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Scale up clinical research of infectious diseases
Clinical research laboratories around the world can scale up their infectious disease research by automating their nucleic acid extraction workflows. Thermo Fisher Scientific offers a comprehensive range of technologies and solutions for infectious disease research, including sample preparation solutions with bead-based MagMAX chemistry and KingFisher automation instruments that help maximize time and resources. We offer guidance and solutions for research in respiratory, sexually transmitted, blood-borne, and gastrointestinal pathogen extraction workflows.
Nucleic acid purification kits for infectious disease research
MagMAX Prime Viral/Pathogen NA Isolation Kit
Designed to recover RNA and DNA from viral, bacterial, fungal, and parasitic pathogens from a variety of biological samples for downstream applications such as qPCR and NGS.
MagMAX Kit for HIV-1 Dried Blood
Spots
Optimized for effective isolation and purification of nucleic acids to detect HIV-1 from dried blood spots (DBS) or plasma.
MagMAX Viral/Pathogen Nucleic Acid Isolation Kits
Designed for the recovery of RNA and DNA from a broad spectrum of virus and gram-negative bacteria in samples such as blood, swabs, urine, and viral transport media (VTM).
MagMAX Viral/Pathogen Ultra Nucleic Acid Isolation Kit
Optimized further with a proprietary enzyme mix that enables lysis and nucleic acid recovery from some of the toughest pathogens, such as gram-positive bacteria, yeast and fungi.
MagMAX Microbiome Ultra Nucleic Acid Isolation Kit
Optimized for efficient and fast nucleic acid isolation from stool samples, allowing for high-quality, inhibitor-free DNA and RNA to be recovered in under an hour.
Nucleic acid purification instruments
KingFisher Duo Prime
The KingFisher Duo Prime (low/medium throughput) is meant for small labs and individual researchers and processes up to 12 samples per run.
KingFisher Flex
The KingFisher Flex (medium/high throughput), the standalone laboratory workhorse, processes up to 24 or 96 samples per run and is ideal for high-throughput research.
KingFisher Apex
The KingFisher Apex (medium/high throughput) is similar in specifications to the KingFisher Flex, but redesigned with additional capabilities for clinical research, including a barcode scanner, a cooling block, and a UV decontamination lamp.
KingFisher Presto
The fully automated KingFisher Presto (ultrahigh throughput), meant for high-volume labs, also processes up to 96 samples per run, and integrates with a robotic liquid handler.
View workflows by application
Respiratory viral extraction and pathogen research tools
MagMAX and KingFisher products offer a comprehensive solution for respiratory pathogen research, providing sensitive nucleic acid purification and high-throughput pathogen detection. The combined use of Thermo Fisher Scientific technologies can enable researchers to streamline their workflows and improve their results.
Top 4 Tips for improving your respiratory pathogen research
- Use sensitive extraction methods to get high quality input for downstream applications.
- Research a wide variety of respiratory pathogens with one single MagMAX Viral/Pathogen kit.
- Achieve accurate and reproducible results downstream by using optimized protocols and reagents designed to obtain maximum yield and purity for respiratory pathogen nucleic acid extractions.
- Scalable and versatile extraction methods.
Example respiratory prenanalytical and analysis workflow
Receive samples
Compatible with various sample types that include NP swabs, Naso Asp., VTM, BAL, saliva, and more.
Sample preparation
Nucleic acid extraction using the KingFisher Apex system and MagMAX Viral Pathogen Kits*
*See MagMAX Viral Pathogen Kit Selection Guide for kit selection information
Prepare assay plate and run PCR
Respiratory pathogen detection using TruMark assay kits and panels on QuantStudio systems
Analyze and report
Preform data analysis using the Applied Biosystems QuantStudio Design and Analysis Software
Documents
Sexually transmitted infection and urinary pathogen extraction and research
Sexually transmitted infections (STI), urinary tract infections (UTI), and reproductive health research can all be aided by nucleic acid extraction and recovery using MagMAX Viral Pathogen kits. Our technology provides effective lysis and nucleic acid recovery from a wide range of pathogens. The MagMAX Viral/Pathogen Ultra proprietary enzyme mix is optimized for use with Thermo Scientific KingFisher purification systems, helping you streamline the workflow and enable fast, efficient and automated nucleic acid isolation.
- Advanced nucleic acid recovery—The MagMAX Viral/Pathogen Ultra Kit and MagMAX Prime Viral/Pathogen NA Isolation Kit enables lysis and nucleic acid recovery from some of the toughest pathogens, such as gram-positive bacteria, yeast, and fungi.
- Accurate and reproducible downstream results—MagMAX kits are designed with optimized protocols and reagents for maximum yield and purity of sexual and urinary pathogen nucleic acid extractions, helping ensure high-quality input for downstream applications.
- Scalable extraction workflow—KingFisher systems offer a variety of throughput options depending on the instrument model, enabling flexible and adaptable sample preparation workflows.
Example sexually transmitted and urinary pathogen preanalytical and analysis workflow
Receive samples
Utilize biofluid protocols for STI research including vaginal and genital swabs, urine, and more.
Sample preparation
Nucleic acid extraction using the KingFisher Apex system and MagMAX Viral Pathogen Kits*
*See MagMAX Viral Pathogen Kit Selection Guide for kit selection information
Prepare assay plate and run OpenArray
Sexual health and urinary pathogen detection using TruMark OpenArray panels
Prepare assay plate and run real-time PCR
Sexual health and urinary pathogen real-time PCR detection using TruMark assays and kits
Documents
Gastrointestinal pathogen extraction research
The human gastrointestinal tract is filled with fascinating microbes that researchers are discovering and publishing about regularly. MagMAX Microbiome Ultra Nucleic Acid Isolation Kit or MagMAX Prime Viral/Pathogen NA Isolation Kit paired with the KingFisher Purification system can help labs achieve an efficient work stream for gastrointestinal research. With its bead-beating technology for efficient microbe lysis and optimized workflow for quick, inhibitor-free nucleic acid isolation, researchers can recover DNA and RNA from a wide range of microbes with less hands-on time and fast processing.
- Advanced microbe lysis—Bead-beating technology inside the MagMAX Microbiome and MagMAX Prime kits enables efficient microbe lysis from stool samples.
- Accurate and reproducible downstream results—MagMAX kits are designed with optimized protocols and reagents for maximum yield and purity of gastrointestinal pathogen nucleic acid extractions, helping ensure high-quality input for downstream applications.
- Scalable extraction workflow—KingFisher systems offer a variety of throughput options depending on the instrument model, enabling flexible and adaptable sample preparation workflows.
Example gastrointestinal pathogen preanalytical and analysis workflow
Receive samples
Compatible with stool and fecal swab samples with microbiome solutions.
Sample preparation
Nucleic acid extraction using the KingFisher Apex system and MagMAX Kit.
Prepare assay plate and run OpenArray
Gastrointestinal pathogen detection using TruMark OpenArray panels
Prepare assay plate and run real-time PCR
Gastrointestinal pathogen real-time PCR detection using TruMark assays and kits
Documents
Bloodborne pathogen extraction and research
Bloodborne pathogens are microorganisms that can infect the blood stream and potentially lead to diseases including HIV, Hepatitis B and C, Malaria and Syphilis. The MagMAX Viral/Pathogen Nucleic Acid Isolation Kit for HIV-1 Dried Blood Spots is a cost-effective solution for blood-borne pathogen research that enhances testing volumes in remote regions and is easily transported for global health and surveillance.
- Flexible sample types—Research bloodborne pathogens using both DBS and EDTA treated plasma samples.
- Accurate and reproducible downstream results—MagMAX kits are designed with optimized protocols and reagents for maximum yield and purity of bloodborne pathogen, helping ensure high-quality input for downstream applications.
- Scalable extraction workflow—KingFisher systems offer a variety of throughput options depending on the instrument model, enabling flexible and adaptable sample preparation workflows.
Example HIV-1 genotyping workflow
Receive samples
Compatible whole blood, dried blood spots, and plasma for research in bloodborne pathogens.
Sample preparation
Nucleic acid extraction using the KingFisher Apex systems and MagMAX Dried Blood Spots Kit.
Prepare assay plate and run PCR
Bloodborne pathogen detection using TruMark assay kits and panels on QuantStudio systems.
Sequencing
Next generation sequencing with Genexus or GeneStudio Platforms.
Documents
For Research Use Only. Not for use in diagnostic procedures.