NovaFluor Yellow 570 Dye

NovaFluor dyes, including NovaFluor Yellow 590 dye, are built using Phiton technology and are compatible with both spectral flow cytometry as well as traditional flow cytometry. NovaFluor dyes exhibit narrow emission spectra and minimal cross-laser excitation, reducing spectral spillover for better marker resolution. In addition, their unique spectral signatures can provide the opportunity to detect additional markers in flow cytometry panels by opening up previously unusable channels.

NovaFluor Yellow 590 dye has a unique emission spectrum, which fits in between PE and PE-tandems such as PE-Dazzle 594, potentially allowing the detection of an additional marker in a complex multicolour panel.  However due to spectral spillover by PE and PE-tandems, an optimal panel design would replace PE and PE tandems with NovaFluor Yellow 570 and NovaFluor Yellow 610 to reduce spillover into NovaFluor Yellow 590.  NovaFluor Yellow 590 dye should generally be paired with medium to highly expressed antigens to minimize spectral spillover in complex multicolor panels. The macromolecule-based NovaFluor Yellow 590 dye produces highly stable fluorescence, and stained samples retain their fluorescence intensity and spectral signature when stored at 4°C.

Benefits of NovaFluor Yellow 590 dye

  • Narrow emission spectrum and minimal cross-laser excitation for better resolution
  • Should be paired with medium to highly expressed antigens to minimize spillover
  • Can allow the detection of an additional marker between PE and PE-Tandems or NovaFluor Yellow 570 and NovaFluor Yellow 610
  • Produces highly stable fluorescence and is compatible with all buffers tested

Use NovaFluor dyes with CellBlox Monocyte and Macrophage Blocking Buffer to block non-specific binding of NovaFluor labels, PE and APC tandems observed with macrophages and monocytes.

NovaFluor Yellow 590 dye dashboard


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NovaFluor Yellow 590 dye products

We offer NovaFluor dyes conjugated to primary antibodies for use in flow cytometry, as well as, NovaFluor Antibody Conjugation Kits, NovaFluor CD4 Label Characterization Kits, and custom conjugation services.

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Experimental data using NovaFluor Yellow 590 dye products

Figure 1. Absorption and fluorescence emission spectra of NovaFluor Yellow 590 dye.
 

Figure 2. Spectral signature of NovaFluor Yellow 590 dye. Normal human peripheral blood cells stained with anti-CD4 antibodies (clone SK3) conjugated to NovaFluor Yellow 590 dye were used for analysis. Data was acquired on a 5-laser Cytek Aurora system.
Side scatter plot with unstained and CD4 NovaFluor Yellow 590 stained normal human PBMCs

Figure 3. Staining of normal human peripheral blood cells with side scatter and unstained (left) or CD4 Monoclonal Antibody, NovaFluor Yellow 590 (Cat. No.  H001T03Y02) (right). Data was acquired in the YG2 channel on a 5-laser Cytek Aurora system, and singlet cells were used for analysis.