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Electroporation-competent cells are engineered bacteria that have highly permeable cell membranes for efficient uptake of foreign DNA. Electroporation involves application of electrical pulses across the bacterial membrane, which creates pores and allows genetic material to permeate the membrane. We offer a variety of Invitrogen electrocompetent E. coli cells to reliably clone your target DNA with high efficiency. These competent cells come in convenient packaging options to fulfill your needs for transforming bacteria by electroporation. Standard cuvettes for electroporation can be acquired separately.
Learn about the differences between chemically competent and electrocompetent cells
DH10B strains are suitable for highest transformation efficiency. Competent cells are prepared using proprietary methods to deliver best-in-class performance.
ElectroMAX DH10B Cells | ElectroMAX DH10B T1 Phage-Resistant Competent Cells | MegaX DH10B T1R Electrocomp™ Cells | |
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Best for |
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Type of transformation | Electroporation | Electroporation | Electroporation |
Transformation efficiency | >1 x 1010 cfu/µg | >1 x 1010 cfu/µg | >3 x 1010 cfu/µg |
Available formats |
The TOP10 strains are genetically similar to the DH10B strain and are suitable for general cloning applications.
TOP10 Electrocomp™ Kit | One Shot TOP10 Electrocomp™ E. coli | TOP10F´ Electrocomp™ Kit | |
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Best for |
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Type of transformation | Electroporation | Electroporation | Electroporation |
Transformation efficiency | >1 x 1010 cfu/µg | >1 x 1010 cfu/µg | >1 x 1010 cfu/µg |
Available formats |
ElectroMAX DH5α-E Competent Cells | ElectroMAX DH12S Cells | ElectroMAX Stbl4 Competent Cells | |
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Best for |
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Type of transformation | Electroporation | Electroporation | Electroporation |
Transformation efficiency | >1 x 1010 cfu/µg | >1 x 1010 cfu/µg | >5 x 109 cfu/µg |
Available formats |
DH10B strains are suitable for highest transformation efficiency. Competent cells are prepared using proprietary methods to deliver best-in-class performance.
ElectroMAX DH10B Cells | ElectroMAX DH10B T1 Phage-Resistant Competent Cells | MegaX DH10B T1R Electrocomp™ Cells | |
---|---|---|---|
Best for |
|
|
|
Type of transformation | Electroporation | Electroporation | Electroporation |
Transformation efficiency | >1 x 1010 cfu/µg | >1 x 1010 cfu/µg | >3 x 1010 cfu/µg |
Available formats |
The TOP10 strains are genetically similar to the DH10B strain and are suitable for general cloning applications.
TOP10 Electrocomp™ Kit | One Shot TOP10 Electrocomp™ E. coli | TOP10F´ Electrocomp™ Kit | |
---|---|---|---|
Best for |
|
|
|
Type of transformation | Electroporation | Electroporation | Electroporation |
Transformation efficiency | >1 x 1010 cfu/µg | >1 x 1010 cfu/µg | >1 x 1010 cfu/µg |
Available formats |
ElectroMAX DH5α-E Competent Cells | ElectroMAX DH12S Cells | ElectroMAX Stbl4 Competent Cells | |
---|---|---|---|
Best for |
|
|
|
Type of transformation | Electroporation | Electroporation | Electroporation |
Transformation efficiency | >1 x 1010 cfu/µg | >1 x 1010 cfu/µg | >5 x 109 cfu/µg |
Available formats |
MegaX DH10B T1R Electrocompetent Cells are the highest efficiency electrocompetent cells available (Figure 1). The advantages of MegaX DH10 T1R electrocompetent cells include:
Figure 1. Transformation efficiency comparison of MegaX DH10B T1R Electrocompetent Cells to E. cloni® 10G Supreme Electrocompetent Cells (Lucigen) and Electro Ten-Blue Electroporation-Competent Cells (Agilent) using manufacturer’s recommended protocols. MegaX DH10B T1R consistently outperformed other electrocompetent cells.
Choose the right electroporation cuvette for your strain.
In addition to competent cells, we offer a broad suite of products for the cloning workflow.
Explore types of bacterial growth media to use for cloning, plasmid DNA preparation, and protein expression.
Learn more about universal-fit electroporation cuvettes for use with competent cells.
Learn more about one-step PCR cloning that combines traditional restriction and ligation cloning.
Gibson Assembly cloning kits provide highly efficient, seamless cloning, enabling the assembly of multiple DNA fragments of varying lengths into any vector.
Have any questions on competent cells or transformation? Click on the resources listed below to access overviews, videos, genotype guides, and educational resources.
Review rich educational content on transformation workflow, troubleshooting, strategies, and selection guides.
Stay up-to-date on industry trends in molecular cloning with on-demand webinars.
Access white papers and technical and application notes on molecular cloning.
Watch instructional videos on troubleshooting tips, how-to videos, and explainers related to molecular cloning.