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It was once assumed that cell populations were homogeneous, but the latest evidence shows that heterogeneity does in fact exist even within small cell populations. Gene expression measurements based on the homogenized cell population are misleading averages and don't account for the small but critical changes occurring in individual cells. Individual cells can differ dramatically in size, protein levels, and expressed RNA transcripts, and these variations are key to answering previously unsolvable questions in cancer research, stem cell biology, immunology, developmental biology, and neurology.
We offer analysis tools that can be used throughout various single-cell workflows for cellular biologists and researchers performing a wide spectrum of basic, translational, and clinical research projects.
With single-cell analysis you can:
View our newest application note on single cell analysis
We offer proven workflows using these products for single-cell gene expression:
Figure 1. Single Cell-to-CT real-time PCR workflow.
Download our application note on single cell gene expression heterogeneity
Rare cells or events
| Scarce, precious sample
| Single-cell precision in populations
|
Visualize single cells with florescence imaging accessible to almost every lab and budget
Sensitive cell analysis in a high-throughput benchtop flow cytometer
A validated workflow for gene expression analysis with a standardized platform for studying single cells
Ion semiconductor sequencing makes high-quality sequencing possible on single cells
An extraordinarily gentle method ideal for microdissection of single cells or a small number of cells
Gene expression assays for single cells using real-time PCR on OpenArray technology
Visualize, automatically track, and analyze single cells in 3D time series data
![]() | Video: Less time to real-time PCR with Ambion Cells-to-CT kits Traditionally the first step in real-time PCR is to isolate the RNA. This isolation step can require significant time and involve multiple handling steps—and a risk of sample loss. Ambion Cells-to-CT kits provide a simple and rapid method for preparing cell lysates. | |
![]() | Video: Cells to CT kit demo In this demonstration, Dr. Richard Fekete prepares 96 samples in under 10 minutes with the Cells-to-Cт Kit, comparing this process to traditional spin column-based RNA purification. |
![]() | Video: Less time to real-time PCR with Ambion Cells-to-CT kits Traditionally the first step in real-time PCR is to isolate the RNA. This isolation step can require significant time and involve multiple handling steps—and a risk of sample loss. Ambion Cells-to-CT kits provide a simple and rapid method for preparing cell lysates. | |
![]() | Video: Cells to CT kit demo In this demonstration, Dr. Richard Fekete prepares 96 samples in under 10 minutes with the Cells-to-Cт Kit, comparing this process to traditional spin column-based RNA purification. |
For Research Use Only. Not for use in diagnostic procedures.