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Cot-1 DNA® Blocking Reagent
Background hybridization due to repetitive DNA sequences is a common problem in microarray assays. This cross-hybridization makes it difficult to identify differentially expressed genes, especially lower-abundant messages. Cot-1 DNA® blocking reagent blocks repetitive DNA sequences and prevents nonspecific hybridization, providing you with cleaner and more sensitive results in your microarray experiments.
FAQ's
What is Cot-1 DNA® blocking reagent?
Cot-1 DNA® blocking reagent is DNA which is predominantly 50 to 300 bp in size, and enriched for repetitive DNA sequences. Cot-1 DNA® blocking reagent is commonly used to block nonspecific hybridization in aCGH microarray experiments and FISH assays.
What advantages does Cot-1 DNA® blocking reagent provide for my aCGH microarray experiments or FISH assays?
Better results are obtained when Cot-1 DNA® blocking reagent is used due to reduction in cross hybridization, which leads to cleaner, more sensitive aCGH microarray experiments and FISH assays.
How does Cot-1 DNA® blocking reagent work?
Cot-1 DNA® blocking reagent blocks repetitive sequences such as SINEs (short interspersed elements), LINEs (long interspersed elements), and sequence homology among members of the same gene family when added to the hybridization solution.
Which Cot-1 DNA® blocking reagent product should I use for my aCGH microarray experiments and FISH assays?
Human Cot-DNA®- Fluorometric QC has been quantitated by fluorometry; this provides more accurate concentration measurements for higher molecular weight products. Mouse Cot-1 DNA® blocking reagent is recommended for experiments when blocking of repetitive mouse DNA is required.