Product box and its contents: 21 tubes of One Shot TOP10 Chemically Competent E. coli, 1 vial of pUC19 DNA, and 1 bottle of SOC medium

What are TOP10 competent cells?

TOP10 cells are the most popular E. coli strain engineered for high transformation efficiency (>1 x 109 cfu/µg). They are suitable for cloning supercoiled DNA and allow stable replication of high-copy number plasmids. TOP10 competent cells have a genotype similar to DH10B, and derivative strains for different applications are available.

Benefits of TOP10 competent cells

  • Broad utility—Suitable for cloning genomic (methylated) mammalian and plant DNA, library construction, or any routine cloning application
  • Multiple formats—One Shot and MultiShot
  • Available as chemically competent and electrocompetent cells
  • Blue-white colony screening

Which TOP10 competent cells are right for you?

TOP10 competent cells for routine cloning

 One Shot TOP10 Chemically Competent E. coliMultiShot StripWell TOP10 Chemically Competent E. coliMultiShot FlexPlate TOP10 Competent Cells MultiShot TOP10 Chemically Competent E. coli
 
Product box and its contents: 21 tubes of One Shot TOP10 Chemically Competent E. coli, 1 vial of pUC19 DNA, and 1 bottle of SOC medium
Product box and its contents: 1 96-tube rack of MultiShot StripWell TOP10 Chemically Competent E. coli, 1 vial of pUC19 DNA, and 2 bottles of SOC medium
Product box and its contents: 1 96-well MultiShot FlexPlate TOP10 Competent Cells, 1 vial of pUC19 DNA, and 2 bottles of SOC medium
Product box and its contents: 5 96-well plates of MultiShot TOP10 Chemically Competent E. coli, 1 vial of pUC19 DNA, and 5 bottles of SOC medium
Best for
  • Enhanced genomic DNA cloning capabilities
  • Convenient single-use format
  • High-throughput applications
  • High-throughput applications
  • Compatible with automated liquid handling systems
  • Microtiter plate format
  • High-throughput applications
  • Compatible with automated liquid handling systems

 

Type of transformationChemicalChemicalChemicalChemical
Transformation efficiency

>1 x 109 cfu/µg>1 x 108 cfu/µg>1 x 108 cfu/µg>1 x 108 cfu/µg
Available formats

Protocol for routine cloning using TOP10 competent cells

TOP10 competent cells for specialized cloning applications

 TOP10 Electrocomp™ KitOne Shot TOP10 Electrocomp™ E. coli
One Shot TOP10/P3 Chemically Competent E. coliTOP10F´ Electrocomp™ KitOne Shot TOP10F´ Chemically Competent E. coli
 
Product box and its contents: 5 tubes of TOP10 Electrocomp E. coli, 1 vial of pUC19 vector, and 2 bottles of SOC medium
Product box and its contents: multiple tubes of One Shot TOP10 Electrocomp E. coli, 1 vial of pUC19 DNA, and 1 bottle of SOC medium
Product box and its contents: 21 tubes of One Shot TOP10/P3 Chemically Competent E. coli, 1 vial of pUC19 vector, and 1 bottle of SOC medium
Product box and its contents: 10 tubes of TOP10F' Electrocomp E. coli, 1 vial of pUC19 plasmid, and 2 bottles of SOC mediumProduct box and its contents: 21 tubes of One Shot TOP10F' Chemically Competent E. coli, 1 vial of pUC19 Plasmid, and 1 bottle of SOC medium
Best for
  • High transformation efficiency is needed (e.g., small DNA amount)
  • High transformation efficiency is needed (e.g., small DNA amount)
  • Single-use format
  • Selection of supF-containing plasmids
  • Carries F' episome for ssDNA isolation 

 

  • Carries F' episome for ssDNA isolation
  • Single-use format
Type of transformationElectroporationElectroporationChemicalElectroporationChemical
Transformation efficiency

>1 x 1010 cfu/µg>1 x 1010 cfu/µg>1 x 108 cfu/µg>1 x 1010 cfu/µg>1 x 109 cfu/µg
Available formats

These competent cells are available under license for use in your services or for use in manufacturing your products for sale. Please contact us at outlicensing@thermofisher.com.

TOP10 competent cells for routine cloning

 One Shot TOP10 Chemically Competent E. coliMultiShot StripWell TOP10 Chemically Competent E. coliMultiShot FlexPlate TOP10 Competent Cells MultiShot TOP10 Chemically Competent E. coli
 
Product box and its contents: 21 tubes of One Shot TOP10 Chemically Competent E. coli, 1 vial of pUC19 DNA, and 1 bottle of SOC medium
Product box and its contents: 1 96-tube rack of MultiShot StripWell TOP10 Chemically Competent E. coli, 1 vial of pUC19 DNA, and 2 bottles of SOC medium
Product box and its contents: 1 96-well MultiShot FlexPlate TOP10 Competent Cells, 1 vial of pUC19 DNA, and 2 bottles of SOC medium
Product box and its contents: 5 96-well plates of MultiShot TOP10 Chemically Competent E. coli, 1 vial of pUC19 DNA, and 5 bottles of SOC medium
Best for
  • Enhanced genomic DNA cloning capabilities
  • Convenient single-use format
  • High-throughput applications
  • High-throughput applications
  • Compatible with automated liquid handling systems
  • Microtiter plate format
  • High-throughput applications
  • Compatible with automated liquid handling systems

 

Type of transformationChemicalChemicalChemicalChemical
Transformation efficiency

>1 x 109 cfu/µg>1 x 108 cfu/µg>1 x 108 cfu/µg>1 x 108 cfu/µg
Available formats

Protocol for routine cloning using TOP10 competent cells

TOP10 competent cells for specialized cloning applications

 TOP10 Electrocomp™ KitOne Shot TOP10 Electrocomp™ E. coli
One Shot TOP10/P3 Chemically Competent E. coliTOP10F´ Electrocomp™ KitOne Shot TOP10F´ Chemically Competent E. coli
 
Product box and its contents: 5 tubes of TOP10 Electrocomp E. coli, 1 vial of pUC19 vector, and 2 bottles of SOC medium
Product box and its contents: multiple tubes of One Shot TOP10 Electrocomp E. coli, 1 vial of pUC19 DNA, and 1 bottle of SOC medium
Product box and its contents: 21 tubes of One Shot TOP10/P3 Chemically Competent E. coli, 1 vial of pUC19 vector, and 1 bottle of SOC medium
Product box and its contents: 10 tubes of TOP10F' Electrocomp E. coli, 1 vial of pUC19 plasmid, and 2 bottles of SOC mediumProduct box and its contents: 21 tubes of One Shot TOP10F' Chemically Competent E. coli, 1 vial of pUC19 Plasmid, and 1 bottle of SOC medium
Best for
  • High transformation efficiency is needed (e.g., small DNA amount)
  • High transformation efficiency is needed (e.g., small DNA amount)
  • Single-use format
  • Selection of supF-containing plasmids
  • Carries F' episome for ssDNA isolation 

 

  • Carries F' episome for ssDNA isolation
  • Single-use format
Type of transformationElectroporationElectroporationChemicalElectroporationChemical
Transformation efficiency

>1 x 1010 cfu/µg>1 x 1010 cfu/µg>1 x 108 cfu/µg>1 x 1010 cfu/µg>1 x 109 cfu/µg
Available formats

These competent cells are available under license for use in your services or for use in manufacturing your products for sale. Please contact us at outlicensing@thermofisher.com.

Still not sure which strain to use? Compare TOP10 competent cell genotypes

Top10 competent cells: Popular citations


Competent cells: Featured products

In addition to competent cells, we offer a broad suite of products for the cloning workflow.

Resources

Have any questions on competent cells or transformation? Click on the resources listed below to access overviews, videos, genotype guides, and educational resources.

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Short articles and digital handbook for cloning applications

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