TaqMan Real-Time PCR Master Mixes

Applied Biosystems TaqMan master mixes have been optimized for use across a wide variety of research and discovery-stage biopharmaceutical applications. Choosing the right master mix depends on your target (RNA or DNA), application, test format, and multiplexing needs. For most qPCR research applications, we recommend using either TaqMan Fast Advanced Master Mix or TaqMan Fast Virus 1-Step Master Mixes

Explore TaqPath master mix options for clinical assay development or mid- to late-stage biopharmaceutical development

Which TaqMan Master Mix is right for you?

	TaqMan Fast Advanced Master Mix	TaqMan Fast Virus 1-Step Master Mixes
	Check price or order now	Check price or order now
Key applications	Gene expression analysis (2-step) DNA detection miRNA analysis	Gene expression analysis (1-step) RNA virus detection
Target	DNA, including cDNA	RNA
Concentration	2X	4X
Multiplexing and passive reference dye	With ROX: up to 2 targets	Available in 2 versions: With ROX: up to 3 targets No ROX: 3 or more targets
Cycling mode/run time	Fast: ~40 min Standard: ~50 min	Fast: ~60 min Standard: ~90 min
Carryover contamination control	Available in 2 versions: With UNG No UNG	No UNG
Reverse transcriptase	Purchase SuperScript IV VILO Master Mix separately and use before qPCR	Thermostable MMLV RT included in master mix
Assay recommendations	TaqMan Gene Expression assays	TaqMan Gene Expression assays
	Request a sample	Request a sample

For Research Use Only. Not for use in diagnostic procedures.