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Autophagy Information
This section features educational webinars, application notes, articles and more for studying the process of autophagy.
Autophagy features
Scientific poster
New fluorescent probes and sensors for visualizing endocytosis, lysosomal dynamics and autophagy
Here we describe recently developed tools to study mechanisms of cargo transport to the lysosome: endocytosis and autophagy, as well as novel tools to examine lysosomal structure and function.
On-demand webinar
Basic techniques in autophagy research
This webinar will introduce you to a series of analytical tools and techniques to help you identify and interrogate key features of autophagy.
Autophagy learning resources
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| Type | Title | Categories |
|---|---|---|
| Application note (2014) | Elucidating the mechanism of cell death through the use of high content analysis | apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis |
| BioProbes article (2010) | Putting autophagy into context | autophagy, CellInsight fluorescence microscopy/fluorescence imaging, high content analysis, oxidative stress |
| BioProbes article (2010) | Probes for visualizing secretory transport and autophagy | autophagy, cell structure, cell structure-ER, cell structure-golgi, cell structure-mitochondria, CellLight, fluorescence microscopy/fluorescence imaging, high content analysis |
| BioProbes article (2010) | Cells under stress—Fluorescence-based tools for investigating autophagy | apoptosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis |
| BioProbes article (2013) | Autophagy made visible—Advanced fluorescent probes for imaging distinct processes in the autophagy pathway | autophagy, cell structure, cell structure-lysosomes, CellLight, fluorescence microscopy/fluorescence imaging, high content analysis |
| BioProbes article (2015) | High-content analysis applied to autophagy—Automating cell imaging with Thermo Scientific HCA systems | ArrayScan, autophagy, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis |
| BioProbes article (2017) | Appetite for destruction—Studying macroautophagy with Premo fluorescent protein–based sensors | autophagy, fixed-cell imaging, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, immunocytochemistry (ICC), immunofluorescence (IF), live-cell imaging |
| Molecular Probes Handbook | Assays for apoptosis and autophagy—Section 15.5 | apoptosis, autophagy, fluorescent dyes, fluorescence microscopy/fluorescence imaging, flow cytometer/flow cytometry |
| Scientific poster (2010) | Simultaneous analysis of cell death mechanisms and oxidative stress using Molecular Probes next generation reagents for imaging and flow cytometry | apoptosis, autophagy, caspase substrates, flow cytometry/flow cytometer, fluorescence microscopy/fluorescence imaging, fluorescent dyes, live-cell imaging, oxidative stress |
| Scientific poster (2010) | High content imaging of autophagy | antibodies, ArrayScan, autophagy, BacMam technology, cell signaling, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging, microplate reader |
| Scientific poster (2010) | Antibody- and fluorescent protein-based approaches to measuring autophagy in mammalian cells by fluorescence microscopy | Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging |
| Scientific poster (2010) | Autophagosomal accumulation perturbs Golgi structure without affecting other organelles: Implications for autophagosome biogenesis | Alexa Fluor, antibodies, autophagy, BacMam technology, cell structure-golgi, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, live-cell imaging |
| Scientific poster (2010) | A new generation of cellular imaging probes for neurosciences research | autophagy, BacMam technology, CellLight, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels, ion indicators |
| Scientific poster (2010) | New fluorescent probes for live-cell imaging | autophagy, cell cycle, CellLight, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent proteins, live-cell imaging, pH detection, phagocytosis |
| Scientific poster (2011) | Simultaneous analysis of cell death mechanisms and oxidative stress using high content imaging | Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2011) | Primary human cells from Life Technologies: Offerings, scale and applications in HCS and HTS formats | Alexa Fluor, antibodies, autophagy, BacMam technology, cell proliferation, cell signaling, Click-iT, drug discovery, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis |
| Scientific poster (2011) | Validation of high content imaging and analysis assays for cell health and cytotoxicity | antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, cell health, cell proliferation, CellLight, Click-iT, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, pH detection |
| Scientific poster (2011) | The next generation of cell-based imaging assays for apoptosis, autophagy and oxidative stress from Molecular Probes | Alexa Fluor, antibodies, apoptosis, ArrayScan, autophagy, caspase substrates, fluorescence microscopy/fluorescence imaging, fluorescent dyes, high content analysis, microplate reader, oxidative stress |
| Scientific poster (2014) | New fluorescent probes and sensors for visualizing endocytosis, lysosomal dynamics and autophagy | autophagy, cell structure-lysosomes, endocytosis/phagocytosis, autophagy, fluorescence microscopy/fluorescence imaging, high content analysis, microplate reader |
| Scientific poster (2015) | Visualizing the life and death of cells: novel probes and platforms | apoptosis, ArrayScan, autophagy, cell death, CellInsight, fluorescence microscopy/fluorescence imaging, high content analysis |
| Webinar | Basic techniques in autophagy research This webinar will introduce you to a series of analytical tools and techniques to help you identify and interrogate key features of autophagy. Topics to be covered include: Tips and tricks for selecting the right tools and achieving the best results Fluorescent proteins and antibodies used to analyze both live and fixed cells Analysis steps using a variety of multiplexing options, with quantitative methods for image analysis or fluorescence intensity measurement | autophagy, cell health, fluorescence microscopy/fluorescence imaging, fluorescent proteins, high content analysis, immunofluorescence (IF) |
For Research Use Only. Not for use in diagnostic procedures.