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Cell Analysis Application Notes
Articles detailing key cell biology applications
The articles listed below comprise our application notes, scientific posters, and BioProbes Journal articles collections. These resources give overviews of specific cell analysis applications and as well as experimental design details and data and results.
Resource library collections
Featured articles
Correlating internalization and potency to accelerate antibody discovery and development
In this scientific poster presented at a Keystone Symposium, we present experimental data demonstrating the use of pH-sensitive fluorophores to monitor the three primary mechanisms of endocytosis in live cells.
Optimize immunodetection with recombinant secondary antibodies—superclonal secondary antibodies for sensitive and reproducible immunoassays
Read this featured BioProbes Journal article on new recombinant secondary antibodies that are changing the world of secondary detection.
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| Type | Title | Categories |
|---|---|---|
| Application note (2011) | Apoptosis detection: Apoptosis assays for the Attune Acoustic Focusing Cytometer | Alexa Fluor, apoptosis, Attune/Attune NxT, cell structure-mitochondria, cell structure-plasma membrane, flow cytometer/flow cytometry, fluorescent dyes |
| Application note (2011) | Bacterial analysis using the Attune Acoustic Focusing Cytometer | Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, microbiology, viability |
| Application note (2011) | Detecting green fluorescent protein using the Attune Acoustic Focusing Cytometer | Attune/Attune NxT, BacMam technology, CellLight, flow cytometer/flow cytometry, fluorescent proteins, protein detection |
| Application note (2011) | Detecting red fluorescent protein using the Attune Acoustic Focusing Cytometer | Attune/Attune NxT, BacMam technology, CellLight, flow cytometer/flow cytometry, fluorescent proteins, protein detection |
| Application note (2011) | Detection of human mesenchymal stem cells using the Attune Acoustic Focusing Cytometer | Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, immunophenotyping, stem cell research |
| Application note (2011) | Detection of phosphoproteins using the Attune Acoustic Focusing Cytometer | Alexa Fluor, antibodies, Attune/Attune NxT, cell signaling, flow cytometer/flow cytometry, fluorescent dyes, protein detection |
| Application note (2011) | Immunophenotyping peripheral blood cells: A no-lyse, no-wash, no cell loss method for immunophenotyping nucleated peripheral blood cells using the Attune Acoustic Focusing Cytometer | antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, particles |
| Application note (2011) | Mouse immunophenotyping: A no-lyse, no-wash, and no cell loss method using the Attune Acoustic Focusing Cytometer | antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, particles |
| Application note (2011) | Novel detection and direct cell counting of marine, photosynthetic picoplankton by flow cytometry | Attune/Attune NxT, cell counting, flow cytometer/flow cytometry, flow cytometry sample preparation |
| Application note (2011) | Six-color mouse lymphocyte immunophenotyping using the Attune Acoustic Focusing Cytometer with red laser option | Alexa Fluor, antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, particles |
| Application note (2012) | Detecting human circulating endothelial cells using the Attune Acoustic Focusing Cytometer | Alexa Fluor, antibodies, Attune/Attune NxT, endothelial cells, flow cytometer/flow cytometry, fluorescent dyes, rare-event detection |
| Application note (2012) | Microbiological applications using the Attune Acoustic Focusing Cytometer | Attune/Attune NxT, cell health, flow cytometer/flow cytometry, fluorescent dyes, microbiology, viability |
| Application note (2012) | Monitoring neurite morphology and synapse formation in primary neurons for neurotoxicity assessments and drug screening | ArrayScan, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, neuroscience, viability |
| Application note (2013) | Multiplexed mitosis and apoptosis analysis | antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, particles |
| Application note (2013) | Optimizing the high content analysis of cell morphology using multi-dimensional imaging on the Thermo Scientific ArrayScan XTI HCA Reader: Stem cell colonies | Alexa Fluor, antibodies, ArrayScan, confocal microscopy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, immunofluorescence (IF), microplate reader, particles, stem cell research |
| Application note (2014) | Elucidating the mechanism of cell death through the use of high content analysis | apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis |
| Application note (2014) | High content cell cycle screening: Pairing FUCCI technology with Thermo Scientific HCS Studio 2.0 Software and FCS Express Image cytometry | ArrayScan, cancer, cell cycle, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader |
| Application note (2014) | Oxidative stress measurements made easy: CellROX reagents with simple workflow for high content imaging | ArrayScan, CellInsight, CellROX, high content analysis, oxidative stress |
| Application note (2014) | Using a high content analysis database as a repository for data | database management, drug discovery, high content analysis, microplate reader |
| Application note (2015) | 13-parameter immunophenotyping of human lysed whole blood with the the Attune NxT Flow Cytometer allows for the identification of B cells, NK cells, multiple T cell subsets and myeloid cells. | Alexa Fluor, antibodies, Attune/Attune NxT, cancer, flow cytometer/flow cytometry, fluorescent dyes, immune system, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot |
| Application note (2015) | Attune NxT Flow Cytometer for 6-Color immunophenotyping analysis of stained human whole blood using a no-lyse, no-wash protocol, with no compensation | Attune/Attune NxT, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Cell division and migration during wound healing visualized on the EVOS FL Auto Imaging System | cell health, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging |
| Application note (2015) | Collecting Z-stack image sequences with the EVOS FL Auto Imaging System | cell structure-all, CellLight, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, ReadyProbes |
| Application note (2015) | Comparison of cell counting using Countess II Automated Cell Counters vs. hemocytometers | automated cell counter, cell counting, Countess, hemocytometer |
| Application note (2015) | Detection of murine regulatory T cells on the Attune NxT Flow Cytometer | Attune/Attune NxT, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Fluorescent apoptosis evaluation on the Countess II FL Automated Cell Counter | apoptosis, automated cell counter, cell counting, Countess, EVOS, fluorescent dyes, viability |
| Application note (2015) | Fluorescent protein reporter gene transduction efficiency measured with the Countess II FL Automated Cell Counter | automated cell counter, cell counting, Countess, EVOS, fluorescent dyes, genetic transduction |
| Application note (2015) | Fluorescent viability assays on the Countess II FL Automated Cell Counter | automated cell counter, cell counting, Countess, EVOS, fluorescent dyes, ReadyProbes, viability |
| Application note (2015) | Guide to setting up hypoxic conditions on the EVOS FL Auto Imaging System with Onstage Incubator | EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, live-cell imaging, onstage incubator, phagocytosis |
| Application note (2015) | Image tiling and stitching using the EVOS FL Auto Imaging System | cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, Imaging sample preparation, ReadyProbes |
| Application note (2015) | Live/dead algal analysis with the Attune NxT Flow Cytometer | algae, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, viability |
| Application note (2015) | Multiparameter analysis of murine regulatory T cells and dendritic cells with the Attune NxT Flow Cytometer | Attune/Attune NxT, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | No-wash, no-lyse detection of leukocytes in human whole blood on the Attune NxT Flow Cytometer | Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, rare-event detection, violet laser-excited reagents |
| Application note (2015) | No-wash, no-lyse detection of phagocytic cells via a pHrodo BioParticles functional assay in human whole blood on the Attune NxT Flow Cytometer | antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immune system, immunophenotyping, particles, pH detection |
| Application note (2015) | Phagocytosis visualized on the EVOS FL Auto Imaging System with Onstage Incubator | CellLight, EVOS, fluorescence microscopy/fluorescence imaging, live-cell imaging, ReadyProbes |
| Application note (2015) | Quantitation of proliferating cells with the EVOS FL Auto Imaging System | antibodies, antibody labeling, ArrayScan, cell proliferation, EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, onstage incubator, phagocytosis |
| Application note (2015) | Ten-parameter immunophenotyping of human lysed whole blood with the Attune NxT Flow Cytometer allows identification of multiple T cell subsets and myeloid cells | Attune/Attune NxT, flow cytometer/flow cytometry, immunophenotyping, multicolor flow cytometry |
| Application note (2015) | Using time-lapse imaging with the EVOS FL Auto Imaging System | EVOS FL Auto Microscope, fluorescence microscopy/fluorescence imaging, hypoxia, live-cell imaging, onstage incubator |
| Application note (2016) | Blood cell counting using the Countess II FL Automated Cell Counter | automated cell counter, brightfield microscopy, cell counting, cell counting sample preparation, Countess, EVOS, fluorescent dyes, immunophenotyping, ReadyProbes |
| Application note (2016) | Detection of fluorescent proteins using the Attune NxT Flow Cytometer | Attune/Attune NxT, CellLight, flow cytometer/flow cytometry, fluorescent proteins, GFP, multicolor flow cytometry |
| Application note (2016) | Recommendations for accurate carryover measurements on the Attune NxT Flow Cytometer | Attune/Attune NxT, flow cytometer calibration, flow cytometer/flow cytometry, flow cytometry sample preparation |
| Application note (2017) | Detection of platelets in whole blood using the Attune NxT Flow Cytometer | antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescence, immunophenotyping, platelets |
| Application note (2017) | Flow cytometry analysis of dose response for apoptosis induction | apoptosis, Attune/Attune NxT, cancer, flow cytometer/flow cytometry |
| Application note (2017) | Flow cytometry analysis of transcription factor expression during differentiation of hPSC-derived cardiomyocytes | antibodies, Attune/Attune NxT, cardiomyocytes, cell differentiation, flow cytometer/flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunophenotyping, stem cell research |
| Application note (2017) | Simple and accurate monitoring of adipogenesis | adipocyte, adipogenesis, automated cell counter, cell differentiation, Countess, EVOS, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, onstage incubator, stem cell research |
| Application note (2018) | Cell-based assays to provide functional confirmation of gene knockout |
|
| Application note (2018) | Effect of phosphoinositide 3-kinase (PI3-kinase) inhibition on wound healing and cell proliferation |
|
| Application note (2018) | Automated fluorescence imaging and quantification of cell viability |
|
| Application note (2018) | CRISPR - Phenotypic fingerprint of cellular function following gene editing by high-throughput imaging acquisition and analysis | CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis, high content screening, CRISPR, gene editing, autophagy, CX5, CX7, HCS Studio |
| Application note (2019) | The importance of accurate cell counting in flow cytometry and cell sorting | flow cytometer/flow cytometry, viability, cell counting, automated cell counter, Countess |
| BioProbes articles (Issues 50–present day) | BioProbes Journal of Cell Biology Application | cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification |
| Book | Live cell imaging Published by Wiley, this Essential Knowledge Briefing provides a short, practical guide of the background and basics of live cell imaging. Content includes basic history of live cell imaging and case studies of live cell imaging applications Common problems and solutions with live cell imaging. | fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, onstage incubator |
| eBook | Live cell imaging Published by Wiley, this Essential Knowledge Briefing provides a short, practical guide of the background and basics of live cell imaging. Content includes basic history of live cell imaging and case studies of live cell imaging applications Common problems and solutions with live cell imaging. | fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, onstage incubator |
| Molecular Probes Handbook | TSA and other peroxidase-based signal amplification techniques—Section 6.2 | Alexa Fluor, antibodies, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), reagents, secondary detection, signal amplification |
| Molecular Probes Handbook | Qdot nanocrystals—Section 6.6 | avidins and streptavidins, cell tracking, fluorescence, particles, Qdot, secondary antibodies |
| Molecular Probes Handbook | Phycobiliproteins—Section 6.4 | antibody labeling, flow cytometer/flow cytometry, multicolor flow cytometry |
| Molecular Probes Handbook | Microspheres—Section 6.5 | flow cytometer calibration, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microscope calibration, particles |
| Molecular Probes Handbook | Introduction to signal amplification—Section 6.1 | antibodies, fluorescence microscopy/fluorescence imaging, secondary detection, signal amplification, western detection |
| Molecular Probes Handbook | Phosphatase-based signal amplification techniques—Section 6.3 | ELISA, signal amplification, western detection |
| Molecular Probes Handbook | Fluorescence microscopy accessories and reference standards—Section 23.1 | fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Flow cytometry reference standards—Section 23.2 | flow cytometer/flow cytometry |
| Molecular Probes Handbook | Thiol-reactive probes excited with visible light—Section 2.2 | protein labeling, fluorescent dyes |
| Molecular Probes Handbook | Thiol-reactive probes excited with ultraviolet light—Section 2.3 | protein labeling, fluorescent dyes |
| Molecular Probes Handbook | Introduction to thiol modification and detection—Section 2.1 | antibody labeling, protein labeling, sulfhydryl modification, thiol labeling, thiol modification |
| Molecular Probes Handbook | Using the Fluorescence Spectraviewer—Note 23.1 | flow cytometer/flow cytometry, fluorescence, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
| Molecular Probes Handbook | The Alexa Fluor Dye series—Note 1.1 | Alexa Fluor, fluorescent dyes |
| Molecular Probes Handbook | SYBR safe DNA gel stain—Note 8.1 | DNA gel stains, fluorescence |
| Molecular Probes Handbook | Selecting optical filters for fluorescence microscopy—Note 23.2 | fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Monitoring protein-folding processes with environment-sensitive dyes—Note 9.1 | fluorescence, protein assays and analysis, protein structure |
| Molecular Probes Handbook | Mitochondria in diseases—Note 12.2 | antibodies, cell structure-mitochondria, fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | MHC tetramer technology—Note 7.2 | antibodies, flow cytometer/flow cytometry, immunology, Major Histocompatibility Complexes, T cells |
| Molecular Probes Handbook | MAXC Computer Program for calculating Free Ca2+ concentrations—Note 19.2 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Loading and calibration of intracellular ion indicators—Note 19.1 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Lipid-mixing assays of membrane fusion—Note 13.1 | cell structure-plasma membrane, fluorescent lipids, membranes, micelles |
| Molecular Probes Handbook | Labeling small peptides with amine-reactive dyes in organic solvents—Note 9.2 | amine-reactive dyes, fluorescence, peptide labeling, protein modification |
| Molecular Probes Handbook | G Proteins and GTP analogs for binding studies—Note 17.1 | cell signaling, fluorescent dyes, receptor binding |
| Molecular Probes Handbook | Using organic fluorescent probes in combination with GFP—Note 12.1 | fluorescent dyes, fluorescent proteins |
| Molecular Probes Handbook | Fluorescent probes for two-photon microscopy—Note 1.5 | fluorescence microscopy/fluorescence imaging, two-photon microscopy |
| Molecular Probes Handbook | Fluorescent probes for photoconversion of diaminobenzidine reagents—Note 14.2 | DAB, electron microscopy |
| Molecular Probes Handbook | Fluorescence resonance energy transfer (FRET)—note 1.2 | fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Fluorescence polarization (FP)—note 1.4 | fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Fluorescence correlation spectroscopy (FCS)—Note 1.3 | fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Bacmam gene delivery and expression technology—Note 11.1 | gene expression, transient protein transfection |
| Molecular Probes Handbook | Assays of volume change, membrane fusion and membrane permeability—Note 14.3 | cell structure-plasma membrane, fluorescent lipids, membranes, micelles |
| Molecular Probes Handbook | Anti–Lucifer Yellow Dye, Anti–Alexa Fluor 405/Cascade Blue Dye and Anti–Alexa Fluor 488 Dye antibodies—Note 14.1 | antibodies, anti-dye antibodies, fluorescence, signal amplification |
| Molecular Probes Handbook | Antibody structure and classification—Note 7.1 | antibodies |
| Molecular Probes Handbook | Antibodies for detecting membrane-surface labels—Note 13.2 | antibodies, cell structure-plasma membrane, extracellular proteins |
| Molecular Probes Handbook | Reagents for modifying aldehydes and ketones—Section 3.3 | aliphatic and aromatic amines, biomolecules, hydrazines and hydroxylamines, ketones |
| Molecular Probes Handbook | Derivatization reagents for carboxylic acids and carboxamides—Section 3.4 | amine-reactive dyes, amines, carboxylic acid modification, fluorescence, hydroxylamines, labeling chemistries, protein modification and crosslinking |
| Molecular Probes Handbook | Click chemistry—Section 3.1 | azide and alkyne labeling reagents, azide–alkyne cycloaddition, bioorthogonal labeling, BrdU alternative, click reaction, Click-iT, EdU |
| Molecular Probes Handbook | Protein quantitation in solution—Section 9.2 | fluorescent dyes, protein assays and analysis, protein quantitation, Qubit, reagents |
| Molecular Probes Handbook | Introduction to protein detection—Section 9.1 | protein assays, protein detection |
| Molecular Probes Handbook | Protein detection on gels, blots and arrays—Section 9.3 | protein assays, protein detection, western blot detection |
| Molecular Probes Handbook | Probes for protein kinases, protein phosphatases and nucleotide-binding proteins—Section 17.3 | enzyme substrates, phosphatases, protein kinases |
| Molecular Probes Handbook | Probes for lipid metabolism and signaling—Section 17.4 | cell signaling, fluorescent dyes, lipid metabolism |
| Molecular Probes Handbook | Introduction to signal transduction—Section 17.1 | cell signaling, receptor binding |
| Molecular Probes Handbook | Calcium regulation—Section 17.2 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Probes for nitric oxide research—Section 18.3 | NO, nitrate, nitric oxide (NO), oxidative stress, SNAP |
| Molecular Probes Handbook | Introduction to reactive oxygen species—Section 18.1 | oxidative stress |
| Molecular Probes Handbook | Generating and detecting reactive oxygen species—Section 18.2 | oxidative stress |
| Molecular Probes Handbook | Probes for the endoplasmic reticulum and golgi apparatus—Section 12.4 | antibodies, cell structure-er, cell structure-golgi, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Molecular Probes Handbook | Probes for mitochondria—Section 12.2 | antibodies, cell structure-mitochondria, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Molecular Probes Handbook | Probes for lysosomes, peroxisomes and yeast vacuoles—Section 12.3 | antibodies, cell structure-lysosomes, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Molecular Probes Handbook | Probes for the nucleus—Section 12.5 | antibodies, cell structure-nucleus, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, membrane permeability |
| Molecular Probes Handbook | A diverse selection of organelle probes—Section 12.1 | cell structure-er, cell structure-golgi, cell structure-lysosomes, cell structure-mitochondria, cell structure-nucleus, cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging |
| Molecular Probes Handbook | Slow-response probes—Section 22.3 | fluorescence microscopy/fluorescence imaging, live-cell imaging, membrane potential dyes |
| Molecular Probes Handbook | Introduction to potentiometric probes—Section 22.1 | cell structure-plasma membrane, fast-response probes, fluorescence microscopy/fluorescence imaging, fluorometer, ion channels, live-cell imaging, membrane potential, slow-response probes |
| Molecular Probes Handbook | Fast-response probes—Section 22.2 | cell structure-plasma membrane, fast-response probes, fluorescence microscopy/fluorescence imaging, fluorometer, ion channels, live-cell imaging, membrane potential |
| Molecular Probes Handbook | Sphingolipids, steroids, lipopolysaccharides and related probes—Section 13.3 | fluorescent dyes, lipid metabolism, lipid tracking |
| Molecular Probes Handbook | Other nonpolar and amphiphilic probes—Section 13.5 | carbocyanine dyes, cell structure-plasma membrane, DiI, fluorescence microscopy/fluorescence imaging, fluorescent lipid dyes, fluorometer |
| Molecular Probes Handbook | Introduction to membrane probes—Section 13.1 | cell structure-plasma membrane, fast-response probes, fluorescence microscopy/fluorescence imaging, fluorometer, ion channels, live-cell imaging, membrane potential, slow-response probes |
| Molecular Probes Handbook | Fatty acid analogs and phospholipids—Section 13.2 | cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent fatty acid analogs, fluorescent lipid dyes, fluorometer |
| Molecular Probes Handbook | Dialkylcarbocyanine and dialkylaminostyryl probes—Section 13.4 | carbocyanine dyes, cell structure-plasma membrane, DiI, fluorescence microscopy/fluorescence imaging, fluorescent lipid dyes, fluorometer |
| Molecular Probes Handbook | Probes for neurotransmitter receptors—Section 16.2 | brain hormones, cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent dyes, receptor binding |
| Molecular Probes Handbook | Probes for ion channels and carriers—Section 16.3 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Probes for following receptor binding and phagocytosis—Section 16.1 | cell structure-plasma membrane, fluorescence microscopy/fluorescence imaging, fluorescent dyes, internalization, membrane trafficking, phagocytosis, receptor binding, vesicle |
| Molecular Probes Handbook | Probes for tubulin and other cytoskeletal proteins—Section 11.2 | cell structure-tubulin, cytoskeleton, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
| Molecular Probes Handbook | Probes for actin—Section 11.1 | cell structure-actin, cytoskeleton, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins |
| Molecular Probes Handbook | Probes useful at near-neutral pH—Section 20.2 | BCECF, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, pH detection, pH indicators, SNARF |
| Molecular Probes Handbook | Probes useful at acidic pH—Section 20.3 | flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo |
| Molecular Probes Handbook | pH indicator conjugates—Section 20.4 | flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo |
| Molecular Probes Handbook | Overview of pH indicators—Section 20.1 | flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo |
| Molecular Probes Handbook | Nucleic acid stains—Section 8.1 | cell cycle, cell structure-nucleus, cell structure-plasma membrane, cell viability, DAPI, DNA binding dyes, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, membrane permeability, RNA binding dyes, SYTO, SYTOX |
| Molecular Probes Handbook | Nucleic acid quantitation in solution—Section 8.3 | DNA binding dyes, DNA quantitation, fluorescent dyes, fluorometer, Qubit, RNA binding dyes, RNA quantitation, SYBR, SYTO, SYTOX |
| Molecular Probes Handbook | Fluorescent Na+ and K+ indicators—Section 21.1 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Detecting chloride, phosphate, nitrite and other anions—Section 21.2 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Introduction to Ca2+ measurements with fluorescent indicators—Section 19.1 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Fluorescent Mg2+ indicators—Section 19.6 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Fluorescent indicators for Zn2+ and other metal ions—Section 19.7 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Fluorescent Ca2+ indicators excited with visible light—Section 19.3 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Fluorescent Ca2+ indicators excited with UV light—Section 19.2 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Fluorescent Ca2+ indicator conjugates—Section 19.4 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Chelators, calibration buffers, ionophores and cell-loading reagents—Section 19.8 | ion channels, ion flux, ion indicators |
| Molecular Probes Handbook | Protein-based Ca2+ indicators—Section 19.5 | calcium flux, fluorescent dyes, ion indicators |
| Molecular Probes Handbook | Reagents for analysis of low molecular weight amines—Section 1.8 | amine labeling, ATTO-TAG Reagents, dialdehydes: OPA and NDA, fluorescamine, protein modification |
| Molecular Probes Handbook | Long-wavelength rhodamines, Texas Red Dyes and QSY Quenchers—Section 1.6 | amine-reactive dyes, fluorescent dyes |
| Molecular Probes Handbook | Kits for labeling proteins and nucleic acids—Section 1.2 | antibody labeling, nucleic acid labeling, protein labeling, protein modification |
| Molecular Probes Handbook | Introduction to amine modification—Section 1.1 | amine labeling, amine modification, antibody labeling, protein labeling, protein modification |
| Molecular Probes Handbook | Fluorescein, Oregon Green and Rhodamine Green Dyes—Section 1.5 | amine-reactive dyes, fluorescent dyes |
| Molecular Probes Handbook | Coumarins, pyrenes and other ultraviolet light–excitable fluorophores—Section 1.7 | UV laser |
| Molecular Probes Handbook | Bodipy Dye series—Section 1.4 | amine-reactive dyes, fluorescent dyes |
| Molecular Probes Handbook | Alexa Fluor Dyes spanning the visible and infrared spectrum—Section 1.3 | Alexa Fluor, amine-reactive dyes, fluorescent dyes |
| Molecular Probes Handbook | Tracers for membrane labeling—Section 14.4 | cell structure-plasma membrane, cell tracking, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, live-cell imaging |
| Molecular Probes Handbook | Protein conjugates—Section 14.7 | cell tracking, cell tracers, fluorescent dyes, protein conjugates |
| Molecular Probes Handbook | Polar tracers—Section 14.3 | cell tracers, fluorescent dyes, hydrazides, Lucifier Yellow CH, neuronal tracers, signal amplification |
| Molecular Probes Handbook | Membrane-permeant reactive tracers—Section 14.2 | amine-reactive cell trackers, cell tracers, CellTrace, CellTracker, CFSE, fluorescent dyes, SE, succinimidyl ester , thiol-reactive cell trackers |
| Molecular Probes Handbook | Microspheres and Qdot Nanocrystals for tracing—Section 14.6 | blood flow, FluoSpheres, neuronal tracing, particles, Qdot, Qtracker, quantum dots |
| Molecular Probes Handbook | Fluorescent and biotinylated dextrans—Section 14.5 | cell lineage tracing, cell loading, dextran conjugates, endocytosis, fluid transport, fluorescent dextrans, intercellular communications, membrane permeability, neuronal tracing |
| Molecular Probes Handbook | Choosing a tracer—Section 14.1 | cell lineage tracing, cell tracing, dextran conjugates, endocytosis, fluid transport, intercellular communications, membrane permeability, neuronal tracing, polar tracers, protein conjugates |
| Molecular Probes Handbook | Substrates for oxidases, Including Amplex Red Kits—Section 10.5 | Amplex, enzymes, enzyme substrates, oxidases |
| Molecular Probes Handbook | Substrates for microsomal dealkylases, acetyltransferases, luciferases and other enzymes—Section 10.6 | enzyme activity, enzyme substrates |
| Molecular Probes Handbook | Introduction to enzyme substrates and their reference standards—Section 10.1 | enzyme activity assays, enzyme substrates, fluorescent dyes, fluorometer, microplate reader, protein assays and analysis |
| Molecular Probes Handbook | Detecting peptidases and proteases—Section 10.4 | enzyme activity assays, enzyme substrates, fluorescent dyes, fluorometer, microplate reader, protein assays and analysis |
| Molecular Probes Handbook | Detecting glycosidases—Section 10.2 | enzyme activity assays, enzyme substrates, fluorescent dyes, fluorometer, microplate reader, protein assays and analysis |
| Molecular Probes Handbook | Detecting enzymes that metabolize phosphates and polyphosphates—Section 10.3 | enzyme activity assays, enzyme substrates, fluorescent dyes, fluorometer, microplate reader, protein assays and analysis |
| Molecular Probes Handbook | Introduction to avidin–biotin and antibody–hapten techniques—Section 4.1 | antibodies, avidins and streptavidins, fluorescence, labeling and detection, secondary antibodies, secondary detection, signal amplification |
| Molecular Probes Handbook | Biotinylation and haptenylation reagents—Section 4.2 | antibody labeling, biotinylation, protein labeling, secondary detection, signal amplification |
| Molecular Probes Handbook | Biotin and desthiobiotin conjugates—Section 4.3 | antibody labeling, biotinylation, protein labeling, secondary detection, signal amplification |
| Molecular Probes Handbook | Viability and cytotoxicity assay reagents—Section 15.2 | cell health, cell viability and function, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader |
| Molecular Probes Handbook | Viability and cytotoxicity assay kits for diverse cell types—Section 15.3 | cell health, cell viability and function, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, microplate reader |
| Molecular Probes Handbook | Probes for cell adhesion, chemotaxis, multidrug resistance and glutathione—Section 15.6 | cell adhesion assays, chemotaxis assays, glutathione determination, MDR, microsphere adhesion assays, multidrug resistance assays |
| Molecular Probes Handbook | Overview of probes for cell viability, cell proliferation and live-cell function—Section 15.1 | cell health, cell death, cell proliferation, cell viability and function |
| Molecular Probes Handbook | Assays for cell enumeration, cell proliferation and cell cycle—Section 15.4 | cell counting, cell cycle, cell proliferation |
| Molecular Probes Handbook | Assays for apoptosis and autophagy—Section 15.5 | apoptosis, autophagy, fluorescent dyes, fluorescence microscopy/fluorescence imaging, flow cytometer/flow cytometry |
| Molecular Probes Handbook | Zenon Technology: Versatile reagents for immunolabeling—Section 7.3 | antibody labeling, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, secondary detection, Zenon |
| Molecular Probes Handbook | Secondary immunoreagents—Section 7.2 | antibodies, avidins and streptavidins, secondary antibodies |
| Molecular Probes Handbook | Antibodies against expression tags—Section 7.5 | antibodies, anti-GFP, anti-GST, anti-Penta HIS, fluorescent conjugates, reporter gene antibodies |
| Molecular Probes Handbook | Lectins and other carbohydrate-binding proteins—Section 7.7 | cholera toxin, Con A, concanavalin A , fluorescent conjugates, oligosaccharides, WGA, wheat germ agglutinin |
| Molecular Probes Handbook | Introduction to antibodies, avidins and lectins—Section 7.1 | antibodies, avidins and streptavidins, fluorescence, labeling and detection, secondary antibodies, secondary detection, signal amplification |
| Molecular Probes Handbook | Avidin and streptavidin conjugates—Section 7.6 | antibodies, avidins and streptavidins, fluorescence, labeling and detection, secondary antibodies, secondary detection, signal amplification |
| Molecular Probes Handbook | Anti-dye and anti-hapten antibodies—Section 7.4 | antibodies, anti-Alexa Fluor 488, fluorescent conjugates, secondary detection, signal amplification |
| Pathway poster (2012) | TNF signaling pathway > | antibodies, cell signaling, immunoassays |
| Pathway poster (2012) | Toll-like receptor signaling pathway > | antibodies, cell signaling, immunoassays |
| Pathway poster (2012) | The human kinome > | antibodies, cell signaling, immunoassays |
| Pathway poster (2012) | Integrin signaling pathway > | antibodies, cell signaling, immunoassays |
| Pathway poster (2012) | Interferon signaling pathway > | antibodies, cell signaling, immunoassays |
| Pathway poster (2012) | MAPK signaling pathway > | antibodies, cell signaling, immunoassays |
| Pathway poster (2012) | Akt/mTOR signaling pathway > | antibodies, cell signaling, immunoassays |
| Pathway poster (2012) | Jak/Stat signaling pathway > | antibodies, cell signaling, immunoassays |
| Pierce Protein Methods | Overview of immunohistochemistry (IHC) | antibodies, fixation, immunohistochemistry (IHC) |
| Pierce Protein Methods | Immunohistochemistry methods articles | antibodies, fixation, immunohistochemistry (IHC) |
| Pierce Protein Methods | Overview of protein labeling | antibodies, antibody labeling, cell signaling, fluorescent dyes, fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), protein labeling |
| Protocol | XTT Cell Viability Assay | cell viability, microplate |
| Protocol | Vybrant MTT Cell Proliferation Assay Kit | cell viability, microplate |
| Protocol | Viability staining protocol for flow cytometry | cell viability, flow cytometry |
| Protocol | TO-PRO-3 Stain | cell structure, imaging |
| Protocol | SYTOX Green Nucleic Acid Stain | cell structure, imaging |
| Protocol | SYTOX Dead Cell Stains | cell viability, flow cytometry |
| Protocol | SYTO 82 Nuclear Stain | cell structure, imaging |
| Protocol | SYTO 59 Nuclear Stain | cell structure, imaging |
| Protocol | Staining intracellular antigens for flow cytometry | flow cytometry, general ab staining |
| Protocol | Staining cell surface targets for flow cytometry | flow cytometry, general ab staining |
| Protocol | Red blood cell lysis protocols using eBioscience lysis buffers | cell proliferation, flow cytometry |
| Protocol | ReadyProbes Cell Viability Imaging Kit, Blue/Red | cell viability, imaging |
| Protocol | ReadyProbes Cell Viability Imaging Kit, Blue/Green | cell viability, imaging |
| Protocol | PrestoBlue Cell Viability Reagent for microplates | cell viability, microplate |
| Protocol | PrestoBlue and CyQUANT Direct Confirmation Assay | cell viability, microplate |
| Protocol | Pharmacological induction of apoptosis with camptothecin | cell vitality, flow cytometry, high content analysis, imaging, microplate |
| Protocol | OneComp and UltraComp Compensation Beads protocols for flow cytometry | experimental standards, flow cytometry |
| Protocol | NucRed Live 647 ReadyProbes Reagent | cell structure, imaging |
| Protocol | NucRed Dead 647 ReadyProbes Reagent for viability | cell viability, imaging |
| Protocol | NucRed Dead 647 ReadyProbes Reagent for fixed cells | cell structure, imaging |
| Protocol | NucGreen Dead 488 ReadyProbes Reagent for viability | cell viability, imaging |
| Protocol | NucGreen Dead 488 ReadyProbes Reagent for fixed cells | cell structure, imaging |
| Protocol | NucBlue Live ReadyProbes Reagent | cell structure, imaging |
| Protocol | NucBlue Fixed Cell ReadyProbes Reagent | cell structure, imaging |
| Protocol | LIVE/DEAD Yeast Viability Kit | cell viability, imaging |
| Protocol | LIVE/DEAD Violet Viability/Vitality Kit | cell viability and cell vitality, flow cytometry |
| Protocol | LIVE/DEAD Viability/Cytotoxicity Kit for mammalian cells | cell viability, imaging |
| Protocol | LIVE/DEAD Sperm Viability Kit for flow cytometry | cell viability, flow cytometry |
| Protocol | LIVE/DEAD Sperm Viability Kit for imaging | cell viability, imaging |
| Protocol | LIVE/DEAD Fixable Dead Cell Stains | cell viability, flow cytometry |
| Protocol | LIVE/DEAD Cell Imaging Kit (488/570) | cell viability, imaging |
| Protocol | LIVE/DEAD BacLight Bacterial Viability Kit | cell viability, imaging |
| Protocol | LIVE BacLight Bacterial Gram Stain Kit | cell viability, imaging |
| Protocol | Immunofluorescent staining of intracellular antigens on cultured cells | fluorescence microscopy/fluorescence imaging, general ab staining, imaging, immunofluorescence (IF) |
| Protocol | Image-iT DEAD Green Kit | cell viability, imaging |
| Protocol | Immunohistochemical staining of formalin-fixed paraffin-embedded tissues | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC frozen tissue—Indirect method (purified) | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC frozen tissue—Indirect method (biotin) | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC frozen tissue—Direct method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE Tissue Trypsin Digestion—Direct Method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE tissue trypsin digestion antigen retrieval—Indirect method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE tissue low pH antigen retrieval—Indirect method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE tissue low pH antigen retrieval—Direct method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE tissue high pH antigen retrieval—Indirect method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | IHC FFPE tissue high pH antigen retrieval—Direct method | general ab staining, imaging, immunohistochemistry (IHC) |
| Protocol | ICC unfixed cells—Direct method | general ab staining, imaging |
| Protocol | ICC methanol fixed cells—Indirect method | general ab staining, imaging |
| Protocol | ICC methanol fixed cells—Direct method | general ab staining, imaging |
| Protocol | ICC formaldehyde fixed, permeabilized cells—Indirect method | general ab staining, imaging |
| Protocol | ICC formaldehyde fixed, permeabilized cells—Direct method | general ab staining, imaging |
| Protocol | ICC formaldehyde fixed cells—Indirect method | general ab staining, imaging |
| Protocol | ICC formaldehyde fixed cells—Direct method | general ab staining, imaging |
| Protocol | Hoechst 33342 for imaging | cell structure, imaging |
| Protocol | Hoechst 33342 for HCA instruments | cell structure, high content analysis |
| Protocol | HCS NuclearMask Red Stain | cell structure, high content analysis |
| Protocol | HCS NuclearMask Deep Red Stain | cell structure, high content analysis |
| Protocol | HCS NuclearMask Blue Stain | cell structure, high content analysis |
| Protocol | HCS Mitochondrial Health Kit | cell viability, high content analysis |
| Protocol | HCS LIVE/DEAD Green Kit using Hoechst 33342 | cell viability, high content analysis |
| Protocol | HCS LIVE/DEAD Green Kit using HCS NuclearMask Deep Red | cell viability, high content analysis |
| Protocol | HCS CellMask Stains | cell structure, high content analysis |
| Protocol | Staining cells with eFluor proliferation dyes for flow cytometry | cell proliferation, flow cytometry |
| Protocol | DAPI for fluorescence imaging | cell structure, imaging |
| Protocol | DAPI for HCA instruments | cell structure, high content analysis |
| Protocol | CyQUANT Direct Microplate Reagent for cell viability | cell viability, microplate |
| Protocol | Colorimetric FFPE—trypsin digestion | colorimetric staining, general ab staining, imaging |
| Protocol | Colorimetric FFPE—Low pH antigen retrieval | colorimetric staining, general ab staining, imaging |
| Protocol | Colorimetric FFPE—High pH antigen retrieval | colorimetric staining, general ab staining, imaging |
| Protocol | Click-iT Plus EdU imaging kits | cell proliferation, imaging |
| Protocol | Click-iT EdU imaging kits | cell proliferation, imaging |
| Protocol | Click-iT EdU HCS assays | cell proliferation, imaging |
| Protocol | CellTrace Violet Cell Proliferation Kit | cell proliferation, flow cytometry |
| Protocol | CellTrace Far Red Cell Proliferation Kit | cell proliferation, flow cytometry |
| Protocol | CellTrace CFSE Cell Proliferation Kit | cell proliferation, flow cytometry |
| Protocol | Cell preparation for flow cytometry protocols | cell proliferation, flow cytometry |
| Protocol | BrdU staining protocol for flow cytometry | cell proliferation, flow cytometry |
| Protocol | BrdU labeling and detection | cell proliferation, imaging |
| Protocol | Annexin V staining protocol for flow cytometry | cell vitality, flow cytometry |
| Protocol | ActinRed 555 ReadyProbes Reagent | cell structure, imaging |
| Protocol | ActinGreen 488 ReadyProbes Reagent | cell structure, imaging |
| Scientific poster (2006) | LipidTOX dyes for adipocyte staining in routine imaging applications > | cell differentiation, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, stem cell research, viability |
| Scientific poster (2006) | Cell cycle analysis using microplate cytometry: A comparison of laser and dye combinations | Acumen Explorer, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader |
| Scientific poster (2006) | New fluorescent reagents for the imaging-based assays for the analysis of drug-induced perturbations of cellular lipid metabolism | ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, lipid detection, lipids, microplate reader, viability |
| Scientific poster (2006) | Utility of 405 nm-excitable dyes in high content screening using an Acumen Explorer Microplate Cytometer | Acumen Explorer, apoptosis, cell cycle, cell health, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, violet laser-excited reagents |
| Scientific poster (2006) | Novel violet-excited reagents for detection of viability and vitality | Alexa Fluor, cell health, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, viability, violet laser-excited reagents |
| Scientific poster (2007) | Evaluation of click chemistry-based alternative to BrdU antibody labeling in tissue and cultured cells using fluorescence microscopy and flow cytometry | antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, microplate reader, nucleic acid labeling |
| Scientific poster (2007) | A new approach for the detection of intracellular glutathione by fluorescence microscopy and flow cytometry | ArrayScan, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, live-cell imaging, microplate reader, viability, violet laser-excited reagents |
| Scientific poster (2007) | Click chemistry-based detection of S-phase adherent cells using automated microscopy and image analysis | Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader |
| Scientific poster (2007) | Click catalyzed nucleic acid labeling as a novel replacement for BrdU antibody-based cell proliferation assay | Alexa Fluor, antibodies, antibody labeling, cell cycle, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling |
| Scientific poster (2007) | BacMam gene delivery of targeted fluorescent proteins, Organelle Lights for HCS | BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, live-cell imaging, nanocrystals, Qdot |
| Scientific poster (2008) | Living molecular probes for the next generation | BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, ion flux/ion channels, ion indicators, live-cell imaging |
| Scientific poster (2008) | Quantum dot (Qdot) labeling of gene expression in fresh frozen brain tissue using high-throughput in situ hybridization | in situ hybridization, brightfield microscopy, confocal microscopy,fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, nanocrystals, neuroscience, Qdot, tissue |
| Scientific poster (2008) | Characterization of DNA content, cyclin B1 and phosphorylated histone H3 with direct S-phase using EDU incorporation in multiparameter testing of cell lines with cell cycle blocking agents | Alexa Fluor, antibodies, apoptosis, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis |
| Scientific poster (2008) | Zenon labeling technology facilitates detection of intra-cellular phosphoprotein determinants | Alexa Fluor, antibodies, cell signaling, flow cytometer/flow cytometry, fluorescent dyes, protein labeling, rare cell detection, Zenon |
| Scientific poster (2008) | Cell-based potassium ion channel screening with the FluxOR Assay | BacMam technology, cell counting, Countess, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader |
| Scientific poster (2008) | Novel Click-IT TUNEL Assay for detection of cell death | Alexa Fluor, antibodies, apoptosis, Click-iT, cytotoxicity, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, immunocytochemistry (ICC), viability |
| Scientific poster (2008) | Effects of the thymidine analogues EdU and BrdU on cell viability and cycle progression | Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, viability |
| Scientific poster (2008) | Detection of S-phase cell cycle progression using 5-ethynyl-2'-deoxyuridine incorporation with click chemistry | Alexa Fluor, antibodies, ArrayScan, cell cycle, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling, nucleic acid quantitation |
| Scientific poster (2008) | Improving downstream results with automated cell counting | automated cell counter, BacMam technology, cell counting, cell expansion, cell isolation, Countess, fluorescent dyes, hemocytometer, ion flux/ion channels, microplate reader |
| Scientific poster (2008) | FluxOR, a universal potassium channel assay: Screening BacMam-delivered and stably expressed targets | BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader |
| Scientific poster (2008) | ThiolTracker Violet dye, a new 405 nm excitable fluorescent probe for detecting intracellular thiols in imaging-based assays | ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, glutathione, high content analysis, microplate reader, protein labeling, violet laser-excited reagents |
| Scientific poster (2008) | Modified tumor cell protein expression and modification under physiological oxygen | Alexa Fluor, cancer, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, physiological oxygen level, protein labeling |
| Scientific poster (2008) | A new 405 nm excitable fluorescent probe of intracellular thiols for imaging and flow cytometric assays | ArrayScan, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, high content analysis, microplate reader, thiol modification, violet laser-excited reagents |
| Scientific poster (2008) | Targeting serum glycoprotein cancer biomarkers with click chemistry | cancer, Click-iT, fluorescent dyes, gel electrophoresis, protein labeling |
| Scientific poster (2008) | Detection of DNA synthesis by automated microscopy and image analysis: Comparison of BRDU method and a new click chemistry-based EDU method | Alexa Fluor, antibodies, ArrayScan, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling |
| Scientific poster (2009) | Cell-based assays for predictive hepatotoxicity measurements using high content imaging | Alexa Fluor, ArrayScan, cell structure-plasma membrane, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, glutathione, high content analysis, microplate reader, nucleic acid quantitation, viability |
| Scientific poster (2009) | A non-radioactive method for detecting nascent protein synthesis by high-content imaging utilizing click chemistry | Alexa Fluor, apoptosis, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, protein detection |
| Scientific poster (2009) | A comparative study of 25 different CD4 conjugates | Alexa Fluor, antibodies, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot |
| Scientific poster (2009) | Monitoring mitotic cells and DNA content by automated imaging and analysis | Alexa Fluor, cell cycle, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability |
| Scientific poster (2009) | Dual pulse labeling of S-phase population using click chemistry to measure changes in cell proliferation | Alexa Fluor, cancer, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes |
| Scientific poster (2009) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry | Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, nucleic acid quantitation, stem cell research, violet laser-excited reagents |
| Scientific poster (2009) | Dual pulse labeling using a new thymidine analog 5-ethynyl-2'-deoxyuridine (EdU) to detect alterations in S-phase progression by fluorescence microscopy and flow cytometry | Alexa Fluor, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Scientific poster (2009) | High content imaging of endocytosis & phagocytosis using pHrodo conjugates | Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability |
| Scientific poster (2009) | Click chemistry-based detection of nascent RNA synthesis using high content imaging and fluorescence microscopy | Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, nucleic acid labeling, viability |
| Scientific poster (2009) | A simple, fast and quantitative single-step dead-cell indicator for flow cytometry | apoptosis, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, nucleic acid quantitation, viability |
| Scientific poster (2009) | High content analysis of cytotoxicity by laser scanning fluorescence microplate cytometry | Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, viability |
| Scientific poster (2009) | Accuracy and precision comparison of the hemocytometer and automated cell counting methods | automated cell counter, cell counting, Countess, hemocytometer, particles, viability |
| Scientific poster (2009) | A novel xeno-free and serum-free culture medium for the expansion of human mesenchymal stem cells | cell culture/expansion, flow cytometer/flow cytometry, fluorescent dyes, gene expression, stem cell research |
| Scientific poster (2009) | Dead cell stains in flow cytometry: A comprehensive analysis | apoptosis, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, viability |
| Scientific poster (2009) | Non-radioactive targeting of multiple classes of protein post-translational modifications (PTMs) with click chemistry | cell signaling, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling, western blotting |
| Scientific poster (2009) | Qdot nanocrystal conjugates in multispectral flow cytometry | Alexa Fluor, antibodies, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot, violet laser-excited reagents |
| Scientific poster (2009) | High content imaging and analysis of mitotoxicity and cytotoxicity in fixed cells | cell structure-mitochondria, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, viability |
| Scientific poster (2009) | Quantitative analysis of genotoxicity and cytotoxicity to DNA damaging agents using high-content imaging | Alexa Fluor, antibodies, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid labeling, nucleic acid quantitation, viability |
| Scientific poster (2009) | Metabolic labeling and click chemistry detection of stem cell markers | Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein labeling, stem cell research |
| Scientific poster (2009) | Cell-based potassium ion channel screening with BacMam using the FluxOR Assay | BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader |
| Scientific poster (2009) | Click chemistry-based assays for fluorescence microscopy and high content imaging | Alexa Fluor, apoptosis, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, nucleic acid detection, protein detection, viability |
| Scientific poster (2010) | Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry | apoptosis, autophagy, caspase substrates, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress |
| Scientific poster (2010) | Acoustic flow cytometry for multicolor immunophenotyping analysis | antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immune system, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot, violet laser-excited reagents |
| Scientific poster (2010) | Acoustic focusing cytometry: Sensitivity and throughput | apoptosis, Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, phagocytosis |
| Scientific poster (2010) | High content imaging of autophagy | antibodies, ArrayScan, autophagy, BacMam technology, cell signaling, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader |
| Scientific poster (2010) | Analysis of proliferation dynamics in primary and immortalized human cells | Alexa Fluor, cancer, cell proliferation, CellTrace, circulatory system, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescent proteins, nucleic acid quantitation |
| Scientific poster (2010) | Oxidative stress-induced protein modification: Application of clickable linoleic acid analogs | Alexa Fluor, ArrayScan, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, high content analysis, microplate reader, oxidative stress, protein detection, protein enrichment |
| Scientific poster (2010) | Antibody- and fluorescent protein-based approaches to measuring autophagy in mammalian cells by fluorescence microscopy | Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging |
| Scientific poster (2010) | Proliferative and phenotypic characterization of human mesenchymal stem cells by flow cytometry and imaging | Alexa Fluor, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, immunofluorescence (IF), immunophenotyping, stem cell research, violet laser-excited reagents |
| Scientific poster (2010) | Novel tools to enable high resolution gene expression by investigating the nascent transcriptome | Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, high content analysis, magnetic beads, nucleic acid labeling, PCR/qPCR, viability |
| Scientific poster (2010) | Click chemistry-based enrichment and identification of nascent and post-translationally modified proteins | cells, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling |
| Scientific poster (2010) | Autophagosomal accumulation perturbs Golgi structure without affecting other organelles: Implications for autophagosome biogenesis | Alexa Fluor, antibodies, autophagy, BacMam technology, cell structure-golgi, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging |
| Scientific poster (2010) | A new generation of cellular imaging probes for neurosciences research | autophagy, BacMam technology, CellLight, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels, ion indicators |
| Scientific poster (2010) | Use of surface-modified microspheres for setting compensation in multicolor flow cytometry | antibodies, flow cytometer/flow cytometry, flow cytometry compensation, fluorescent dyes, multicolor flow cytometry, particles, violet laser-excited reagents |
| Scientific poster (2010) | ABfinity antibodies: A novel approach to antibody development | Alexa Fluor, antibodies, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gene expression, immunocytochemistry (ICC), western blotting |
| Scientific poster (2010) | NA-Fluor Influenza Neuraminidase Assay Kit: Standardization of the classic MUNANA-based neuraminidase activity assay for monitoring antiviral resistance in influenza | drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, influenza, microbiology, microplate reader |
| Scientific poster (2010) | Simultaneous analysis of cell death mechanisms and oxidative stress using live cell fluorescence microscopy | apoptosis, ArrayScan, BacMam technology, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader, oxidative stress |
| Scientific poster (2010) | A comparison of three techniques to induce efficient ex vivo T cell expansion | Alexa Fluor, antibodies, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, immune system, magnetic beads, multicolor flow cytometry, viability |
| Scientific poster (2010) | New fluorescent probes for live-cell imaging | autophagy, cell cycle, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, pH detection, phagocytosis |
| Scientific poster (2010) | The NA-XTD Influenza Neuraminidase Assay Kit: Extended-glow chemiluminescence assay system for highly sensitive influenza neuraminidase assays | chemiluminescence, drug discovery,fluorescence microscopy/fluorescence imaging, high content analysis, influenza, microbiology, microplate reader |
| Scientific poster (2010) | Functional characterization of a novel fluorescent dye for proliferation analysis | Alexa Fluor, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, violet laser-excited reagents |
| Scientific poster (2010) | Non-cytotoxic near-IR DNA stain for cell cycle analysis in living cells | cell cycle, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, live-cell imaging, nucleic acid labeling, viability |
| Scientific poster (2010) | Click chemistry enrichment of cell surface glycoproteins | cells, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling |
| Scientific poster (2010) | Identification and characterization of O-GlcNAc modification of galectin-1 in mesenchymal stem cells using click chemistry | Alexa Fluor, BacMam technology, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, immunocytochemistry (ICC), protein enrichment, protein labeling, western blotting |
| Scientific poster (2010) | BacMam gene expression for functional and imaging applications | BacMam technology, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels |
| Scientific poster (2010) | Rare event detection using acoustic cytometry | Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, rare-event detection |
| Scientific poster (2010) | Click chemistry-based universal enrichment columns for global identification of azide-modified proteins | Alexa Fluor, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling |
| Scientific poster (2010) | Dual pulse labeling using EdU and BrdU to monitor alterations in cellular proliferation by fluorescence microscopy and flow cytometry | Alexa Fluor, cell cycle, cell proliferation, Click-iT, drug discovery, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes |
| Scientific poster (2010) | A universal click chemistry-based enrichment column for the identification of multiple subclasses of PTM-modified proteins and newly synthesized proteins | Alexa Fluor, Click-iT, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, gel electrophoresis, protein detection, protein enrichment, protein labeling |
| Scientific poster (2011) | Detection of human circulating endothelial cells and mouse plasmacytoid dendritic cells | Attune/Attune NxT, endothelial cells, flow cytometer/flow cytometry, fluorescent dyes, rare-event detection |
| Scientific poster (2011) | Acoustic flow cytometry for multicolor immunophenotyping analysis | antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot, violet laser-excited reagents |
| Scientific poster (2011) | No-lyse no-wash immunophenotyping using acoustic cytometry | Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, nanocrystals, Qdot |
| Scientific poster (2011) | Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health | ArrayScan, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress, viability |
| Scientific poster (2011) | Acoustic cytometry for rare event detection of PNH cells | Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, rare-event detection |
| Scientific poster (2011) | Characterization of human limbal stem cells using acoustic focusing cytometry | Attune/Attune NxT, cell culture/expansion, flow cytometer/flow cytometry, fluorescent dyes, rare-event detection, stem cell research, tissue, violet laser-excited reagents |
| Scientific poster (2011) | Phospho‐protein detection using acoustic cytometry | Alexa Fluor, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, protein detection |
| Scientific poster (2011) | Acoustic cytometric identification and initial characterization of limbal stem cells isolated from human cornea | Attune/Attune NxT, cell culture/expansion, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, PCR/qPCR, stem cell research, tissue, violet laser-excited reagents |
| Scientific poster (2011) | Simultaneous analysis of cell death mechanisms and oxidative stress using high content imaging | Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2011) | Primary human cells from Life Technologies: Offerings, scale and applications in HCS and HTS formats | Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, cell signaling, Click-iT, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis |
| Scientific poster (2011) | Validation of high content imaging and analysis assays for cell health and cytotoxicity | antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, cell health, cell proliferation, CellLight, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, pH detection |
| Scientific poster (2011) | The next step in the analysis of lymphocyte proliferation | Alexa Fluor, antibodies, Attune/Attune NxT, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, live-cell imaging, violet laser-excited reagents |
| Scientific poster (2011) | Rare event analysis: Detection of mouse plasmacytoid dendritic cells and human circulating endothelial cells | Alexa Fluor, Attune/Attune NxT, endothelial cells, flow cytometer/flow cytometry, fluorescent dyes, rare-event detection |
| Scientific poster (2011) | Flash & Glow 1,2-dioxetane chemiluminescent substrate formulations for clinical research platforms | antibodies, chemiluminescence, ELISA, enzyme activity detection, magnetic beads, microplate reader, substrate comparison |
| Scientific poster (2011) | The next generation of cell-based imaging assays for apoptosis, autophagy and oxidative stress from Molecular Probes | Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2011) | Site-specific click chemistry-mediated labeling of antibody glycans using metabolic and enzymatic approaches | Alexa Fluor, antibodies, antibody labeling, Click-iT, fluorescent dyes, western blotting |
| Scientific poster (2011) | NA-Fluor and NA-XTD Influenza Neuraminidase Assays for neuraminidase quantitation and inhibition assays | chemiluminescence, drug discovery,fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, influenza, microbiology, microplate reader |
| Scientific poster (2011) | Next-generation chemiluminescent secreted placental alkaline phosphatase (SEAP) reporter gene assay | chemiluminescence, enzymes, gene expression, luminometer, media, reagents, substrate comparison |
| Scientific poster (2011) | Application of acoustic cytometry in microbiology | Attune/Attune NxT, cell concentration, cell health, flow cytometer/flow cytometry, fluorescent dyes, microbiology, viability |
| Scientific poster (2012) | Studying malaria using the Attune flow cytometer | Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, malaria, parasite identification |
| Scientific poster (2012) | Ultrasmall and beyond: Nuclear holopoloid genome sizing of carnivorous plant species by acoustic focusing cytometry | Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, genome sizing, plants |
| Scientific poster (2012) | Rapid, in vitro T lymphocyte cell tracing by acoustic focusing cytometry | Attune/Attune NxT, cell proliferation, CellTrace, flow cytometer/flow cytometry, fluorescent dyes, magnetic beads, stem cell research |
| Scientific poster (2012) | High sensitivity applications for the Applied Biosystems Attune | Attune/Attune NxT, caspase substrates, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, viability |
| Scientific poster (2012) | Characterization of human limbal stem cells | Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, rare-event detection, stem cell research, tissue, violet laser-excited reagents |
| Scientific poster (2012) | A novel no-wash, no-lyse method for residual leukocyte enumeration using acoustic focusing flow cytometry and a cell-membrane permeant DNA dye | Attune/Attune NxT, cell counting, flow cytometer/flow cytometry, flow cytometry sample preparation, fluorescent dyes, immunophenotyping, rare-event detection, violet laser-excited reagents |
| Scientific poster (2012) | Cell-based analysis of oxidative stress, lipid peroxidation and lipid peroxidation-derived protein modifications using fluorescence microscopy | flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, high content analysis, lipid peroxidation, microplate reader, oxidative stress, viability |
| Scientific poster (2013) | Pacific Green dye: A new fluorophore for violet laser excitation | Alexa Fluor, antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, fluorescent dyes, immunophenotyping, multicolor flow cytometry, nanocrystals, Qdot, viability, violet laser-excited reagents |
| Scientific poster (2013) | Monitor caspase 3/7 activity without cell fixation: A novel apoptosis reagent from Molecular Probes | Alexa Fluor, apoptosis, Attune/Attune NxT, caspase substrates, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry |
| Scientific poster (2013) | Cell-based measurements of oxidative stress with a new near infrared emitting probe for reactive oxygen species: Applications for multiplexed evaluation of cell health | apoptosis, ArrayScan, caspase substrates, cell health, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2013) | Reactive oxygen probes—A broad range of colors with easier labeling: Novel CellROX reagents from Molecular Probes | cells, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, oxidative stress, viability |
| Scientific poster (2013) | Improved click chemistry demonstrating EdU cell proliferation with GFP-expressing cells and R-PE-based immunophenotyping | Alexa Fluor, Attune/Attune NxT, cell cycle, cell proliferation, Click-iT, flow cytometer/flow cytometry, fluorescent dyes, fluorescent proteins, immunophenotyping |
| Scientific poster (2013) | Reactive oxygen probes—A broad range of colors with easier labeling and compatibility with fixation: Novel CellROX reagents from Molecular Probes | cells, flow cytometer/flow cytometry, fluorescent dyes, multicolor flow cytometry, oxidative stress |
| Scientific poster (2013) | Cells coming to life: Tools for visualizing immune response | Attune/Attune NxT, endocytosis/phagocytosis, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, immunophenotyping, live-cell imaging, pH detection |
| Scientific poster (2013) | Illuminating endocytosis with targeted pH-sensitive fluorescent compounds | Alexa Fluor, ArrayScan, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, high content analysis, pH detection, phagocytosis, viability |
| Scientific poster (2014) | Flow cytometry multiplexing used to analyze metabolic activity and assess pharmaceutical compound toxicity as a high-throughput screening tool | Attune/Attune NxT, drug discovery, flow cytometer/flow cytometry |
| Scientific poster (2014) | Copper-safe Click-iT Plus EdU proliferation assay: Improved compatibility with simultaneous phycobiliprotein and fluorescent protein detection | Attune/Attune NxT, cell proliferation, Click-iT, flow cytometer/flow cytometry, multicolor flow cytometry |
| Scientific poster (2014) | New fluorescent probes and sensors for visualizing endocytosis, lysosomal dynamics and autophagy | autophagy, cell structure-lysosomes, endocytosis/phagocytosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader |
| Scientific poster (2014) | Click-iT EdU Plus for flow cytometry: enhanced compatibility for multiplexing | Attune/Attune NxT, cell proliferation, Click-iT, flow cytometer/flow cytometry, multicolor flow cytometry |
| Scientific poster (2015) | A no lyse, no-wash approach to characterizing phagocyte phenotype and function in whole human blood on the Attune NxT Flow Cytometer | Attune/Attune NxT, cell cycle, flow cytometer/flow cytometry, immunophenotyping, no lyse, phagocytosis, whole blood |
| Scientific poster (2015) | Colorimetric dual labeled EdU/BrdU technology demonstrates contextual information and dynamics of proliferation in tissue | apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC) |
| Scientific poster (2015) | Visualizing the life and death of cells: novel probes and platforms | apoptosis, ArrayScan, autophagy, cell death, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis |
| Scientific poster (2015) | Intracellular detection of hypoxia in live cells | EVOS, fluorescence microscopy/fluorescence imaging, hypoxia, EVOS, onstage incubator |
| Scientific poster (2016) | High content screening in MCF7 and MDA-MB231 cells show differential responses depending on oxygen levels and mechanistic readout for viability | cell health, cell viability and function, drug discovery, high content analysis, high throughput screening |
| Scientific poster (2016) | Correlating internalization and potency to accelerate antibody discovery and development | brightfield microscopy, colorimetric, endocytosis and phagocytosis, EVOS,fluorescence microscopy/fluorescence imaging, immunofluorescence (IF), immunohistochemistry (IHC) |
| Scientific poster (2016) | An objective test to qualify plate samplers for complex flow cytometric assays | Attune/Attune NxT, autosampler, flow cytometer/flow cytometry |
| Scientific poster (2016) | Cell mediated cytotoxicity in "untouched" whole blood | Attune/Attune NxT, cell concentration, cell cycle, flow cytometer/flow cytometry, immunophenotyping, no lyse, whole blood |
| Scientific poster (2016) | A novel non-cytotoxic fluorescent dye for cell proliferation analysis in flow cytometry | Attune/Attune NxT, cell health, cell proliferation, CellTrace, EVOS, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging |
| Scientific poster (2016) | No-lyse no-wash new strategy for CD34+ for absolute cell counting without beads | antibodies, Attune/Attune NxT, flow cytometer/flow cytometry, flow cytometry compensation, immunophenotyping, multicolor flow cytometry, particles |
| Scientific poster (2016) | Novel in vitro method for screening inhibitors of protein translation | cancer, drug discovery, high throughput screening, microplate reader, protein translation |
| Scientific poster (2016) | Incorporating histological analysis into existing high content imaging | antibodies, ArrayScan, brightfield microscopy, fluorescence microscopy/fluorescence imaging, high content analysis, immunohistochemistry (IHC) |
| Scientific poster (2016) | A microsphere-based universal compensation approach | Attune/Attune NxT, Flow cytometer calibration, flow cytometer/flow cytometry, flow cytometry sample preparation, immunophenotyping, multicolor flow cytometry |
| Scientific poster (2016) | Click colorimetric EdU proliferation and TUNEL: Click chemistry for brightfield microscopy | apoptosis, brightfield microscopy, cell proliferation, Click-iT, brightfield microscopy, fluorescence microscopy/fluorescence imaging, immunohistochemistry (IHC) |
| Scientific poster (2017) | Matching refractive index of mounting media improves axial resolution and image quality in 3D biological samples | antifades, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, fluorescent proteins, imaging sample preparation, mountant, onstage incubator, photobleaching |
| Scientific poster (2017) | Hypoxia measurements in live and fixed cells using fluorescence microscopy and high-content imaging | EVOS, fluorescence microscopy/fluorescence imaging, high content analysis, hypoxia, live-cell imaging, onstage incubator |
| Scientific poster (2018) | Visualizing microtubule dynamics in live cells | microscopy/fluorescence imaging, high content analysis, microplate reader, live-cell imaging, CellInsight, EVOS, fluorescent proteins, neuroscience |
| Scientific poster (2018) | Evaluation of Cellular Senescence through Fluorescence Characterization | microscopy/fluorescence imaging, high content analysis, flow cytometer/flow cytometry, CellInsight, EVOS, fluorescent proteins, neuroscience, Click-iT |
| Scientific poster (2018) | Evaluating antibody-mediated cellular cytotoxicity and potency of antibody-drug conjugates within three-dimensional tumor models | evaluating-antibody-cellular-cytotoxicity-potency-conjugates-tumor-models-poster.pdf |
| White paper (2015) | Accuracy and precision with the Countess II FL Automated Cell Counter | automated cell counter, brightfield microscopy, cell counting, Countess |
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.
For Research Use Only. Not for use in diagnostic procedures.