Ion Indicators and Ion Channels Information

Learn about assays and techniques for detecting and monitoring ion channels and ion flux using fluorescent ion indicators by flow cytometry, imaging microscopy, high-content analysis and microplate assays. Topics include calcium and magnesium indicators, as well probes for potassium channel flux.

Ion indicators and ion channels features

BioProbes article

FluxOR II Green Potassium Ion Channel Assay—Next-generation detection of potassium ion flux

Potassium channels are ion-selective protein pores that span the cell’s plasma membrane and serve to establish and regulate membrane potential. In excitable cells such as neurons and myocytes, these channels function both to shape the action potential and to reset the cell’s resting membrane potential. The Invitrogen FluxOR II Green Potassium Ion Channel Assay is the newest tool for high-throughput detection of potassium ion channel and transporter activities.

Molecular Probes Handbook
Technical Note

Loading and Calibration of Intracellular Ion Indicators—Note 19.1

This article provides an overview of what you need to know to load ion indicators into living cells and how to calibrate your system to get the most out of the data.

Ion indicators and ion channels learning resources

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Type	Title	Categories
BioProbes articles (Issues 50– present day)	BioProbes Journal of Cell Biology Application	cell analysis, flow cytometry, imaging microscopy, immunoassays, antibodies, protein detection and quantification
Molecular Probes Handbook	MAXC Computer Program for calculating Free Ca2+ concentrations—Note 19.2	ion channels, ion flux, ion indicators
Molecular Probes Handbook	Loading and calibration of intracellular ion indicators—Note 19.1	ion channels, ion flux, ion indicators
Molecular Probes Handbook	Calcium regulation—Section 17.2	ion channels, ion flux, ion indicators
Molecular Probes Handbook	Probes for ion channels and carriers—Section 16.3	ion channels, ion flux, ion indicators
Molecular Probes Handbook	Probes useful at near-neutral pH—Section 20.2	BCECF, flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, pH detection, pH indicators, SNARF
Molecular Probes Handbook	Probes useful at acidic pH—Section 20.3	flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook	pH indicator conjugates—Section 20.4	flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook	Overview of pH indicators—Section 20.1	flow cytometer/flow cytometry, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorometer, live-cell imaging, LysoSensor, pH detection, pH indicators, pHrodo
Molecular Probes Handbook	Fluorescent Na+ and K+ indicators—Section 21.1	ion channels, ion flux, ion indicators
Molecular Probes Handbook	Detecting chloride, phosphate, nitrite and other anions—Section 21.2	ion channels, ion flux, ion indicators
Molecular Probes Handbook	Introduction to Ca2+ measurements with fluorescent indicators—Section 19.1	ion channels, ion flux, ion indicators
Molecular Probes Handbook	Fluorescent Mg2+ indicators—Section 19.6	ion channels, ion flux, ion indicators
Molecular Probes Handbook	Fluorescent indicators for Zn2+ and other metal ions—Section 19.7	ion channels, ion flux, ion indicators
Molecular Probes Handbook	Fluorescent Ca2+ indicators excited with visible light—Section 19.3	ion channels, ion flux, ion indicators
Molecular Probes Handbook	Fluorescent Ca2+ indicators excited with UV light—Section 19.2	ion channels, ion flux, ion indicators
Molecular Probes Handbook	Fluorescent Ca2+ indicator conjugates—Section 19.4	ion channels, ion flux, ion indicators
Molecular Probes Handbook	Chelators, calibration buffers, ionophores and cell-loading reagents—Section 19.8	ion channels, ion flux, ion indicators
Molecular Probes Handbook	Protein-based Ca2+ indicators—Section 19.5	calcium flux, fluorescent dyes, ion indicators
Scientific poster (2008)	Living molecular probes for the next generation	BacMam technology, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, ion flux/ion channels, ion indicators, live-cell imaging
Scientific poster (2008)	Cell-based potassium ion channel screening with the FluxOR Assay	BacMam technology, cell counting, Countess, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2008)	Improving downstream results with automated cell counting	automated cell counter, BacMam technology, cell counting, cell expansion, cell isolation, Countess, fluorescent dyes, hemocytometer, ion flux/ion channels, microplate reader
Scientific poster (2008)	FluxOR, a universal potassium channel assay: Screening BacMam-delivered and stably expressed targets	BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2009)	Cell-based potassium ion channel screening with BacMam using the FluxOR Assay	BacMam technology, drug discovery, fluorescent dyes, high content analysis, ion flux/ion channels, microplate reader
Scientific poster (2010)	A new generation of cellular imaging probes for neurosciences research	autophagy, BacMam technology, CellLight, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels, ion indicators
Scientific poster (2010)	BacMam gene expression for functional and imaging applications	BacMam technology, cell cycle, fluorescence microscopy/fluorescence imaging, fluorescent dyes, fluorescent proteins, gene expression, high content analysis, ion flux/ion channels
Video	LIVE DEAD Cell Imaging kit on the EVOS Auto Imaging System Time course of cell death visualized using the LIVE/DEAD Cell Imaging kit (R37601). The LIVE/DEAD Cell Imaging kit is based on a cell-permeable dye (calcein, AM) that stains live, viable cells bright green and a cell-impermeable red marker that only stains dead and dying cells, which are characterized by compromised cell membranes. Labeled U-2 OS cells were treated 1 μM staurosporine and fluorescence images in the FITC and TexasRed channels were acquired every 5 minutes over 14 h on the EVOS Auto Imaging System using a 20x objective.	cell viability, fluorescence microscopy/fluorescence imaging, ion channels, ion flux, ion indicators, live-cell imaging

For Research Use Only. Not for use in diagnostic procedures.