Imaging applications

Imaging reagents detecting cell structures

Using fluorescent dyes, probes, and reagents allows researchers to gain insights into various aspects of cellular biology, such as cellular function, organelle structure, and detect proteins, nucleic acids, lipids, and carbohydrates. These fluorescent reagents can be combined with other dyes or antibodies, enabling multiplex analysis. This multiplexing is achieved by selecting appropriate fluorophores that can be excited at distinct wavelengths and using microscopes equipped with suitable filters to separate the emitted fluorescence signals. By leveraging these reagents, researchers can obtain complex and comprehensive information about cellular processes and structures.

Explore fluorescence microscopy reagents and dyes identifying cellular function and organelle structures:

Cell painting assay reagents


Imaging reagents for apoptosis analysis

Various approaches can used to study apoptosis due to the complex and dynamic nature of programmed cell death cascades. There are an array of fluorescent assays and reagents that can be used to detect these apoptotic process. These assays and reagents can be combined with other dyes or antibodies, each excited and emitted at distinct wavelengths. The fluorescent signals can be visualized using microscopes equipped with suitable filters. This multi-parametric approach allows for a comprehensive understanding of the mechanism involved in cell death.

Explore fluorescence microscopy assays and reagents identifying cells undergoing apoptosis:

illustration of the differences in cellular process of a cell undergoing apoptosis versus necrosis

Interested in the non-apoptotic form of cell death? Learn more about Ferroptosis Research Solutions


Fluorophore technology for microscopy experiments

Learn about fluorophore selection and understand how to pick the correct fluorophore technology for your imaging experiments.

Fluorescence spectra of Alexa Fluor dyes

Overview of Alexa Fluor Dyes

Choose Alexa Fluor fluorescent dyes for your imaging experiments by viewing a selection guide and examining spectral information.

 

Learn about Alexa Fluor Dyes

Diagram of tyramide signal amplification mechanism of action

Overview of Tyramide Amplification Kits and Reagents

Having issues detecting your low abundant proteins? Understand how to amplify the signal of fluorescent proteins using the tyramide amplification method. 

Learn about TSA

Diagram of Click-iT EdU reaction

Click-iT EdU Assays for cellular proliferation 

Discover a faster and gentler BrdU alternative to measure cell proliferation with Click-iT EdU assays. Read the selection guide and read the protocol.

Learn about Click-iT EdU Assays

Microscopy protocols

Using fluorescent reagents is often less complex compared to other techniques and are more easily detected. Typical labeling times span between 5 and 60 mins. Certain fluorophore signal can be retained with fixation, and a number can be used for live-cell imaging. The protocols and reagents described in imaging protocol handbook and linked below are to be used as a guide only, and may require optimization for your experiment.

Popular microscopy protocols include:

Imaging tools

Find helpful tools below to help you plan your imaging experiments. 

Resources

  • Cell Imaging Resource Center 
    Access articles, webinars and videos, experimental design, and more for imaging microscopy and high-content analysis
  • Molecular Probes School of Fluorescence
    Learn about fluorescence microscopy, find protocols, and get troubleshooting help for your experiments
  • Imaging Insights
    Read or listen to articles on the latest product innovations, novel techniques, and trending news that educate, inform, and inspire

Posters and guides

Related instruments and brochures

Support

ReadyProbes™ Reagents SpectraViewer

 

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For Research Use Only. Not for use in diagnostic procedures.