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OpenArray Technology Overview
OpenArray™ technology uses a microscope slide–sized plate with 3,072 through-holes. Each plate contains 48 subarrays, each with 64 through-holes. Each through-hole is 300 μm in diameter and 300 μm deep and is treated with hydrophilic and hydrophobic coatings. Reagents are retained in the through-holes via surface tension (see Figure). One OpenArray plate can hold as many samples as can eight traditional 384-well plates.
Custom plate formats
Custom OpenArray plates for the QuantStudio™ 12K Flex system are offered in 11 formats to provide flexible solutions for configuring your plates for gene expression, miRNA, or genotyping analysis.
| Plate format for gene expression & miRNA analysis | # of samples per plate | Minimum order (10 pack) | Total # of samples per minimum order | |
|---|---|---|---|---|
Inventoried assays only | Inventoried, made-to-order, and/or custom assays | |||
| 18 (3X) assays | 48 | 1 | 1 | 480 |
| 56 assays | 48 | 1 | 1 | 480 |
| 112 assays | 24 | 1 | 2 | 240 (inv only)/ 480 (inv/custom) |
| 168 assays | 16 | 1 | 3 | 160 (inv only)/ 480 (inv/custom) |
| 224 assays | 12 | 1 | 4 | 120 (inv only)/ 480 (inv/custom) |
Note: There are two sets of minimum order requirements for the gene expression offerings. “Inventoried” refers to OpenArray configurations where all selected assays are inventoried . “Inventoried/made to order/custom” refers to configurations that contain at least one custom or made-to-order assay.
| Plate format for genotyping analysis | # of samples per plate | Minimum order (10 pack) | Total # of samples per minimum order |
|---|---|---|---|
| 12 assays | 144 | 1 | 1,440 |
| 26 assays | 96 | 1 | 960 |
| 60 assays | 48 | 2 | 960 |
| 120 assays | 24 | 4 | 960 |
| 180 assays | 16 | 6 | 960 |
| 240 assays | 12 | 8 | 960 |
OpenArray workflow overview with the QuantStudio 12K Flex System
OpenArray technology offers a very easy-to-use format. For most applications, assays are preloaded onto plates at our state-of-the-art manufacturing facility. To run the plates, simply mix your sample with master mix, load with the automated QuantStudio 12K Flex OpenArray AccuFill™ System, cycle, and image (see Figure below).
1Select assays or panels
- Select Assays and OpenArray Plate formats for custom plates.
- Select predesigned OpenArray Pathways Panels.
- Select OpenArray Digital PCR Plates.
2Add samples & master mix
For gene expression and genotyping applications, mix cDNA or DNA samples with master mix in 384-well sample plates. For digital PCR applications, mix assays, samples, and master mix in 384-well sample plates.
3Load samples
Load sample mixes onto a QuantStudio 12K Flex OpenArray plate with the QuantStudio™ 12K Flex AccuFill™ System.
4Apply lid & fill
The QuantStudio 12K Flex OpenArray plate encased in an alloy bottom for easy handling; simply apply the adhesive lid and fill case with immersion fluid.
5Cycle, image, analyze data
Easily run up to 4 QuantStudio 12K Flex OpenArray plates for any application on the QuantStudio 12K Flex instrument in standalone mode.
OpenArray technology helps streamline real-time PCR studies that use large numbers of samples, assays, or both, because a single QuantStudio 12K Flex OpenArray plate is equivalent to running eight traditional 384-well plates. The system can run up to 4 QuantStudio 12K Flex OpenArray plates simultaneously, allowing you to process up to 1,728 genotyping samples and 2,304 samples for gene expression, typically in an 8-hour day.
Enhance your genotyping throughput by thermal cycling offline using the Dual Flat Block GeneAmp™ PCR System 9700 and process a total of more than 5,184 genotyping samples in one day. This system offers interchangeable blocks, allowing you to switch to standard multi-well plate formats as well.
Visit the OpenArray application page to learn more of each application workflow and product.
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For Research Use Only. Not for use in diagnostic procedures.