquisition of images. 

 

Near-IR (785 nm) laser excitation

Expand your multiplexing capabilities with near-IR (785 nm) laser excitation.

 

Autofocus

Dual Mode Autofocusing Software and Laser Autofocus modes accommodate the widest variety of sample types.

 

Extremely bright illumination

Penetrate thick samples during widefield or confocal 3D imaging.

Specifications

Category Attribute Description
Optics Camera

Photometrics high-resolution fluorescent camera:

  • High-resolution, thermoelectrically cooled to -20°C
  • >95% peak quantum efficiency (QE)
  • Low background 1.0e-ready noise
  • 16-bit 65,356 shades of grey detection
  Light source High-output, laser-based illumination. Configured for imaging in the UV range through near-IR range with the provided filter sets.
  Objectives

Standard (Olympus™ objectives):

  • 10x (0.4 NA) X-line
  • 20x (0.45 NA) Achroplan system
  • 20x (0.7 NA) Olympus objectives
  • 40x (0.6 NA) Olympus objectives

Optional (Olympus objectives):

  • 1.25x (0.04 NA)
  • 2x (0.08 NA)
  • 4x (0.16 NA) X-line
  • 20x (0.8 NA) X-line
  • 40x (0.95 NA) X-line
  • 60x (0.90 NA)
  Image compatibility JPG, BMP, GIF, PNG, TIF, C01, DIB
Physical characteristics Dimensions 20 in. x 32 in. x 18 in. (50.8 cm x 81.3 cm x 45.7 cm)
  Weight 68 kg (150 lb)
System Data management Compatible with Thermo Scientific Store Image and Database Management Software
  Software Thermo Scientific HCS Studio 5.0 Cell Analysis Software with the cell painting bioapplication
  PC Windows 10 Professional 64-Bit PC with 32GB (4X8GB) RAM, Intel® Xeon® Processor 3.7GHz Turbo, RAID I Controller with 1.8TB Hard Drive and 256 Boot Drive.
Includes Dell 24" High Resolution Widescreen Monitor or equivalent, keyboard and mouse
  Wattage 300 W

Videos and demos

Introducing the Thermo Scientific CellInsight CX7 LZR Pro High-Content Analysis Platform

Sample data

Immunofluorescence image of human neurons

Confocal image obtained with CellInsight CX7 LZR Pro instrument. Neurons were fluorescently stained with both DAPI and MAP2 (560 nM) to counterstain the nucleus and cell body/neurites, respectively. (60X objective (0.9NA); Nipkow spinning disc at 40 micron pinhole setting)

Immunofluorescence image of HeLa spheroid

HeLa spheroids labeled with Ki67 antibody followed by Invitrogen Alexa Fluor 647 and Alexa Fluor 488 phalloidin and Hoechst 34580 stains for nuclei. Imaged on the CellInsight CX7 LZR Pro instrument using a 10x objective and 70 micron pinhole setting.

LED illumination

Confocal scan

3D confocal scan of spheroids is faster than LED illumination

 

3D, 4-color stacks were acquired on A549 spheroids using CellInsight Cx7 LZR with a 10x/0.4 NA objective. 70 µm confocal was used for all 4 colors (20 slices, 7 µm steps). Illumination was via 405, 488, 651, and 647 lasers. The scan improvement speed when using the CellInsight CX7 LZR platform over the LED instrument was 5.8x for a 1-well scan and 6.1x for a 39-well scan.

Imaging proliferating cells in spheroids using CX7 LZR Pro platform

Imaging and analyzing proliferating cells in spheroids using CX7 LZR Pro HCS instrument.

Imaging and detecting spheroid size by segmenting and quantitating EdU and CX7 LZR Pro platform

Segmentation and quantitation of EdU and spheroid size using CellInsight CX7 LZR Pro system.

Reagent selection guide

The Thermo Scientific CellInsight CX7 LZR Pro high-content instrument allows you to take advantage of the entire fluorescence spectrum to optimize your assay—and multiplex your components to ask more in-depth biological questions. Learn more about reagents for cell viability, proliferation, and function or reagents to determine cell structure. For more specific applications using assays and high-content platforms, check out Assay Selection.

For Research Use Only. Not for use in diagnostic procedures.