EVOS M3000 Imaging System

Objectively precise, incredibly easy

The EVOS M3000 Imaging System was designed to be the workhorse of any cell culture or research laboratory by democratizing the ability to produce reliable, stunning high-quality cell images and video. This system automatically and precisely measures cell confluency in real time, with no expertise required. This compact, versatile, easy-to-use system is capable of two-color fluorescence, color-imaging, brightfield, and phase contrast, making it your go-to manual microscope.

Features

In addition to the common features of all EVOS microscopes, the EVOS M3000 Imaging System offers:

Cell confluency in seconds

Built-in automated real-time image analysis for measuring cell confluency

Versatile

Capture images in brightfield, phase contrast, color imaging, and fluorescence

Flexible

Compatible with the full selection of EVOS objectives (1.25-60X) and light cubes

No training required

Simple user interface with touchscreen display

Space-saving design

Our smallest EVOS footprint fits easily on the lab bench or in a cell culture hood

Connectivity

Network-capable instrument facilitates seamless data transfer, storage and collaboration enhancing productivity and data management

EVOS real-time confluency tool

The patent-pending EVOS real-time confluency tool can typically measure confluency in less than 1 second without requiring any image capture, making it excellent for eliminating user bias in routine cell culture. The output from this proprietary bioapplication correlates well with confluency measurements from other software, e.g., Cell Profiler, and is standard on all EVOS M3000 instruments. 

Induced pluripotent stem cells imaged over time with the EVOS M3000 instrument. Human fibroblast-derived induced pluripotent stem cells were cultured on a vitronectin-coated 6-well plate in Gibco Essential 8 Flex Medium for 3 days. Cells were imaged with the EVOS M3000 instrument under phase microscopy with and without the automatically generated confluency mask and measurement.

How accurately can you measure confluency in stem cell culture images?

Put your skills to the test and learn about tools for more consistent and accurate cell confluency measurement in our latest blog.

Accessories

EVOS objectives

Choose from over two dozen EVOS and Olympus 1.25-60X objectives to customize your imaging experience with the EVOS M3000 Imaging System. Objectives can be ordered individually or as one of these two starter bundles.

 

Objective starter kit Kit contents Applications Cat. No. 
EVOS Objective Starter Kit for Brightfield/Phase Contrast

Includes:

 Brightfield & phase contrast AMEP5009
EVOS Objective Starter Kit for Fluorescence/Brightfield/Phase Contrast

Includes:

 Fluorescence, brightfield & phase contrast AMEP5010

Visit EVOS Cell Imaging System Accessories to learn more about the complete objectives available for EVOS Imaging Systems.

EVOS light cubes

EVOS LED-based light cubes provide excellent illumination uniformity with maximal signal-to-background ratios and minimal bleed-through. EVOS light cubes are user-changeable and automatically recognized for plug-and play across the spectrum and across EVOS imaging systems. Light cubes are available individually or as convenient light cube starter kits.

 

Kit contents  Compatible dyes* Cat. No. 

Includes:

GFP: GFP, Alexa Fluor 488, CyQuant Direct

Texas Red: RFP, mCherry, Texas Red, LIVE/DEAD Fixable Red, LysoTracker Red 

 AMEP5015

Includes:

DAPI: DAPI, Hoechst, NucBlue Live

GFP: GFP, Alexa Fluor 488, CyQuant Direct

Texas Red: RFP, mCherry, Texas Red, LIVE/DEAD Fixable Red, LysoTracker Red 

 AMEP5016

Includes:

DAPI: DAPI, Hoechst, NucBlue Live

GFP: GFP, Alexa Fluor 488, CyQuant Direct

 AMEP5018

*This is not a complete list of compatible dyes. 

The Fluorescence SpectraViewer tool is designed to help optimize light cube and dye compatibility for your EVOS or Countess imaging systems. 

EVOS M3000 mechanical stage

The EVOS M3000 Mechanical Stage is an optional addition to the universal, fixed stage provided with the EVOS M3000 Imaging System. This mechanical stage has X-Y axis controls and a vessel holder framework that accommodates our broad range of vessel holders. When using the mechanical stage, vessel holders and large vessels can be held securely in place by a stage clip during operation.

Featured images and videos

FluoCells prepared slide #1 (Cat. No. F36924) showing bovine pulmonary artery endothelial cells (BPAE) labeled with MitoTracker Red CMXRos (Cat. No. M7512) for mitochondria, and then fixed, permeabilized and stained with Alexa Fluor 488 phalloidin (Cat. No. A12379) for F-actin. This two-channel image was acquired on an EVOS M3000 Imaging System using GFP and RFP EVOS Light Cubes (Cat. No. AMEP4951 and AMEP4952) and an Olympus 60X 1.42 NA oil-immersion objective (Cat. No. AMEP4910).

Fixed tumoroids expressing GFP grown in OncoPro Tumoroid Culture Medium Kit (Cat. No. A5701201) on Nunc Cell-Culture Treated Multidishes (Cat. No. 140685). Two-channel image was acquired on an EVOS M3000 Imaging System using an EVOS 20X Objective (Cat.No. AMEP4982) for phase contrast and an EVOS GFP Light Cube (Cat. No. AMEP4951).

Achieve optimal video quality when recording images captured with transmitted light on the EVOS M3000 Imaging System. Shown here are iPSCs recorded using an EVOS 4X Objective (Cat. No. AMEP4980) for phase contrast.

Specifications

 

Attribute
Description
Optics

Imaging modes

Fluorescence, brightfield, color brightfield, and phase contrast 

Imaging methods

Single color, multicolor, and movie capture 

Optical system

Infinity‐corrected optical system; RMS‐threaded objectives with 45 mm parfocal distance
Illumination

Adjustable-intensity transmitted light LED for brightfield, phase contrast, and color imaging applications; optional adjustable-intensity LED light cubes for fluorescence applications (>50,000-hour life per cube)

Contrast methods

Epifluorescence and transmitted light (for brightfield and phase contrast applications)

Light cube capacity

2-position chamber for up to 2 fluorescent LED cubes

Light cubes

Selection from Invitrogen EVOS fluorescent LED cubes, including, but not limited to: 

• DAPI (357/447 nm) 

• GFP (482/524 nm) 

• RFP (542/593 nm) 

• Texas Red (585/628 nm) 

• Cy5 (635/692 nm) 

Motorized LED cube interchange mechanism. Custom LED cubes available on request

Objective capacity

4-position manual turret (not automated)
Objectives

Selection from more than 35 high-quality, long working distance (LWD) and coverslip-corrected (CC) objectives; magnification from 1.25x to 60x 
Condenser

76 mm long working distance condenser; 4-position condenser turret with a clear aperture and 3-phase annuli

Focus mechanism

Manual and touchscreen driven focus with sub-micron (0.15 μm) resolution
Camera High-sensitivity color CMOS camera (2064 × 1536-pixel resolution, 3.2 Megapixels) with 3.45‑µm pixel resolution

Captured images

8-bit monochrome: TIFF, PNG, JPG 

8-bit color: TIFF, PNG, JPG 

Movies: MP4 
LCD display

10.1-inch high-resolution LCD touchscreen display (1920 × 1200-pixel resolution)
Mechanics Stage control

Manual (optional mechanical stage add-on)
Stage size 7.0 x 9.0 in (17.9 x 22.9 cm)
Mechanical stage (optional) range 5.0 x 3.0 in (12.7 x 7.6 cm); optimal for well plates and EVOS vessel holders
Vessels Compatibility

Microscope and chamber slides

Hemocytometers

6-, 12-, 24-, 48-, 96-, and 384-well microplates

35-, 50-, 60-, and 100-mm petri dishes

T-25, T-75, and T-175 flasks

Software and PC

 Integrated onboard operating software

Touchscreen controlled embedded graphical user interface (eGUI)

Confluence measurement with motion detection and sensitivity adjustment over live/captured image

Contrast stretching over live/captured image

Single capture and video recording

Image saving

 Save images on embedded hard drive, an external USB device, a local network drive, Thermo Fisher Connect, or Microsoft OneDrive
Computer Embedded PC with 8 GB RAM
Storage

120 GB SSD, support for USB 3.0 memory stick, network drive and cloud storage
System

Output ports

1 USB 3.0, 2 UB 2.0, 1 DisplayPort

Networking capability

Connection through Windows/SMB network wirelessly using the included Wi-Fi adaptor; wired using USB to Ethernet adapter (not included)

Cloud connectivity

Connect to the Thermo Fisher Connect Platform or Microsoft OneDrive for remote access to images and data via the internet

Power supply

Universal power supply (12 V, 5 A) and power cord

Physical characteristics

 Dimensions (L x W x H)

19.3 x 11.7 x 12.8 in. (49.1 x 29.8 x 32.4 cm)
Weight 

18.4 lb (8.3 kg)

Ordering information

For Research Use Only. Not for use in diagnostic procedures.