Search Thermo Fisher Scientific
Search Thermo Fisher Scientific
Visualize staining your cell without wasting your reagents, antibodies, or time. The Stain-iT Cell Staining Simulator has been designed to assist with all levels of experimental complexity with the goal of helping you generate quality results.
Click the Instrument tab in Stain-iT Cell Staining Simulator.
Knowing your microscope or imaging instrument includes understanding the number of lasers, lamps, light cubes, and filters. Manufacturer web pages typically include information about their models under specifications or features. If you are unable to find your instrument, you can enter the details manually or use the simulated instrument setting. The simulated instrument setting allows you to see all the reagent and dye possibilities.
Custom settings allows you to enter in your specific instrument.
Click the Cell tab in Stain-iT Cell Staining Simulator.
The next step is to enter the fixation status of your sample. The sample preparation directly impacts the reagents and probes used in your experiment. Some reagents are not compatible with fixatives.
Pick the cellular structure or organelle that you want to stain with a dye or probe or antibody.
Product types include reagents and secondary antibodies/labeling kits. There can be multiple reagents that can visualize a given cell structure. Use the SpectraViewer tool to determine which will provide optimal emitted spectrum.
Examine the spectral compatibility of dyes, probes, and reagents with the SpectraViewer. The goal is to reduce spectral overlap among the multiple reagents you’ve chosen.
Hovering your mouse or pointer on the SpectraViewer will show the wavelength selected and the percentage of excitation and emission of a particular fluorophore associated with the reagent.
Click full screen to get a larger view of the spectrum.
Click on the left corner of the SpectraViewer to separate the fluorophores or lasers onto individual plots. This will show more detail on the individual spectrum. Click the far right corner on exit full screen to go back to experimental conditions.
Learn more about reagents and probes against cellular structure, organelles, and cellular functions.
Understand fluorophore spectra, chemical properties, and which instruments or platforms they can be used on with this guide.
Use the SpectraViewer tool to easily compare excitation and emission spectra of fluorophores and reagents.
Easily search for high-quality primary, secondary, and conjugated antibodies for IF, IHC, and other imaging applications.
Use this 5-step webtool to plan your multicolor flow cytometry experiment.
For Research Use Only. Not for use in diagnostic procedures.