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Brilliant Ultra Violet dyes, Brilliant Violet dyes, and Super Bright dyes use polymer technology to absorb energy and emit light signal. These molecules are conjugated to antibodies used in flow cytometry experiments. By incorporating Brilliant Ultra Violet, Brilliant Violet, and Super Bright dyes in their experiments, scientists can take advantage of a broader range of instrument lasers and channels—including UV and Violet—to help reduce compensation necessary in panels with multiple conjugated antibodies. Super Bright dyes, Brilliant Ultra Violet, and Brilliant Violet dyes use similar polymer technologies.
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Contact us for free panel design with experts along with BVs, BUVs, and more
Either the Super Bright Staining Buffer or Brilliant Staining Buffer will work to help resolve polymer dye-to-dye interactions.
Brilliant Ultra Violet dyes and Brilliant Violet dyes are a polymer dye-based technology and compatible with both spectral flow cytometry, as well as traditional flow cytometry. BUVs and BVs are dyes used on flow cytometers equipped with UV (355 nm) and Violet (405 nm) lasers.
Dye | Initial brightness (out of 5 maximum) | Laser (nm) | Excitation max (nm) | Emission max (nm) | Suggested bandpass filter |
---|---|---|---|---|---|
Brilliant Violet 421 | 4 | Violet (405 nm) | 407 | 423 | 450/50 |
Brilliant Violet 480 | 3 | Violet (405 nm) | 440 | 479 | 525/40 |
Brilliant Violet 605 | 4 | Violet (405 nm) | 407 | 605 | 610/20 |
Brilliant Violet 650 | 2 | Violet (405 nm) | 407 | 649 | 660/20, 630 LP |
Brilliant Violet 711 | 4 | Violet (405 nm) | 407 | 713 | 710/50, 690 LP |
Brilliant Violet 786 | 3 | Violet (405 nm) | 407 | 786 | 780/60, 750 LP |
Dye | Initial brightness (out of 5 maximum) | Laser (nm) | Excitation max (nm) | Emission max (nm) | Suggested bandpass filter |
---|---|---|---|---|---|
Brilliant Ultra Violet 395 | 3 | Ultraviolet (355 nm) | 350 | 395 | 379/28 |
Brilliant Ultra Violet 496 | 2 | Ultraviolet (355 nm) | 350 | 496 | 515/30, 450 LP |
Brilliant Ultra Violet 563 | 4 | Ultraviolet (355 nm) | 350 | 564 | Instruments with yellow-green (561 nm) laser: 585/15, 535 LP without yellow-green: 560/40, 535 LP |
Brilliant Ultra Violet 615 | 3 | Ultraviolet (355 nm) | 350 | 616 | 610/20, 595 LP |
Brilliant Ultra Violet 661 | 4 | Ultraviolet (355 nm) | 350 | 660 | 670/25, 630 LP |
Brilliant Ultra Violet 737 | 4 | Ultraviolet (355 nm) | 350 | 737 | 740/35 |
Brilliant Ultra Violet 805 | 3 | Ultraviolet (355 nm) | 350 | 805 | 820/60 or 780/60 |
Insert BUVs, BVs and Super Bight dyes into experiments for analysis on instruments with spectral or conventional instruments.
A handy reference poster featuring the broad range of our dyes and labeling reagents
BRILLIANT VIOLET and BRILLIANT ULTRA VIOLET are trademarks or registered trademarks of Becton, Dickinson and Company or its affiliates, and is used under license. Powered by Sirigen™.
Not for resale. Super Bright Polymer Dyes are sold under license from Becton, Dickinson and Company.