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TOP10 cells are the most popular E. coli strain engineered for high transformation efficiency (>1 x 109 cfu/µg). They are suitable for cloning supercoiled DNA and allow stable replication of high-copy number plasmids. TOP10 competent cells have a genotype similar to DH10B, and derivative strains for different applications are available.
One Shot TOP10 Chemically Competent E. coli | MultiShot StripWell TOP10 Chemically Competent E. coli | MultiShot FlexPlate TOP10 Competent Cells | MultiShot TOP10 Chemically Competent E. coli | |
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Best for |
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Type of transformation | Chemical | Chemical | Chemical | Chemical |
Transformation efficiency | >1 x 109 cfu/µg | >1 x 108 cfu/µg | >1 x 108 cfu/µg | >1 x 108 cfu/µg |
Available formats |
TOP10 Electrocomp™ Kit | One Shot TOP10 Electrocomp™ E. coli | One Shot TOP10/P3 Chemically Competent E. coli | TOP10F´ Electrocomp™ Kit | One Shot TOP10F´ Chemically Competent E. coli | |
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Best for |
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Type of transformation | Electroporation | Electroporation | Chemical | Electroporation | Chemical |
Transformation efficiency | >1 x 1010 cfu/µg | >1 x 1010 cfu/µg | >1 x 108 cfu/µg | >1 x 1010 cfu/µg | >1 x 109 cfu/µg |
Available formats |
These competent cells are available under license for use in your services or for use in manufacturing your products for sale. Please contact us at outlicensing@thermofisher.com.
One Shot TOP10 Chemically Competent E. coli | MultiShot StripWell TOP10 Chemically Competent E. coli | MultiShot FlexPlate TOP10 Competent Cells | MultiShot TOP10 Chemically Competent E. coli | |
---|---|---|---|---|
Best for |
|
|
|
|
Type of transformation | Chemical | Chemical | Chemical | Chemical |
Transformation efficiency | >1 x 109 cfu/µg | >1 x 108 cfu/µg | >1 x 108 cfu/µg | >1 x 108 cfu/µg |
Available formats |
TOP10 Electrocomp™ Kit | One Shot TOP10 Electrocomp™ E. coli | One Shot TOP10/P3 Chemically Competent E. coli | TOP10F´ Electrocomp™ Kit | One Shot TOP10F´ Chemically Competent E. coli | |
---|---|---|---|---|---|
Best for |
|
|
|
|
|
Type of transformation | Electroporation | Electroporation | Chemical | Electroporation | Chemical |
Transformation efficiency | >1 x 1010 cfu/µg | >1 x 1010 cfu/µg | >1 x 108 cfu/µg | >1 x 1010 cfu/µg | >1 x 109 cfu/µg |
Available formats |
These competent cells are available under license for use in your services or for use in manufacturing your products for sale. Please contact us at outlicensing@thermofisher.com.
Still not sure which strain to use? Compare TOP10 competent cell genotypes
In addition to competent cells, we offer a broad suite of products for the cloning workflow.
Explore types of bacterial growth media to use for cloning, plasmid DNA preparation, and protein expression.
Learn more about universal-fit electroporation cuvettes for use with competent cells.
Learn more about one-step PCR cloning that combines traditional restriction and ligation cloning.
Gibson Assembly cloning kits provide highly efficient, seamless cloning, enabling the assembly of multiple DNA fragments of varying lengths into any vector.
Have any questions on competent cells or transformation? Click on the resources listed below to access overviews, videos, genotype guides, and educational resources.
Review rich educational content on transformation workflow, troubleshooting, strategies, and selection guides.
Stay up-to-date on industry trends in molecular cloning with on-demand webinars.
Access white papers and technical and application notes on molecular cloning.
Watch instructional videos on troubleshooting tips, how-to videos, and explainers related to molecular cloning.
For Research Use Only. Not for use in diagnostic procedures.